This is a Validated Antibody Database (VAD) review about mouse Themis, based on 3 published articles (read how Labome selects the articles), using Themis antibody in all methods. It is aimed to help Labome visitors find the most suited Themis antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Themis synonym: E430004N04Rik; Gasp; Spot; Tsepa; thylex; protein THEMIS; grb2-associating protein; signaling phosphoprotein specific for T cells; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; thymus lymphocyte exclusively expressed

Knockout validation
EMD Millipore
rabbit polyclonal
  • western blot knockout validation; mouse; fig 3
  • immunoprecipitation; mouse; fig 3
In order to describe the role of Themis in T cell development, EMD Millipore Themis antibody (Millipore, 06-1328) was used in western blot knockout validation on mouse samples (fig 3) and in immunoprecipitation on mouse samples (fig 3). Nature (2013) ncbi
Abcam
rabbit monoclonal (EPR7353)
  • immunocytochemistry; human; loading ...; fig 8a
In order to assess whether human T-cell leukemia virus type 1 bZIP factor enhances the proliferation of expressing T cells after stimulation via the T-cell receptor, Abcam Themis antibody (Abcam, ab126771) was used in immunocytochemistry on human samples (fig 8a). PLoS Pathog (2017) ncbi
EMD Millipore
rabbit polyclonal
  • immunoprecipitation; mouse
  • immunocytochemistry; mouse
EMD Millipore Themis antibody (Millipore, 06-1328) was used in immunoprecipitation on mouse samples and in immunocytochemistry on mouse samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; fig 3
  • immunoprecipitation; mouse; fig 3
In order to describe the role of Themis in T cell development, EMD Millipore Themis antibody (Millipore, 06-1328) was used in western blot knockout validation on mouse samples (fig 3) and in immunoprecipitation on mouse samples (fig 3). Nature (2013) ncbi
Articles Reviewed
  1. Kinosada H, Yasunaga J, Shimura K, Miyazato P, Onishi C, Iyoda T, et al. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS Pathog. 2017;13:e1006120 pubmed publisher
  2. Okada T, Nitta T, Kaji K, Takashima A, Oda H, Tamehiro N, et al. Differential function of Themis CABIT domains during T cell development. PLoS ONE. 2014;9:e89115 pubmed publisher
  3. Fu G, Casas J, Rigaud S, Rybakin V, Lambolez F, Brzostek J, et al. Themis sets the signal threshold for positive and negative selection in T-cell development. Nature. 2013;504:441-5 pubmed publisher