This is a Validated Antibody Database (VAD) review about rat Adam10, based on 17 published articles (read how Labome selects the articles), using Adam10 antibody in all methods. It is aimed to help Labome visitors find the most suited Adam10 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Adam10 synonym: MADM; disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; RGD1566370; a disintegrin and metallopeptidase domain 10; a disintegrin and metalloprotease domain 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease

Knockout validation
Abcam
rabbit monoclonal (EPR5622)
  • western blot knockout validation; mouse; fig 2
Abcam Adam10 antibody (Abcam/Epitomics, EPR5622) was used in western blot knockout validation on mouse samples (fig 2). elife (2016) ncbi
Abcam
rabbit monoclonal (EPR5622)
  • western blot; mouse; fig 2a
Abcam Adam10 antibody (Abcam, EPR5622) was used in western blot on mouse samples (fig 2a). Cell Mol Life Sci (2018) ncbi
rabbit monoclonal (EPR5622)
  • western blot; mouse; 1:500; loading ...; fig 4a
Abcam Adam10 antibody (Abcam, ab124695) was used in western blot on mouse samples at 1:500 (fig 4a). Front Pharmacol (2017) ncbi
rabbit monoclonal (EPR5622)
  • western blot knockout validation; mouse; fig 2
Abcam Adam10 antibody (Abcam/Epitomics, EPR5622) was used in western blot knockout validation on mouse samples (fig 2). elife (2016) ncbi
rabbit monoclonal (EPR5622)
  • immunoprecipitation; hamsters; fig 2
  • western blot; hamsters; fig 2
In order to identify and characterize a multiprotease complex containing both alpha- and gamma-secretase, Abcam Adam10 antibody (Abcam, ab124695) was used in immunoprecipitation on hamsters samples (fig 2) and in western blot on hamsters samples (fig 2). J Cell Biol (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-3)
  • proximity ligation assay; pig; 1:100; fig 5
  • immunocytochemistry; pig; 1:100; fig 1
In order to characterize cell junction assembly in early porcine embryo development by ADAM10, Santa Cruz Biotechnology Adam10 antibody (Santa Cruz, sc-48400) was used in proximity ligation assay on pig samples at 1:100 (fig 5) and in immunocytochemistry on pig samples at 1:100 (fig 1). PLoS ONE (2016) ncbi
mouse monoclonal (A-3)
  • western blot; human; 1:500; loading ...; fig st3
In order to study the DNA damage response in senescent epithelial cells, Santa Cruz Biotechnology Adam10 antibody (Santa Cruz, sc-48400) was used in western blot on human samples at 1:500 (fig st3). Nat Commun (2016) ncbi
mouse monoclonal (B-3)
  • immunocytochemistry; mouse; 1:500
Santa Cruz Biotechnology Adam10 antibody (Santa, sc-28358) was used in immunocytochemistry on mouse samples at 1:500. Proc Natl Acad Sci U S A (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; mouse; fig 3d
In order to show that ALOX5 upregulation results in homocysteine-dependent worsening of the Alzheimer's disease phenotype, EMD Millipore Adam10 antibody (Millipore, AB19026) was used in western blot on mouse samples (fig 3d). Sci Rep (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; hamsters; fig 2
  • immunocytochemistry; hamsters; fig 6
  • western blot; hamsters; fig 2
In order to identify and characterize a multiprotease complex containing both alpha- and gamma-secretase, EMD Millipore Adam10 antibody (Millipore, ab19026) was used in immunoprecipitation on hamsters samples (fig 2), in immunocytochemistry on hamsters samples (fig 6) and in western blot on hamsters samples (fig 2). J Cell Biol (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:500
EMD Millipore Adam10 antibody (Merck Millipore, AB-19026) was used in western blot on mouse samples at 1:500. Oxid Med Cell Longev (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 3
EMD Millipore Adam10 antibody (Millipore, AB19026) was used in western blot on human samples at 1:1000 (fig 3). J Pineal Res (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
EMD Millipore Adam10 antibody (Millipore, AB19026) was used in western blot on mouse samples at 1:1000. Proc Natl Acad Sci U S A (2015) ncbi
rabbit polyclonal
  • immunoprecipitation; human
  • western blot; human
In order to study cadherin-11 extracellular domain fragments in rheumatoid arthritis, EMD Millipore Adam10 antibody (EMD Millipore, AB19026) was used in immunoprecipitation on human samples and in western blot on human samples . Arthritis Res Ther (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:400
EMD Millipore Adam10 antibody (Millipore, AB19026) was used in western blot on human samples at 1:400. J Pineal Res (2015) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore Adam10 antibody (Millipore, AB19026) was used in western blot on human samples . J Biol Chem (2014) ncbi
rabbit polyclonal
  • western blot; human
In order to study the release of soluble giant cadherin Fat1 from pancreatic cancer cells, EMD Millipore Adam10 antibody (Millipore, AB19026) was used in western blot on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; chicken
  • western blot; chicken
EMD Millipore Adam10 antibody (Chemicon, AB19026) was used in immunohistochemistry - frozen section on chicken samples and in western blot on chicken samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
EMD Millipore Adam10 antibody (Millipore, AB19026) was used in western blot on human samples at 1:1000. J Biol Chem (2013) ncbi
rabbit polyclonal
  • western blot; human; fig 6
  • western blot; mouse
EMD Millipore Adam10 antibody (Millipore, AB19026) was used in western blot on human samples (fig 6) and in western blot on mouse samples . J Lipid Res (2013) ncbi
Articles Reviewed
  1. Seipold L, Altmeppen H, Koudelka T, Tholey A, Kaspárek P, Sedlacek R, et al. In vivo regulation of the A disintegrin and metalloproteinase 10 (ADAM10) by the tetraspanin 15. Cell Mol Life Sci. 2018;75:3251-3267 pubmed publisher
  2. Li J, Barrero C, Merali S, Pratico D. Five lipoxygenase hypomethylation mediates the homocysteine effect on Alzheimer's phenotype. Sci Rep. 2017;7:46002 pubmed publisher
  3. Yan L, Deng Y, Gao J, Liu Y, Li F, Shi J, et al. Icariside II Effectively Reduces Spatial Learning and Memory Impairments in Alzheimer's Disease Model Mice Targeting Beta-Amyloid Production. Front Pharmacol. 2017;8:106 pubmed publisher
  4. Kwon J, Jeong S, Choi I, Kim N. ADAM10 Is Involved in Cell Junction Assembly in Early Porcine Embryo Development. PLoS ONE. 2016;11:e0152921 pubmed publisher
  5. Nassour J, Martien S, Martin N, Deruy E, Tomellini E, Malaquin N, et al. Defective DNA single-strand break repair is responsible for senescence and neoplastic escape of epithelial cells. Nat Commun. 2016;7:10399 pubmed publisher
  6. Kuhn P, Colombo A, Schusser B, Dreymueller D, Wetzel S, Schepers U, et al. Systematic substrate identification indicates a central role for the metalloprotease ADAM10 in axon targeting and synapse function. elife. 2016;5: pubmed publisher
  7. Chen A, Kim S, Shepardson N, Patel S, Hong S, Selkoe D. Physical and functional interaction between the α- and γ-secretases: A new model of regulated intramembrane proteolysis. J Cell Biol. 2015;211:1157-76 pubmed publisher
  8. Schloesser A, Esatbeyoglu T, Piegholdt S, Dose J, Ikuta N, Okamoto H, et al. Dietary Tocotrienol/γ-Cyclodextrin Complex Increases Mitochondrial Membrane Potential and ATP Concentrations in the Brains of Aged Mice. Oxid Med Cell Longev. 2015;2015:789710 pubmed publisher
  9. Panmanee J, Nopparat C, Chavanich N, Shukla M, Mukda S, Song W, et al. Melatonin regulates the transcription of βAPP-cleaving secretases mediated through melatonin receptors in human neuroblastoma SH-SY5Y cells. J Pineal Res. 2015;59:308-20 pubmed publisher
  10. Corbett G, Gonzalez F, Pahan K. Activation of peroxisome proliferator-activated receptor α stimulates ADAM10-mediated proteolysis of APP. Proc Natl Acad Sci U S A. 2015;112:8445-50 pubmed publisher
  11. Noss E, Watts G, Zocco D, Keller T, Whitman M, Blobel C, et al. Evidence for cadherin-11 cleavage in the synovium and partial characterization of its mechanism. Arthritis Res Ther. 2015;17:126 pubmed publisher
  12. Shukla M, Htoo H, Wintachai P, Hernandez J, Dubois C, Postina R, et al. Melatonin stimulates the nonamyloidogenic processing of βAPP through the positive transcriptional regulation of ADAM10 and ADAM17. J Pineal Res. 2015;58:151-65 pubmed publisher
  13. Herzog C, Haun R, Ludwig A, Shah S, Kaushal G. ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A. J Biol Chem. 2014;289:13308-22 pubmed publisher
  14. Wojtalewicz N, Sadeqzadeh E, Weiß J, Tehrani M, Klein Scory S, Hahn S, et al. A soluble form of the giant cadherin Fat1 is released from pancreatic cancer cells by ADAM10 mediated ectodomain shedding. PLoS ONE. 2014;9:e90461 pubmed publisher
  15. Yan X, Lin J, Talabattula V, Mußmann C, Yang F, Wree A, et al. ADAM10 negatively regulates neuronal differentiation during spinal cord development. PLoS ONE. 2014;9:e84617 pubmed publisher
  16. Woods N, Padmanabhan J. Inhibition of amyloid precursor protein processing enhances gemcitabine-mediated cytotoxicity in pancreatic cancer cells. J Biol Chem. 2013;288:30114-24 pubmed publisher
  17. Quade Lyssy P, Kanarek A, Baiersdörfer M, Postina R, Kojro E. Statins stimulate the production of a soluble form of the receptor for advanced glycation end products. J Lipid Res. 2013;54:3052-61 pubmed publisher