This is a Validated Antibody Database (VAD) review about mouse Top1, based on 7 published articles (read how Labome selects the articles), using Top1 antibody in all methods. It is aimed to help Labome visitors find the most suited Top1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Top1 synonym: AI467334; D130064I21Rik; Top-1

Knockout validation
Abcam
domestic rabbit monoclonal (EPR5375)
  • immunocytochemistry; mouse; fig 4d
  • western blot knockout validation; human; loading ...; fig s5f
Abcam Top1 antibody (Abcam, ab109374) was used in immunocytochemistry on mouse samples (fig 4d) and in western blot knockout validation on human samples (fig s5f). Mol Cancer (2021) ncbi
Abcam
domestic rabbit monoclonal (EPR5375)
  • immunocytochemistry; mouse; fig 4d
  • western blot knockout validation; human; loading ...; fig s5f
Abcam Top1 antibody (Abcam, ab109374) was used in immunocytochemistry on mouse samples (fig 4d) and in western blot knockout validation on human samples (fig s5f). Mol Cancer (2021) ncbi
domestic rabbit monoclonal (EPR5375)
  • western blot; human; 1:5000; loading ...; fig s6c
Abcam Top1 antibody (Abcam, ab109374) was used in western blot on human samples at 1:5000 (fig s6c). Nature (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1b
Abcam Top1 antibody (Abcam, ab3825) was used in western blot on human samples (fig 1b). Nucleic Acids Res (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:400; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Abcam Top1 antibody (Abcam, ab85038) was used in western blot on human samples at 1:400 (fig st1). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EPR5375)
  • immunoprecipitation; human; fig 4
Abcam Top1 antibody (Abcam, ab109374) was used in immunoprecipitation on human samples (fig 4). Cell Rep (2016) ncbi
domestic rabbit monoclonal (EPR5375)
  • western blot; human; fig 6
Abcam Top1 antibody (Abcam, ab109374) was used in western blot on human samples (fig 6). Nucleic Acids Res (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-5)
  • western blot; mouse; 1:250; fig s1
Santa Cruz Biotechnology Top1 antibody (Santa Cruz, sc-271285) was used in western blot on mouse samples at 1:250 (fig s1). PLoS ONE (2016) ncbi
Articles Reviewed
  1. Gu Y, Wang Y, He L, Zhang J, Zhu X, Liu N, et al. Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. Mol Cancer. 2021;20:132 pubmed publisher
  2. Zimmermann M, Murina O, Reijns M, Agathanggelou A, Challis R, Tarnauskaitė Ž, et al. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature. 2018;559:285-289 pubmed publisher
  3. Ahmad M, Shen W, Li W, Xue Y, Zou S, Xu D, et al. Topoisomerase 3? is the major topoisomerase for mRNAs and linked to neurodevelopment and mental dysfunction. Nucleic Acids Res. 2017;45:2704-2713 pubmed publisher
  4. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  5. Mabb A, Simon J, KING I, Lee H, An L, Philpot B, et al. Topoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms. PLoS ONE. 2016;11:e0156439 pubmed publisher
  6. Ribeyre C, Zellweger R, Chauvin M, Bec N, Larroque C, Lopes M, et al. Nascent DNA Proteomics Reveals a Chromatin Remodeler Required for Topoisomerase I Loading at Replication Forks. Cell Rep. 2016;15:300-9 pubmed publisher
  7. Cristini A, Park J, Capranico G, Legube G, Favre G, Sordet O. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2016;44:1161-78 pubmed publisher