This is a Validated Antibody Database (VAD) review about rat Atp2b1, based on 17 published articles (read how Labome selects the articles), using Atp2b1 antibody in all methods. It is aimed to help Labome visitors find the most suited Atp2b1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Atp2b1 synonym: Pmca1a; Pmca1b; Pmca1c; plasma membrane calcium-transporting ATPase 1; ATPase, Ca++ transporting, plasma membrane 1; PMCA1; plasma membrane calcium ATPase; plasma membrane calcium pump

Invitrogen
mouse monoclonal (5F10)
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; tbl 2
In order to examine the effects of retinal progenitor cell proliferation on the spatiotemporal differentiation and synaptic development of rods and bipolar cells in early postnatal gestational lead exposure retinas, Invitrogen Atp2b1 antibody (Affinity Bioreagents, MA3-914) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (tbl 2). Mol Vis (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1a
In order to explore how the crosstalk between calcium signaling and the MAPK pathway through the regulation of PMCA4b regulates metastasis, Invitrogen Atp2b1 antibody (Affinity BioReagents, PA1-914) was used in western blot on human samples at 1:1000 (fig 1a). Int J Cancer (2017) ncbi
mouse monoclonal (5F10)
  • western blot; mouse; loading ...; fig 3j
In order to investigate how sphingomyelin influence neural pathology in a mouse model of Niemann-Pick disease type A, Invitrogen Atp2b1 antibody (ThermoFisher Scientific, MA3-914) was used in western blot on mouse samples (fig 3j). Mol Psychiatry (2017) ncbi
mouse monoclonal (5F10)
  • western blot; mouse; loading ...; fig 1a
In order to elucidate how SARAF influences the location of STIM1, Invitrogen Atp2b1 antibody (ThermoFisher Scientific, MA3-914) was used in western blot on mouse samples (fig 1a). Channels (Austin) (2017) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; pig; fig 9
  • western blot; pig; fig 9
In order to characterize a feedforward mechanism at the G protein-coupled estrogen receptor 1 that controls estrogen enhaced linkage in the vascular endothelial calmodulin network, Invitrogen Atp2b1 antibody (Pierce, MA3-914) was used in immunoprecipitation on pig samples (fig 9) and in western blot on pig samples (fig 9). J Biol Chem (2016) ncbi
mouse monoclonal (5F10)
  • western blot; mouse; 1:250; loading ...; fig s8a
In order to elucidate how DWORF activates the SERCA pump, Invitrogen Atp2b1 antibody (Pierce, 5F10) was used in western blot on mouse samples at 1:250 (fig s8a). Science (2016) ncbi
mouse monoclonal (5F10)
  • immunohistochemistry; mouse; 1:500; loading ...; fig s5
In order to investigate the effects of cholesterol supplementation in a mouse model of Huntington's disease, Invitrogen Atp2b1 antibody (Thermo Scientific, 5F10) was used in immunohistochemistry on mouse samples at 1:500 (fig s5). EMBO Mol Med (2015) ncbi
mouse monoclonal (5F10)
  • western blot; human; 1:1000; fig 1
In order to investigate the impact of Tmem65 on heart development, Invitrogen Atp2b1 antibody (Thermo Scientific, MA3-914) was used in western blot on human samples at 1:1000 (fig 1). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 8
In order to study alteration of firing properties of mouse retinal ganglion cells due to the gain-of-function nature of Cav1.4 L-type calcium channels, Invitrogen Atp2b1 antibody (ThermoScientific, PA1?C914) was used in immunohistochemistry on mouse samples at 1:100 (fig 8). Channels (Austin) (2015) ncbi
mouse monoclonal (5F10)
  • immunocytochemistry; human; 1:100
  • western blot; human; 1:1000
Invitrogen Atp2b1 antibody (Thermo Scientific, 5F10) was used in immunocytochemistry on human samples at 1:100 and in western blot on human samples at 1:1000. J Biol Chem (2015) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; human; fig 7
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 2
In order to show that PMCA and GPER/GPR30 physically interact and explore how they functionally influence each other, Invitrogen Atp2b1 antibody (Thermo Scientific, MA3-914) was used in immunoprecipitation on human samples (fig 7), in immunocytochemistry on human samples (fig 3) and in western blot on human samples (fig 2). J Biol Chem (2015) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; human; 1:500
  • western blot; human; 1:1000
Invitrogen Atp2b1 antibody (Pierce, 5F10) was used in immunoprecipitation on human samples at 1:500 and in western blot on human samples at 1:1000. Biochim Biophys Acta (2015) ncbi
mouse monoclonal (5F10)
  • immunohistochemistry - paraffin section; human; 1:1000
  • western blot; human; 1:1000
In order to evaluate the effect of preeclampsia on the activity and expression of the ion pump ATPases in syncytiotrophoblast, Invitrogen Atp2b1 antibody (Affinity Bioreagents, 5F10) was used in immunohistochemistry - paraffin section on human samples at 1:1000 and in western blot on human samples at 1:1000. Hypertens Pregnancy (2015) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; rat
  • western blot; rat
Invitrogen Atp2b1 antibody (Thermo Scientific, 5F10) was used in immunoprecipitation on rat samples and in western blot on rat samples . J Biol Chem (2014) ncbi
mouse monoclonal (5F10)
  • western blot; rat; 1:80
In order to examine both rod spherules and cone pedicles for anoctamin 2 expression, Invitrogen Atp2b1 antibody (Thermo Scientific, MA3-914) was used in western blot on rat samples at 1:80. Invest Ophthalmol Vis Sci (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (5F10)
  • immunoprecipitation; mouse; loading ...; fig 1d
Santa Cruz Biotechnology Atp2b1 antibody (Santa Cruz Biotechnology Inc, sc-20028) was used in immunoprecipitation on mouse samples (fig 1d). J Cell Physiol (2018) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; human; fig 7
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 2
In order to show that PMCA and GPER/GPR30 physically interact and explore how they functionally influence each other, Santa Cruz Biotechnology Atp2b1 antibody (Santa Cruz Biotechnology, SC-20028) was used in immunoprecipitation on human samples (fig 7), in immunocytochemistry on human samples (fig 3) and in western blot on human samples (fig 2). J Biol Chem (2015) ncbi
mouse monoclonal (5F10)
  • immunoprecipitation; human
  • immunocytochemistry; mouse; 1:200
  • western blot; mouse; 1:500
Santa Cruz Biotechnology Atp2b1 antibody (Santa Cruz Biotechnology, 5F10) was used in immunoprecipitation on human samples , in immunocytochemistry on mouse samples at 1:200 and in western blot on mouse samples at 1:500. J Biol Chem (2014) ncbi
Articles Reviewed
  1. Olli K, Li K, Galileo D, Martin DeLeon P. Plasma membrane calcium ATPase 4 (PMCA4) co-ordinates calcium and nitric oxide signaling in regulating murine sperm functional activity. J Cell Physiol. 2018;233:11-22 pubmed publisher
  2. Chaney S, Mukherjee S, Giddabasappa A, Rueda E, Hamilton W, Johnson J, et al. Increased proliferation of late-born retinal progenitor cells by gestational lead exposure delays rod and bipolar cell differentiation. Mol Vis. 2016;22:1468-1489 pubmed
  3. Hegedüs L, Garay T, Molnar E, Varga K, Bilecz A, Torok S, et al. The plasma membrane Ca2+ pump PMCA4b inhibits the migratory and metastatic activity of BRAF mutant melanoma cells. Int J Cancer. 2017;140:2758-2770 pubmed publisher
  4. Pérez Cañamás A, Benvegnù S, Rueda C, Rábano A, Satrústegui J, Ledesma M. Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease. Mol Psychiatry. 2017;22:711-723 pubmed publisher
  5. Albarrán L, Regodón S, Salido G, Lopez J, Rosado J. Role of STIM1 in the surface expression of SARAF. Channels (Austin). 2017;11:84-88 pubmed publisher
  6. Tran Q, Firkins R, Giles J, Francis S, Matnishian V, Tran P, et al. Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1. J Biol Chem. 2016;291:10805-23 pubmed publisher
  7. Nelson B, Makarewich C, Anderson D, Winders B, Troupes C, Wu F, et al. A peptide encoded by a transcript annotated as long noncoding RNA enhances SERCA activity in muscle. Science. 2016;351:271-5 pubmed publisher
  8. Valenza M, Chen J, Di Paolo E, Ruozi B, Belletti D, Ferrari Bardile C, et al. Cholesterol-loaded nanoparticles ameliorate synaptic and cognitive function in Huntington's disease mice. EMBO Mol Med. 2015;7:1547-64 pubmed publisher
  9. Sharma P, Abbasi C, Lazic S, Teng A, Wang D, Dubois N, et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun. 2015;6:8391 pubmed publisher
  10. Knoflach D, Schicker K, Glösmann M, Koschak A. Gain-of-function nature of Cav1.4 L-type calcium channels alters firing properties of mouse retinal ganglion cells. Channels (Austin). 2015;9:298-306 pubmed publisher
  11. Calì T, Lopreiato R, Shimony J, Vineyard M, Frizzarin M, Zanni G, et al. A Novel Mutation in Isoform 3 of the Plasma Membrane Ca2+ Pump Impairs Cellular Ca2+ Homeostasis in a Patient with Cerebellar Ataxia and Laminin Subunit 1α Mutations. J Biol Chem. 2015;290:16132-41 pubmed publisher
  12. Tran Q, Vermeer M, Burgard M, Hassan A, Giles J. Hetero-oligomeric Complex between the G Protein-coupled Estrogen Receptor 1 and the Plasma Membrane Ca2+-ATPase 4b. J Biol Chem. 2015;290:13293-307 pubmed publisher
  13. Philippe R, Antigny F, Buscaglia P, Norez C, Becq F, Frieden M, et al. SERCA and PMCA pumps contribute to the deregulation of Ca2+ homeostasis in human CF epithelial cells. Biochim Biophys Acta. 2015;1853:892-903 pubmed publisher
  14. Abad C, Vallejos C, de Gregorio N, Díaz P, Chiarello D, Mendoza M, et al. Na⁺, K⁺-ATPase and Ca²⁺-ATPase activities in basal and microvillous syncytiotrophoblast membranes from preeclamptic human term placenta. Hypertens Pregnancy. 2015;34:65-79 pubmed publisher
  15. de Juan Sanz J, Núñez E, Zafra F, Berrocal M, Corbacho I, Ibáñez I, et al. Presynaptic control of glycine transporter 2 (GlyT2) by physical and functional association with plasma membrane Ca2+-ATPase (PMCA) and Na+-Ca2+ exchanger (NCX). J Biol Chem. 2014;289:34308-24 pubmed publisher
  16. Yang Y, Lee J, Jo H, Park S, Chang I, Muallem S, et al. Homer2 protein regulates plasma membrane Ca²?-ATPase-mediated Ca²? signaling in mouse parotid gland acinar cells. J Biol Chem. 2014;289:24971-9 pubmed publisher
  17. Dauner K, Möbus C, Frings S, Möhrlen F. Targeted expression of anoctamin calcium-activated chloride channels in rod photoreceptor terminals of the rodent retina. Invest Ophthalmol Vis Sci. 2013;54:3126-36 pubmed publisher