This is a Validated Antibody Database (VAD) review about rat Btk, based on 8 published articles (read how Labome selects the articles), using Btk antibody in all methods. It is aimed to help Labome visitors find the most suited Btk antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Btk synonym: tyrosine-protein kinase BTK; Bruton agammaglobulinemia tyrosine kinase

Santa Cruz Biotechnology
mouse monoclonal (E-9)
  • immunocytochemistry; human; 1:400; fig 3
  • western blot; human; 1:700; fig 2
Santa Cruz Biotechnology Btk antibody (Santa Cruz, sc-28387) was used in immunocytochemistry on human samples at 1:400 (fig 3) and in western blot on human samples at 1:700 (fig 2). Oncotarget (2015) ncbi
mouse monoclonal (7F12H4)
  • immunohistochemistry - paraffin section; human; 1:400; fig 1
  • western blot; human; 1:600; fig 1
Santa Cruz Biotechnology Btk antibody (Santa Cruz, SC-81159) was used in immunohistochemistry - paraffin section on human samples at 1:400 (fig 1) and in western blot on human samples at 1:600 (fig 1). Oncotarget (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 6a
Cell Signaling Technology Btk antibody (Cell Signaling, 5082S) was used in western blot on human samples at 1:1000 (fig 6a). Gut (2018) ncbi
rabbit monoclonal (D3H5)
  • western blot; human; loading ...; fig 5d
In order to report that therapeutic antibody-dependent tumor cell killing was FcgammaR-dependent and mediated by macrophages, Cell Signaling Technology Btk antibody (Cell Signalling, 8547) was used in western blot on human samples (fig 5d). Oncogene (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 5d
In order to report that therapeutic antibody-dependent tumor cell killing was FcgammaR-dependent and mediated by macrophages, Cell Signaling Technology Btk antibody (Cell Signalling, 5082) was used in western blot on human samples (fig 5d). Oncogene (2017) ncbi
rabbit monoclonal (D3H5)
  • western blot; human; fig 5b
In order to elucidate how hepatitis C virus infection alters B cell proliferation, Cell Signaling Technology Btk antibody (cell signalling technology, 8547) was used in western blot on human samples (fig 5b). Oncogene (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 5b
In order to elucidate how hepatitis C virus infection alters B cell proliferation, Cell Signaling Technology Btk antibody (cell signalling technology, 5082) was used in western blot on human samples (fig 5b). Oncogene (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 5
Cell Signaling Technology Btk antibody (Cell Signaling Tech, 5082) was used in western blot on human samples at 1:1000 (fig 5). PLoS ONE (2015) ncbi
rabbit monoclonal (D3H5)
  • western blot; rat; fig 5
Cell Signaling Technology Btk antibody (Cell Signaling, 8547) was used in western blot on rat samples (fig 5). Nat Chem Biol (2015) ncbi
rabbit polyclonal
  • western blot; mouse; fig 2b
Cell Signaling Technology Btk antibody (Cell Signaling, 5082) was used in western blot on mouse samples (fig 2b). Nature (2015) ncbi
rabbit monoclonal (D3H5)
  • western blot; mouse; 1:1000; fig 8c
In order to explore a macrophage TLR9-BTK-calcineurin-NFAT signaling pathway involved in impair fungal immunity, Cell Signaling Technology Btk antibody (Cell signaling, 8547) was used in western blot on mouse samples at 1:1000 (fig 8c). EMBO Mol Med (2015) ncbi
Articles Reviewed
  1. Chong I, Aronson L, Bryant H, Gulati A, Campbell J, Elliott R, et al. Mapping genetic vulnerabilities reveals BTK as a novel therapeutic target in oesophageal cancer. Gut. 2018;67:1780-1792 pubmed publisher
  2. Burgess M, Mapp S, Mazzieri R, Cheung C, Chambers L, Mattarollo S, et al. Increased FcγRIIB dominance contributes to the emergence of resistance to therapeutic antibodies in chronic lymphocytic leukaemia patients. Oncogene. 2017;36:2366-2376 pubmed publisher
  3. Dai B, Chen A, Corkum C, Peroutka R, Landon A, Houng S, et al. Hepatitis C virus upregulates B-cell receptor signaling: a novel mechanism for HCV-associated B-cell lymphoproliferative disorders. Oncogene. 2016;35:2979-90 pubmed publisher
  4. Lorkova L, Scigelova M, Arrey T, Vit O, Pospisilova J, Doktorova E, et al. Detailed Functional and Proteomic Characterization of Fludarabine Resistance in Mantle Cell Lymphoma Cells. PLoS ONE. 2015;10:e0135314 pubmed publisher
  5. Zucha M, Wu A, Lee W, Wang L, Lin W, Yuan C, et al. Bruton's tyrosine kinase (Btk) inhibitor ibrutinib suppresses stem-like traits in ovarian cancer. Oncotarget. 2015;6:13255-68 pubmed
  6. Bradshaw J, McFarland J, Paavilainen V, Bisconte A, Tam D, Phan V, et al. Prolonged and tunable residence time using reversible covalent kinase inhibitors. Nat Chem Biol. 2015;11:525-31 pubmed publisher
  7. Chen Z, Shojaee S, Buchner M, Geng H, Lee J, Klemm L, et al. Signalling thresholds and negative B-cell selection in acute lymphoblastic leukaemia. Nature. 2015;521:357-61 pubmed publisher
  8. Herbst S, Shah A, Mazon Moya M, Marzola V, Jensen B, Reed A, et al. Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus. EMBO Mol Med. 2015;7:240-58 pubmed publisher