This is a Validated Antibody Database (VAD) review about mouse Upf1, based on 12 published articles (read how Labome selects the articles), using Upf1 antibody in all methods. It is aimed to help Labome visitors find the most suited Upf1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Upf1 synonym: B430202H16Rik; NORF1; PNORF-1; Rent1; Upflp

Santa Cruz Biotechnology
mouse monoclonal (G-3)
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 1d
Santa Cruz Biotechnology Upf1 antibody (Santa Cruz Biotechnology, sc-166092) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 1d). Int J Mol Med (2020) ncbi
mouse monoclonal (G-3)
  • immunoprecipitation; mouse; loading ...; fig 5a
  • immunocytochemistry; mouse; loading ...; fig 5d
Santa Cruz Biotechnology Upf1 antibody (Santa Cruz Biotechnology Inc, sc-166092) was used in immunoprecipitation on mouse samples (fig 5a) and in immunocytochemistry on mouse samples (fig 5d). Proc Natl Acad Sci U S A (2018) ncbi
Abcam
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 7b
  • western blot; human; loading ...; fig 7a
Abcam Upf1 antibody (Abcam, ab86057) was used in immunocytochemistry on human samples (fig 7b) and in western blot on human samples (fig 7a). Mol Cell (2017) ncbi
domestic rabbit monoclonal (EPR4681)
  • western blot; mouse; fig 4
In order to propose that a special mechanism of splicing occurs in the testis and brain whereby H2A.B.3 recruits RNA processing factors from splicing speckles, Abcam Upf1 antibody (Abcam, ab109363) was used in western blot on mouse samples (fig 4). PLoS Genet (2017) ncbi
domestic rabbit monoclonal (EPR4681)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
  • western blot; mouse; fig 1a
Abcam Upf1 antibody (Abcam, ab109363) was used in immunohistochemistry - paraffin section on mouse samples (fig 2a) and in western blot on mouse samples (fig 1a). Sci Rep (2017) ncbi
domestic rabbit monoclonal (EPR4681)
  • western blot; human; loading ...; fig s8d
Abcam Upf1 antibody (Abcam, ab109363) was used in western blot on human samples (fig s8d). BMC Genomics (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D15G6)
  • western blot; human; loading ...; fig 4c
Cell Signaling Technology Upf1 antibody (cst, 12040) was used in western blot on human samples (fig 4c). PLoS ONE (2018) ncbi
domestic rabbit monoclonal (D15G6)
  • western blot; human; loading ...; fig 4c
Cell Signaling Technology Upf1 antibody (Cell signaling, D15G6) was used in western blot on human samples (fig 4c). J Virol (2017) ncbi
domestic rabbit monoclonal (D15G6)
  • western blot; human; fig 4a
In order to identify residues of Y14 that are needed for binding to the mRNA cap, Cell Signaling Technology Upf1 antibody (Cell Signaling Technology, 12040) was used in western blot on human samples (fig 4a). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (D15G6)
  • western blot; human
Cell Signaling Technology Upf1 antibody (Cell Signaling Technology, 12040S) was used in western blot on human samples . Mol Cancer (2015) ncbi
EMD Millipore
domestic rabbit polyclonal
  • western blot; mouse; fig 1b
EMD Millipore Upf1 antibody (Millipore, 07-1016) was used in western blot on mouse samples (fig 1b). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig s5
EMD Millipore Upf1 antibody (Millipore, 07-1016) was used in western blot on human samples at 1:1000 (fig s5). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; mouse; fig 4a
  • western blot; mouse; fig 2c
EMD Millipore Upf1 antibody (Millipore, 07-1014) was used in immunoprecipitation on mouse samples (fig 4a) and in western blot on mouse samples (fig 2c). Nucleic Acids Res (2016) ncbi
Articles Reviewed
  1. Yaojia Cheng Y, Lu Q, Shi N, Zhou Q, Rong J, Li L, et al. Aberrant expression of the UPF1 RNA surveillance gene disturbs keratinocyte homeostasis by stabilizing AREG. Int J Mol Med. 2020;45:1163-1175 pubmed publisher
  2. Cheruiyot A, Li S, Nickless A, Roth R, Fitzpatrick J, You Z. Compound C inhibits nonsense-mediated RNA decay independently of AMPK. PLoS ONE. 2018;13:e0204978 pubmed publisher
  3. Higa M, Oka M, Fujihara Y, Masuda K, Yoneda Y, Kishimoto T. Regulation of inflammatory responses by dynamic subcellular localization of RNA-binding protein Arid5a. Proc Natl Acad Sci U S A. 2018;115:E1214-E1220 pubmed publisher
  4. Feng Q, Jagannathan S, Bradley R. The RNA Surveillance Factor UPF1 Represses Myogenesis via Its E3 Ubiquitin Ligase Activity. Mol Cell. 2017;67:239-251.e6 pubmed publisher
  5. Soboleva T, Parker B, Nekrasov M, Hart Smith G, Tay Y, Tng W, et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet. 2017;13:e1006633 pubmed publisher
  6. Mooney C, Jimenez Mateos E, Engel T, Mooney C, Diviney M, Venø M, et al. RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis. Sci Rep. 2017;7:41517 pubmed publisher
  7. Balinsky C, Schmeisser H, Wells A, Ganesan S, Jin T, Singh K, et al. IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10. J Virol. 2017;91: pubmed publisher
  8. Durand S, Franks T, Lykke Andersen J. Hyperphosphorylation amplifies UPF1 activity to resolve stalls in nonsense-mediated mRNA decay. Nat Commun. 2016;7:12434 pubmed publisher
  9. Chuang T, Lee K, Lou Y, Lu C, Tarn W. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J Biol Chem. 2016;291:8565-74 pubmed publisher
  10. Sanchez G, Bondy Chorney E, Laframboise J, Paris G, Didillon A, Jasmin B, et al. A novel role for CARM1 in promoting nonsense-mediated mRNA decay: potential implications for spinal muscular atrophy. Nucleic Acids Res. 2016;44:2661-76 pubmed publisher
  11. He X, Zhang P. Serine/arginine-rich splicing factor 3 (SRSF3) regulates homologous recombination-mediated DNA repair. Mol Cancer. 2015;14:158 pubmed publisher
  12. Maekawa S, Imamachi N, Irie T, Tani H, Matsumoto K, Mizutani R, et al. Analysis of RNA decay factor mediated RNA stability contributions on RNA abundance. BMC Genomics. 2015;16:154 pubmed publisher