This is a Validated Antibody Database (VAD) review about rabbit MAPT, based on 9 published articles (read how Labome selects the articles), using MAPT antibody in all methods. It is aimed to help Labome visitors find the most suited MAPT antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MAPT synonym: microtubule-associated protein tau

Knockout validation
Invitrogen
mouse monoclonal (T46)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1b
In order to show that the tau family members MAP2 and MAP4 are subject to reversible acetylation, Invitrogen MAPT antibody (Thermofisher, T46) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1b). PLoS ONE (2016) ncbi
Invitrogen
mouse monoclonal (T46)
  • immunoprecipitation; human
  • western blot; human; 1:1000; fig 1b
In order to study the mechanisms of pathogenesis via tau acetylation in Alzheimer's disease, Invitrogen MAPT antibody (Thermo Fisher, 13-6400) was used in immunoprecipitation on human samples and in western blot on human samples at 1:1000 (fig 1b). Sci Rep (2017) ncbi
mouse monoclonal (T46)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1b
In order to show that the tau family members MAP2 and MAP4 are subject to reversible acetylation, Invitrogen MAPT antibody (Thermofisher, T46) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1b). PLoS ONE (2016) ncbi
mouse monoclonal (T46)
  • immunocytochemistry; rat; 1:100; loading ...; fig 4b
In order to report the effects of okadaic acid-induced tau hyperphosphorylation on secreted and cellular levels of brain-derived neurotrophic factor in primary cortical neurons, Invitrogen MAPT antibody (Thermofisher, 13-6400) was used in immunocytochemistry on rat samples at 1:100 (fig 4b). J Chem Neuroanat (2017) ncbi
mouse monoclonal (T46)
  • immunocytochemistry; human; 1:200; fig 2c
  • western blot; human; 1:1000; fig 2e
In order to discuss the contribution of leucine-rich repeat kinase 2 mutations to Parkinson's disease, Invitrogen MAPT antibody (Invitrogen, 136400) was used in immunocytochemistry on human samples at 1:200 (fig 2c) and in western blot on human samples at 1:1000 (fig 2e). J Neuroinflammation (2016) ncbi
mouse monoclonal (T46)
  • western blot; mouse; 1:1000; fig 7
In order to assess rescue of Alzheimer's disease in the mouse by improved proteostasis in the secretory pathway, Invitrogen MAPT antibody (Invitrogen, T46) was used in western blot on mouse samples at 1:1000 (fig 7). Brain (2016) ncbi
mouse monoclonal (T46)
  • immunohistochemistry; human; 1:500; fig 6
  • western blot; human; fig 2
In order to assess using the biochemical properties of tau to classify tauopathies, Invitrogen MAPT antibody (Thermo Scientific, T46) was used in immunohistochemistry on human samples at 1:500 (fig 6) and in western blot on human samples (fig 2). Acta Neuropathol (2016) ncbi
mouse monoclonal (T46)
  • western blot; human
In order to identify small-molecules that inhibit the interaction between CDK5 and p25, Invitrogen MAPT antibody (Invitrogen, 13-6400) was used in western blot on human samples . Chem Biol (2015) ncbi
mouse monoclonal (T46)
  • western blot; human; 1:1000
In order to report a case of glial mixed tau and TDP-43 proteinopathies in a Japanese patient, Invitrogen MAPT antibody (Zymed, T46) was used in western blot on human samples at 1:1000. Brain Pathol (2016) ncbi
mouse monoclonal (T46)
  • western blot; human; fig 1
In order to characterize two mouse models overexpressing different human tau protein constructs, Invitrogen MAPT antibody (Invitrogen, 13-6400) was used in western blot on human samples (fig 1). Cell Mol Life Sci (2015) ncbi
Articles Reviewed
  1. Trzeciakiewicz H, Tseng J, Wander C, Madden V, Tripathy A, Yuan C, et al. A Dual Pathogenic Mechanism Links Tau Acetylation to Sporadic Tauopathy. Sci Rep. 2017;7:44102 pubmed publisher
  2. Hwang A, Trzeciakiewicz H, Friedmann D, Yuan C, Marmorstein R, Lee V, et al. Conserved Lysine Acetylation within the Microtubule-Binding Domain Regulates MAP2/Tau Family Members. PLoS ONE. 2016;11:e0168913 pubmed publisher
  3. Atasoy Ä, Dursun E, Gezen Ak D, Metin ArmaÄŸan D, Öztürk M, Yılmazer S. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. J Chem Neuroanat. 2017;80:19-26 pubmed publisher
  4. López de Maturana R, Lang V, Zubiarrain A, Sousa A, Vázquez N, Gorostidi A, et al. Mutations in LRRK2 impair NF-κB pathway in iPSC-derived neurons. J Neuroinflammation. 2016;13:295 pubmed
  5. Peng Y, Kim M, Hullinger R, O Riordan K, Burger C, Pehar M, et al. Improved proteostasis in the secretory pathway rescues Alzheimer's disease in the mouse. Brain. 2016;139:937-52 pubmed publisher
  6. Taniguchi Watanabe S, Arai T, Kametani F, Nonaka T, Masuda Suzukake M, Tarutani A, et al. Biochemical classification of tauopathies by immunoblot, protein sequence and mass spectrometric analyses of sarkosyl-insoluble and trypsin-resistant tau. Acta Neuropathol. 2016;131:267-280 pubmed publisher
  7. Corbel C, Zhang B, Le Parc A, Baratte B, Colas P, Couturier C, et al. Tamoxifen inhibits CDK5 kinase activity by interacting with p35/p25 and modulates the pattern of tau phosphorylation. Chem Biol. 2015;22:472-482 pubmed publisher
  8. Takeuchi R, Toyoshima Y, Tada M, Tanaka H, Shimizu H, Shiga A, et al. Globular Glial Mixed Four Repeat Tau and TDP-43 Proteinopathy with Motor Neuron Disease and Frontotemporal Dementia. Brain Pathol. 2016;26:82-94 pubmed publisher
  9. Melis V, Zabke C, Stamer K, Magbagbeolu M, Schwab K, Marschall P, et al. Different pathways of molecular pathophysiology underlie cognitive and motor tauopathy phenotypes in transgenic models for Alzheimer's disease and frontotemporal lobar degeneration. Cell Mol Life Sci. 2015;72:2199-222 pubmed publisher