This is a Validated Antibody Database (VAD) review about rat Bub1b, based on 9 published articles (read how Labome selects the articles), using Bub1b antibody in all methods. It is aimed to help Labome visitors find the most suited Bub1b antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Bub1b synonym: mitotic checkpoint serine/threonine-protein kinase BUB1 beta; BUB1B, mitotic checkpoint serine/threonine kinase; budding uninhibited by benzimidazoles 1 beta; budding uninhibited by benzimidazoles 1 homolog beta

Bethyl
rabbit polyclonal
  • immunocytochemistry; human; 1:1000; loading ...; fig 3f
Bethyl Bub1b antibody (Bethyl Laboratories, A300-386A-1) was used in immunocytochemistry on human samples at 1:1000 (fig 3f). J Biol Chem (2018) ncbi
rabbit polyclonal
  • western blot; human; fig 5
Bethyl Bub1b antibody (Bethyl Laboratories, A300-386A) was used in western blot on human samples (fig 5). J Biol Chem (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:1000; fig s4
Bethyl Bub1b antibody (Bethyl, A300-386 A) was used in immunocytochemistry on human samples at 1:1000 (fig s4). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunoprecipitation; human
  • western blot; human
Bethyl Bub1b antibody (Bethyl, A300-386A) was used in immunoprecipitation on human samples and in western blot on human samples . J Biol Chem (2015) ncbi
rabbit polyclonal
  • immunoprecipitation; human; 1:500
In order to explore how Nek2 isoform A affects anaphase-promoting complex/cyclosome containing Cdc20, Bethyl Bub1b antibody (Bethyl, A300-386a) was used in immunoprecipitation on human samples at 1:500. J Cell Sci (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:2000; fig 3
In order to investigate the regulation of SAC primes kinetochore phosphatases, Bethyl Bub1b antibody (Bethyl, A300-386A) was used in immunocytochemistry on human samples at 1:2000 (fig 3). Nat Cell Biol (2014) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 3
Bethyl Bub1b antibody (Bethyl, A300-386A) was used in immunoprecipitation on human samples (fig 3). Nature (2015) ncbi
rabbit polyclonal
  • western blot; human; fig s1c
Bethyl Bub1b antibody (Bethyl, A300-386) was used in western blot on human samples (fig s1c). Proc Natl Acad Sci U S A (2014) ncbi
rabbit polyclonal
  • western blot; human; fig 4a
Bethyl Bub1b antibody (Bethyl, A300-386A) was used in western blot on human samples (fig 4a). Nat Commun (2014) ncbi
Articles Reviewed
  1. Ciossani G, Overlack K, Petrovic A, Huis In t Veld P, Koerner C, Wohlgemuth S, et al. The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases. J Biol Chem. 2018;293:10084-10101 pubmed publisher
  2. Chen X, Stauffer S, Chen Y, Dong J. Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis. J Biol Chem. 2016;291:14761-72 pubmed publisher
  3. Etemad B, Kuijt T, Kops G. Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint. Nat Commun. 2015;6:8987 pubmed publisher
  4. Scialpi F, Mellis D, Ditzel M. EDD, a ubiquitin-protein ligase of the N-end rule pathway, associates with spindle assembly checkpoint components and regulates the mitotic response to nocodazole. J Biol Chem. 2015;290:12585-94 pubmed publisher
  5. Boekhout M, Wolthuis R. Nek2A destruction marks APC/C activation at the prophase-to-prometaphase transition by spindle-checkpoint-restricted Cdc20. J Cell Sci. 2015;128:1639-53 pubmed publisher
  6. Nijenhuis W, Vallardi G, Teixeira A, Kops G, Saurin A. Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 2014;16:1257-64 pubmed publisher
  7. Izawa D, Pines J. The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C. Nature. 2015;517:631-4 pubmed publisher
  8. Han J, Vitre B, Fachinetti D, Cleveland D. Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling. Proc Natl Acad Sci U S A. 2014;111:E4185-93 pubmed publisher
  9. Shandilya J, Toska E, Richard D, Medler K, Roberts S. WT1 interacts with MAD2 and regulates mitotic checkpoint function. Nat Commun. 2014;5:4903 pubmed publisher