This is a Validated Antibody Database (VAD) review about rat Axin, based on 5 published articles (read how Labome selects the articles), using Axin antibody in all methods. It is aimed to help Labome visitors find the most suited Axin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Axin synonym: Axin; axin-1; axin-1; GSK-3beta interacting protein rAxin; axis inhibition protein 1; rAxin

Knockout validation
R&D Systems
goat polyclonal
  • western blot knockout validation; human; 1:1250; fig 3-s1
In order to investigate regulatory mechanisms in canonical WNT signaling, R&D Systems Axin antibody (R and D Systems, AF3287) was used in western blot knockout validation on human samples at 1:1250 (fig 3-s1). elife (2016) ncbi
R&D Systems
goat polyclonal
  • western blot; human; 1:3000; loading ...; fig 5d
R&D Systems Axin antibody (R&D, AF3287) was used in western blot on human samples at 1:3000 (fig 5d). Mol Cell (2018) ncbi
goat polyclonal
  • western blot knockout validation; human; 1:1250; fig 3-s1
In order to investigate regulatory mechanisms in canonical WNT signaling, R&D Systems Axin antibody (R and D Systems, AF3287) was used in western blot knockout validation on human samples at 1:1250 (fig 3-s1). elife (2016) ncbi
goat polyclonal
  • western blot; human; loading ...; fig 2b
In order to show that Axin point mutants derail Wnt signaling and promote tumor growth in vivo, R&D Systems Axin antibody (R&D Systems, AF3287) was used in western blot on human samples (fig 2b). Nat Struct Mol Biol (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (C7B12)
  • western blot; human; 1:1000; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology Axin antibody (Cell Signaling, 3323) was used in western blot on human samples at 1:1000 (fig st1). Nat Commun (2016) ncbi
rabbit monoclonal (C7B12)
  • western blot; human; loading ...; fig 6
Cell Signaling Technology Axin antibody (Cell Signaling, C7B12) was used in western blot on human samples (fig 6). Mol Cell Proteomics (2015) ncbi
Articles Reviewed
  1. Ji L, Lu B, Wang Z, Yang Z, Reece Hoyes J, Russ C, et al. Identification of ICAT as an APC Inhibitor, Revealing Wnt-Dependent Inhibition of APC-Axin Interaction. Mol Cell. 2018;72:37-47.e4 pubmed publisher
  2. Lebensohn A, Dubey R, Neitzel L, Tacchelly Benites O, Yang E, Marceau C, et al. Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling. elife. 2016;5: pubmed publisher
  3. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  4. Anvarian Z, Nojima H, Van Kappel E, Madl T, Spit M, Viertler M, et al. Axin cancer mutants form nanoaggregates to rewire the Wnt signaling network. Nat Struct Mol Biol. 2016;23:324-32 pubmed publisher
  5. Traenkle B, Emele F, Anton R, Poetz O, Haeussler R, Maier J, et al. Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells. Mol Cell Proteomics. 2015;14:707-23 pubmed publisher