This is a Validated Antibody Database (VAD) review about rat Arf1, based on 10 published articles (read how Labome selects the articles), using Arf1 antibody in all methods. It is aimed to help Labome visitors find the most suited Arf1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Arf1 synonym: ADP-ribosylation factor 1

Santa Cruz Biotechnology
mouse monoclonal (ARFS 1A9/5)
  • western blot; mouse; 1:50; loading ...; fig 1d
Santa Cruz Biotechnology Arf1 antibody (SantaCruz, sc-53168) was used in western blot on mouse samples at 1:50 (fig 1d). Sci Adv (2018) ncbi
mouse monoclonal (ARFS 1A9/5)
  • western blot; human; fig 9j
Santa Cruz Biotechnology Arf1 antibody (Santa Cruz, sc-53168) was used in western blot on human samples (fig 9j). Arch Biochem Biophys (2017) ncbi
mouse monoclonal (ARFS 1A9/5)
  • western blot; human; 1:500; loading ...; fig s3a
In order to study the role of TRAPPC13 in autophagy and the cellular response to Golgi stress, Santa Cruz Biotechnology Arf1 antibody (SantaCruz, sc-53168) was used in western blot on human samples at 1:500 (fig s3a). J Cell Sci (2017) ncbi
mouse monoclonal (ARFS 1A9/5)
  • western blot; human; 1:500; loading ...; fig 10c
In order to report that cool-associated tyrosine phosphorylated protein-1 is required for anchorage-independent growth of cervical carcinoma cells, Santa Cruz Biotechnology Arf1 antibody (Santa Cruz, sc-53168) was used in western blot on human samples at 1:500 (fig 10c). J Biol Chem (2017) ncbi
mouse monoclonal (ARFS 1A9/5)
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 5
In order to report that PKD2 controls secretion of matrix metalloproteinase cargo, Santa Cruz Biotechnology Arf1 antibody (Santa Cruz Biotechnology, sc-53168) was used in immunocytochemistry on human samples (fig 2) and in western blot on human samples (fig 5). J Biol Chem (2016) ncbi
mouse monoclonal (ARFS 1A9/5)
  • western blot; human
In order to study the regulatory role of Arf1 in the Golgi and in hypersecretion in neuroendocrine cancer cells, Santa Cruz Biotechnology Arf1 antibody (Santa Cruz Biotechnology, ARFS 1A9/5) was used in western blot on human samples . J Cell Mol Med (2015) ncbi
mouse monoclonal (ARFS 1A9/5)
  • western blot; human; 1:1000; fig s14a
  • immunocytochemistry; mouse; fig s15a
Santa Cruz Biotechnology Arf1 antibody (Santa, sc-53168) was used in western blot on human samples at 1:1000 (fig s14a) and in immunocytochemistry on mouse samples (fig s15a). Science (2015) ncbi
Abcam
rabbit monoclonal (EPR443)
  • western blot; human; 1:1000; loading ...; fig 1a
Abcam Arf1 antibody (Abcam, ab108347) was used in western blot on human samples at 1:1000 (fig 1a). J Cell Biol (2017) ncbi
rabbit monoclonal (EPR443)
  • immunocytochemistry; human; fig 2
In order to report that PKD2 controls secretion of matrix metalloproteinase cargo, Abcam Arf1 antibody (Abcam, ab108347) was used in immunocytochemistry on human samples (fig 2). J Biol Chem (2016) ncbi
Novus Biologicals
mouse monoclonal (1D9)
  • western blot; human; 1:500; loading ...; fig s3a
In order to study the role of TRAPPC13 in autophagy and the cellular response to Golgi stress, Novus Biologicals Arf1 antibody (Novus Biologicals, NB300-505) was used in western blot on human samples at 1:500 (fig s3a). J Cell Sci (2017) ncbi
Invitrogen
mouse monoclonal (1D9)
  • immunocytochemistry; human; fig 9
  • western blot; human; fig 3
In order to report that the BAR domain of ASAP1 is a protein binding site for FIP3, Invitrogen Arf1 antibody (Affinity BioReagents, 1D9) was used in immunocytochemistry on human samples (fig 9) and in western blot on human samples (fig 3). Mol Biol Cell (2008) ncbi
mouse monoclonal (1D9)
  • immunocytochemistry; human; fig 5
  • western blot; human; fig 8
In order to describe a novel family of ubiquitous coat proteins, Invitrogen Arf1 antibody (Affinity Bioreagents, 1D9) was used in immunocytochemistry on human samples (fig 5) and in western blot on human samples (fig 8). J Cell Biol (2000) ncbi
Articles Reviewed
  1. Miyamoto Y, Torii T, Tago K, Tanoue A, Takashima S, Yamauchi J. BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by Schwann cells in mice. Sci Adv. 2018;4:eaar4471 pubmed publisher
  2. Chakraborti S, Sarkar J, Chowdhury A, Chakraborti T. Role of ADP ribosylation factor6- Cytohesin1-PhospholipaseD signaling axis in U46619 induced activation of NADPH oxidase in pulmonary artery smooth muscle cell membrane. Arch Biochem Biophys. 2017;633:1-14 pubmed publisher
  3. Ramírez Peinado S, Ignashkova T, van Raam B, Baumann J, Sennott E, Gendarme M, et al. TRAPPC13 modulates autophagy and the response to Golgi stress. J Cell Sci. 2017;130:2251-2265 pubmed publisher
  4. Yoo S, Latifkar A, Cerione R, Antonyak M. Cool-associated Tyrosine-phosphorylated Protein 1 Is Required for the Anchorage-independent Growth of Cervical Carcinoma Cells by Binding Paxillin and Promoting AKT Activation. J Biol Chem. 2017;292:3947-3957 pubmed publisher
  5. Rafiq N, Lieu Z, Jiang T, Yu C, Matsudaira P, Jones G, et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol. 2017;216:181-197 pubmed publisher
  6. Eiseler T, Wille C, Koehler C, Illing A, Seufferlein T. Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion. J Biol Chem. 2016;291:462-77 pubmed publisher
  7. Münzberg C, Höhn K, Krndija D, Maaß U, Bartsch D, Slater E, et al. IGF-1 drives chromogranin A secretion via activation of Arf1 in human neuroendocrine tumour cells. J Cell Mol Med. 2015;19:948-59 pubmed publisher
  8. Jewell J, Kim Y, Russell R, Yu F, Park H, Plouffe S, et al. Metabolism. Differential regulation of mTORC1 by leucine and glutamine. Science. 2015;347:194-8 pubmed publisher
  9. Inoue H, Ha V, Prekeris R, Randazzo P. Arf GTPase-activating protein ASAP1 interacts with Rab11 effector FIP3 and regulates pericentrosomal localization of transferrin receptor-positive recycling endosome. Mol Biol Cell. 2008;19:4224-37 pubmed publisher
  10. Dell Angelica E, Puertollano R, Mullins C, Aguilar R, Vargas J, Hartnell L, et al. GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex. J Cell Biol. 2000;149:81-94 pubmed