This is a Validated Antibody Database (VAD) review about pig PHB, based on 9 published articles (read how Labome selects the articles), using PHB antibody in all methods. It is aimed to help Labome visitors find the most suited PHB antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PHB synonym: prohibitin

Invitrogen
mouse monoclonal (II-14-10)
  • immunohistochemistry - paraffin section; human; loading ...; fig 2b
In order to show that PHB1 is highly expressed in non-small cell lung cancers patients and correlates with poor survival, Invitrogen PHB antibody (Thermo Fisher, MA5-12858) was used in immunohistochemistry - paraffin section on human samples (fig 2b). Oncogene (2017) ncbi
mouse monoclonal (II-14-10)
  • western blot; human; loading ...; fig 2c
In order to identify components of mitochondrial RNA granules, Invitrogen PHB antibody (Thermo Fisher Scientific, MA5-12858) was used in western blot on human samples (fig 2c). J Biol Chem (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig 2h
  • western blot; human; 1:500; loading ...; fig 1b
In order to recognize the inner mitochondrial membrane protein, prohibitin 2, as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation, Invitrogen PHB antibody (ThermoFisher Scientific, PA5-19556) was used in western blot on mouse samples at 1:500 (fig 2h) and in western blot on human samples at 1:500 (fig 1b). Cell (2017) ncbi
mouse monoclonal (II-14-10)
  • western blot; human; loading ...; fig 2b
In order to examine the role of tissue transglutaminase 2 in the biogenesis of exosomes, Invitrogen PHB antibody (Lab Vision, MS-261-PABX) was used in western blot on human samples (fig 2b). Biochim Biophys Acta (2016) ncbi
mouse monoclonal (II-14-10)
  • western blot; rat; 1:250; fig 5a
In order to identify novel molecules in peripheral myelination by spatial mapping of juxtacrine axo-glial interactions, Invitrogen PHB antibody (Thermo Scientific, II-14-10) was used in western blot on rat samples at 1:250 (fig 5a). Nat Commun (2015) ncbi
mouse monoclonal (II-14-10)
  • western blot; pig; 1:100
In order to study hepatitis E pathogenesis and virulence mechanisms, Invitrogen PHB antibody (Thermo, MA512858) was used in western blot on pig samples at 1:100. J Virol (2015) ncbi
mouse monoclonal (II-14-10)
  • immunohistochemistry - paraffin section; human; 1:100
In order to study how the 3' UTR 1630 C T polymorphism and copy number variation are associated with Prohibitin expression deregulation in gastric cancer, Invitrogen PHB antibody (ThermoFisher, II-14-10) was used in immunohistochemistry - paraffin section on human samples at 1:100. PLoS ONE (2014) ncbi
mouse monoclonal (II-14-10)
  • western blot; human
In order to study the role of prohibitins as endogenous Siglec-9 ligands that inhibit ERK signaling and IL-12 production by Jurkat cells, Invitrogen PHB antibody (Neo Markers, II-14-10) was used in western blot on human samples . Biochem Biophys Res Commun (2013) ncbi
mouse monoclonal (II-14-10)
  • western blot; human; fig 5
In order to investigate the role of brain and reproductive organ-expressed protein BRE in death receptor-mediated apoptosis, Invitrogen PHB antibody (Lab Vision, Ab-1) was used in western blot on human samples (fig 5). J Biol Chem (2004) ncbi
Articles Reviewed
  1. Yurugi H, Marini F, Weber C, David K, Zhao Q, Binder H, et al. Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours. Oncogene. 2017;36:4778-4789 pubmed publisher
  2. Zaganelli S, Rebelo Guiomar P, Maundrell K, Rozanska A, Pierredon S, Powell C, et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem. 2017;292:4519-4532 pubmed publisher
  3. Wei Y, Chiang W, Sumpter R, Mishra P, Levine B. Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor. Cell. 2017;168:224-238.e10 pubmed publisher
  4. Diaz Hidalgo L, Altuntas S, Rossin F, D Eletto M, Marsella C, Farrace M, et al. Transglutaminase type 2-dependent selective recruitment of proteins into exosomes under stressful cellular conditions. Biochim Biophys Acta. 2016;1863:2084-92 pubmed publisher
  5. Poitelon Y, Bogni S, Matafora V, Della Flora Nunes G, Hurley E, Ghidinelli M, et al. Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Nat Commun. 2015;6:8303 pubmed publisher
  6. Rogée S, Le Gall M, Chafey P, Bouquet J, Cordonnier N, Frederici C, et al. Quantitative proteomics identifies host factors modulated during acute hepatitis E virus infection in the swine model. J Virol. 2015;89:129-43 pubmed publisher
  7. Leal M, Cirilo P, Mazzotti T, Calcagno D, Wisnieski F, Demachki S, et al. Prohibitin expression deregulation in gastric cancer is associated with the 3' untranslated region 1630 C>T polymorphism and copy number variation. PLoS ONE. 2014;9:e98583 pubmed publisher
  8. Yurugi H, Tanida S, Akita K, Ishida A, Toda M, Nakada H. Prohibitins function as endogenous ligands for Siglec-9 and negatively regulate TCR signaling upon ligation. Biochem Biophys Res Commun. 2013;434:376-81 pubmed publisher
  9. Li Q, Ching A, Chan B, Chow S, Lim P, Ho T, et al. A death receptor-associated anti-apoptotic protein, BRE, inhibits mitochondrial apoptotic pathway. J Biol Chem. 2004;279:52106-16 pubmed