This is a Validated Antibody Database (VAD) review about mouse Tek, based on 23 published articles (read how Labome selects the articles), using Tek antibody in all methods. It is aimed to help Labome visitors find the most suited Tek antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Tek synonym: AA517024; Cd202b; Hyk; STK1; Tie-2; Tie2

Knockout validation
R&D Systems
domestic goat polyclonal
  • immunohistochemistry - frozen section; human; loading ...; fig 2a
  • immunohistochemistry knockout validation; mouse; loading ...; fig s1b
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
R&D Systems Tek antibody (R&D Systems, AF762) was used in immunohistochemistry - frozen section on human samples (fig 2a), in immunohistochemistry knockout validation on mouse samples (fig s1b) and in immunohistochemistry - frozen section on mouse samples (fig 1a). J Clin Invest (2017) ncbi
Invitrogen
rat monoclonal (TEK4)
  • flow cytometry; mouse; 1:20; fig 2d
Invitrogen Tek antibody (ThermoFisher, 14-5987) was used in flow cytometry on mouse samples at 1:20 (fig 2d). elife (2021) ncbi
rat monoclonal (TEK4)
  • flow cytometry; mouse; 1:200; fig s6a
Invitrogen Tek antibody (eBioscience, 13-5987-81) was used in flow cytometry on mouse samples at 1:200 (fig s6a). Nat Commun (2020) ncbi
rat monoclonal (TEK4)
  • flow cytometry; mouse; loading ...; fig s3c
Invitrogen Tek antibody (ebioscience, 12-5987) was used in flow cytometry on mouse samples (fig s3c). Epigenetics Chromatin (2019) ncbi
rat monoclonal (TEK4)
  • flow cytometry; mouse; loading ...; fig 2B
In order to investigate the role of endoglin in the development of hematopoietic cells., Invitrogen Tek antibody (e-biosciences, 13-5987) was used in flow cytometry on mouse samples (fig 2B). Biol Open (2015) ncbi
rat monoclonal (TEK4)
  • immunohistochemistry; mouse; 1:600; fig 8
Invitrogen Tek antibody (eBiosciences, 14-5987-82) was used in immunohistochemistry on mouse samples at 1:600 (fig 8). J Biol Chem (2015) ncbi
rat monoclonal (TEK4)
  • flow cytometry; mouse
Invitrogen Tek antibody (eBiosciences, TEK4) was used in flow cytometry on mouse samples . PLoS ONE (2014) ncbi
R&D Systems
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5e
R&D Systems Tek antibody (R&D Systems, AF2720) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 5e). Nat Neurosci (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s2a
R&D Systems Tek antibody (R&D Systems, AF2720) was used in western blot on human samples (fig s2a). J Cell Biol (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
R&D Systems Tek antibody (R&D Systems, AF2720) was used in immunohistochemistry - frozen section on mouse samples (fig 1a). J Clin Invest (2017) ncbi
domestic goat polyclonal
  • immunohistochemistry - frozen section; human; loading ...; fig 2a
  • immunohistochemistry knockout validation; mouse; loading ...; fig s1b
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
R&D Systems Tek antibody (R&D Systems, AF762) was used in immunohistochemistry - frozen section on human samples (fig 2a), in immunohistochemistry knockout validation on mouse samples (fig s1b) and in immunohistochemistry - frozen section on mouse samples (fig 1a). J Clin Invest (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 2c
  • western blot; human; loading ...; fig 2d
R&D Systems Tek antibody (R&D Systems, AF2720) was used in immunocytochemistry on human samples (fig 2c) and in western blot on human samples (fig 2d). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:100; loading ...; fig 2h
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5f
In order to study the role of endothelial cell exocytosis in cerebral cavernous malformation disease progression, R&D Systems Tek antibody (R&D, AF2720) was used in immunohistochemistry - frozen section on human samples at 1:100 (fig 2h) and in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 5f). Nat Med (2016) ncbi
domestic goat polyclonal
  • immunohistochemistry - free floating section; mouse; 1:50; fig 9
  • western blot; mouse; 1:500; fig 8
In order to determine the rescue of VE-PTP-dependent restoration of Tie2 signaling from angiopoietin-2-induced blood-brain barrier compromise and increased stroke size, R&D Systems Tek antibody (R&D Systems, AF762) was used in immunohistochemistry - free floating section on mouse samples at 1:50 (fig 9) and in western blot on mouse samples at 1:500 (fig 8). Acta Neuropathol (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:50; fig 9
  • western blot; mouse; 1:400; fig 8
In order to determine the rescue of VE-PTP-dependent restoration of Tie2 signaling from angiopoietin-2-induced blood-brain barrier compromise and increased stroke size, R&D Systems Tek antibody (R&D Systems, AF2720) was used in immunohistochemistry - free floating section on mouse samples at 1:50 (fig 9) and in western blot on mouse samples at 1:400 (fig 8). Acta Neuropathol (2016) ncbi
domestic goat polyclonal
In order to determine the roles of Sox7, Sox17, and Sox18 in the developing and mature mouse vasculature, R&D Systems Tek antibody (R&D Systems, AF762) was used . PLoS ONE (2015) ncbi
Abcam
mouse monoclonal (Cl. 16)
  • immunohistochemistry - frozen section; mouse; 1:150; loading ...; fig 1b, s1e, s4b
Abcam Tek antibody (Abcam, ab24859) was used in immunohistochemistry - frozen section on mouse samples at 1:150 (fig 1b, s1e, s4b). Sci Adv (2021) ncbi
mouse monoclonal (Cl. 16)
  • western blot; human; loading ...; fig 4a
Abcam Tek antibody (Abcam, ab24859) was used in western blot on human samples (fig 4a). Lab Invest (2017) ncbi
mouse monoclonal (Cl. 16)
  • flow cytometry; mouse; fig s2b
  • immunocytochemistry; mouse; 1:500; fig st1
In order to discuss the cellular origins of fibroadipocytes in arrhythmogenic cardiomyopathy, Abcam Tek antibody (Abcam, 24859) was used in flow cytometry on mouse samples (fig s2b) and in immunocytochemistry on mouse samples at 1:500 (fig st1). Circ Res (2016) ncbi
mouse monoclonal (Cl. 16)
  • western blot; human
Abcam Tek antibody (Abcam, ab24859) was used in western blot on human samples . Mol Syndromol (2013) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; fig 7
Novus Biologicals Tek antibody (Novus Biologicals, NBP 1-69753) was used in immunohistochemistry on mouse samples at 1:200 (fig 7). Am J Transl Res (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; fig 6c
Cell Signaling Technology Tek antibody (Cell Signaling Technology, 4221S) was used in western blot on human samples (fig 6c). Angiogenesis (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s11b
Cell Signaling Technology Tek antibody (Cell Signaling, 4221) was used in western blot on mouse samples at 1:1000 (fig s11b). Nat Commun (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 5f
Cell Signaling Technology Tek antibody (Cell Signaling, 4221) was used in western blot on human samples (fig 5f). Breast Cancer Res (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig s4h
  • western blot; mouse; 1:1000; loading ...; fig 5i
In order to study the role of endothelial cell exocytosis in cerebral cavernous malformation disease progression, Cell Signaling Technology Tek antibody (Cell Signaling, 4221) was used in western blot on human samples at 1:1000 (fig s4h) and in western blot on mouse samples at 1:1000 (fig 5i). Nat Med (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 4
Cell Signaling Technology Tek antibody (Cell Signaling, 4221) was used in western blot on human samples (fig 4). Endocr Relat Cancer (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3a
Cell Signaling Technology Tek antibody (Cell Signaling, 4221) was used in western blot on human samples (fig 3a). PLoS ONE (2016) ncbi
BD Biosciences
mouse monoclonal (33)
  • immunohistochemistry; mouse
BD Biosciences Tek antibody (BD Pharmingen, 557039) was used in immunohistochemistry on mouse samples . PLoS ONE (2014) ncbi
Articles Reviewed
  1. Ambrosi T, Sinha R, Steininger H, Hoover M, Murphy M, Koepke L, et al. Distinct skeletal stem cell types orchestrate long bone skeletogenesis. elife. 2021;10: pubmed publisher
  2. Baganha F, de Jong R, Peters E, Voorham W, Jukema J, Delibegovic M, et al. Atorvastatin pleiotropically decreases intraplaque angiogenesis and intraplaque haemorrhage by inhibiting ANGPT2 release and VE-Cadherin internalization. Angiogenesis. 2021;24:567-581 pubmed publisher
  3. Chen J, Sivan U, Tan S, Lippo L, De Angelis J, Labella R, et al. High-resolution 3D imaging uncovers organ-specific vascular control of tissue aging. Sci Adv. 2021;7: pubmed publisher
  4. Paredes I, Vieira J, Shah B, Ramunno C, Dyckow J, Adler H, et al. Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk. Nat Neurosci. 2021;24:478-488 pubmed publisher
  5. Zhou H, Qin L, Jiang Q, Murray K, Zhang H, Li B, et al. Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model. Nat Commun. 2021;12:504 pubmed publisher
  6. Kim J, Yang Y, Park K, Ge X, Xu R, Li N, et al. A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation. Nat Commun. 2020;11:2289 pubmed publisher
  7. Gilmour J, O Connor L, Middleton C, Keane P, Gillemans N, Cazier J, et al. Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors. Epigenetics Chromatin. 2019;12:33 pubmed publisher
  8. Juettner V, Kruse K, Dan A, Vu V, Khan Y, Le J, et al. VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism. J Cell Biol. 2019;218:1725-1742 pubmed publisher
  9. Kennedy S, Han J, Portman N, Nobis M, Hastings J, Murphy K, et al. Targeting promiscuous heterodimerization overcomes innate resistance to ERBB2 dimerization inhibitors in breast cancer. Breast Cancer Res. 2019;21:43 pubmed publisher
  10. Kim J, Park D, Bae H, Park D, Kim D, Lee C, et al. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma. J Clin Invest. 2017;127:3877-3896 pubmed publisher
  11. Leppänen V, Saharinen P, Alitalo K. Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization. Proc Natl Acad Sci U S A. 2017;114:4376-4381 pubmed publisher
  12. Wang K, Liu W, Song Y, Wu X, Zhang Y, Li S, et al. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration. Lab Invest. 2017;97:971-982 pubmed publisher
  13. Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, et al. Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Med. 2016;22:1033-1042 pubmed publisher
  14. Han H, Kim B, Lee J, Kang S, Kim J, Cho N. Angiopoietin-2 promotes ER+ breast cancer cell survival in bone marrow niche. Endocr Relat Cancer. 2016;23:609-23 pubmed publisher
  15. Sun L, Zhang H, Bi L, Shi Y, Xing C, Tang L, et al. Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice. Am J Transl Res. 2016;8:2011-21 pubmed
  16. Chan W, Ismail H, Mayaki D, Sanchez V, Tiedemann K, Davis E, et al. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells. PLoS ONE. 2016;11:e0156994 pubmed publisher
  17. Lombardi R, Chen S, Ruggiero A, Gurha P, Czernuszewicz G, Willerson J, et al. Cardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene. Circ Res. 2016;119:41-54 pubmed publisher
  18. Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, et al. Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling. Acta Neuropathol. 2016;131:753-73 pubmed publisher
  19. Zhou Y, Williams J, Smallwood P, Nathans J. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina. PLoS ONE. 2015;10:e0143650 pubmed publisher
  20. Nasrallah R, Knezevic K, Thai T, Thomas S, Göttgens B, Lacaud G, et al. Endoglin potentiates nitric oxide synthesis to enhance definitive hematopoiesis. Biol Open. 2015;4:819-29 pubmed publisher
  21. Shi X, Zirbes K, Rasmussen T, Ferdous A, Garry M, Koyano Nakagawa N, et al. The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression. J Biol Chem. 2015;290:9614-25 pubmed publisher
  22. Forgèt M, Voorhees J, Cole S, Dakhlallah D, Patterson I, Gross A, et al. Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer. PLoS ONE. 2014;9:e98623 pubmed publisher
  23. Soblet J, Limaye N, Uebelhoer M, Boon L, Vikkula M. Variable Somatic TIE2 Mutations in Half of Sporadic Venous Malformations. Mol Syndromol. 2013;4:179-83 pubmed publisher