This is a Validated Antibody Database (VAD) review about rat Aurka, based on 20 published articles (read how Labome selects the articles), using Aurka antibody in all methods. It is aimed to help Labome visitors find the most suited Aurka antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Aurka synonym: ARK-1; Stk6; aurora kinase A; aurora 2; aurora-A; aurora-related kinase 1; aurora/IPL1-related kinase 1; ratAurA; serine/threonine kinase 6; serine/threonine-protein kinase 6; serine/threonine-protein kinase aurora-A

Invitrogen
mouse monoclonal (35C1)
  • western blot; human; 1:1000; fig s5
In order to analyze prevention of centrosome amplification and neoplastic progression by 14-3-3gamma, Invitrogen Aurka antibody (Invitrogen, 458900) was used in western blot on human samples at 1:1000 (fig s5). Sci Rep (2016) ncbi
mouse monoclonal (35C1)
  • western blot; human; fig 3s
In order to examine how histone H3 threonine 118 alters chromosome structure, Invitrogen Aurka antibody (Invitrogen, 45-C8900) was used in western blot on human samples (fig 3s). elife (2016) ncbi
mouse monoclonal (35C1)
  • western blot; mouse
Invitrogen Aurka antibody (Invitrogen, 35C1) was used in western blot on mouse samples . Oncotarget (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (35C1)
  • western blot; human; 1:500; loading ...; fig 6e; S6n; S6i
In order to report FOSL1 as a common transcriptional signature across mutant KRAS cancers of distinct tissue origin, Santa Cruz Biotechnology Aurka antibody (Santa Cruz, sc-56881) was used in western blot on human samples at 1:500 (fig 6e; S6n; S6i). Nat Commun (2017) ncbi
Proteintech Group
rabbit polyclonal
  • immunocytochemistry; human; 1:200
In order to evaluate diagnostic markers for lung cancer with bronchial brushing specimens, Proteintech Group Aurka antibody (Proteintech Group, 10297-1-AP) was used in immunocytochemistry on human samples at 1:200. Cancer Cytopathol (2014) ncbi
Bethyl
rabbit polyclonal
  • immunocytochemistry; mouse; 1:30; loading ...; fig 4a
In order to determine the role of haspin in bipolar spindle assembly, Bethyl Aurka antibody (Bethyl, A300-072A) was used in immunocytochemistry on mouse samples at 1:30 (fig 4a). J Cell Sci (2016) ncbi
BD Biosciences
mouse monoclonal (4/IAK1)
  • western blot; human; 1:1000; loading ...; fig 4d
BD Biosciences Aurka antibody (BD Biosciences, 610938) was used in western blot on human samples at 1:1000 (fig 4d). Nat Commun (2018) ncbi
mouse monoclonal (4/IAK1)
  • western blot; human; loading ...; fig 3A
BD Biosciences Aurka antibody (BD, 610939) was used in western blot on human samples (fig 3A). J Cell Biol (2016) ncbi
mouse monoclonal (4/IAK1)
  • western blot; human; 1:1000; fig 3
In order to compare regulation of a Golgi-activated pool of Src during G2 by centrosomal maturation and Aurora-A recruitment, BD Biosciences Aurka antibody (BD Biosciences, 610,938) was used in western blot on human samples at 1:1000 (fig 3). Nat Commun (2016) ncbi
mouse monoclonal (4/IAK1)
  • western blot; mouse; 1:500; fig 3
In order to characterize a novel population of binucleated mammary epithelial cells and their essential role in lactation, BD Biosciences Aurka antibody (BD, 4/IAK1) was used in western blot on mouse samples at 1:500 (fig 3). Nat Commun (2016) ncbi
mouse monoclonal (4/IAK1)
  • immunoprecipitation; mouse; fig 2e
BD Biosciences Aurka antibody (BD Biosciences, 610938) was used in immunoprecipitation on mouse samples (fig 2e). elife (2016) ncbi
mouse monoclonal (4/IAK1)
  • western blot; mouse
In order to investigate changes to AURKA signaling after treatment with erlotinib/alisertib combination therapy, BD Biosciences Aurka antibody (BD Transduction, 610939) was used in western blot on mouse samples . Front Oncol (2015) ncbi
mouse monoclonal (4/IAK1)
  • western blot; human; 1:1000; fig 1
BD Biosciences Aurka antibody (BD Biosciences, 610939) was used in western blot on human samples at 1:1000 (fig 1). Mol Biol Cell (2015) ncbi
mouse monoclonal (4/IAK1)
  • western blot; human; fig 3
BD Biosciences Aurka antibody (BD Biosciences, 610939) was used in western blot on human samples (fig 3). Oncogene (2016) ncbi
mouse monoclonal (4/IAK1)
  • western blot; human; fig 2
In order to explore how Tpr regulated chromosomal segregation, BD Biosciences Aurka antibody (BD Transduction, 610939) was used in western blot on human samples (fig 2). Cell Cycle (2015) ncbi
mouse monoclonal (4/IAK1)
  • western blot; mouse; 1:1000
BD Biosciences Aurka antibody (BD biosciences, 610938) was used in western blot on mouse samples at 1:1000. Nat Cell Biol (2015) ncbi
mouse monoclonal (4/IAK1)
  • immunocytochemistry; human
In order to elucidate the mechanism by which BI2536 induces defects in cell proliferation of non-small cell lung cancer, BD Biosciences Aurka antibody (BD, 610938) was used in immunocytochemistry on human samples . Cancer Lett (2015) ncbi
mouse monoclonal (4/IAK1)
  • immunoprecipitation; human; fig s10
BD Biosciences Aurka antibody (BD Biosciences, 610938) was used in immunoprecipitation on human samples (fig s10). Nat Commun (2014) ncbi
mouse monoclonal (4/IAK1)
  • western blot; mouse; 1:1000; fig s6
  • western blot; human; 1:1000; fig 7
BD Biosciences Aurka antibody (BD Biosciences, 610938) was used in western blot on mouse samples at 1:1000 (fig s6) and in western blot on human samples at 1:1000 (fig 7). Nat Commun (2014) ncbi
mouse monoclonal (4/IAK1)
  • western blot; human
BD Biosciences Aurka antibody (BD Pharmingen, 610938) was used in western blot on human samples . J Natl Cancer Inst (2014) ncbi
Articles Reviewed
  1. Lin C, Kitagawa M, Tang X, Hou M, Wu J, Qu D, et al. CoA synthase regulates mitotic fidelity via CBP-mediated acetylation. Nat Commun. 2018;9:1039 pubmed publisher
  2. Vallejo A, Perurena N, Guruceaga E, Mazur P, Martínez Canarias S, Zandueta C, et al. An integrative approach unveils FOSL1 as an oncogene vulnerability in KRAS-driven lung and pancreatic cancer. Nat Commun. 2017;8:14294 pubmed publisher
  3. Uematsu K, Okumura F, Tonogai S, Joo Okumura A, Alemayehu D, Nishikimi A, et al. ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation. J Cell Biol. 2016;215:95-106 pubmed
  4. Balboula A, Nguyen A, Gentilello A, Quartuccio S, Drutovic D, Solc P, et al. Haspin kinase regulates microtubule-organizing center clustering and stability through Aurora kinase C in mouse oocytes. J Cell Sci. 2016;129:3648-3660 pubmed
  5. Mukhopadhyay A, Sehgal L, Bose A, Gulvady A, Senapati P, Thorat R, et al. 14-3-3? Prevents Centrosome Amplification and Neoplastic Progression. Sci Rep. 2016;6:26580 pubmed publisher
  6. Barretta M, Spano D, D Ambrosio C, Cervigni R, Scaloni A, Corda D, et al. Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2. Nat Commun. 2016;7:11727 pubmed publisher
  7. Rios A, Fu N, Jamieson P, Pal B, Whitehead L, Nicholas K, et al. Essential role for a novel population of binucleated mammary epithelial cells in lactation. Nat Commun. 2016;7:11400 pubmed publisher
  8. Shin J, Kim T, Kim H, Kim H, Suh M, Lee S, et al. Aurkb/PP1-mediated resetting of Oct4 during the cell cycle determines the identity of embryonic stem cells. elife. 2016;5:e10877 pubmed publisher
  9. Wike C, Graves H, Hawkins R, Gibson M, Ferdinand M, Zhang T, et al. Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis. elife. 2016;5:e11402 pubmed publisher
  10. Nikonova A, Deneka A, Eckman L, Kopp M, Hensley H, Egleston B, et al. Opposing Effects of Inhibitors of Aurora-A and EGFR in Autosomal-Dominant Polycystic Kidney Disease. Front Oncol. 2015;5:228 pubmed publisher
  11. Min M, Mevissen T, De Luca M, Komander D, Lindon C. Efficient APC/C substrate degradation in cells undergoing mitotic exit depends on K11 ubiquitin linkages. Mol Biol Cell. 2015;26:4325-32 pubmed publisher
  12. Park S, Shim J, Park H, Eum D, Park M, Mi Yi J, et al. MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B. Oncogene. 2016;35:1292-301 pubmed publisher
  13. Kobayashi A, Hashizume C, Dowaki T, Wong R. Therapeutic potential of mitotic interaction between the nucleoporin Tpr and aurora kinase A. Cell Cycle. 2015;14:1447-58 pubmed publisher
  14. Bangs F, Schrode N, Hadjantonakis A, Anderson K. Lineage specificity of primary cilia in the mouse embryo. Nat Cell Biol. 2015;17:113-22 pubmed publisher
  15. Rizkallah R, Batsomboon P, Dudley G, Hurt M. Identification of the oncogenic kinase TOPK/PBK as a master mitotic regulator of C2H2 zinc finger proteins. Oncotarget. 2015;6:1446-61 pubmed
  16. Choi M, Kim W, Cheon M, Lee C, Kim J. Polo-like kinase 1 inhibitor BI2536 causes mitotic catastrophe following activation of the spindle assembly checkpoint in non-small cell lung cancer cells. Cancer Lett. 2015;357:591-601 pubmed publisher
  17. Kim M, Kim M, Lee M, Kim C, Lim D. The MST1/2-SAV1 complex of the Hippo pathway promotes ciliogenesis. Nat Commun. 2014;5:5370 pubmed publisher
  18. Liu Y, Jiang Y, Wang B, Hao J, Shang L, Zhang T, et al. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer Cytopathol. 2014;122:833-41 pubmed publisher
  19. Piazzolla D, Palla A, Pantoja C, Canamero M, de Castro I, Ortega S, et al. Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia. Nat Commun. 2014;5:4226 pubmed publisher
  20. Aydin I, Melamed R, Adams S, Castillo Martin M, Demir A, Bryk D, et al. FBXW7 mutations in melanoma and a new therapeutic paradigm. J Natl Cancer Inst. 2014;106:dju107 pubmed publisher