This is a Validated Antibody Database (VAD) review about mouse Stat5b, based on 29 published articles (read how Labome selects the articles), using Stat5b antibody in all methods. It is aimed to help Labome visitors find the most suited Stat5b antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Stat5b synonym: signal transducer and activator of transcription 5B; mammary gland factor STAT5B

Santa Cruz Biotechnology
mouse monoclonal (A-9)
  • ChIP-Seq; mouse; loading ...; fig 3c, 7b
Santa Cruz Biotechnology Stat5b antibody (Santa Cruz, sc-74442) was used in ChIP-Seq on mouse samples (fig 3c, 7b). Nucleic Acids Res (2018) ncbi
mouse monoclonal (G-2)
  • western blot; human; loading ...; fig 3a
Santa Cruz Biotechnology Stat5b antibody (Santa Cruz Biotechnology, sc-1656) was used in western blot on human samples (fig 3a). Oncoimmunology (2018) ncbi
mouse monoclonal (A-9)
  • immunoprecipitation; human; loading ...; fig 5b
Santa Cruz Biotechnology Stat5b antibody (Santa-Cruz, sc-74442) was used in immunoprecipitation on human samples (fig 5b). Nat Commun (2018) ncbi
mouse monoclonal (G-2)
  • western blot; mouse; 1:5000; loading ...; fig 1a
Santa Cruz Biotechnology Stat5b antibody (Santa Cruz, sc-1656) was used in western blot on mouse samples at 1:5000 (fig 1a). J Endocr Soc (2018) ncbi
mouse monoclonal (G-2)
  • western blot; mouse; fig 4
Santa Cruz Biotechnology Stat5b antibody (Santa Cruz, SC1656) was used in western blot on mouse samples (fig 4). Sci Rep (2016) ncbi
mouse monoclonal (G-2)
  • immunoprecipitation; human; loading ...; fig 2c
  • immunocytochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 1b
Santa Cruz Biotechnology Stat5b antibody (Santa Cruz, sc1656) was used in immunoprecipitation on human samples (fig 2c), in immunocytochemistry on human samples (fig 2a) and in western blot on human samples (fig 1b). Oncol Rep (2016) ncbi
mouse monoclonal (A-9)
  • chromatin immunoprecipitation; mouse; fig 7
Santa Cruz Biotechnology Stat5b antibody (Santa Cruz, sc-74442 X) was used in chromatin immunoprecipitation on mouse samples (fig 7). Nat Commun (2016) ncbi
mouse monoclonal (A-9)
  • western blot; human; fig 4
Santa Cruz Biotechnology Stat5b antibody (Santa Cruz Biotechnology, A-9) was used in western blot on human samples (fig 4). PLoS Genet (2016) ncbi
mouse monoclonal
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Santa Cruz Biotechnology Stat5b antibody (SCBT, G-2) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (G-2)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Santa Cruz Biotechnology Stat5b antibody (SCBT, G-2) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (G-2)
  • western blot; human; loading ...; fig 2d
Santa Cruz Biotechnology Stat5b antibody (Santa Cruz, 1656) was used in western blot on human samples (fig 2d). Sci Adv (2015) ncbi
mouse monoclonal (G-2)
  • chromatin immunoprecipitation; human
  • western blot; human
In order to test if deacetylase inhibitors target STAT5 or histone acetylation, Santa Cruz Biotechnology Stat5b antibody (Santa Cruz, sc-1656) was used in chromatin immunoprecipitation on human samples and in western blot on human samples . Nucleic Acids Res (2015) ncbi
mouse monoclonal (G-2)
  • western blot; mouse; 1:4000; fig 5
In order to elucidate the molecular mechanisms involved in the upregulation of ABCG2 during lactation, Santa Cruz Biotechnology Stat5b antibody (Santa Cruz Biotechnology, sc-1656) was used in western blot on mouse samples at 1:4000 (fig 5). Am J Physiol Endocrinol Metab (2014) ncbi
Invitrogen
mouse monoclonal (ST5b-10G1)
  • chromatin immunoprecipitation; human; loading ...; fig 4a
In order to explore the role of the JAK/STAT pathway in controlling Foxp3 in regulatory T cells, Invitrogen Stat5b antibody (Life Technologies, ST5b-10G1) was used in chromatin immunoprecipitation on human samples (fig 4a). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:100
In order to examine the daily rhythm of STAT3 protein, Invitrogen Stat5b antibody (Invitrogen, 71-2500) was used in immunohistochemistry - free floating section on rat samples at 1:100. J Neurosci Res (2016) ncbi
mouse monoclonal (ST5b-10G1)
  • EMSA; cow; fig 6
In order to investigate the role of CpG methylation in milk production, Invitrogen Stat5b antibody (Invitrogen, 13-5300) was used in EMSA on cow samples (fig 6). PLoS ONE (2014) ncbi
mouse monoclonal (ST5b-10G1)
  • ChIP-Seq; human; 12 ug
  • immunocytochemistry; human; 1:1000
  • western blot; human
In order to investigate genes regulated by STAT5A and/or STAT5B in human CD4(+) T cells, Invitrogen Stat5b antibody (Invitrogen, 135300) was used in ChIP-Seq on human samples at 12 ug, in immunocytochemistry on human samples at 1:1000 and in western blot on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • ChIP-Seq; human; fig 2
In order to examine how STAT3 and 5 regulate BCL6 transcription, Invitrogen Stat5b antibody (Zymed, 71-2500) was used in ChIP-Seq on human samples (fig 2). Mol Cell Biol (2013) ncbi
rabbit polyclonal
  • western blot; mouse; fig 6
In order to study KRAB-ZFP epigenetic mechanisms, Invitrogen Stat5b antibody (Invitrogen, 71-2500) was used in western blot on mouse samples (fig 6). Mol Cell Biol (2012) ncbi
mouse monoclonal (ST5b-10G1)
  • immunohistochemistry - free floating section; mouse; 1:3000; fig 3
In order to report that STAT5b and not STAT5a is the primary mediator prolactin signaling in the hypothalamus, Invitrogen Stat5b antibody (Zymed, 13-5300) was used in immunohistochemistry - free floating section on mouse samples at 1:3000 (fig 3). J Neuroendocrinol (2012) ncbi
mouse monoclonal (ST5b-10G1)
  • immunoprecipitation; human
  • western blot; human
In order to identify NCAM1 as a novel STAT5 target, Invitrogen Stat5b antibody (Invitrogen, 13-5300) was used in immunoprecipitation on human samples and in western blot on human samples . J Biol Chem (2006) ncbi
rabbit polyclonal
In order to assess the effects of STAT5 activation on BCL6 expression and function, Invitrogen Stat5b antibody (Zymed, 71-2500) was used . Oncogene (2007) ncbi
mouse monoclonal (ST5P-4A9)
  • western blot; rat; 1:500
In order to study the distribution of STAT proteins in the hypothalamus, Invitrogen Stat5b antibody (Zymed, 33-6000) was used in western blot on rat samples at 1:500. J Neuroendocrinol (2005) ncbi
rabbit polyclonal
In order to study the distribution of STAT proteins in the hypothalamus, Invitrogen Stat5b antibody (Zymed, 71-2500) was used . J Neuroendocrinol (2005) ncbi
rabbit polyclonal
In order to investigate STAT5-regulated genes, Invitrogen Stat5b antibody (Zymed, 71-2500) was used . J Biol Chem (2004) ncbi
mouse monoclonal (ST5b-10G1)
  • western blot; mouse
In order to investigate STAT5-regulated genes, Invitrogen Stat5b antibody (Zymed, 13-5300) was used in western blot on mouse samples . J Biol Chem (2004) ncbi
mouse monoclonal (ST5P-4A9)
  • western blot; human; fig 2
In order to identify pathways that are constitutively activated in a KIT-dependent manner in gastrointestinal stromal tumors, Invitrogen Stat5b antibody (Zymed, ST5P-4A9) was used in western blot on human samples (fig 2). Oncogene (2004) ncbi
mouse monoclonal (ST5-8F7)
  • western blot; human; fig 2
In order to identify pathways that are constitutively activated in a KIT-dependent manner in gastrointestinal stromal tumors, Invitrogen Stat5b antibody (Zymed, ST5-8F7) was used in western blot on human samples (fig 2). Oncogene (2004) ncbi
rabbit polyclonal
In order to examine the expression, tyrosine phosphorylation status and nuclear localization of Stat5a in human breast cancers, Invitrogen Stat5b antibody (Zymed, 71-2500) was used . Int J Cancer (2004) ncbi
mouse monoclonal (ST5b-10G1)
  • immunoprecipitation; human
  • western blot; human; 1:500
In order to examine the expression, tyrosine phosphorylation status and nuclear localization of Stat5a in human breast cancers, Invitrogen Stat5b antibody (Zymed, 13-5300) was used in immunoprecipitation on human samples and in western blot on human samples at 1:500. Int J Cancer (2004) ncbi
mouse monoclonal (ST5-8F7)
  • western blot; mouse; fig 4
In order to propose that PU.1 has an important role in regulating the proliferation of immature erythroid progenitors, Invitrogen Stat5b antibody (Zymed, 33-5900) was used in western blot on mouse samples (fig 4). Leuk Res (2004) ncbi
rabbit polyclonal
In order to demonstrate that STAT5 isoform expression, GM-CSF-induced STAT5 activation, and STAT5 target-gene expression are altered significantly during monocyte/macrophage differentiation, Invitrogen Stat5b antibody (Zymed, 71-2500) was used . J Leukoc Biol (2002) ncbi
EMD Millipore
rabbit monoclonal (A11W)
  • western blot; human; tbl s3
EMD Millipore Stat5b antibody (Millipore, A11W) was used in western blot on human samples (tbl s3). Cell Death Dis (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
EMD Millipore Stat5b antibody (Upstate/Millipore, 06-554) was used in chromatin immunoprecipitation on human samples . Oncogene (2015) ncbi
Articles Reviewed
  1. Lee H, Willi M, Shin H, Liu C, Hennighausen L. Progressing super-enhancer landscape during mammary differentiation controls tissue-specific gene regulation. Nucleic Acids Res. 2018;46:10796-10809 pubmed publisher
  2. Luo N, Formisano L, Gonzalez Ericsson P, Sanchez V, Dean P, Opalenik S, et al. Melanoma response to anti-PD-L1 immunotherapy requires JAK1 signaling, but not JAK2. Oncoimmunology. 2018;7:e1438106 pubmed publisher
  3. Sevin M, Kubovcakova L, Pernet N, Causse S, Vitte F, Villeval J, et al. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. Nat Commun. 2018;9:1431 pubmed publisher
  4. Jallow F, Brockman J, Helzer K, Rugowski D, Goffin V, Alarid E, et al. 17?-Estradiol and ICI182,780 Differentially Regulate STAT5 Isoforms in Female Mammary Epithelium, With Distinct Outcomes. J Endocr Soc. 2018;2:293-309 pubmed publisher
  5. Guo L, Costanzo Garvey D, Smith D, Zavorka M, Venable Kang M, MacDonald R, et al. Cell non-autonomous regulation of hepatic IGF-1 and neonatal growth by Kinase Suppressor of Ras 2 (KSR2). Sci Rep. 2016;6:32093 pubmed publisher
  6. Goldstein J, Burlion A, Zaragoza B, Sendeyo K, Polansky J, Huehn J, et al. Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression. PLoS ONE. 2016;11:e0153682 pubmed publisher
  7. Sumiyoshi H, Matsushita A, Nakamura Y, Matsuda Y, Ishiwata T, Naito Z, et al. Suppression of STAT5b in pancreatic cancer cells leads to attenuated gemcitabine chemoresistance, adhesion and invasion. Oncol Rep. 2016;35:3216-26 pubmed publisher
  8. Gomez Rodriguez J, Meylan F, Handon R, Hayes E, Anderson S, Kirby M, et al. Itk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4. Nat Commun. 2016;7:10857 pubmed publisher
  9. Kim J, He X, Orr B, Wutz G, Hill V, Peters J, et al. Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells Harboring Tumor-Derived Mutations in STAG2. PLoS Genet. 2016;12:e1005865 pubmed publisher
  10. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  11. Green A, Maciel T, Hospital M, Yin C, Mazed F, Townsend E, et al. Pim kinases modulate resistance to FLT3 tyrosine kinase inhibitors in FLT3-ITD acute myeloid leukemia. Sci Adv. 2015;1:e1500221 pubmed publisher
  12. Moravcová S, Červená K, Pačesová D, Bendová Z. Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide. J Neurosci Res. 2016;94:99-108 pubmed publisher
  13. Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
  14. Pinz S, Unser S, Buob D, Fischer P, Jobst B, Rascle A. Deacetylase inhibitors repress STAT5-mediated transcription by interfering with bromodomain and extra-terminal (BET) protein function. Nucleic Acids Res. 2015;43:3524-45 pubmed publisher
  15. Nguyen M, Boutinaud M, Pétridou B, Gabory A, Pannetier M, Chat S, et al. DNA methylation and transcription in a distal region upstream from the bovine AlphaS1 casein gene after once or twice daily milking. PLoS ONE. 2014;9:e111556 pubmed publisher
  16. Wu A, Yang M, Dalvi P, Turinsky A, Wang W, Butcher D, et al. Role of STAT5 and epigenetics in lactation-associated upregulation of multidrug transporter ABCG2 in the mammary gland. Am J Physiol Endocrinol Metab. 2014;307:E596-610 pubmed publisher
  17. Girardot M, Pecquet C, Chachoua I, Van Hees J, Guibert S, Ferrant A, et al. Persistent STAT5 activation in myeloid neoplasms recruits p53 into gene regulation. Oncogene. 2015;34:1323-32 pubmed publisher
  18. Kanai T, Seki S, Jenks J, Kohli A, Kawli T, Martin D, et al. Identification of STAT5A and STAT5B target genes in human T cells. PLoS ONE. 2014;9:e86790 pubmed publisher
  19. Walker S, Nelson E, Yeh J, Pinello L, Yuan G, Frank D. STAT5 outcompetes STAT3 to regulate the expression of the oncogenic transcriptional modulator BCL6. Mol Cell Biol. 2013;33:2879-90 pubmed publisher
  20. Krebs C, Schultz D, Robins D. The KRAB zinc finger protein RSL1 regulates sex- and tissue-specific promoter methylation and dynamic hormone-responsive chromatin configuration. Mol Cell Biol. 2012;32:3732-42 pubmed publisher
  21. Yip S, Eguchi R, Grattan D, Bunn S. Prolactin signalling in the mouse hypothalamus is primarily mediated by signal transducer and activator of transcription factor 5b but not 5a. J Neuroendocrinol. 2012;24:1484-91 pubmed publisher
  22. Nelson E, Walker S, Li W, Liu X, Frank D. Identification of human STAT5-dependent gene regulatory elements based on interspecies homology. J Biol Chem. 2006;281:26216-24 pubmed
  23. Walker S, Nelson E, Frank D. STAT5 represses BCL6 expression by binding to a regulatory region frequently mutated in lymphomas. Oncogene. 2007;26:224-33 pubmed
  24. Bennett E, McGuinness L, Gevers E, Thomas G, Robinson I, Davey H, et al. Hypothalamic STAT proteins: regulation of somatostatin neurones by growth hormone via STAT5b. J Neuroendocrinol. 2005;17:186-94 pubmed
  25. Nelson E, Walker S, Alvarez J, Frank D. Isolation of unique STAT5 targets by chromatin immunoprecipitation-based gene identification. J Biol Chem. 2004;279:54724-30 pubmed
  26. Duensing A, Medeiros F, McConarty B, Joseph N, Panigrahy D, Singer S, et al. Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs). Oncogene. 2004;23:3999-4006 pubmed
  27. Cotarla I, Ren S, Zhang Y, Gehan E, Singh B, Furth P. Stat5a is tyrosine phosphorylated and nuclear localized in a high proportion of human breast cancers. Int J Cancer. 2004;108:665-71 pubmed
  28. Fisher R, Slayton W, Chien C, Guthrie S, Bray C, Scott E. PU.1 supports proliferation of immature erythroid progenitors. Leuk Res. 2004;28:83-9 pubmed
  29. Lehtonen A, Matikainen S, Miettinen M, Julkunen I. Granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced STAT5 activation and target-gene expression during human monocyte/macrophage differentiation. J Leukoc Biol. 2002;71:511-9 pubmed