This is a Validated Antibody Database (VAD) review about rat Abhd5, based on 13 published articles (read how Labome selects the articles), using Abhd5 antibody in all methods. It is aimed to help Labome visitors find the most suited Abhd5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Abhd5 synonym: CGI-58; 1-acylglycerol-3-phosphate O-acyltransferase ABHD5; abhydrolase domain-containing protein 5; lipid droplet-binding protein CGI-58

Knockout validation
Abnova
mouse monoclonal (1F3)
  • western blot knockout validation; human; fig 1k
  • immunoprecipitation; human; fig 5b
  • immunocytochemistry; human; fig 5a
  • immunohistochemistry; human; loading ...; fig 8a
In order to propose that ABHD5 has a PNPLA2-independent function in regulating autophagy and tumorigenesis, Abnova Abhd5 antibody (Abnova, H00051099-M01) was used in western blot knockout validation on human samples (fig 1k), in immunoprecipitation on human samples (fig 5b), in immunocytochemistry on human samples (fig 5a) and in immunohistochemistry on human samples (fig 8a). Autophagy (2016) ncbi
Abnova
mouse monoclonal (1F3)
  • western blot; human; 1:250; loading ...; fig 1d
Abnova Abhd5 antibody (Abnova, H00051099-M01) was used in western blot on human samples at 1:250 (fig 1d). Int J Obes (Lond) (2017) ncbi
mouse monoclonal (1F3)
  • western blot knockout validation; human; fig 1k
  • immunoprecipitation; human; fig 5b
  • immunocytochemistry; human; fig 5a
  • immunohistochemistry; human; loading ...; fig 8a
In order to propose that ABHD5 has a PNPLA2-independent function in regulating autophagy and tumorigenesis, Abnova Abhd5 antibody (Abnova, H00051099-M01) was used in western blot knockout validation on human samples (fig 1k), in immunoprecipitation on human samples (fig 5b), in immunocytochemistry on human samples (fig 5a) and in immunohistochemistry on human samples (fig 8a). Autophagy (2016) ncbi
mouse monoclonal (1F3)
  • western blot; mouse; 1:1000; fig 1
Abnova Abhd5 antibody (Abnova, H00051099-M01) was used in western blot on mouse samples at 1:1000 (fig 1). Nat Commun (2016) ncbi
mouse monoclonal (1F3)
  • immunocytochemistry; human; 1:1000; fig s3
  • western blot; human; 1:1000; fig 2
Abnova Abhd5 antibody (Abnova, H00051099-M01) was used in immunocytochemistry on human samples at 1:1000 (fig s3) and in western blot on human samples at 1:1000 (fig 2). PLoS Pathog (2016) ncbi
mouse monoclonal (1F3)
  • immunoprecipitation; mouse; 5:700; loading ...; fig 1e
  • western blot; mouse; loading ...; fig 1e
Abnova Abhd5 antibody (Abnova, H00051099-M01) was used in immunoprecipitation on mouse samples at 5:700 (fig 1e) and in western blot on mouse samples (fig 1e). J Biol Chem (2015) ncbi
mouse monoclonal (1F3)
  • western blot; mouse; fig 1d
Abnova Abhd5 antibody (Abnova, H00051099-M01) was used in western blot on mouse samples (fig 1d). Int J Mol Sci (2015) ncbi
mouse monoclonal (1F3)
  • western blot; human
In order to study the role of skeletal muscle lipid handling in obese patients with type 2 diabetes, Abnova Abhd5 antibody (Abnova, H00051099-M01) was used in western blot on human samples . PLoS ONE (2015) ncbi
mouse monoclonal (1F3)
  • western blot; human; 1:800
Abnova Abhd5 antibody (Abnova, H00051099-M01) was used in western blot on human samples at 1:800. Diabetes (2014) ncbi
Novus Biologicals
rabbit polyclonal
  • western blot; rat
Novus Biologicals Abhd5 antibody (Novus Biologicals, NB110-41576) was used in western blot on rat samples . PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:1,000
Novus Biologicals Abhd5 antibody (Novus Biologicals, NB110-41576) was used in western blot on rat samples at 1:1,000. Lipids (2015) ncbi
rabbit polyclonal
  • western blot; mouse
  • western blot; rat
Novus Biologicals Abhd5 antibody (Novus Biologicals, NB110-41576) was used in western blot on mouse samples and in western blot on rat samples . Physiol Rep (2013) ncbi
rabbit polyclonal
  • western blot; mouse
Novus Biologicals Abhd5 antibody (NOVUS Biologicals, NB110-41576) was used in western blot on mouse samples . J Physiol (2013) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; rat
Sigma-Aldrich Abhd5 antibody (Sigma-Aldrich, AV42455) was used in western blot on rat samples . J Steroid Biochem Mol Biol (2015) ncbi
Articles Reviewed
  1. Hirschmugl B, Desoye G, Catalano P, Klymiuk I, Scharnagl H, Payr S, et al. Maternal obesity modulates intracellular lipid turnover in the human term placenta. Int J Obes (Lond). 2017;41:317-323 pubmed publisher
  2. Peng Y, Miao H, Wu S, Yang W, Zhang Y, Xie G, et al. ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2. Autophagy. 2016;12:2167-2182 pubmed
  3. Miao H, Ou J, Peng Y, Zhang X, Chen Y, Hao L, et al. Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM. Nat Commun. 2016;7:11716 pubmed publisher
  4. Vieyres G, Welsch K, Gerold G, Gentzsch J, Kahl S, Vondran F, et al. ABHD5/CGI-58, the Chanarin-Dorfman Syndrome Protein, Mobilises Lipid Stores for Hepatitis C Virus Production. PLoS Pathog. 2016;12:e1005568 pubmed publisher
  5. Hofer P, Boeszoermenyi A, Jaeger D, Feiler U, Arthanari H, Mayer N, et al. Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling. J Biol Chem. 2015;290:18438-53 pubmed publisher
  6. Das S, Stadelmeyer E, Schauer S, Schwarz A, Strohmaier H, Claudel T, et al. Micro RNA-124a regulates lipolysis via adipose triglyceride lipase and comparative gene identification 58. Int J Mol Sci. 2015;16:8555-68 pubmed publisher
  7. Turnbull P, Ramos S, Macpherson R, Roy B, Peters S. Characterization of lipolytic inhibitor G(0)/G(1) switch gene-2 protein (G0S2) expression in male Sprague-Dawley rat skeletal muscle compared to relative content of adipose triglyceride lipase (ATGL) and comparitive gene identification-58 (CGI-58). PLoS ONE. 2015;10:e0120136 pubmed publisher
  8. Bakke S, Feng Y, Nikolić N, Kase E, Moro C, Stensrud C, et al. Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects. PLoS ONE. 2015;10:e0119556 pubmed publisher
  9. Macpherson R, Castelli L, Miotto P, Frendo Cumbo S, Milburn A, Roy B, et al. A maternal high fat diet has long-lasting effects on skeletal muscle lipid and PLIN protein content in rat offspring at young adulthood. Lipids. 2015;50:205-17 pubmed publisher
  10. Stelmanska E, Szrok S, Swierczynski J. Progesterone-induced down-regulation of hormone sensitive lipase (Lipe) and up-regulation of G0/G1 switch 2 (G0s2) genes expression in inguinal adipose tissue of female rats is reflected by diminished rate of lipolysis. J Steroid Biochem Mol Biol. 2015;147:31-9 pubmed publisher
  11. Kurtz C, Peck B, Fannin E, Beysen C, Miao J, Landstreet S, et al. MicroRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes. Diabetes. 2014;63:3141-8 pubmed publisher
  12. Macpherson R, Vandenboom R, Roy B, Peters S. Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation. Physiol Rep. 2013;1:e00084 pubmed publisher
  13. Alsted T, Ploug T, Prats C, Serup A, Høeg L, Schjerling P, et al. Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle. J Physiol. 2013;591:5141-55 pubmed publisher