This is a Validated Antibody Database (VAD) review about mouse Wdr5, based on 19 published articles (read how Labome selects the articles), using Wdr5 antibody in all methods. It is aimed to help Labome visitors find the most suited Wdr5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Wdr5 synonym: 2410008O07Rik; AA408785; AA960360; Big; Big-3; WD repeat-containing protein 5; BMP2-induced 3-kb gene protein; WD repeat-containing protein BIG-3

Abcam
rabbit monoclonal (EPR11350)
  • western blot; human; fig 5d
Abcam Wdr5 antibody (Abcam, ab178410) was used in western blot on human samples (fig 5d). Cell (2018) ncbi
mouse monoclonal
  • chromatin immunoprecipitation; human; fig 5
Abcam Wdr5 antibody (Abcam, ab56919) was used in chromatin immunoprecipitation on human samples (fig 5). Mol Cell Biol (2016) ncbi
mouse monoclonal
  • RNA immunoprecipitation; human; fig 3
  • western blot; human; 1:1000; fig 3
Abcam Wdr5 antibody (Abcam, ab56919) was used in RNA immunoprecipitation on human samples (fig 3) and in western blot on human samples at 1:1000 (fig 3). Stem Cell Reports (2015) ncbi
mouse monoclonal
  • RNA immunoprecipitation; human; 1:500; fig s9
  • chromatin immunoprecipitation; human; fig 5
Abcam Wdr5 antibody (Abcam, ab56919) was used in RNA immunoprecipitation on human samples at 1:500 (fig s9) and in chromatin immunoprecipitation on human samples (fig 5). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:2000
Abcam Wdr5 antibody (Abcam, ab22512) was used in western blot on human samples at 1:2000. J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; human
In order to examine the relation between cullin 4B variants and intellectual disability, Abcam Wdr5 antibody (Abcam, ab22512) was used in western blot on human samples . Hum Mutat (2015) ncbi
mouse monoclonal
  • flow cytometry; human
  • western blot; human
Abcam Wdr5 antibody (Abcam, ab56919) was used in flow cytometry on human samples and in western blot on human samples . Oncogene (2015) ncbi
mouse monoclonal
  • chromatin immunoprecipitation; human; fig 3
Abcam Wdr5 antibody (abcam, ab56919) was used in chromatin immunoprecipitation on human samples (fig 3). Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • western blot; human
Abcam Wdr5 antibody (Abacm, ab22512) was used in western blot on human samples . J Biol Chem (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
  • immunoprecipitation; mouse
  • western blot; mouse
Abcam Wdr5 antibody (Abcam, ab22512-100) was used in chromatin immunoprecipitation on mouse samples , in immunoprecipitation on mouse samples and in western blot on mouse samples . EMBO J (2011) ncbi
Bethyl
rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 6a
  • western blot; human; loading ...; fig 1e
Bethyl Wdr5 antibody (Bethyl Laboratories, A302-430A) was used in chromatin immunoprecipitation on human samples (fig 6a) and in western blot on human samples (fig 1e). Biol Open (2016) ncbi
rabbit polyclonal
  • western blot; human; fig s3
Bethyl Wdr5 antibody (Bethyl, A302-430A) was used in western blot on human samples (fig s3). Genome Biol (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4
  • immunoprecipitation; mouse; fig 4
Bethyl Wdr5 antibody (Bethyl Laboratories, A302-430A) was used in western blot on human samples (fig 4) and in immunoprecipitation on mouse samples (fig 4). Mol Cell Proteomics (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig s3
  • western blot; human; fig 2a
In order to test if lncRNA SRA interacts with TrxG or PRC2, Bethyl Wdr5 antibody (Bethyl Laboratories, A302-430A) was used in immunoprecipitation on human samples (fig s3) and in western blot on human samples (fig 2a). PLoS Genet (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 6
Bethyl Wdr5 antibody (Bethyl Laboratories, A302-430A) was used in western blot on human samples (fig 6). Mol Biosyst (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:3500
Bethyl Wdr5 antibody (Bethyl, A302-430A) was used in western blot on human samples at 1:3500. J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; human
Bethyl Wdr5 antibody (Bethyl, A302-430A) was used in western blot on human samples . Oncotarget (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-9)
  • western blot; human; fig 6c
Santa Cruz Biotechnology Wdr5 antibody (Santa Cruz, sc-393080) was used in western blot on human samples (fig 6c). FEBS J (2018) ncbi
Proteintech Group
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100
  • western blot; mouse; 1:2000
Proteintech Group Wdr5 antibody (ProteinTech, 15544-1-AP) was used in immunocytochemistry on mouse samples at 1:100 and in western blot on mouse samples at 1:2000. Proc Natl Acad Sci U S A (2012) ncbi
Articles Reviewed
  1. Hoshii T, Cifani P, Feng Z, Huang C, Koche R, Chen C, et al. A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Cell. 2018;172:1007-1021.e17 pubmed publisher
  2. Bieluszewska A, Weglewska M, Bieluszewski T, Lesniewicz K, Poreba E. PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein. FEBS J. 2018;285:947-964 pubmed publisher
  3. Lu F, Wu X, Yin F, Chia Fang Lee C, Yu M, Mihaylov I, et al. Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases. Biol Open. 2016;5:1449-1460 pubmed publisher
  4. G Hendrickson D, Kelley D, Tenen D, BERNSTEIN B, Rinn J. Widespread RNA binding by chromatin-associated proteins. Genome Biol. 2016;17:28 pubmed publisher
  5. Bode D, Yu L, Tate P, Pardo M, Choudhary J. Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells. Mol Cell Proteomics. 2016;15:878-91 pubmed publisher
  6. Grandy R, Whitfield T, Wu H, Fitzgerald M, VanOudenhove J, Zaidi S, et al. Genome-Wide Studies Reveal that H3K4me3 Modification in Bivalent Genes Is Dynamically Regulated during the Pluripotent Cell Cycle and Stabilized upon Differentiation. Mol Cell Biol. 2016;36:615-27 pubmed publisher
  7. Wongtrakoongate P, Riddick G, Fucharoen S, Felsenfeld G. Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes. PLoS Genet. 2015;11:e1005615 pubmed publisher
  8. Xiao T, Liu L, Li H, Sun Y, Luo H, Li T, et al. Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPα. Stem Cell Reports. 2015;5:856-65 pubmed publisher
  9. Qi D, Wang Q, Li H, Zhang T, Lan R, Kwong D, et al. SILAC-based quantitative proteomics identified lysosome as a fast response target to PDT agent Gd-N induced oxidative stress in human ovarian cancer IGROV1 cells. Mol Biosyst. 2015;11:3059-67 pubmed publisher
  10. Spurlock C, Tossberg J, Guo Y, Collier S, Crooke P, Aune T. Expression and functions of long noncoding RNAs during human T helper cell differentiation. Nat Commun. 2015;6:6932 pubmed publisher
  11. Bailey J, Fields A, Cheng K, Lee A, Wagenaar E, Lagrois R, et al. WD repeat-containing protein 5 (WDR5) localizes to the midbody and regulates abscission. J Biol Chem. 2015;290:8987-9001 pubmed publisher
  12. Shinsky S, Monteith K, Viggiano S, Cosgrove M. Biochemical reconstitution and phylogenetic comparison of human SET1 family core complexes involved in histone methylation. J Biol Chem. 2015;290:6361-75 pubmed publisher
  13. Vulto van Silfhout A, Nakagawa T, Bahi Buisson N, Haas S, Hu H, Bienek M, et al. Variants in CUL4B are associated with cerebral malformations. Hum Mutat. 2015;36:106-17 pubmed publisher
  14. Putiri E, Tiedemann R, Liu C, Choi J, Robertson K. Impact of human MLL/COMPASS and polycomb complexes on the DNA methylome. Oncotarget. 2014;5:6338-52 pubmed
  15. Mungamuri S, Wang S, Manfredi J, Gu W, Aaronson S. Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene. 2015;34:2461-70 pubmed publisher
  16. Ullius A, Lüscher Firzlaff J, Costa I, Walsemann G, Forst A, Gusmao E, et al. The interaction of MYC with the trithorax protein ASH2L promotes gene transcription by regulating H3K27 modification. Nucleic Acids Res. 2014;42:6901-20 pubmed publisher
  17. Zhou P, Wang Z, Yuan X, Zhou C, Liu L, Wan X, et al. Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1). J Biol Chem. 2013;288:17532-43 pubmed publisher
  18. Ma P, Pan H, Montgomery R, Olson E, Schultz R. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 2012;109:E481-9 pubmed publisher
  19. Xie L, Pelz C, Wang W, Bashar A, Varlamova O, Shadle S, et al. KDM5B regulates embryonic stem cell self-renewal and represses cryptic intragenic transcription. EMBO J. 2011;30:1473-84 pubmed publisher