This is a Validated Antibody Database (VAD) review about pig GAPDH, based on 17 published articles (read how Labome selects the articles), using GAPDH antibody in all methods. It is aimed to help Labome visitors find the most suited GAPDH antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GAPDH synonym: GAPD; glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH

Invitrogen
mouse monoclonal (1D4)
  • western blot; rat; 1:40,000; loading ...
In order to test if increasing the transcription factor cAMP response element-binding protein expression also ameliorates age-related behavioral and biophysical deficits, Invitrogen GAPDH antibody (Thermo Fischer Scientific, MA1-16757) was used in western blot on rat samples at 1:40,000. elife (2017) ncbi
mouse monoclonal (1D4)
  • western blot; mouse; loading ...; fig 4
In order to evaluate the antifibrotic effect of emodin in silica inhalation-induced lung fibrosis, Invitrogen GAPDH antibody (Invitrogen, MA1-16757) was used in western blot on mouse samples (fig 4). Mol Med Rep (2016) ncbi
mouse monoclonal (258)
  • western blot; human; fig s2d
In order to study p53 aggregation and p53 tumor suppression in ovarian carcinomas, Invitrogen GAPDH antibody (Invitrogen, 258) was used in western blot on human samples (fig s2d). Cancer Cell (2016) ncbi
mouse monoclonal (258)
  • western blot; human; 1:10,000; fig 7
In order to study how Nck signaling contributes to vascular formation and remodeling, Invitrogen GAPDH antibody (Invitrogen, 437000) was used in western blot on human samples at 1:10,000 (fig 7). Mol Biol Cell (2015) ncbi
mouse monoclonal (1D4)
  • western blot; human
In order to correlate epigenomic changes with tumor aggressiveness, Invitrogen GAPDH antibody (Thermo Scientific, MA1-16757) was used in western blot on human samples . Int J Cancer (2015) ncbi
mouse monoclonal (258)
  • western blot; mouse; 1:10000
In order to study the susceptibility of synaptic mitochondria to damage, Invitrogen GAPDH antibody (Invitrogen, 437000) was used in western blot on mouse samples at 1:10000. J Proteome Res (2014) ncbi
mouse monoclonal (258)
  • western blot; human; 1:10000; fig 3
In order to examine the impact of oxygen levels on mitochondrial function in neurons, Invitrogen GAPDH antibody (Invitrogen, 437000) was used in western blot on human samples at 1:10000 (fig 3). J Proteome Res (2013) ncbi
mouse monoclonal (258)
  • western blot; human; 1:10000; fig 4
In order to elucidate the role of Nck in cytoskeletal remodeling during directional migration, Invitrogen GAPDH antibody (Invitrogen, 437000) was used in western blot on human samples at 1:10000 (fig 4). J Cell Sci (2013) ncbi
mouse monoclonal (258)
  • western blot; human; fig 5
In order to develop a TR-FRET immuno-assay for the quantitative measurement of autophagy using GFP-tagged LC3B, Invitrogen GAPDH antibody (Life Technologies, 437000) was used in western blot on human samples (fig 5). Autophagy (2012) ncbi
Bio-Rad
mouse monoclonal (4G5)
  • western blot; human; 1:50,000; fig 7
Bio-Rad GAPDH antibody (ABD Serotec, MCA4740) was used in western blot on human samples at 1:50,000 (fig 7). Nat Commun (2016) ncbi
mouse monoclonal (6C5)
  • western blot; human; 1:1000; fig 7
Bio-Rad GAPDH antibody (Bio-Rad, MCA4739) was used in western blot on human samples at 1:1000 (fig 7). PLoS ONE (2016) ncbi
mouse monoclonal (6C5)
  • western blot; mouse; 1:2000; fig 2
Bio-Rad GAPDH antibody (ABD Serotec, MCA4739) was used in western blot on mouse samples at 1:2000 (fig 2). Nat Commun (2016) ncbi
mouse monoclonal (4G5)
  • western blot; human; fig 5c
Bio-Rad GAPDH antibody (AbD Serotec, MCA4740) was used in western blot on human samples (fig 5c). Nucleic Acids Res (2016) ncbi
mouse monoclonal (6C5)
  • western blot; mouse; fig 6
In order to study the role of Rpn10 and Rpn13 in recognition of cellular homeostasis and ubiquitinated protein, Bio-Rad GAPDH antibody (AbD Serotec, MCA4739) was used in western blot on mouse samples (fig 6). PLoS Genet (2015) ncbi
mouse monoclonal (4G5)
  • western blot; human
In order to study OGR1-mediated, pH-dependent signaling pathways in intestinal epithelial cells, Bio-Rad GAPDH antibody (AbD Serotec, MCA4740) was used in western blot on human samples . Am J Physiol Gastrointest Liver Physiol (2015) ncbi
mouse monoclonal (4G5)
  • immunocytochemistry; human
Bio-Rad GAPDH antibody (AbD Serotech, 4G5) was used in immunocytochemistry on human samples . Eur J Cell Biol (2014) ncbi
GeneTex
mouse monoclonal (1D4)
  • western blot; mouse; 1:1000; fig s4
GeneTex GAPDH antibody (GeneTex, 1D4) was used in western blot on mouse samples at 1:1000 (fig s4). Nature (2015) ncbi
Articles Reviewed
  1. Yu X, Curlik D, Oh M, Yin J, Disterhoft J. CREB overexpression in dorsal CA1 ameliorates long-term memory deficits in aged rats. elife. 2017;6: pubmed publisher
  2. Yang T, Wang J, Pang Y, Dang X, Ren H, Liu Y, et al. Emodin suppresses silica-induced lung fibrosis by promoting Sirt1 signaling via direct contact. Mol Med Rep. 2016;14:4643-4649 pubmed publisher
  3. Pagliuso A, Valente C, Giordano L, Filograna A, Li G, Circolo D, et al. Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase ?. Nat Commun. 2016;7:12148 pubmed publisher
  4. Choi H, Jin S, Kwon J, Kim J, Jeong J, Kim J, et al. Characterization of Mammalian ADAM2 and Its Absence from Human Sperm. PLoS ONE. 2016;11:e0158321 pubmed publisher
  5. Gautheron J, Vucur M, Schneider A, Severi I, Roderburg C, Roy S, et al. The necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance. Nat Commun. 2016;7:11869 pubmed publisher
  6. Soragni A, Janzen D, Johnson L, Lindgren A, Thai Quynh Nguyen A, Tiourin E, et al. A Designed Inhibitor of p53 Aggregation Rescues p53 Tumor Suppression in Ovarian Carcinomas. Cancer Cell. 2016;29:90-103 pubmed publisher
  7. Baude A, Aaes T, Zhai B, Al Nakouzi N, Oo H, Daugaard M, et al. Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination. Nucleic Acids Res. 2016;44:2214-26 pubmed publisher
  8. Hamazaki J, Hirayama S, Murata S. Redundant Roles of Rpn10 and Rpn13 in Recognition of Ubiquitinated Proteins and Cellular Homeostasis. PLoS Genet. 2015;11:e1005401 pubmed publisher
  9. de Vallière C, Vidal S, Clay I, Jurisic G, Tcymbarevich I, Lang S, et al. The pH-sensing receptor OGR1 improves barrier function of epithelial cells and inhibits migration in an acidic environment. Am J Physiol Gastrointest Liver Physiol. 2015;309:G475-90 pubmed publisher
  10. Chaki S, Barhoumi R, Rivera G. Actin remodeling by Nck regulates endothelial lumen formation. Mol Biol Cell. 2015;26:3047-60 pubmed publisher
  11. Xu G, Chapman J, Brandsma I, Yuan J, Mistrik M, Bouwman P, et al. REV7 counteracts DNA double-strand break resection and affects PARP inhibition. Nature. 2015;521:541-544 pubmed publisher
  12. Bulk E, Ay A, Hammadi M, Ouadid Ahidouch H, Schelhaas S, Hascher A, et al. Epigenetic dysregulation of KCa 3.1 channels induces poor prognosis in lung cancer. Int J Cancer. 2015;137:1306-17 pubmed publisher
  13. García E, Machesky L, Jones G, Antón I. WIP is necessary for matrix invasion by breast cancer cells. Eur J Cell Biol. 2014;93:413-23 pubmed publisher
  14. Stauch K, Purnell P, Fox H. Quantitative proteomics of synaptic and nonsynaptic mitochondria: insights for synaptic mitochondrial vulnerability. J Proteome Res. 2014;13:2620-36 pubmed publisher
  15. Villeneuve L, Tiede L, Morsey B, Fox H. Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone. J Proteome Res. 2013;12:4599-606 pubmed publisher
  16. Chaki S, Barhoumi R, Berginski M, Sreenivasappa H, Trache A, Gomez S, et al. Nck enables directional cell migration through the coordination of polarized membrane protrusion with adhesion dynamics. J Cell Sci. 2013;126:1637-49 pubmed publisher
  17. Hancock M, Hermanson S, Dolman N. A quantitative TR-FRET plate reader immunoassay for measuring autophagy. Autophagy. 2012;8:1227-44 pubmed publisher