This is a Validated Antibody Database (VAD) review about mouse Top2a, based on 9 published articles (read how Labome selects the articles), using Top2a antibody in all methods. It is aimed to help Labome visitors find the most suited Top2a antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Top2a synonym: Top-2

Abcam
domestic rabbit monoclonal (EP1102Y)
  • western blot; human; 1:1000; loading ...; fig 5d
Abcam Top2a antibody (Abcam, ab52934) was used in western blot on human samples at 1:1000 (fig 5d). J Exp Clin Cancer Res (2021) ncbi
domestic rabbit monoclonal (EP1102Y)
  • ChIP-Seq; mouse; ; loading ...; fig 4a
Abcam Top2a antibody (Abcam, EP1102Y) was used in ChIP-Seq on mouse samples at (fig 4a). Nat Commun (2020) ncbi
domestic rabbit monoclonal (EP1102Y)
  • western blot; human; loading ...; fig s10c
Abcam Top2a antibody (Abcam, EP1102Y) was used in western blot on human samples (fig s10c). Oncogene (2017) ncbi
domestic rabbit monoclonal (EP1102Y)
  • immunoprecipitation; mouse; loading ...; fig 7d
  • western blot; mouse; 1:3000; loading ...; fig 6a
  • western blot; human; 1:3000; loading ...; fig 6b
Abcam Top2a antibody (Abcam, ab52934) was used in immunoprecipitation on mouse samples (fig 7d), in western blot on mouse samples at 1:3000 (fig 6a) and in western blot on human samples at 1:3000 (fig 6b). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EP1102Y)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 8c
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 8a
In order to address the effects of etoposide on germ cells in the developing ovary, Abcam Top2a antibody (Abcam, ab52934) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 8c) and in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 8a). BMC Cancer (2016) ncbi
domestic rabbit monoclonal (EP1102Y)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 1b
  • western blot; mouse; loading ...; fig 1e
In order to examine the role of topoisomerase II in mitotic clonal expansion and adipogenesis, Abcam Top2a antibody (Abcam, ab52934) was used in immunocytochemistry on mouse samples at 1:100 (fig 1b) and in western blot on mouse samples (fig 1e). Cell Signal (2016) ncbi
domestic rabbit monoclonal (EPR5377)
  • western blot; human; 1:1000; loading ...; fig 3a
Abcam Top2a antibody (Abcam, ab109524) was used in western blot on human samples at 1:1000 (fig 3a). Oncotarget (2016) ncbi
domestic rabbit monoclonal (EP1102Y)
  • chromatin immunoprecipitation; mouse
Abcam Top2a antibody (Abcam, ab52934) was used in chromatin immunoprecipitation on mouse samples . Cell Cycle (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-12)
  • immunohistochemistry; mouse; fig 3
  • western blot; mouse; fig 3
In order to study the roles of Top2-alpha and -beta in mitochondria and the effect on mitochondrial DNA supercoiling of Top1mt deletion, Santa Cruz Biotechnology Top2a antibody (Santa Cruz, sc-365916) was used in immunohistochemistry on mouse samples (fig 3) and in western blot on mouse samples (fig 3). Nucleic Acids Res (2014) ncbi
Articles Reviewed
  1. Lei X, Cao K, Chen Y, Shen H, Liu Z, Qin H, et al. Nuclear Transglutaminase 2 interacts with topoisomerase II⍺ to promote DNA damage repair in lung cancer cells. J Exp Clin Cancer Res. 2021;40:224 pubmed publisher
  2. Alvarez Quilón A, Terron Bautista J, Delgado Sainz I, Serrano Benítez A, Romero Granados R, Martínez García P, et al. Endogenous topoisomerase II-mediated DNA breaks drive thymic cancer predisposition linked to ATM deficiency. Nat Commun. 2020;11:910 pubmed publisher
  3. Senturk J, Bohlman S, Manfredi J. Mdm2 selectively suppresses DNA damage arising from inhibition of topoisomerase II independent of p53. Oncogene. 2017;36:6085-6096 pubmed publisher
  4. Guturi K, Bohgaki M, Bohgaki T, Srikumar T, Ng D, Kumareswaran R, et al. RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation. Nat Commun. 2016;7:12638 pubmed publisher
  5. Stefansdottir A, Johnston Z, Powles Glover N, Anderson R, Adams I, Spears N. Etoposide damages female germ cells in the developing ovary. BMC Cancer. 2016;16:482 pubmed publisher
  6. Jacobsen R, Mazloumi Gavgani F, Mellgren G, Lewis A. DNA Topoisomerase II? contributes to the early steps of adipogenesis in 3T3-L1 cells. Cell Signal. 2016;28:1593-603 pubmed publisher
  7. Morris J, Moseley V, Cabang A, Coleman K, Wei W, Garrett Mayer E, et al. Reduction in promotor methylation utilizing EGCG (epigallocatechin-3-gallate) restores RXR? expression in human colon cancer cells. Oncotarget. 2016;7:35313-26 pubmed publisher
  8. Zhang H, Zhang Y, Yasukawa T, Dalla Rosa I, Khiati S, Pommier Y. Increased negative supercoiling of mtDNA in TOP1mt knockout mice and presence of topoisomerases II? and II? in vertebrate mitochondria. Nucleic Acids Res. 2014;42:7259-67 pubmed publisher
  9. d Alcontres M, Palacios J, Mejias D, Blasco M. TopoII? prevents telomere fragility and formation of ultra thin DNA bridges during mitosis through TRF1-dependent binding to telomeres. Cell Cycle. 2014;13:1463-81 pubmed publisher