This is a Validated Antibody Database (VAD) review about mouse Wt1, based on 20 published articles (read how Labome selects the articles), using Wt1 antibody in all methods. It is aimed to help Labome visitors find the most suited Wt1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Wt1 synonym: D630046I19Rik; Wt-1

Santa Cruz Biotechnology
mouse monoclonal (F-6)
  • western blot; mouse; 1:750; loading ...; fig 3e
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz, sc7385) was used in western blot on mouse samples at 1:750 (fig 3e). EMBO Mol Med (2019) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry - paraffin section; rat; loading ...; fig s6
Santa Cruz Biotechnology Wt1 antibody (SantaCruz, sc-7385) was used in immunohistochemistry - paraffin section on rat samples (fig s6). J Clin Invest (2017) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry; mouse; loading ...; fig 2a
In order to perform whole exome sequencing to identify recessive causes of steroid-resistant nephrotic syndrome, Santa Cruz Biotechnology Wt1 antibody (Santa Cruz, sc-7385) was used in immunohistochemistry on mouse samples (fig 2a). J Clin Invest (2017) ncbi
mouse monoclonal (F-6)
  • chromatin immunoprecipitation; human; loading ...; fig 3a
  • western blot; human; loading ...; fig 1d
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz Biotechnology, F-6) was used in chromatin immunoprecipitation on human samples (fig 3a) and in western blot on human samples (fig 1d). Biochem Biophys Res Commun (2017) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry - paraffin section; mouse; fig 2
  • western blot; mouse; fig 1
In order to analyze infertility in mice by deletion of the tyrosine phosphatase Shp2 in Sertoli cells, Santa Cruz Biotechnology Wt1 antibody (Santa Cruz Biotechnology, sc-7385) was used in immunohistochemistry - paraffin section on mouse samples (fig 2) and in western blot on mouse samples (fig 1). Sci Rep (2015) ncbi
mouse monoclonal (F-6)
  • western blot; mouse; loading ...; fig 4g
In order to develop and characterize a mouse model of glomerular disease, Santa Cruz Biotechnology Wt1 antibody (Santa Cruz, sc-7385) was used in western blot on mouse samples (fig 4g). J Clin Invest (2015) ncbi
mouse monoclonal (F-6)
  • immunoprecipitation; human; fig 2b
  • immunocytochemistry; human; fig 2c
  • western blot; human; 1:1000; fig 6e
  • immunoprecipitation; mouse; fig 2d
  • western blot; mouse; 1:1000; fig 6e
Santa Cruz Biotechnology Wt1 antibody (Santa, sc-7385) was used in immunoprecipitation on human samples (fig 2b), in immunocytochemistry on human samples (fig 2c), in western blot on human samples at 1:1000 (fig 6e), in immunoprecipitation on mouse samples (fig 2d) and in western blot on mouse samples at 1:1000 (fig 6e). Nat Commun (2014) ncbi
mouse monoclonal (F-6)
  • immunohistochemistry; mouse; 1:1000; fig 6
Santa Cruz Biotechnology Wt1 antibody (Santa Cruz, sc-7385) was used in immunohistochemistry on mouse samples at 1:1000 (fig 6). Stem Cells (2010) ncbi
Abcam
domestic rabbit monoclonal (CAN-R9(IHC)-56-2)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1h
Abcam Wt1 antibody (Abcam, ab89901) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1h). Nature (2020) ncbi
domestic rabbit monoclonal (CAN-R9(IHC)-56-2)
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 1a
Abcam Wt1 antibody (Abcam, ab89901) was used in immunohistochemistry - paraffin section on mouse samples at 1:1000 (fig 1a). Sci Rep (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:300; loading ...; fig s1a
Abcam Wt1 antibody (Abcam, ab15249) was used in immunohistochemistry on mouse samples at 1:300 (fig s1a). Science (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; loading ...; fig 5a
Abcam Wt1 antibody (Abcam, ab15249) was used in immunohistochemistry - paraffin section on rat samples (fig 5a). Oncotarget (2016) ncbi
domestic rabbit monoclonal (CAN-R9(IHC)-56-2)
  • immunocytochemistry; mouse; 1:500; fig 3
Abcam Wt1 antibody (Abcam, ab899901) was used in immunocytochemistry on mouse samples at 1:500 (fig 3). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (CAN-R9(IHC)-56-2)
  • immunohistochemistry; mouse; 1:50; fig 6b
Abcam Wt1 antibody (Epitomics (now Abcam), ab89901) was used in immunohistochemistry on mouse samples at 1:50 (fig 6b). Endocrinology (2015) ncbi
domestic rabbit monoclonal (CAN-R9(IHC)-56-2)
  • immunohistochemistry; mouse
Abcam Wt1 antibody (Abcam, ab89901) was used in immunohistochemistry on mouse samples . Endocrinology (2015) ncbi
Invitrogen
mouse monoclonal (6F-H2)
  • immunohistochemistry - paraffin section; human; fig 1
In order to measure Wilms' tumor 1 protein expression in endometrial adenocarcinoma and endometrial intra-epithelial neoplasia, Invitrogen Wt1 antibody (Thermo Fisher, 6F-H2) was used in immunohistochemistry - paraffin section on human samples (fig 1). J Obstet Gynaecol Res (2016) ncbi
mouse monoclonal (6F-H2)
  • immunocytochemistry; mouse; fig 2
In order to study ex vivo assessment and epicardial outgrowth culture assay of epicardial-derived cell migration, Invitrogen Wt1 antibody (Life Technologies, MA1-46028) was used in immunocytochemistry on mouse samples (fig 2). J Vis Exp (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; rat; fig 5
In order to assess immunohistochemistry markers using four different acute and chronic rat kidney disease models, Invitrogen Wt1 antibody (Thermo Scientific, PA1-20991) was used in immunohistochemistry on rat samples (fig 5). Histochem Cell Biol (2016) ncbi
mouse monoclonal (6F-H2)
  • immunohistochemistry - paraffin section; human
In order to compare meningiomas and mesotheliomas immunohistochemically, Invitrogen Wt1 antibody (Thermo Scientific, 6F-H2) was used in immunohistochemistry - paraffin section on human samples . Biomed Res Int (2014) ncbi
Novus Biologicals
mouse monoclonal (6F-H2)
  • western blot; human; 1:400; loading ...; fig 2e
Novus Biologicals Wt1 antibody (Novus Biologicals, NB-110?C60011) was used in western blot on human samples at 1:400 (fig 2e). Int J Cancer (2015) ncbi
Articles Reviewed
  1. Wan L, Chong S, Xuan F, Liang A, Cui X, Gates L, et al. Impaired cell fate through gain-of-function mutations in a chromatin reader. Nature. 2020;577:121-126 pubmed publisher
  2. Lin C, Hsu Y, Huang Y, Shih Y, Wang C, Chiang W, et al. A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction. EMBO Mol Med. 2019;11: pubmed publisher
  3. Taguchi K, Yamagishi S, Yokoro M, Ito S, Kodama G, Kaida Y, et al. RAGE-aptamer attenuates deoxycorticosterone acetate/salt-induced renal injury in mice. Sci Rep. 2018;8:2686 pubmed publisher
  4. Rao J, Ashraf S, Tan W, van der Ven A, Gee H, Braun D, et al. Advillin acts upstream of phospholipase C ϵ1 in steroid-resistant nephrotic syndrome. J Clin Invest. 2017;127:4257-4269 pubmed publisher
  5. Zhao F, Franco H, Rodriguez K, Brown P, Tsai M, Tsai S, et al. Elimination of the male reproductive tract in the female embryo is promoted by COUP-TFII in mice. Science. 2017;357:717-720 pubmed publisher
  6. Lovric S, Gonçalves S, Gee H, Oskouian B, Srinivas H, Choi W, et al. Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency. J Clin Invest. 2017;127:912-928 pubmed publisher
  7. Ullmark T, Montano G, Järvstråt L, Jernmark Nilsson H, Håkansson E, Drott K, et al. Anti-apoptotic quinolinate phosphoribosyltransferase (QPRT) is a target gene of Wilms' tumor gene 1 (WT1) protein in leukemic cells. Biochem Biophys Res Commun. 2017;482:802-807 pubmed publisher
  8. Roulois D, Deshayes S, Guilly M, Nader J, Liddell C, Robard M, et al. Characterization of preneoplastic and neoplastic rat mesothelial cell lines: the involvement of TETs, DNMTs, and 5-hydroxymethylcytosine. Oncotarget. 2016;7:34664-87 pubmed publisher
  9. Atik Y, Cetinkaya Demir B, Ozan H, Baykara S, Usubutun A, Yılmaz Erturk F. Wilms' tumor 1 protein expression in endometrial adenocarcinoma and endometrial intra-epithelial neoplasia. J Obstet Gynaecol Res. 2016;42:870-5 pubmed publisher
  10. Trembley M, Velasquez L, Small E. Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration. J Vis Exp. 2016;: pubmed publisher
  11. Funk J, Ott V, Herrmann A, Rapp W, Raab S, Riboulet W, et al. Semiautomated quantitative image analysis of glomerular immunohistochemistry markers desmin, vimentin, podocin, synaptopodin and WT-1 in acute and chronic rat kidney disease models. Histochem Cell Biol. 2016;145:315-26 pubmed publisher
  12. Vicente Steijn R, Scherptong R, Kruithof B, Duim S, Goumans M, Wisse L, et al. Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction. PLoS ONE. 2015;10:e0136025 pubmed publisher
  13. Dong H, Chen Z, Wang C, Xiong Z, Zhao W, Jia C, et al. Rictor Regulates Spermatogenesis by Controlling Sertoli Cell Cytoskeletal Organization and Cell Polarity in the Mouse Testis. Endocrinology. 2015;156:4244-56 pubmed publisher
  14. Hu X, Tang Z, Li Y, Liu W, Zhang S, Wang B, et al. Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice. Sci Rep. 2015;5:12982 pubmed publisher
  15. Wang L, Jirka G, Rosenberg P, Buckley A, Gomez J, Fields T, et al. Gq signaling causes glomerular injury by activating TRPC6. J Clin Invest. 2015;125:1913-26 pubmed publisher
  16. Itman C, Bielanowicz A, Goh H, Lee Q, Fulcher A, Moody S, et al. Murine Inhibin α-Subunit Haploinsufficiency Causes Transient Abnormalities in Prepubertal Testis Development Followed by Adult Testicular Decline. Endocrinology. 2015;156:2254-68 pubmed publisher
  17. Shandilya J, Toska E, Richard D, Medler K, Roberts S. WT1 interacts with MAD2 and regulates mitotic checkpoint function. Nat Commun. 2014;5:4903 pubmed publisher
  18. Jeitany M, Pineda J, Liu Q, Porreca R, Hoffschir F, Desmaze C, et al. A preclinical mouse model of glioma with an alternative mechanism of telomere maintenance (ALT). Int J Cancer. 2015;136:1546-58 pubmed publisher
  19. Abdelzaher E, Abdallah D. Expression of mesothelioma-related markers in meningiomas: an immunohistochemical study. Biomed Res Int. 2014;2014:968794 pubmed publisher
  20. Simon L, Ekman G, Garcia T, Carnes K, Zhang Z, Murphy T, et al. ETV5 regulates sertoli cell chemokines involved in mouse stem/progenitor spermatogonia maintenance. Stem Cells. 2010;28:1882-92 pubmed publisher