This is a Validated Antibody Database (VAD) review about rat Ano1, based on 19 published articles (read how Labome selects the articles), using Ano1 antibody in all methods. It is aimed to help Labome visitors find the most suited Ano1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ano1 synonym: Tmem16a; anoctamin-1; anoctamin 1, calcium activated chloride channel; transmembrane protein 16A

Knockout validation
Abcam
rabbit polyclonal
  • western blot knockout validation; mouse; 1:500; fig s11c
Abcam Ano1 antibody (abcam, ab53212) was used in western blot knockout validation on mouse samples at 1:500 (fig s11c). Nat Commun (2016) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig 5j
In order to investigate the role of anoctamin-2 in the spike-frequency adaptation of thalamocortical neurons, Abcam Ano1 antibody (Abcam, ab53212) was used in western blot on mouse samples at 1:500 (fig 5j). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; 1:500; fig s11c
Abcam Ano1 antibody (abcam, ab53212) was used in western blot knockout validation on mouse samples at 1:500 (fig s11c). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; tbl 1
  • immunohistochemistry - paraffin section; mouse; 1:200; tbl 1
Abcam Ano1 antibody (Abcam, ab53212) was used in immunohistochemistry - paraffin section on human samples at 1:200 (tbl 1) and in immunohistochemistry - paraffin section on mouse samples at 1:200 (tbl 1). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 7
Abcam Ano1 antibody (Abcam, 53213) was used in western blot on mouse samples (fig 7). Skelet Muscle (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 1c
In order to test if LRIG1 contributes to the ontogeny of interstitial cells of Cajal in the small and large intestines, Abcam Ano1 antibody (Abcam, ab53212) was used in immunohistochemistry on mouse samples at 1:100 (fig 1c). Gastroenterology (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:100; fig  4
In order to study duodenal adenomas in leucine-rich repeats and immunoglobulin-like domains 1 -/- mice, Abcam Ano1 antibody (Abcam, ab53212) was used in western blot on mouse samples at 1:100 (fig  4). Am J Pathol (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1600; fig s5
In order to study the human breast for transcriptional and epigenetic determinants, Abcam Ano1 antibody (Abcam, ab53212) was used in immunohistochemistry - paraffin section on human samples at 1:1600 (fig s5). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100
In order to evaluate diagnostic markers for lung cancer with bronchial brushing specimens, Abcam Ano1 antibody (Abcam, ab53212) was used in immunocytochemistry on human samples at 1:100. Cancer Cytopathol (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100
Abcam Ano1 antibody (Abcam, ab53212) was used in immunohistochemistry on mouse samples at 1:100. Neurogastroenterol Motil (2014) ncbi
rabbit polyclonal
  • immunoprecipitation; human; 4 ug
  • western blot; human; 1:200
Abcam Ano1 antibody (Abcam, ab53212) was used in immunoprecipitation on human samples at 4 ug and in western blot on human samples at 1:200. Br J Pharmacol (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:50
Abcam Ano1 antibody (Abcam, ab53212) was used in immunohistochemistry - frozen section on rat samples at 1:50. Brain Res Bull (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:500
Abcam Ano1 antibody (Abcam, ab53213) was used in western blot on mouse samples at 1:500. Br J Pharmacol (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:50
Abcam Ano1 antibody (Abcam, ab53212) was used in immunohistochemistry - frozen section on mouse samples at 1:50. Dev Neurobiol (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:50; fig 3
Abcam Ano1 antibody (Abcam, ab53212) was used in western blot on mouse samples at 1:50 (fig 3). Pflugers Arch (2013) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:1000; fig 4
Abcam Ano1 antibody (Abcam, Ab53212) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:1000 (fig 4). Biochem J (2013) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:500
Abcam Ano1 antibody (Abcam, ab53212) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:500. Am J Physiol Cell Physiol (2013) ncbi
Invitrogen
rabbit monoclonal (SP31)
  • immunohistochemistry; human; fig s1
In order to elucidate differential expression of TMEM16A/ANO1 in HPV-positive and HPV-negative head and neck squamous cell carcinoma through promoter methylation, Invitrogen Ano1 antibody (ThermoFisher, SP31) was used in immunohistochemistry on human samples (fig s1). Sci Rep (2015) ncbi
rabbit monoclonal (SP31)
  • immunohistochemistry; human; 1:50
Invitrogen Ano1 antibody (Thermo Scientific, SP31) was used in immunohistochemistry on human samples at 1:50. Acta Cytol (2015) ncbi
rabbit monoclonal (SP31)
  • immunohistochemistry; human; 1:100; tbl 1
In order to report on a case of plexiform fibromyxoma of the stomach, Invitrogen Ano1 antibody (Thermo, SP31) was used in immunohistochemistry on human samples at 1:100 (tbl 1). Int J Surg Pathol (2014) ncbi
Articles Reviewed
  1. Ha G, Lee J, Kwak H, Song K, Kwon J, Jung S, et al. The Ca2+-activated chloride channel anoctamin-2 mediates spike-frequency adaptation and regulates sensory transmission in thalamocortical neurons. Nat Commun. 2016;7:13791 pubmed publisher
  2. Zhang C, Wang P, Liu D, Chen C, Zhao W, Chen X, et al. The molecular basis of the genesis of basal tone in internal anal sphincter. Nat Commun. 2016;7:11358 pubmed publisher
  3. Brennan S, Wilkinson W, Tseng H, Finney B, Monk B, Dibble H, et al. The extracellular calcium-sensing receptor regulates human fetal lung development via CFTR. Sci Rep. 2016;6:21975 pubmed publisher
  4. Xu J, El Refaey M, Xu L, Zhao L, Gao Y, Floyd K, et al. Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy. Skelet Muscle. 2015;5:43 pubmed publisher
  5. Dixit R, Kemp C, Kulich S, Seethala R, Chiosea S, Ling S, et al. TMEM16A/ANO1 is differentially expressed in HPV-negative versus HPV-positive head and neck squamous cell carcinoma through promoter methylation. Sci Rep. 2015;5:16657 pubmed publisher
  6. Kondo J, Powell A, Wang Y, Musser M, Southard Smith E, Franklin J, et al. LRIG1 Regulates Ontogeny of Smooth Muscle-Derived Subsets of Interstitial Cells of Cajal in Mice. Gastroenterology. 2015;149:407-19.e8 pubmed publisher
  7. Wang Y, Shi C, Lu Y, Poulin E, Franklin J, Coffey R. Loss of Lrig1 leads to expansion of Brunner glands followed by duodenal adenomas with gastric metaplasia. Am J Pathol. 2015;185:1123-34 pubmed publisher
  8. Gascard P, Bilenky M, Sigaroudinia M, Zhao J, Li L, Carles A, et al. Epigenetic and transcriptional determinants of the human breast. Nat Commun. 2015;6:6351 pubmed publisher
  9. Kakkar A, Mathur S, Jain D, Iyer V, Nalwa A, Sharma M. Utility of DOG1 Immunomarker in Fine Needle Aspirates of Gastrointestinal Stromal Tumor. Acta Cytol. 2015;59:61-7 pubmed publisher
  10. Liu Y, Jiang Y, Wang B, Hao J, Shang L, Zhang T, et al. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer Cytopathol. 2014;122:833-41 pubmed publisher
  11. Wang X, Chen J, Li K, Zhu Y, Wright G, Huizinga J. Discrepancies between c-Kit positive and Ano1 positive ICC-SMP in the W/Wv and wild-type mouse colon; relationships with motor patterns and calcium transients. Neurogastroenterol Motil. 2014;26:1298-310 pubmed publisher
  12. Pritchard H, Leblanc N, Albert A, Greenwood I. Inhibitory role of phosphatidylinositol 4,5-bisphosphate on TMEM16A-encoded calcium-activated chloride channels in rat pulmonary artery. Br J Pharmacol. 2014;171:4311-21 pubmed publisher
  13. Kanazawa T, Matsumoto S. Expression of transient receptor potential vanilloid 1 and anoctamin 1 in rat trigeminal ganglion neurons innervating the tongue. Brain Res Bull. 2014;106:17-20 pubmed publisher
  14. Wu M, Lou J, Song B, Gong Y, Li Y, Yu C, et al. Hypoxia augments the calcium-activated chloride current carried by anoctamin-1 in cardiac vascular endothelial cells of neonatal mice. Br J Pharmacol. 2014;171:3680-92 pubmed publisher
  15. Maurya D, Menini A. Developmental expression of the calcium-activated chloride channels TMEM16A and TMEM16B in the mouse olfactory epithelium. Dev Neurobiol. 2014;74:657-75 pubmed publisher
  16. Jacobsen K, Zeeberg K, Sauter D, Poulsen K, Hoffmann E, Schwab A. The role of TMEM16A (ANO1) and TMEM16F (ANO6) in cell migration. Pflugers Arch. 2013;465:1753-62 pubmed publisher
  17. Lee P, Yau D, Lau P, Chan J. Plexiform fibromyxoma (plexiform angiomyxoid myofibroblastic tumor) of stomach: an unusual presentation as a fistulating abscess. Int J Surg Pathol. 2014;22:286-90 pubmed publisher
  18. Scudieri P, Sondo E, Caci E, Ravazzolo R, Galietta L. TMEM16A-TMEM16B chimaeras to investigate the structure-function relationship of calcium-activated chloride channels. Biochem J. 2013;452:443-55 pubmed publisher
  19. Wang J, Haanes K, Novak I. Purinergic regulation of CFTR and Ca(2+)-activated Cl(-) channels and K(+) channels in human pancreatic duct epithelium. Am J Physiol Cell Physiol. 2013;304:C673-84 pubmed publisher