This is a Validated Antibody Database (VAD) review about pig AP2A1, based on 5 published articles (read how Labome selects the articles), using AP2A1 antibody in all methods. It is aimed to help Labome visitors find the most suited AP2A1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AP2A1 synonym: AP-2 complex subunit alpha-1; adaptor related protein complex 2 alpha 1 subunit

Invitrogen
mouse monoclonal (AC1-M11)
  • western blot; mouse; loading ...; fig 3a
Invitrogen AP2A1 antibody (Dianova, MA3-061) was used in western blot on mouse samples (fig 3a). Neuroscience (2018) ncbi
mouse monoclonal (AC1-M11)
  • immunoprecipitation; human; fig s1a
  • western blot; human; fig s1a
In order to elucidate how adaptor protein 2 complexes regulate clathrin-coated pit assembly, Invitrogen AP2A1 antibody (Thermo Fisher, AC1-M11) was used in immunoprecipitation on human samples (fig s1a) and in western blot on human samples (fig s1a). J Cell Biol (2017) ncbi
mouse monoclonal (AC1-M11)
  • immunocytochemistry; human; loading ...; fig 5a
  • western blot; human; loading ...; fig 4b
In order to propose that ikarugamycin is a useful reagent to study routes of endocytic trafficking, Invitrogen AP2A1 antibody (Thermo Scientific, MA3-061) was used in immunocytochemistry on human samples (fig 5a) and in western blot on human samples (fig 4b). Traffic (2016) ncbi
mouse monoclonal (AC1-M11)
  • western blot; rat; loading ...; fig 3a
In order to find factors that interact with N-myristoylated Arf6, Invitrogen AP2A1 antibody (Affinity Bioreagents, AC1-M11) was used in western blot on rat samples (fig 3a). Methods Enzymol (2005) ncbi
mouse monoclonal (AC1-M11)
  • western blot; mouse; loading ...; fig 2c
In order to explore the effects of tensile stress on osteoblast differentiation and osteogenesis, Invitrogen AP2A1 antibody (Affinity Bioreagents, AC1-M11) was used in western blot on mouse samples (fig 2c). J Cell Physiol (2003) ncbi
Articles Reviewed
  1. Richter K, Schmutz I, Darna M, Zander J, Chavan R, Albrecht U, et al. VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context. Neuroscience. 2018;371:29-37 pubmed publisher
  2. Kadlecova Z, Spielman S, Loerke D, Mohanakrishnan A, Reed D, Schmid S. Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2. J Cell Biol. 2017;216:167-179 pubmed publisher
  3. Elkin S, Oswald N, Reed D, Mettlen M, Macmillan J, Schmid S. Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis. Traffic. 2016;17:1139-49 pubmed publisher
  4. Krauss M, Haucke V. Functional assay of effectors of ADP ribosylation factor 6 during clathrin/AP-2 coat recruitment to membranes. Methods Enzymol. 2005;404:388-98 pubmed
  5. Shimomura J, Ishibashi O, Ikegame M, Yoshizawa T, Ejiri S, Noda T, et al. Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation. J Cell Physiol. 2003;195:488-96 pubmed