This is a Validated Antibody Database (VAD) review about rat Atp2b2, based on 23 published articles (read how Labome selects the articles), using Atp2b2 antibody in all methods. It is aimed to help Labome visitors find the most suited Atp2b2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Atp2b2 synonym: PMCA2; plasma membrane calcium-transporting ATPase 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase, Ca++ transporting, plasma membrane 2; plasma membrane Ca++-ATPase; plasma membrane calcium ATPase; plasma membrane calcium pump

Invitrogen
rabbit polyclonal
  • immunocytochemistry; human; loading ...
  • western blot; human; loading ...; fig 3a
In order to explore how HER2 signaling contributes to the formation and maintenance of membrane protrusions, Invitrogen Atp2b2 antibody (Thermo Scientific, PA1-915) was used in immunocytochemistry on human samples and in western blot on human samples (fig 3a). PLoS ONE (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 2i
  • immunocytochemistry; human; loading ...; fig 2b
  • western blot; human; loading ...; fig 2i
In order to show that NHERF1 and PMCA2 modulate HER2 signaling and membrane retention in breast cancers, Invitrogen Atp2b2 antibody (Thermo Scientific, PA1-915) was used in immunoprecipitation on human samples (fig 2i), in immunocytochemistry on human samples (fig 2b) and in western blot on human samples (fig 2i). J Biol Chem (2017) ncbi
mouse monoclonal (5F10)
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; tbl 2
In order to examine the effects of retinal progenitor cell proliferation on the spatiotemporal differentiation and synaptic development of rods and bipolar cells in early postnatal gestational lead exposure retinas, Invitrogen Atp2b2 antibody (Affinity Bioreagents, MA3-914) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (tbl 2). Mol Vis (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
In order to propose that PMCA2 is not required for medial nucleus of the trapezoid body neuron survival and investigate other factors that contribute to their survival, Invitrogen Atp2b2 antibody (Affinity Bioreagents, PA1-915) was used in immunohistochemistry - frozen section on mouse samples (fig 1a). J Neurophysiol (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 4e
In order to investigate how sphingomyelin influence neural pathology in a mouse model of Niemann-Pick disease type A, Invitrogen Atp2b2 antibody (ThermoFisher Scientific, PA1-915) was used in western blot on mouse samples (fig 4e). Mol Psychiatry (2017) ncbi
mouse monoclonal (5F10)
  • western blot; mouse; loading ...; fig 3j
In order to investigate how sphingomyelin influence neural pathology in a mouse model of Niemann-Pick disease type A, Invitrogen Atp2b2 antibody (ThermoFisher Scientific, MA3-914) was used in western blot on mouse samples (fig 3j). Mol Psychiatry (2017) ncbi
mouse monoclonal (5F10)
  • western blot; mouse; loading ...; fig 1a
In order to elucidate how SARAF influences the location of STIM1, Invitrogen Atp2b2 antibody (ThermoFisher Scientific, MA3-914) was used in western blot on mouse samples (fig 1a). Channels (Austin) (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:300; fig 1
In order to investigate the regulation of breast cancer cell proliferation and sensitivity to doxorubicin through the calcium pump plasma membrane Ca(2+)-ATPase 2 (PMCA2), Invitrogen Atp2b2 antibody (Thermo Scientific, PA1-915) was used in immunohistochemistry - paraffin section on human samples at 1:300 (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; pig; fig 9
  • western blot; pig; fig 9
In order to characterize a feedforward mechanism at the G protein-coupled estrogen receptor 1 that controls estrogen enhaced linkage in the vascular endothelial calmodulin network, Invitrogen Atp2b2 antibody (Pierce, MA3-914) was used in immunoprecipitation on pig samples (fig 9) and in western blot on pig samples (fig 9). J Biol Chem (2016) ncbi
mouse monoclonal (5F10)
  • western blot; mouse; 1:250; loading ...; fig s8a
In order to elucidate how DWORF activates the SERCA pump, Invitrogen Atp2b2 antibody (Pierce, 5F10) was used in western blot on mouse samples at 1:250 (fig s8a). Science (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 1
In order to study the interactions between PMCA2 and HER2 in breast cancer cells, Invitrogen Atp2b2 antibody (Thermo Scientific, PA1-915) was used in immunocytochemistry on human samples (fig 1) and in western blot on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (5F10)
  • immunohistochemistry; mouse; 1:500; loading ...; fig s5
In order to investigate the effects of cholesterol supplementation in a mouse model of Huntington's disease, Invitrogen Atp2b2 antibody (Thermo Scientific, 5F10) was used in immunohistochemistry on mouse samples at 1:500 (fig s5). EMBO Mol Med (2015) ncbi
mouse monoclonal (5F10)
  • western blot; human; 1:1000; fig 1
In order to investigate the impact of Tmem65 on heart development, Invitrogen Atp2b2 antibody (Thermo Scientific, MA3-914) was used in western blot on human samples at 1:1000 (fig 1). Nat Commun (2015) ncbi
mouse monoclonal (5F10)
  • immunocytochemistry; human; 1:100
  • western blot; human; 1:1000
Invitrogen Atp2b2 antibody (Thermo Scientific, 5F10) was used in immunocytochemistry on human samples at 1:100 and in western blot on human samples at 1:1000. J Biol Chem (2015) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; human; fig 7
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 2
In order to show that PMCA and GPER/GPR30 physically interact and explore how they functionally influence each other, Invitrogen Atp2b2 antibody (Thermo Scientific, MA3-914) was used in immunoprecipitation on human samples (fig 7), in immunocytochemistry on human samples (fig 3) and in western blot on human samples (fig 2). J Biol Chem (2015) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; human; 1:500
  • western blot; human; 1:1000
Invitrogen Atp2b2 antibody (Pierce, 5F10) was used in immunoprecipitation on human samples at 1:500 and in western blot on human samples at 1:1000. Biochim Biophys Acta (2015) ncbi
mouse monoclonal (5F10)
  • immunohistochemistry - paraffin section; human; 1:1000
  • western blot; human; 1:1000
In order to evaluate the effect of preeclampsia on the activity and expression of the ion pump ATPases in syncytiotrophoblast, Invitrogen Atp2b2 antibody (Affinity Bioreagents, 5F10) was used in immunohistochemistry - paraffin section on human samples at 1:1000 and in western blot on human samples at 1:1000. Hypertens Pregnancy (2015) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; rat
  • western blot; rat
Invitrogen Atp2b2 antibody (Thermo Scientific, 5F10) was used in immunoprecipitation on rat samples and in western blot on rat samples . J Biol Chem (2014) ncbi
mouse monoclonal (5F10)
  • western blot; rat; 1:80
In order to examine both rod spherules and cone pedicles for anoctamin 2 expression, Invitrogen Atp2b2 antibody (Thermo Scientific, MA3-914) was used in western blot on rat samples at 1:80. Invest Ophthalmol Vis Sci (2013) ncbi
Abcam
mouse monoclonal (5F10)
  • western blot; mouse; 1:1000; loading ...; fig 6c
Abcam Atp2b2 antibody (Abcam, ab2825) was used in western blot on mouse samples at 1:1000 (fig 6c). Nat Commun (2018) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; human; loading ...; fig 3h
  • western blot; human; 1:1000; loading ...; fig 2f
Abcam Atp2b2 antibody (abcam, ab2825) was used in immunoprecipitation on human samples (fig 3h) and in western blot on human samples at 1:1000 (fig 2f). Oncotarget (2016) ncbi
mouse monoclonal (5F10)
  • western blot; mouse; fig s8
Abcam Atp2b2 antibody (Abcam, ab2825) was used in western blot on mouse samples (fig s8). Front Endocrinol (Lausanne) (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:50
  • western blot; mouse; 1:1000
In order to investigate how serotonin regulates calcium during lactation, Abcam Atp2b2 antibody (Abcam, ab3529) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 and in western blot on mouse samples at 1:1000. PLoS ONE (2014) ncbi
mouse monoclonal (5F10)
  • western blot; cow; 1:250
In order to study mechanisms of calcium homeostasis in bovine corpora lutea, Abcam Atp2b2 antibody (Abcam, ab2825) was used in western blot on cow samples at 1:250. Biol Reprod (2014) ncbi
Articles Reviewed
  1. Kim H, Mun Y, Lee K, Park Y, Park J, Park J, et al. T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells. Nat Commun. 2018;9:3630 pubmed publisher
  2. Jeong J, Kim W, Kim L, VanHouten J, Wysolmerski J. HER2 signaling regulates HER2 localization and membrane retention. PLoS ONE. 2017;12:e0174849 pubmed publisher
  3. Jeong J, VanHouten J, Kim W, Dann P, Sullivan C, Choi J, et al. The scaffolding protein NHERF1 regulates the stability and activity of the tyrosine kinase HER2. J Biol Chem. 2017;292:6555-6568 pubmed publisher
  4. Chaney S, Mukherjee S, Giddabasappa A, Rueda E, Hamilton W, Johnson J, et al. Increased proliferation of late-born retinal progenitor cells by gestational lead exposure delays rod and bipolar cell differentiation. Mol Vis. 2016;22:1468-1489 pubmed
  5. Weatherstone J, Kopp Scheinpflug C, Pilati N, Wang Y, Forsythe I, Rubel E, et al. Maintenance of neuronal size gradient in MNTB requires sound-evoked activity. J Neurophysiol. 2017;117:756-766 pubmed publisher
  6. Pérez Cañamás A, Benvegnù S, Rueda C, Rábano A, Satrústegui J, Ledesma M. Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease. Mol Psychiatry. 2017;22:711-723 pubmed publisher
  7. Albarrán L, Regodón S, Salido G, Lopez J, Rosado J. Role of STIM1 in the surface expression of SARAF. Channels (Austin). 2017;11:84-88 pubmed publisher
  8. Chen J, Wang H, Xu W, Wei S, Li H, Mei Y, et al. Besides an ITIM/SHP-1-dependent pathway, CD22 collaborates with Grb2 and plasma membrane calcium-ATPase in an ITIM/SHP-1-independent pathway of attenuation of Ca2+i signal in B cells. Oncotarget. 2016;7:56129-56146 pubmed publisher
  9. Peters A, Milevskiy M, Lee W, Curry M, Smart C, Saunus J, et al. The calcium pump plasma membrane Ca(2+)-ATPase 2 (PMCA2) regulates breast cancer cell proliferation and sensitivity to doxorubicin. Sci Rep. 2016;6:25505 pubmed publisher
  10. Tran Q, Firkins R, Giles J, Francis S, Matnishian V, Tran P, et al. Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1. J Biol Chem. 2016;291:10805-23 pubmed publisher
  11. Martin B, Chadwick W, Janssens J, Premont R, Schmalzigaug R, Becker K, et al. GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging. Front Endocrinol (Lausanne). 2015;6:191 pubmed publisher
  12. Nelson B, Makarewich C, Anderson D, Winders B, Troupes C, Wu F, et al. A peptide encoded by a transcript annotated as long noncoding RNA enhances SERCA activity in muscle. Science. 2016;351:271-5 pubmed publisher
  13. Jeong J, VanHouten J, Dann P, Kim W, Sullivan C, Yu H, et al. PMCA2 regulates HER2 protein kinase localization and signaling and promotes HER2-mediated breast cancer. Proc Natl Acad Sci U S A. 2016;113:E282-90 pubmed publisher
  14. Valenza M, Chen J, Di Paolo E, Ruozi B, Belletti D, Ferrari Bardile C, et al. Cholesterol-loaded nanoparticles ameliorate synaptic and cognitive function in Huntington's disease mice. EMBO Mol Med. 2015;7:1547-64 pubmed publisher
  15. Sharma P, Abbasi C, Lazic S, Teng A, Wang D, Dubois N, et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun. 2015;6:8391 pubmed publisher
  16. Cali T, Lopreiato R, Shimony J, Vineyard M, Frizzarin M, Zanni G, et al. A Novel Mutation in Isoform 3 of the Plasma Membrane Ca2+ Pump Impairs Cellular Ca2+ Homeostasis in a Patient with Cerebellar Ataxia and Laminin Subunit 1α Mutations. J Biol Chem. 2015;290:16132-41 pubmed publisher
  17. Tran Q, Vermeer M, Burgard M, Hassan A, Giles J. Hetero-oligomeric Complex between the G Protein-coupled Estrogen Receptor 1 and the Plasma Membrane Ca2+-ATPase 4b. J Biol Chem. 2015;290:13293-307 pubmed publisher
  18. Philippe R, Antigny F, Buscaglia P, Norez C, Becq F, Frieden M, et al. SERCA and PMCA pumps contribute to the deregulation of Ca2+ homeostasis in human CF epithelial cells. Biochim Biophys Acta. 2015;1853:892-903 pubmed publisher
  19. Abad C, Vallejos C, de Gregorio N, Díaz P, Chiarello D, Mendoza M, et al. Na⁺, K⁺-ATPase and Ca²⁺-ATPase activities in basal and microvillous syncytiotrophoblast membranes from preeclamptic human term placenta. Hypertens Pregnancy. 2015;34:65-79 pubmed publisher
  20. de Juan Sanz J, Núñez E, Zafra F, Berrocal M, Corbacho I, Ibáñez I, et al. Presynaptic control of glycine transporter 2 (GlyT2) by physical and functional association with plasma membrane Ca2+-ATPase (PMCA) and Na+-Ca2+ exchanger (NCX). J Biol Chem. 2014;289:34308-24 pubmed publisher
  21. Laporta J, Keil K, Vezina C, Hernandez L. Peripheral serotonin regulates maternal calcium trafficking in mammary epithelial cells during lactation in mice. PLoS ONE. 2014;9:e110190 pubmed publisher
  22. Wright M, Bowdridge E, McDermott E, Richardson S, Scheidler J, Syed Q, et al. Mechanisms of intracellular calcium homeostasis in developing and mature bovine corpora lutea. Biol Reprod. 2014;90:55 pubmed publisher
  23. Dauner K, Möbus C, Frings S, Möhrlen F. Targeted expression of anoctamin calcium-activated chloride channels in rod photoreceptor terminals of the rodent retina. Invest Ophthalmol Vis Sci. 2013;54:3126-36 pubmed publisher