This is a Validated Antibody Database (VAD) review about rat Atg12, based on 19 published articles (read how Labome selects the articles), using Atg12 antibody in all methods. It is aimed to help Labome visitors find the most suited Atg12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Atg12 synonym: Apg12l; ubiquitin-like protein ATG12; ATG12 autophagy related 12 homolog; autophagy 12-like; autophagy-related protein 12

Abcam
rabbit polyclonal
  • immunocytochemistry; mouse; 1:400; loading ...; fig 2b
Abcam Atg12 antibody (Abcam, ab155589) was used in immunocytochemistry on mouse samples at 1:400 (fig 2b). J Biol Chem (2018) ncbi
Cell Signaling Technology
rabbit monoclonal (D88H11)
  • western blot; mouse; 1:1000; loading ...; fig 8c
Cell Signaling Technology Atg12 antibody (Cell Signaling Technologies, 4180) was used in western blot on mouse samples at 1:1000 (fig 8c). Sci Rep (2017) ncbi
rabbit monoclonal (D88H11)
  • western blot; mouse; loading ...; fig 5a
Cell Signaling Technology Atg12 antibody (CST, 4180) was used in western blot on mouse samples (fig 5a). Proteomics (2017) ncbi
rabbit monoclonal (D88H11)
  • western blot; human; 1:1000; loading ...; fig 1d
Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180) was used in western blot on human samples at 1:1000 (fig 1d). Autophagy (2016) ncbi
rabbit monoclonal (D88H11)
  • western blot; mouse; loading ...; fig s8c
Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180) was used in western blot on mouse samples (fig s8c). Nature (2016) ncbi
rabbit monoclonal (D88H11)
  • western blot; mouse; fig 5a
In order to research the effects of environmental tobacco smoke on autophagy and longevity, Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180) was used in western blot on mouse samples (fig 5a). Oncotarget (2016) ncbi
rabbit monoclonal (D88H11)
Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180) was used . elife (2016) ncbi
rabbit monoclonal (D88H11)
  • immunocytochemistry; human; 1:1000; fig 1
In order to investigate impairment of autophagy flux and induction of cell death independent of necroptosis and apoptosis by dual PI-3 kinase/mTOR inhibition, Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180) was used in immunocytochemistry on human samples at 1:1000 (fig 1). Oncotarget (2016) ncbi
rabbit monoclonal (D88H11)
  • western blot; human; 1:5000; fig 1
In order to discuss how mutations in superoxide dismutase-1 affect the motor system, Cell Signaling Technology Atg12 antibody (Cell Signaling Technology, 4180) was used in western blot on human samples at 1:5000 (fig 1). Acta Neuropathol Commun (2016) ncbi
rabbit monoclonal (D88H11)
  • western blot; mouse; fig 5
Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180) was used in western blot on mouse samples (fig 5). Autophagy (2015) ncbi
rabbit monoclonal (D88H11)
  • western blot; human; 1:1000; fig s1
In order to determine how starvation-induced autophagy is promoted by transcriptional regulation of Annexin A2, Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180S) was used in western blot on human samples at 1:1000 (fig s1). Nat Commun (2015) ncbi
rabbit monoclonal (D88H11)
  • western blot; rat
  • immunocytochemistry; human
Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180) was used in western blot on rat samples and in immunocytochemistry on human samples . Toxicol Lett (2015) ncbi
rabbit monoclonal (D88H11)
  • western blot; human; fig 3a
Cell Signaling Technology Atg12 antibody (Cell Signaling Technology, 4180) was used in western blot on human samples (fig 3a). Autophagy (2016) ncbi
rabbit monoclonal (D88H11)
  • western blot; rat; loading ...; fig 5a
Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180) was used in western blot on rat samples (fig 5a). PLoS ONE (2015) ncbi
rabbit monoclonal (D88H11)
  • western blot; rat; 1:1000
Cell Signaling Technology Atg12 antibody (Cell Signaling Technology, 4180) was used in western blot on rat samples at 1:1000. Neurobiol Dis (2015) ncbi
rabbit monoclonal (D88H11)
  • western blot; human; fig 7
  • immunohistochemistry - frozen section; mouse; 1:100; fig 9
In order to test if autophagic vacuoles formation is required for Abeta production by SUMO1, Cell Signaling Technology Atg12 antibody (Cell Signaling Technology, 4180) was used in western blot on human samples (fig 7) and in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 9). Autophagy (2015) ncbi
rabbit monoclonal (D88H11)
  • western blot; human; 1:1000
Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180P) was used in western blot on human samples at 1:1000. Biochim Biophys Acta (2015) ncbi
rabbit monoclonal (D88H11)
  • western blot; human; 1:1000
Cell Signaling Technology Atg12 antibody (Cell Signaling Technology, 4180) was used in western blot on human samples at 1:1000. Biochim Biophys Acta (2015) ncbi
rabbit monoclonal (D88H11)
  • western blot; human; 1:1000
Cell Signaling Technology Atg12 antibody (Cell Signaling, 4180P) was used in western blot on human samples at 1:1000. Biochim Biophys Acta (2014) ncbi
Articles Reviewed
  1. Bansal M, Moharir S, Sailasree S, Sirohi K, Sudhakar C, Sarathi D, et al. Optineurin promotes autophagosome formation by recruiting the autophagy-related Atg12-5-16L1 complex to phagophores containing the Wipi2 protein. J Biol Chem. 2018;293:132-147 pubmed publisher
  2. Liu C, Choi H, Johnson Z, Tian J, Shapiro I, Risbud M. Lack of evidence for involvement of TonEBP and hyperosmotic stimulus in induction of autophagy in the nucleus pulposus. Sci Rep. 2017;7:4543 pubmed publisher
  3. Zhong L, Zhou J, Chen X, Liu J, Liu Z, Chen Y, et al. Quantitative proteomics reveals EVA1A-related proteins involved in neuronal differentiation. Proteomics. 2017;17: pubmed publisher
  4. Choi H, Merceron C, Mangiavini L, Seifert E, Schipani E, Shapiro I, et al. Hypoxia promotes noncanonical autophagy in nucleus pulposus cells independent of MTOR and HIF1A signaling. Autophagy. 2016;12:1631-46 pubmed publisher
  5. Shin H, Kim H, Oh S, Lee J, Kee M, Ko H, et al. AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Nature. 2016;534:553-7 pubmed publisher
  6. Ting W, Yang J, Kuo C, Xiao Z, Lu X, Yeh Y, et al. Environmental tobacco smoke increases autophagic effects but decreases longevity associated with Sirt-1 protein expression in young C57BL mice hearts. Oncotarget. 2016;7:39017-39025 pubmed publisher
  7. Yeo S, Itahana Y, Guo A, Han R, Iwamoto K, Nguyen H, et al. Transglutaminase 2 contributes to a TP53-induced autophagy program to prevent oncogenic transformation. elife. 2016;5:e07101 pubmed publisher
  8. Button R, Vincent J, Strang C, Luo S. Dual PI-3 kinase/mTOR inhibition impairs autophagy flux and induces cell death independent of apoptosis and necroptosis. Oncotarget. 2016;7:5157-75 pubmed publisher
  9. Tokuda E, Brännström T, Andersen P, Marklund S. Low autophagy capacity implicated in motor system vulnerability to mutant superoxide dismutase. Acta Neuropathol Commun. 2016;4:6 pubmed publisher
  10. Haim Y, Bluher M, Slutsky N, Goldstein N, Kloting N, Harman Boehm I, et al. Elevated autophagy gene expression in adipose tissue of obese humans: A potential non-cell-cycle-dependent function of E2F1. Autophagy. 2015;11:2074-2088 pubmed publisher
  11. Moreau K, Ghislat G, Hochfeld W, Renna M, Zavodszky E, Runwal G, et al. Transcriptional regulation of Annexin A2 promotes starvation-induced autophagy. Nat Commun. 2015;6:8045 pubmed publisher
  12. Zhang L, Wang H, Ding K, Xu J. FTY720 induces autophagy-related apoptosis and necroptosis in human glioblastoma cells. Toxicol Lett. 2015;236:43-59 pubmed publisher
  13. Luo T, Fu J, Xu A, Su B, Ren Y, Li N, et al. PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression. Autophagy. 2016;12:1355-71 pubmed publisher
  14. McMillan E, Paré M, Baechler B, Graham D, Rush J, Quadrilatero J. Autophagic signaling and proteolytic enzyme activity in cardiac and skeletal muscle of spontaneously hypertensive rats following chronic aerobic exercise. PLoS ONE. 2015;10:e0119382 pubmed publisher
  15. Harris White M, Ferbas K, Johnson M, Eslami P, Poteshkina A, Venkova K, et al. A cell-penetrating ester of the neural metabolite lanthionine ketimine stimulates autophagy through the mTORC1 pathway: Evidence for a mechanism of action with pharmacological implications for neurodegenerative pathologies. Neurobiol Dis. 2015;84:60-8 pubmed publisher
  16. Cho S, Yun S, Jo C, Lee D, Choi K, Song J, et al. SUMO1 promotes Aβ production via the modulation of autophagy. Autophagy. 2015;11:100-12 pubmed publisher
  17. Porter K, Hirt J, Stamer W, Liton P. Autophagic dysregulation in glaucomatous trabecular meshwork cells. Biochim Biophys Acta. 2015;1852:379-85 pubmed publisher
  18. Kim T, Kim H, Kang Y, Yoon S, Lee J, Choi W, et al. Psammaplin A induces Sirtuin 1-dependent autophagic cell death in doxorubicin-resistant MCF-7/adr human breast cancer cells and xenografts. Biochim Biophys Acta. 2015;1850:401-10 pubmed publisher
  19. Porter K, Jeyabalan N, Liton P. MTOR-independent induction of autophagy in trabecular meshwork cells subjected to biaxial stretch. Biochim Biophys Acta. 2014;1843:1054-62 pubmed publisher