This is a Validated Antibody Database (VAD) review about pig GFAP, based on 21 published articles (read how Labome selects the articles), using GFAP antibody in all methods. It is aimed to help Labome visitors find the most suited GFAP antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GFAP synonym: glial fibrillary acidic protein

Invitrogen
mouse monoclonal (GA5)
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 3a
Invitrogen GFAP antibody (Thermo Fisher Scientific, 14-9892-82) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 3a). Neuron (2019) ncbi
mouse monoclonal (ASTRO6)
  • immunocytochemistry; mouse; loading ...; fig s1b
In order to investigate the involvement of HspBP1 in the neuronal susceptibility towards misfolded proteins, Invitrogen GFAP antibody (Thermo, MA5-12023) was used in immunocytochemistry on mouse samples (fig s1b). Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig S2G
In order to investigate the role of Cxcr5 receptor in age-related macular degeneration in mice., Invitrogen GFAP antibody (invitrogen, PA1-10019) was used in immunohistochemistry - frozen section on mouse samples (fig S2G). PLoS ONE (2017) ncbi
chicken polyclonal
  • immunohistochemistry; mouse; loading ...; fig 7a
In order to report that Tbx18 selectively marks pericytes and vascular smooth muscle cells, Invitrogen GFAP antibody (ThermoFisher, PA1-10004) was used in immunohistochemistry on mouse samples (fig 7a). Cell Stem Cell (2017) ncbi
mouse monoclonal (ASTRO6)
  • immunohistochemistry - frozen section; human; 1:500; fig 5e
In order to identify an astrocyte subtype induced by activated microglia, Invitrogen GFAP antibody (Invitrogen, MA5-12023) was used in immunohistochemistry - frozen section on human samples at 1:500 (fig 5e). Nature (2017) ncbi
mouse monoclonal (ASTRO6)
  • immunocytochemistry; Epinephelus; fig 1a
In order to study neuronal death caused by nervous necrosis virus infection, Invitrogen GFAP antibody (Thermo Fisher Scientific, MA5-12023) was used in immunocytochemistry on Epinephelus samples (fig 1a). Dev Comp Immunol (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:1000; fig 6b
In order to optimize the differentiation of human pluripotent embryonal carcinoma cells, Invitrogen GFAP antibody (ThermoFisher Scientific, PA3-16727) was used in immunocytochemistry on human samples at 1:1000 (fig 6b). Dev Growth Differ (2016) ncbi
mouse monoclonal (ASTRO6)
  • immunohistochemistry; mouse; 1:2000; fig 2C
In order to study the amelioration of neuropathic pain by down-regulating spinal IL-1-beta via suppressing JAK2-STAT3 signaling and astroglial NALP1 inflammasome by curcumin, Invitrogen GFAP antibody (Thermo, MA5-12023) was used in immunohistochemistry on mouse samples at 1:2000 (fig 2C). Sci Rep (2016) ncbi
chicken polyclonal
  • immunohistochemistry - free floating section; rat; 1:2000; fig 4
In order to characterize how astrocytes respond to dopaminergic denervation of the striatum, Invitrogen GFAP antibody (Thermo Scientific, PA1-10004) was used in immunohistochemistry - free floating section on rat samples at 1:2000 (fig 4). J Neurochem (2016) ncbi
mouse monoclonal (ASTRO6)
  • immunohistochemistry - paraffin section; mouse; fig 4
In order to measure LCN2 in ischemic samples, Invitrogen GFAP antibody (Thermo Scientific, MS-1376) was used in immunohistochemistry - paraffin section on mouse samples (fig 4). PLoS ONE (2016) ncbi
mouse monoclonal (ASTRO6)
  • immunohistochemistry - paraffin section; human; 1:100; fig 4
In order to study a case report and literature review of recurrent intracranial neurenteric cyst with malignant transformation, Invitrogen GFAP antibody (Thermo Fisher, MA5-12023) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 4). Oncol Lett (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; fig 6
  • western blot; mouse; fig 4
In order to study experimental traumatic brain injury and the role of aberrant Cdk5/p25 activity, Invitrogen GFAP antibody (Pierce, PA3-16727) was used in immunohistochemistry - paraffin section on mouse samples (fig 6) and in western blot on mouse samples (fig 4). J Neurochem (2016) ncbi
mouse monoclonal (GA5)
  • immunohistochemistry - paraffin section; human; loading ...; fig 3f
In order to discuss the role of the estrogen receptor in Alzheimer disease pathogenesis, Invitrogen GFAP antibody (Invitrogen, GA5) was used in immunohistochemistry - paraffin section on human samples (fig 3f). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; fig 7
In order to characterize tau pathology mouse models via obesity, diabetes, and leptin resistance, Invitrogen GFAP antibody (Pierce, PA1-10019) was used in immunohistochemistry on mouse samples at 1:1000 (fig 7). Neuroscience (2016) ncbi
mouse monoclonal (ASTRO6)
  • immunohistochemistry - frozen section; mouse; 1:2000; fig 3
  • western blot; mouse; 1:5000; fig 7
In order to determine the correlation between neuronal caMKII-CREB and astroglial JAK2-STAT3 cascades in mice and how spinal IL-33/ST2 signaling contributes to neuropathic pain, Invitrogen GFAP antibody (Thermo Scientific, MA5-12023) was used in immunohistochemistry - frozen section on mouse samples at 1:2000 (fig 3) and in western blot on mouse samples at 1:5000 (fig 7). Anesthesiology (2015) ncbi
rabbit polyclonal
  • western blot; rat
In order to examine the role of Trx family proteins in neuronal development and recovery after hypoxia/ischemia and reperfusion, Invitrogen GFAP antibody (thermo, pa3-16727) was used in western blot on rat samples . Biochim Biophys Acta (2015) ncbi
mouse monoclonal (ASTRO6)
  • immunohistochemistry - paraffin section; mouse; fig 5
In order to use Fth and Ftl knock out mice to study iron metabolism in the brain, Invitrogen GFAP antibody (Thermo, ASTRO6) was used in immunohistochemistry - paraffin section on mouse samples (fig 5). PLoS ONE (2015) ncbi
mouse monoclonal (ASTRO6)
  • immunohistochemistry - paraffin section; rat
In order to test if rhodiola rosea extract stimulates neuronal stem cell proliferation and improves depression, Invitrogen GFAP antibody (Lab Vision, MS-1376-P) was used in immunohistochemistry - paraffin section on rat samples . Int J Stem Cells (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:5000
In order to test the effect of a p38alpha MAPK inhibitor on spinal cord injury in the rat, Invitrogen GFAP antibody (ThermoScientific, PA3-16727) was used in immunohistochemistry - free floating section on rat samples at 1:5000. Pain (2014) ncbi
mouse monoclonal (GFA-02)
  • flow cytometry; mouse
In order to elucidate chromatin activation and epigenetic regulation and their mark on H3K4me3 in primate progenitor cells within adult neurogenic niche, Invitrogen GFAP antibody (Pierce, MA1-35376) was used in flow cytometry on mouse samples . Sci Rep (2014) ncbi
chicken polyclonal
  • immunohistochemistry; mouse; 1:500
In order to study the role of ASIC1 and ASIC2 channels in the defensive response to aversive stimuli, Invitrogen GFAP antibody (Thermo Fisher Scientific , PA1-10004) was used in immunohistochemistry on mouse samples at 1:500. Genes Brain Behav (2014) ncbi
Articles Reviewed
  1. Yang J, Vitery M, Chen J, Osei Owusu J, Chu J, Qiu Z. Glutamate-Releasing SWELL1 Channel in Astrocytes Modulates Synaptic Transmission and Promotes Brain Damage in Stroke. Neuron. 2019;102:813-827.e6 pubmed publisher
  2. Zhao T, Hong Y, Yin P, Li S, Li X. Differential HspBP1 expression accounts for the greater vulnerability of neurons than astrocytes to misfolded proteins. Proc Natl Acad Sci U S A. 2017;114:E7803-E7811 pubmed publisher
  3. Huang H, Liu Y, Wang L, Li W. Age-related macular degeneration phenotypes are associated with increased tumor necrosis-alpha and subretinal immune cells in aged Cxcr5 knockout mice. PLoS ONE. 2017;12:e0173716 pubmed publisher
  4. Guimarães Camboa N, Cattaneo P, Sun Y, Moore Morris T, Gu Y, Dalton N, et al. Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In Vivo. Cell Stem Cell. 2017;20:345-359.e5 pubmed publisher
  5. Liddelow S, Guttenplan K, Clarke L, Bennett F, Bohlen C, Schirmer L, et al. Neurotoxic reactive astrocytes are induced by activated microglia. Nature. 2017;541:481-487 pubmed publisher
  6. Chiang Y, Wu Y, Chi S. Interleukin-1β secreted from betanodavirus-infected microglia caused the death of neurons in giant grouper brains. Dev Comp Immunol. 2017;70:19-26 pubmed publisher
  7. Abolpour Mofrad S, Kuenzel K, Friedrich O, Gilbert D. Optimizing neuronal differentiation of human pluripotent NT2 stem cells in monolayer cultures. Dev Growth Differ. 2016;58:664-676 pubmed publisher
  8. Liu S, Li Q, Zhang M, Mao Ying Q, Hu L, Wu G, et al. Curcumin ameliorates neuropathic pain by down-regulating spinal IL-1β via suppressing astroglial NALP1 inflammasome and JAK2-STAT3 signalling. Sci Rep. 2016;6:28956 pubmed publisher
  9. Morales I, Sánchez A, Rodriguez Sabate C, Rodriguez M. The astrocytic response to the dopaminergic denervation of the striatum. J Neurochem. 2016;139:81-95 pubmed publisher
  10. Hochmeister S, Engel O, Adzemovic M, Pekar T, Kendlbacher P, Zeitelhofer M, et al. Lipocalin-2 as an Infection-Related Biomarker to Predict Clinical Outcome in Ischemic Stroke. PLoS ONE. 2016;11:e0154797 pubmed publisher
  11. Yang Y, Fang J, Li D, Wang L, Ji N, Zhang J. Recurrent intracranial neurenteric cyst with malignant transformation: A case report and literature review. Oncol Lett. 2016;11:3395-3402 pubmed
  12. Yousuf M, Tan C, Torres Altoro M, Lu F, Plautz E, Zhang S, et al. Involvement of aberrant cyclin-dependent kinase 5/p25 activity in experimental traumatic brain injury. J Neurochem. 2016;138:317-27 pubmed publisher
  13. Wang C, Zhang F, Jiang S, Siedlak S, Shen L, Perry G, et al. Estrogen receptor-? is localized to neurofibrillary tangles in Alzheimer's disease. Sci Rep. 2016;6:20352 pubmed publisher
  14. Platt T, Beckett T, Kohler K, Niedowicz D, Murphy M. Obesity, diabetes, and leptin resistance promote tau pathology in a mouse model of disease. Neuroscience. 2016;315:162-74 pubmed publisher
  15. Liu S, Mi W, Li Q, Zhang M, Han P, Hu S, et al. Spinal IL-33/ST2 Signaling Contributes to Neuropathic Pain via Neuronal CaMKII-CREB and Astroglial JAK2-STAT3 Cascades in Mice. Anesthesiology. 2015;123:1154-69 pubmed publisher
  16. Romero J, Hanschmann E, Gellert M, Eitner S, Holubiec M, Blanco Calvo E, et al. Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia. Biochim Biophys Acta. 2015;1850:1274-85 pubmed publisher
  17. Li W, Garringer H, GOODWIN C, Richine B, Acton A, Vanduyn N, et al. Systemic and cerebral iron homeostasis in ferritin knock-out mice. PLoS ONE. 2015;10:e0117435 pubmed publisher
  18. Kamel Ismail Z, Morcos M, Eldin Mohammad M, Gamal Aboulkhair A. Enhancement of Neural Stem Cells after Induction of Depression in Male Albino Rats (A histological & Immunohistochemical Study). Int J Stem Cells. 2014;7:70-8 pubmed publisher
  19. Galán Arriero I, Avila Martin G, Ferrer Donato A, Gómez Soriano J, Bravo Esteban E, Taylor J. Oral administration of the p38α MAPK inhibitor, UR13870, inhibits affective pain behavior after spinal cord injury. Pain. 2014;155:2188-98 pubmed publisher
  20. Sandstrom R, Foret M, Grow D, Haugen E, Rhodes C, Cardona A, et al. Epigenetic regulation by chromatin activation mark H3K4me3 in primate progenitor cells within adult neurogenic niche. Sci Rep. 2014;4:5371 pubmed publisher
  21. Price M, Gong H, Parsons M, Kundert J, Reznikov L, Bernardinelli L, et al. Localization and behaviors in null mice suggest that ASIC1 and ASIC2 modulate responses to aversive stimuli. Genes Brain Behav. 2014;13:179-94 pubmed publisher