This is a Validated Antibody Database (VAD) review about mouse Ube2n, based on 20 published articles (read how Labome selects the articles), using Ube2n antibody in all methods. It is aimed to help Labome visitors find the most suited Ube2n antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ube2n synonym: 1500026J17Rik; AL022654; BB101821; UBC13; ubiquitin-conjugating enzyme E2 N; E2 ubiquitin-conjugating enzyme N; bendless-like ubiquitin-conjugating enzyme; ubiquitin carrier protein N; ubiquitin-protein ligase N

Knockout validation
Invitrogen
mouse monoclonal (4E 11)
  • immunoprecipitation; human; fig 3
  • western blot; human
  • western blot knockout validation; mouse; fig 5
In order to study the requirement of ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation by human T-cell leukemia virus 1 tax oncoprotein, Invitrogen Ube2n antibody (Invitrogen/Zymed, 4E11) was used in immunoprecipitation on human samples (fig 3), in western blot on human samples and in western blot knockout validation on mouse samples (fig 5). J Virol (2007) ncbi
EMD Millipore
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 8a
  • western blot; human; loading ...; fig 5a, 7a
EMD Millipore Ube2n antibody (EMD Millipore, Ab-1) was used in western blot knockout validation on mouse samples (fig 8a) and in western blot on human samples (fig 5a, 7a). Oncogene (2017) ncbi
Invitrogen
mouse monoclonal (4E 11)
  • western blot; human; loading ...; fig 3c
In order to investigate the involvement of RNF8- and Ube2S-dependent ubiquitin lysine 11-linkage modification in the DNA damage response, Invitrogen Ube2n antibody (Zymed Laboratories, 37-1100) was used in western blot on human samples (fig 3c). Mol Cell (2017) ncbi
mouse monoclonal (4E 11)
  • western blot; human; loading ...; fig 3c
In order to study the activation of TAK1 by interleukin-1 and TRAF6, Invitrogen Ube2n antibody (Invitrogen, 37-1100) was used in western blot on human samples (fig 3c). Biochem J (2017) ncbi
mouse monoclonal (4E 11)
  • western blot; mouse; loading ...; fig 1c
In order to discover that Tax recruits linear (Met1-linked) ubiquitin chain assembly complex to the IkB kinase complex, Invitrogen Ube2n antibody (ThermoFisher, 37-1100) was used in western blot on mouse samples (fig 1c). PLoS Pathog (2017) ncbi
mouse monoclonal (4E 11)
  • western blot; human; 1:1000; loading ...; fig 3c
In order to find inhibitors of the RNF8-Ubc13 interaction, Invitrogen Ube2n antibody (Invitrogen, 37-1100) was used in western blot on human samples at 1:1000 (fig 3c). SLAS Discov (2017) ncbi
mouse monoclonal (4E 11)
  • western blot; mouse; 1:1000; fig 1g
In order to elucidate the mechanism by which STAT3 controls NF-kapaB signaling in innate immune cells, Invitrogen Ube2n antibody (Invitrogen, 371100) was used in western blot on mouse samples at 1:1000 (fig 1g). Nat Commun (2014) ncbi
mouse monoclonal (4E 11)
  • western blot; mouse; 1:1,000
In order to examine the involvement of lysine 63-linked polyubiquitin during Parkin-mediated mitophagy, Invitrogen Ube2n antibody (Life Technologies, 4E11) was used in western blot on mouse samples at 1:1,000. J Biol Chem (2014) ncbi
mouse monoclonal (4E 11)
  • western blot; human; 1:1000; fig s1
In order to identify E2s that regulate the activation, translocation, and enzymatic functions of Parkin during mitochondrial quality control, Invitrogen Ube2n antibody (Invitrogen, 37-1100) was used in western blot on human samples at 1:1000 (fig s1). J Cell Sci (2014) ncbi
mouse monoclonal (4E 11)
  • western blot; mouse
In order to report a role for USP21 in the negative regulation of antiviral response through deubiquitinating RIG-I, Invitrogen Ube2n antibody (Invitrogen, 371100) was used in western blot on mouse samples . J Exp Med (2014) ncbi
mouse monoclonal (4E 11)
  • western blot; human; 1:1000; fig 5
In order to assess the deubiquitinating activity of OTUB1 regulated by E2 ubiquitin-conjugating enzymes, Invitrogen Ube2n antibody (Life Technologies, 37-1100) was used in western blot on human samples at 1:1000 (fig 5). Nat Struct Mol Biol (2013) ncbi
mouse monoclonal (4E 11)
  • western blot; human; fig 8
In order to identify and characterize compounds that inhibit NF-kappaB activation in diffuse large B-cell lymphoma cells, Invitrogen Ube2n antibody (Invitrogen, 37-1100) was used in western blot on human samples (fig 8). Blood (2012) ncbi
mouse monoclonal (4E 11)
  • western blot; human; 1:1000; fig s4
In order to study the role of ZRANB3 translocase during replication, Invitrogen Ube2n antibody (Invitrogen, 37-1100) was used in western blot on human samples at 1:1000 (fig s4). Mol Cell (2012) ncbi
mouse monoclonal (4E 11)
  • immunoprecipitation; human
  • western blot; human
In order to show that RNF168 is mutated in individuals with RIDDLE syndrome, Invitrogen Ube2n antibody (Zymed, 4E11) was used in immunoprecipitation on human samples and in western blot on human samples . Cell (2009) ncbi
mouse monoclonal (4E 11)
  • western blot; human
In order to investigate the potential role of the proteasome in checkpoint activation and ATM/ATR signaling in response to UV light-induced DNA damage, Invitrogen Ube2n antibody (Zymed Laboratories Inc, 4E11) was used in western blot on human samples . J Biol Chem (2008) ncbi
mouse monoclonal (4E 11)
  • immunoprecipitation; human; fig 3
  • western blot; human
  • western blot knockout validation; mouse; fig 5
In order to study the requirement of ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation by human T-cell leukemia virus 1 tax oncoprotein, Invitrogen Ube2n antibody (Invitrogen/Zymed, 4E11) was used in immunoprecipitation on human samples (fig 3), in western blot on human samples and in western blot knockout validation on mouse samples (fig 5). J Virol (2007) ncbi
mouse monoclonal (4E 11)
  • western blot; mouse; fig 1
In order to determine the function of Ubc13 in T cell receptor-mediated signaling and responses, Invitrogen Ube2n antibody (ZYMED, 37-1100) was used in western blot on mouse samples (fig 1). J Immunol (2006) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-10)
  • western blot; mouse; 1:200; loading ...; fig 6b
In order to research the effect of anti-inflammatory factor A20 on cerebral inflammatory injury, Santa Cruz Biotechnology Ube2n antibody (SantaCruz, sc-376470) was used in western blot on mouse samples at 1:200 (fig 6b). J Immunol (2017) ncbi
Bio-Rad
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 7b
Bio-Rad Ube2n antibody (Bio-Rad, AHP974) was used in western blot on mouse samples at 1:1000 (fig 7b). elife (2018) ncbi
Cell Signaling Technology
rabbit monoclonal (D2A1)
  • western blot; human; 1:5000; fig s1
In order to identify E2s that regulate the activation, translocation, and enzymatic functions of Parkin during mitochondrial quality control, Cell Signaling Technology Ube2n antibody (Cell Signaling Technology, 6999) was used in western blot on human samples at 1:5000 (fig s1). J Cell Sci (2014) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; loading ...; fig 2f
EMD Millipore Ube2n antibody (Calbiochem, Ab-1) was used in western blot on human samples (fig 2f). Oncogene (2017) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 8a
  • western blot; human; loading ...; fig 5a, 7a
EMD Millipore Ube2n antibody (EMD Millipore, Ab-1) was used in western blot knockout validation on mouse samples (fig 8a) and in western blot on human samples (fig 5a, 7a). Oncogene (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig s8
EMD Millipore Ube2n antibody (Calbiochem, Ab1) was used in western blot on human samples (fig s8). Science (2016) ncbi
Articles Reviewed
  1. Dickson C, Fletcher A, Vaysburd M, Yang J, Mallery D, Zeng J, et al. Intracellular antibody signalling is regulated by phosphorylation of the Fc receptor TRIM21. elife. 2018;7: pubmed publisher
  2. Giono L, Resnick Silverman L, Carvajal L, St Clair S, Manfredi J. Mdm2 promotes Cdc25C protein degradation and delays cell cycle progression through the G2/M phase. Oncogene. 2017;36:6762-6773 pubmed publisher
  3. Senturk J, Bohlman S, Manfredi J. Mdm2 selectively suppresses DNA damage arising from inhibition of topoisomerase II independent of p53. Oncogene. 2017;36:6085-6096 pubmed publisher
  4. Paul A, Wang B. RNF8- and Ube2S-Dependent Ubiquitin Lysine 11-Linkage Modification in Response to DNA Damage. Mol Cell. 2017;66:458-472.e5 pubmed publisher
  5. Zhang J, MacArtney T, Peggie M, Cohen P. Interleukin-1 and TRAF6-dependent activation of TAK1 in the absence of TAB2 and TAB3. Biochem J. 2017;474:2235-2248 pubmed publisher
  6. Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, et al. HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains. PLoS Pathog. 2017;13:e1006162 pubmed publisher
  7. Meng Z, Zhao T, Zhou K, Zhong Q, Wang Y, Xiong X, et al. A20 Ameliorates Intracerebral Hemorrhage-Induced Inflammatory Injury by Regulating TRAF6 Polyubiquitination. J Immunol. 2017;198:820-831 pubmed publisher
  8. Weber E, Rothenaigner I, Brandner S, Hadian K, Schorpp K. A High-Throughput Screening Strategy for Development of RNF8-Ubc13 Protein-Protein Interaction Inhibitors. SLAS Discov. 2017;22:316-323 pubmed publisher
  9. Chen S, FORRESTER W, Lahav G. Schedule-dependent interaction between anticancer treatments. Science. 2016;351:1204-8 pubmed publisher
  10. Zhang H, Hu H, Greeley N, Jin J, Matthews A, Ohashi E, et al. STAT3 restrains RANK- and TLR4-mediated signalling by suppressing expression of the E2 ubiquitin-conjugating enzyme Ubc13. Nat Commun. 2014;5:5798 pubmed publisher
  11. Shiba Fukushima K, Inoshita T, Hattori N, Imai Y. Lysine 63-linked polyubiquitination is dispensable for Parkin-mediated mitophagy. J Biol Chem. 2014;289:33131-6 pubmed publisher
  12. Fiesel F, Moussaud Lamodière E, Ando M, Springer W. A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently. J Cell Sci. 2014;127:3488-504 pubmed publisher
  13. Fan Y, Mao R, Yu Y, Liu S, Shi Z, Cheng J, et al. USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase. J Exp Med. 2014;211:313-28 pubmed publisher
  14. Wiener R, Dibello A, Lombardi P, Guzzo C, Zhang X, Matunis M, et al. E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nat Struct Mol Biol. 2013;20:1033-9 pubmed publisher
  15. Pulvino M, Liang Y, Oleksyn D, DeRan M, Van Pelt E, Shapiro J, et al. Inhibition of proliferation and survival of diffuse large B-cell lymphoma cells by a small-molecule inhibitor of the ubiquitin-conjugating enzyme Ubc13-Uev1A. Blood. 2012;120:1668-77 pubmed publisher
  16. Ciccia A, Nimonkar A, Hu Y, Hajdu I, Achar Y, Izhar L, et al. Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress. Mol Cell. 2012;47:396-409 pubmed publisher
  17. Stewart G, Panier S, Townsend K, Al Hakim A, Kolas N, Miller E, et al. The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage. Cell. 2009;136:420-34 pubmed publisher
  18. Sakasai R, Tibbetts R. RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage. J Biol Chem. 2008;283:13549-55 pubmed publisher
  19. Shembade N, Harhaj N, Yamamoto M, Akira S, Harhaj E. The human T-cell leukemia virus type 1 Tax oncoprotein requires the ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation. J Virol. 2007;81:13735-42 pubmed
  20. Yamamoto M, Sato S, Saitoh T, Sakurai H, Uematsu S, Kawai T, et al. Cutting Edge: Pivotal function of Ubc13 in thymocyte TCR signaling. J Immunol. 2006;177:7520-4 pubmed