This is a Validated Antibody Database (VAD) review about mouse Wipi2, based on 17 published articles (read how Labome selects the articles), using Wipi2 antibody in all methods. It is aimed to help Labome visitors find the most suited Wipi2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Wipi2 synonym: 1110018O08Rik; 2510001I10Rik

Abcam
mouse monoclonal (2A2)
  • western blot; mouse; 1:4000; loading ...; fig 5b
Abcam Wipi2 antibody (Abcam, ab105459) was used in western blot on mouse samples at 1:4000 (fig 5b). elife (2019) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; mouse; 1:1200; loading ...; fig 2a
Abcam Wipi2 antibody (Abcam, ab105459) was used in immunocytochemistry on mouse samples at 1:1200 (fig 2a). J Biol Chem (2018) ncbi
mouse monoclonal (2A2)
  • western blot; mouse; loading ...; fig 1m
Abcam Wipi2 antibody (Abcam, ab105459) was used in western blot on mouse samples (fig 1m). Mol Cell (2017) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; human; 1:100; loading ...; fig e2c
In order to research the effect of polyglutamine tracts on the autophagy process, Abcam Wipi2 antibody (Abcam, ab105459) was used in immunocytochemistry on human samples at 1:100 (fig e2c). Nature (2017) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; human; loading ...; fig 1a
  • western blot; human; loading ...; fig 1s1b
Abcam Wipi2 antibody (Abcam, ab105459) was used in immunocytochemistry on human samples (fig 1a) and in western blot on human samples (fig 1s1b). elife (2017) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; human; 1:150; loading ...; fig s3d
In order to recognize the inner mitochondrial membrane protein, prohibitin 2, as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation, Abcam Wipi2 antibody (Abcam, ab105459) was used in immunocytochemistry on human samples at 1:150 (fig s3d). Cell (2017) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; human; 1:500; fig 4c
In order to study the role of PEX13 in autophagy, Abcam Wipi2 antibody (Abcam, ab105459) was used in immunocytochemistry on human samples at 1:500 (fig 4c). EMBO Rep (2017) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; mouse; loading ...; fig 3a
Abcam Wipi2 antibody (Abcam, ab105459) was used in immunocytochemistry on mouse samples (fig 3a). Cell Death Dis (2016) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; rat; fig 1
In order to characterize the ultrastructural relationship of the phagophore and its surrounding organelles, Abcam Wipi2 antibody (Abcam, ab105459) was used in immunocytochemistry on rat samples (fig 1). Autophagy (2015) ncbi
Bio-Rad
mouse monoclonal (2A2)
  • western blot; human; 1:1000; loading ...; fig 2d
Bio-Rad Wipi2 antibody (AbD Serotec, MCA5780GA) was used in western blot on human samples at 1:1000 (fig 2d). Nat Commun (2020) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; human; 1:100; loading ...; fig 1i
Bio-Rad Wipi2 antibody (Bio-Rad, MCA5780GA) was used in immunocytochemistry on human samples at 1:100 (fig 1i). EMBO J (2018) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; human; loading ...; fig s3c
Bio-Rad Wipi2 antibody (AbD Serotec, MCA5780GA) was used in immunocytochemistry on human samples (fig s3c). Dev Cell (2018) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 7f
In order to research the mechanism of LC3 lipidation by modulators of autophagy, Bio-Rad Wipi2 antibody (Bio-Rad, MCA5780GA) was used in immunocytochemistry on mouse samples at 1:100 (fig 7f). Autophagy (2017) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; human; 1:200; fig 3
Bio-Rad Wipi2 antibody (AbD Serotec, MCA5780GA) was used in immunocytochemistry on human samples at 1:200 (fig 3). Nat Commun (2016) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; human; fig 2
Bio-Rad Wipi2 antibody (AbD Serotech, MCA5780GA) was used in immunocytochemistry on human samples (fig 2). Int J Mol Sci (2015) ncbi
mouse monoclonal (2A2)
  • immunocytochemistry; human; 1:200
Bio-Rad Wipi2 antibody (AbD Serotec, MCA5780GA) was used in immunocytochemistry on human samples at 1:200. Curr Protoc Cytom (2014) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig s16a
MilliporeSigma Wipi2 antibody (Sigma, SAB4200400) was used in immunocytochemistry on human samples (fig s16a). Autophagy (2016) ncbi
Articles Reviewed
  1. Boukhalfa A, Nascimbeni A, Ramel D, Dupont N, Hirsch E, Gayral S, et al. PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. Nat Commun. 2020;11:294 pubmed publisher
  2. Stavoe A, Gopal P, Gubas A, Tooze S, Holzbaur E. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons. elife. 2019;8: pubmed publisher
  3. Fletcher K, Ulferts R, Jacquin E, Veith T, Gammoh N, Arasteh J, et al. The WD40 domain of ATG16L1 is required for its non-canonical role in lipidation of LC3 at single membranes. EMBO J. 2018;37: pubmed publisher
  4. Smith M, Harley M, Kemp A, Wills J, Lee M, Arends M, et al. CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis. Dev Cell. 2018;44:217-232.e11 pubmed publisher
  5. Bansal M, Moharir S, Sailasree S, Sirohi K, Sudhakar C, Sarathi D, et al. Optineurin promotes autophagosome formation by recruiting the autophagy-related Atg12-5-16L1 complex to phagophores containing the Wipi2 protein. J Biol Chem. 2018;293:132-147 pubmed publisher
  6. Zhao Y, Chen Y, Miao G, Zhao H, Qu W, Li D, et al. The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. Mol Cell. 2017;67:974-989.e6 pubmed publisher
  7. Ashkenazi A, Bento C, Ricketts T, Vicinanza M, Siddiqi F, Pavel M, et al. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature. 2017;545:108-111 pubmed publisher
  8. Jacquin E, Leclerc Mercier S, Judon C, Blanchard E, Fraitag S, Florey O. Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation. Autophagy. 2017;13:854-867 pubmed publisher
  9. Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch A, Muller S, et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. elife. 2017;6: pubmed publisher
  10. Wei Y, Chiang W, Sumpter R, Mishra P, Levine B. Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor. Cell. 2017;168:224-238.e10 pubmed publisher
  11. Lee M, Sumpter R, Zou Z, Sirasanagandla S, Wei Y, Mishra P, et al. Peroxisomal protein PEX13 functions in selective autophagy. EMBO Rep. 2017;18:48-60 pubmed publisher
  12. Gao Y, Liu Y, Hong L, Yang Z, Cai X, Chen X, et al. Golgi-associated LC3 lipidation requires V-ATPase in noncanonical autophagy. Cell Death Dis. 2016;7:e2330 pubmed publisher
  13. Karanasios E, Walker S, Okkenhaug H, Manifava M, Hummel E, Zimmermann H, et al. Autophagy initiation by ULK complex assembly on ER tubulovesicular regions marked by ATG9 vesicles. Nat Commun. 2016;7:12420 pubmed publisher
  14. Park J, Jung C, Seo M, Otto N, Grunwald D, Kim K, et al. The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14. Autophagy. 2016;12:547-64 pubmed publisher
  15. Macvicar T, Mannack L, Lees R, Lane J. Targeted siRNA Screens Identify ER-to-Mitochondrial Calcium Exchange in Autophagy and Mitophagy Responses in RPE1 Cells. Int J Mol Sci. 2015;16:13356-80 pubmed publisher
  16. Biazik J, Ylä Anttila P, Vihinen H, Jokitalo E, Eskelinen E. Ultrastructural relationship of the phagophore with surrounding organelles. Autophagy. 2015;11:439-51 pubmed publisher
  17. Karanasios E, Stapleton E, Manifava M, Ktistakis N. Imaging autophagy. Curr Protoc Cytom. 2014;69:12.34.1-12.34.16 pubmed publisher