This is a Validated Antibody Database (VAD) review about rabbit MYH4, based on 21 published articles (read how Labome selects the articles), using MYH4 antibody in all methods. It is aimed to help Labome visitors find the most suited MYH4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MYH4 synonym: MHC; MYH1; myosin-4; myHC-2b; myosin heavy chain 2X; myosin heavy chain 2b; myosin heavy chain 4; myosin heavy chain, skeletal muscle, juvenile

Invitrogen
mouse monoclonal (MF20)
  • flow cytometry; mouse; 1:100; loading ...; fig 3b
Invitrogen MYH4 antibody (eBioscience, 53-6503-82) was used in flow cytometry on mouse samples at 1:100 (fig 3b). Mol Med Rep (2018) ncbi
mouse monoclonal (MF20)
  • immunohistochemistry - frozen section; zebrafish ; 1:75; loading ...; fig s2c
In order to examine cardiac revascularization in zebrafish, Invitrogen MYH4 antibody (eBiosciences, MF20) was used in immunohistochemistry - frozen section on zebrafish samples at 1:75 (fig s2c). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (MF20)
  • flow cytometry; mouse
Invitrogen MYH4 antibody (Affymetrix eBioscience, 53-6503-82) was used in flow cytometry on mouse samples . Circ Cardiovasc Genet (2014) ncbi
mouse monoclonal (MF20)
  • immunocytochemistry; mouse; 1:200
In order to analyze the conversion increase of fibroblasts to induced cardiomyocytes through inhibition of TGFbeta signaling, Invitrogen MYH4 antibody (eBioscience, 53-6503-82) was used in immunocytochemistry on mouse samples at 1:200. PLoS ONE (2014) ncbi
mouse monoclonal (MF20)
  • immunocytochemistry; mouse; 1:200
In order to evaluate the method for measuring fibroblast reprogramming to cardiomyocytes, Invitrogen MYH4 antibody (eBioscience, 53-6503-82) was used in immunocytochemistry on mouse samples at 1:200. J Mol Cell Cardiol (2013) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (6H1)
  • western blot; mouse; loading ...; fig 3c
In order to study the role of PPAR beta in PGC-1 alpha metabolism and mitochondria integrity, Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in western blot on mouse samples (fig 3c). Cell Metab (2017) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1f
In order to characterize the importance of glycolysis transcriptional regulator Nur77 during muscle growth, Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry - frozen section on mouse samples (fig 1f). PLoS ONE (2017) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry; human; 1:25; loading ...
In order to study the effects of ageing, physical activity, pre-frailty on skeletal muscle phenotype and examine the mitochondrial and intramyocellular lipid content of men, Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry on human samples at 1:25. J Cachexia Sarcopenia Muscle (2017) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry; mouse; 1:2
In order to investigate the contribution of TEAD1 to muscle regeneration and pathology, Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry on mouse samples at 1:2. elife (2016) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry; human; loading ...; fig 1a
In order to discuss factors that contribute to the decrease mobility of patients with lower extremity peripheral artery disease, Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry on human samples (fig 1a). J Transl Med (2016) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry - frozen section; human; 1:25; loading ...; fig 4a
In order to clarify the role of denervation in modulating mitochondrial function in ageing muscle, Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry - frozen section on human samples at 1:25 (fig 4a). J Physiol (2016) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry - frozen section; rat; 1:25; fig 1
In order to discuss the age-related presence of denervated myofibers and accelerated muscle atrophy, Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry - frozen section on rat samples at 1:25 (fig 1). Skelet Muscle (2016) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry - frozen section; mouse; 1:5; fig 5
Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry - frozen section on mouse samples at 1:5 (fig 5). PLoS Genet (2016) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry - frozen section; human; 1:25; fig 1b
In order to investigate the effects of age on the resilience of slow type fibers, Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry - frozen section on human samples at 1:25 (fig 1b). Am J Physiol Cell Physiol (2016) ncbi
mouse monoclonal (10F5)
  • immunocytochemistry; mouse; 1:50; loading ...; fig 1d
  • western blot; mouse; 1:500; loading ...; fig 1b
Developmental Studies Hybridoma Bank MYH4 antibody (Hybridoma Bank, 10F5) was used in immunocytochemistry on mouse samples at 1:50 (fig 1d) and in western blot on mouse samples at 1:500 (fig 1b). Cell Signal (2016) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry; mouse; fig 1
Developmental Studies Hybridoma Bank MYH4 antibody (Developmental Studies Hybridoma Bank, 6H1) was used in immunohistochemistry on mouse samples (fig 1). J Biol Chem (2015) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry; human
Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry on human samples . Physiol Rep (2015) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry - frozen section; mouse; 1:600; fig 9
In order to study mice lacking ERK1/2 selectively in skeletal myofibers, Developmental Studies Hybridoma Bank MYH4 antibody (Developmental Studies Hybridoma Bank, 6H1) was used in immunohistochemistry - frozen section on mouse samples at 1:600 (fig 9). Mol Cell Biol (2015) ncbi
mouse monoclonal (10F5)
  • immunohistochemistry - frozen section; mouse; 1:600; fig 9
In order to study mice lacking ERK1/2 selectively in skeletal myofibers, Developmental Studies Hybridoma Bank MYH4 antibody (Developmental Studies Hybridoma Bank, 10F5) was used in immunohistochemistry - frozen section on mouse samples at 1:600 (fig 9). Mol Cell Biol (2015) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry - frozen section; mouse; fig 1
Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry - frozen section on mouse samples (fig 1). Mol Cell Biol (2015) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry; rat; 1:25
  • immunohistochemistry; human; 1:25
  • immunohistochemistry; mouse; 1:25
Developmental Studies Hybridoma Bank MYH4 antibody (Developmental Studies Hybridoma Bank, 6H1) was used in immunohistochemistry on rat samples at 1:25, in immunohistochemistry on human samples at 1:25 and in immunohistochemistry on mouse samples at 1:25. PLoS ONE (2014) ncbi
mouse monoclonal (6H1)
  • immunohistochemistry; human
Developmental Studies Hybridoma Bank MYH4 antibody (DSHB, 6H1) was used in immunohistochemistry on human samples . J Physiol (2014) ncbi
Articles Reviewed
  1. Tian J, Wang R, Hou Q, Li M, Chen L, Deng X, et al. Optimization and enrichment of induced cardiomyocytes derived from mouse fibroblasts by reprogramming with cardiac transcription factors. Mol Med Rep. 2018;17:3912-3920 pubmed publisher
  2. Koh J, Hancock C, Terada S, Higashida K, Holloszy J, Han D. PPARβ Is Essential for Maintaining Normal Levels of PGC-1α and Mitochondria and for the Increase in Muscle Mitochondria Induced by Exercise. Cell Metab. 2017;25:1176-1185.e5 pubmed publisher
  3. Cortez Toledo O, Schnair C, Sangngern P, Metzger D, Chao L. Nur77 deletion impairs muscle growth during developmental myogenesis and muscle regeneration in mice. PLoS ONE. 2017;12:e0171268 pubmed publisher
  4. St Jean Pelletier F, Pion C, Leduc Gaudet J, Sgarioto N, Zovilé I, Barbat Artigas S, et al. The impact of ageing, physical activity, and pre-frailty on skeletal muscle phenotype, mitochondrial content, and intramyocellular lipids in men. J Cachexia Sarcopenia Muscle. 2017;8:213-228 pubmed publisher
  5. Southard S, Kim J, Low S, Tsika R, Lepper C. Myofiber-specific TEAD1 overexpression drives satellite cell hyperplasia and counters pathological effects of dystrophin deficiency. elife. 2016;5: pubmed publisher
  6. White S, McDermott M, Sufit R, Kosmac K, Bugg A, Gonzalez Freire M, et al. Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study. J Transl Med. 2016;14:284 pubmed publisher
  7. Marín Juez R, Marass M, Gauvrit S, Rossi A, Lai S, Materna S, et al. Fast revascularization of the injured area is essential to support zebrafish heart regeneration. Proc Natl Acad Sci U S A. 2016;113:11237-11242 pubmed
  8. Spendiff S, Vuda M, Gouspillou G, Aare S, Pérez A, Morais J, et al. Denervation drives mitochondrial dysfunction in skeletal muscle of octogenarians. J Physiol. 2016;594:7361-7379 pubmed publisher
  9. Aare S, Spendiff S, Vuda M, Elkrief D, Pérez A, Wu Q, et al. Failed reinnervation in aging skeletal muscle. Skelet Muscle. 2016;6:29 pubmed publisher
  10. Riaz M, Raz Y, van Putten M, Paniagua Soriano G, Krom Y, Florea B, et al. PABPN1-Dependent mRNA Processing Induces Muscle Wasting. PLoS Genet. 2016;12:e1006031 pubmed publisher
  11. Power G, Minozzo F, Spendiff S, Filion M, Konokhova Y, Purves Smith M, et al. Reduction in single muscle fiber rate of force development with aging is not attenuated in world class older masters athletes. Am J Physiol Cell Physiol. 2016;310:C318-27 pubmed publisher
  12. Zhang Y, Li W, Zhu M, Li Y, Xu Z, Zuo B. FHL3 differentially regulates the expression of MyHC isoforms through interactions with MyoD and pCREB. Cell Signal. 2016;28:60-73 pubmed publisher
  13. Ebert S, Dyle M, Bullard S, Dierdorff J, Murry D, Fox D, et al. Identification and Small Molecule Inhibition of an Activating Transcription Factor 4 (ATF4)-dependent Pathway to Age-related Skeletal Muscle Weakness and Atrophy. J Biol Chem. 2015;290:25497-511 pubmed publisher
  14. Walton R, Finlin B, Mula J, Long D, Zhu B, Fry C, et al. Insulin-resistant subjects have normal angiogenic response to aerobic exercise training in skeletal muscle, but not in adipose tissue. Physiol Rep. 2015;3: pubmed publisher
  15. Seaberg B, Henslee G, Wang S, Paez Colasante X, Landreth G, Rimer M. Muscle-derived extracellular signal-regulated kinases 1 and 2 are required for the maintenance of adult myofibers and their neuromuscular junctions. Mol Cell Biol. 2015;35:1238-53 pubmed publisher
  16. Tontonoz P, Cortez Toledo O, Wroblewski K, Hong C, Lim L, Carranza R, et al. The orphan nuclear receptor Nur77 is a determinant of myofiber size and muscle mass in mice. Mol Cell Biol. 2015;35:1125-38 pubmed publisher
  17. Gouspillou G, Sgarioto N, Norris B, Barbat Artigas S, Aubertin Leheudre M, Morais J, et al. The relationship between muscle fiber type-specific PGC-1α content and mitochondrial content varies between rodent models and humans. PLoS ONE. 2014;9:e103044 pubmed publisher
  18. Martinez Fernandez A, Nelson T, Reyes S, Alekseev A, Secreto F, Perez Terzic C, et al. iPS cell-derived cardiogenicity is hindered by sustained integration of reprogramming transgenes. Circ Cardiovasc Genet. 2014;7:667-76 pubmed publisher
  19. Fry C, Noehren B, Mula J, Ubele M, Westgate P, Kern P, et al. Fibre type-specific satellite cell response to aerobic training in sedentary adults. J Physiol. 2014;592:2625-35 pubmed publisher
  20. Ifkovits J, Addis R, Epstein J, Gearhart J. Inhibition of TGF? signaling increases direct conversion of fibroblasts to induced cardiomyocytes. PLoS ONE. 2014;9:e89678 pubmed publisher
  21. Addis R, Ifkovits J, Pinto F, Kellam L, Esteso P, Rentschler S, et al. Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. J Mol Cell Cardiol. 2013;60:97-106 pubmed publisher