This is a Validated Antibody Database (VAD) review about rat Anxa1, based on 17 published articles (read how Labome selects the articles), using Anxa1 antibody in all methods. It is aimed to help Labome visitors find the most suited Anxa1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Anxa1 synonym: Anx1; p35; annexin A1; Annexin 1 (p35) (Lipocortin 1); annexin I; annexin-1; calpactin II; calpactin-2; chromobindin-9; lipocortin 1; lipocortin I; phospholipase A2 inhibitory protein

Knockout validation
Invitrogen
rabbit polyclonal
  • immunocytochemistry knockout validation; human; 1:100; fig 2
  • western blot knockout validation; human; 1:10,000; fig 1
In order to analyze the contribution to pancreatic cancer cell phenotype, behaviour and metastatic potential independently of formyl peptide receptor pathway by annexin A1, Invitrogen Anxa1 antibody (Invitrogen, 71-3400) was used in immunocytochemistry knockout validation on human samples at 1:100 (fig 2) and in western blot knockout validation on human samples at 1:10,000 (fig 1). Sci Rep (2016) ncbi
Invitrogen
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; fig s3a
In order to examine the role of the proresolving protein annexin A1 in healing after wire injury, Invitrogen Anxa1 antibody (Thermo Scientific, 71-3400) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig s3a). Arterioscler Thromb Vasc Biol (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...
In order to analyze the context specificity of signaling networks within a causal conceptual framework using reverse-phase protein array time-course assays and network analysis approaches, Invitrogen Anxa1 antibody (Invitrogen, 71-3400) was used in western blot on human samples . Cell Syst (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:10,000; loading ...; fig 2
In order to elucidate the mechanisms by which Annexin A1 governs metastatic behavior in prostate cancer cells exposed to hypoxia, Invitrogen Anxa1 antibody (Invitrogen, 71- 3400) was used in western blot on human samples at 1:10,000 (fig 2). Cell Adh Migr (2017) ncbi
rabbit polyclonal
  • immunocytochemistry knockout validation; human; 1:100; fig 2
  • western blot knockout validation; human; 1:10,000; fig 1
In order to analyze the contribution to pancreatic cancer cell phenotype, behaviour and metastatic potential independently of formyl peptide receptor pathway by annexin A1, Invitrogen Anxa1 antibody (Invitrogen, 71-3400) was used in immunocytochemistry knockout validation on human samples at 1:100 (fig 2) and in western blot knockout validation on human samples at 1:10,000 (fig 1). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse
  • western blot; mouse; loading ...; fig 2a
In order to characterize the P2X7R-secretome in M1 and M2 macrophages., Invitrogen Anxa1 antibody (Life Technology, 71-3400) was used in immunocytochemistry on mouse samples and in western blot on mouse samples (fig 2a). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 2g
In order to study the results of proteomic screening that identified calreticulin as a miR-27a direct target that represses MHC clas 1 cell surface exposure in colorectal cancer, Invitrogen Anxa1 antibody (Thermo Fisher, 71-3400) was used in western blot on human samples (fig 2g). Cell Death Dis (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 3
  • western blot; human; 1:1000; fig 3
In order to investigate the impact of PUFAs membrane incorporation on ABCA1-mediated cholesterol efflux, Invitrogen Anxa1 antibody (Invitrogen, 71-C3400) was used in western blot on mouse samples at 1:1000 (fig 3) and in western blot on human samples at 1:1000 (fig 3). Biochim Biophys Acta (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:150
In order to identify dysregulated genes in classical galactosaemia, Invitrogen Anxa1 antibody (Biosciences, 71-3400) was used in immunocytochemistry on human samples at 1:150. Mol Genet Metab (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 6
In order to discuss the clinical applications of using microRNA biomarkers in head and neck squamous cell carcinomas, Invitrogen Anxa1 antibody (Zymed Laboratories, 713400) was used in western blot on human samples at 1:1000 (fig 6). BMC Cancer (2013) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 4
In order to determine the functional role of PAX2 in ovarian cancer, Invitrogen Anxa1 antibody (invitrogen, 71-3400) was used in western blot on human samples at 1:1000 (fig 4). Int J Mol Sci (2013) ncbi
rabbit polyclonal
In order to compare expression profiles of intestinal polyps derived humans and mice, Invitrogen Anxa1 antibody (Zymed, 71-3400) was used . Am J Pathol (2008) ncbi
rabbit polyclonal
In order to study phagocytosis of Anx-A1 knockout macrophages, Invitrogen Anxa1 antibody (ZYMED, 71-3400) was used . Br J Pharmacol (2006) ncbi
Abcam
rabbit monoclonal (EPR19342)
  • western blot; human; loading ...; fig 5b, 5k
Abcam Anxa1 antibody (Abcam, ab214486) was used in western blot on human samples (fig 5b, 5k). Cell (2019) ncbi
Proteintech Group
rabbit polyclonal
  • immunohistochemistry; human; 1:100
  • western blot; human; 1:400
Proteintech Group Anxa1 antibody (ProteinTech Group, 55018-1-AP) was used in immunohistochemistry on human samples at 1:100 and in western blot on human samples at 1:400. Am J Transl Res (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:100
Proteintech Group Anxa1 antibody (ProteinTech Group, 55018-1-AP) was used in immunohistochemistry on human samples at 1:100. Expert Rev Mol Diagn (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (EH17a)
  • immunocytochemistry; human; fig 1
Santa Cruz Biotechnology Anxa1 antibody (Santa Cruz Biotechnology, sc-12740) was used in immunocytochemistry on human samples (fig 1). J Proteome Res (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 6b
Cell Signaling Technology Anxa1 antibody (Cell Signaling Technology, 3299) was used in immunoprecipitation on human samples (fig 6b). Front Mol Neurosci (2018) ncbi
Articles Reviewed
  1. Jeppesen D, Fenix A, Franklin J, Higginbotham J, Zhang Q, Zimmerman L, et al. Reassessment of Exosome Composition. Cell. 2019;177:428-445.e18 pubmed publisher
  2. Liu L, An D, Xu J, Shao B, Li X, Shi J. Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia. Front Mol Neurosci. 2018;11:76 pubmed publisher
  3. de Jong R, Paulin N, Lemnitzer P, Viola J, Winter C, Ferraro B, et al. Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. Arterioscler Thromb Vasc Biol. 2017;37:312-315 pubmed publisher
  4. Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
  5. Bizzarro V, Belvedere R, Migliaro V, Romano E, Parente L, Petrella A. Hypoxia regulates ANXA1 expression to support prostate cancer cell invasion and aggressiveness. Cell Adh Migr. 2017;11:247-260 pubmed publisher
  6. Belvedere R, Bizzarro V, Forte G, Dal Piaz F, Parente L, Petrella A. Annexin A1 contributes to pancreatic cancer cell phenotype, behaviour and metastatic potential independently of Formyl Peptide Receptor pathway. Sci Rep. 2016;6:29660 pubmed publisher
  7. de Torre Minguela C, Barberà Cremades M, Gómez A, Martín Sánchez F, Pelegrín P. Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process. Sci Rep. 2016;6:22586 pubmed publisher
  8. Colangelo T, Polcaro G, Ziccardi P, Pucci B, Muccillo L, Galgani M, et al. Proteomic screening identifies calreticulin as a miR-27a direct target repressing MHC class I cell surface exposure in colorectal cancer. Cell Death Dis. 2016;7:e2120 pubmed publisher
  9. Fournier N, Tardivel S, Benoist J, Vedie B, Rousseau Ralliard D, Nowak M, et al. Eicosapentaenoic acid membrane incorporation impairs ABCA1-dependent cholesterol efflux via a protein kinase A signaling pathway in primary human macrophages. Biochim Biophys Acta. 2016;1861:331-41 pubmed publisher
  10. Rong B, Zhao C, Liu H, Ming Z, Cai X, Gao W, et al. Elevated serum annexin A1 as potential diagnostic marker for lung cancer: a retrospective case-control study. Am J Transl Res. 2014;6:558-69 pubmed
  11. Biaoxue R, Shuanying Y, Wei L, Zongjuan M, Xiguang C, Qiuhong Z. Co-overexpression of Hsp90-β and annexin A1 with a significantly positive correlation contributes to the diagnosis of lung cancer. Expert Rev Mol Diagn. 2014;14:1067-79 pubmed publisher
  12. Coss K, Treacy E, Cotter E, Knerr I, Murray D, Shin Y, et al. Systemic gene dysregulation in classical Galactosaemia: Is there a central mechanism?. Mol Genet Metab. 2014;113:177-87 pubmed publisher
  13. Cypryk W, Ohman T, Eskelinen E, Matikainen S, Nyman T. Quantitative proteomics of extracellular vesicles released from human monocyte-derived macrophages upon ?-glucan stimulation. J Proteome Res. 2014;13:2468-77 pubmed publisher
  14. Severino P, Bruggemann H, Andreghetto F, Camps C, Klingbeil M, de Pereira W, et al. MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a and microRNA-10b dysregulation implicated in cell proliferation. BMC Cancer. 2013;13:533 pubmed publisher
  15. Song H, Kwan S, Izaguirre D, Zu Z, Tsang Y, Tung C, et al. PAX2 Expression in Ovarian Cancer. Int J Mol Sci. 2013;14:6090-105 pubmed publisher
  16. Gaspar C, Cardoso J, Franken P, Molenaar L, Morreau H, Möslein G, et al. Cross-species comparison of human and mouse intestinal polyps reveals conserved mechanisms in adenomatous polyposis coli (APC)-driven tumorigenesis. Am J Pathol. 2008;172:1363-80 pubmed publisher
  17. Yona S, Heinsbroek S, Peiser L, Gordon S, Perretti M, Flower R. Impaired phagocytic mechanism in annexin 1 null macrophages. Br J Pharmacol. 2006;148:469-77 pubmed