This is a Validated Antibody Database (VAD) review about mouse Spi1, based on 22 published articles (read how Labome selects the articles), using Spi1 antibody in all methods. It is aimed to help Labome visitors find the most suited Spi1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Spi1 synonym: Dis-1; Dis1; PU.1; Sfpi-1; Sfpi1; Spi-1; Tcfpu1; Tfpu.1

Santa Cruz Biotechnology
mouse monoclonal (C-3)
  • immunohistochemistry - free floating section; mouse; 1:200; loading ...; fig s2d
Santa Cruz Biotechnology Spi1 antibody (Santa Cruz, sc-390405) was used in immunohistochemistry - free floating section on mouse samples at 1:200 (fig s2d). Mol Neurodegener (2022) ncbi
mouse monoclonal (C-3)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4c
Santa Cruz Biotechnology Spi1 antibody (Santa, sc-390405) was used in immunohistochemistry - frozen section on mouse samples (fig 4c). Neuron (2021) ncbi
Abcam
domestic rabbit monoclonal (EPR22624-20)
  • western blot; human; 1:300; loading ...; fig 4a
Abcam Spi1 antibody (Abcam, ab227835) was used in western blot on human samples at 1:300 (fig 4a). Aging (Albany NY) (2021) ncbi
mouse monoclonal
  • immunocytochemistry; human; 1:200; fig s3c
Abcam Spi1 antibody (Abcam, ab88082) was used in immunocytochemistry on human samples at 1:200 (fig s3c). Mol Psychiatry (2017) ncbi
mouse monoclonal
  • western blot; mouse
Abcam Spi1 antibody (Abcam, ab88082) was used in western blot on mouse samples . Leukemia (2014) ncbi
Invitrogen
domestic rabbit monoclonal (E.388.3)
  • immunohistochemistry - frozen section; human; loading ...; fig 2a
In order to study the role of TH9 cells in ulcerative colitis, Invitrogen Spi1 antibody (Thermo Scientific, E.388.3) was used in immunohistochemistry - frozen section on human samples (fig 2a). Nat Immunol (2014) ncbi
Abnova
mouse monoclonal (2G1)
  • western blot; human; loading ...; fig 4
Abnova Spi1 antibody (Abnova, H00006688-M02) was used in western blot on human samples (fig 4). Cell Host Microbe (2017) ncbi
BioLegend
rat monoclonal (7C2C34)
  • flow cytometry; mouse; loading ...; fig 4c
BioLegend Spi1 antibody (Biolegend, 681308) was used in flow cytometry on mouse samples (fig 4c). elife (2018) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (9G7)
  • flow cytometry; mouse; fig s10h
Cell Signaling Technology Spi1 antibody (Cell Signaling, 2240S) was used in flow cytometry on mouse samples (fig s10h). Sci Adv (2022) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 3f
Cell Signaling Technology Spi1 antibody (Cell Signaling, 2266) was used in western blot on mouse samples at 1:1000 (fig 3f). J Immunother Cancer (2021) ncbi
domestic rabbit monoclonal (9G7)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 3b
Cell Signaling Technology Spi1 antibody (Cell Signaling, 9G7) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 3b). Anal Cell Pathol (Amst) (2020) ncbi
domestic rabbit polyclonal
  • flow cytometry; mouse; 1:1000; loading ...; fig s3b
  • immunohistochemistry; mouse; 1:500; loading ...; fig 1c
Cell Signaling Technology Spi1 antibody (CST, 2266) was used in flow cytometry on mouse samples at 1:1000 (fig s3b) and in immunohistochemistry on mouse samples at 1:500 (fig 1c). Nat Commun (2020) ncbi
domestic rabbit monoclonal (9G7)
  • ChIP-Seq; human; loading ...; fig 4c
  • chromatin immunoprecipitation; human; loading ...; fig 4c
Cell Signaling Technology Spi1 antibody (Cell Signaling, 2258) was used in ChIP-Seq on human samples (fig 4c) and in chromatin immunoprecipitation on human samples (fig 4c). Cell Rep (2019) ncbi
domestic rabbit monoclonal (9G7)
  • other; mouse; fig s1d
Cell Signaling Technology Spi1 antibody (Cell Signaling Technology, 2240) was used in other on mouse samples (fig s1d). Immunity (2019) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; mouse; loading ...; fig 4h
Cell Signaling Technology Spi1 antibody (Cell Signaling, 2258 s) was used in western blot on mouse samples (fig 4h). elife (2018) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; human; loading ...; fig 9h
Cell Signaling Technology Spi1 antibody (Cell Signaling, 2258) was used in western blot on human samples (fig 9h). J Clin Invest (2018) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; human; fig 5e
Cell Signaling Technology Spi1 antibody (Cell Signaling, 2258S) was used in western blot on human samples (fig 5e). Cell (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; fig 6
Cell Signaling Technology Spi1 antibody (Cell Signalling, 2266) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 6). Nat Commun (2016) ncbi
domestic rabbit monoclonal (9G7)
  • chromatin immunoprecipitation; human; loading ...; fig 2c
Cell Signaling Technology Spi1 antibody (Cell Signaling, 2258) was used in chromatin immunoprecipitation on human samples (fig 2c). J Hematol Oncol (2016) ncbi
domestic rabbit monoclonal (9G7)
  • immunocytochemistry; human; loading ...; fig s2a
Cell Signaling Technology Spi1 antibody (Cell Signaling Technology, 2258) was used in immunocytochemistry on human samples (fig s2a). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; mouse; 1:1000; fig 6
Cell Signaling Technology Spi1 antibody (Cell Signaling, 2258) was used in western blot on mouse samples at 1:1000 (fig 6). Nat Commun (2015) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; human
In order to study the molecular signaling responsible for retinoic acid-induced differentiation, Cell Signaling Technology Spi1 antibody (Cell Signaling, 2258) was used in western blot on human samples . PLoS ONE (2015) ncbi
domestic rabbit monoclonal (9G7)
  • immunohistochemistry - free floating section; mouse; 1:400; tbl 1
Cell Signaling Technology Spi1 antibody (Cell signalling, 2258S) was used in immunohistochemistry - free floating section on mouse samples at 1:400 (tbl 1). Brain Behav Immun (2015) ncbi
Articles Reviewed
  1. Welch G, Boix C, Schmauch E, Davila Velderrain J, Victor M, Dileep V, et al. Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration. Sci Adv. 2022;8:eabo4662 pubmed publisher
  2. Puntambekar S, Moutinho M, Lin P, Jadhav V, Tumbleson Brink D, Balaji A, et al. CX3CR1 deficiency aggravates amyloid driven neuronal pathology and cognitive decline in Alzheimer's disease. Mol Neurodegener. 2022;17:47 pubmed publisher
  3. Schanz O, Cornez I, Yajnanarayana S, David F, Peer S, Gruber T, et al. Tumor rejection in Cblb -/- mice depends on IL-9 and Th9 cells. J Immunother Cancer. 2021;9: pubmed publisher
  4. Xu Z, Cheng C, Kong R, Liu Y, Wang S, Ma Y, et al. S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn. Aging (Albany NY). 2021;13:15523-15537 pubmed publisher
  5. Safaiyan S, Besson Girard S, Kaya T, Cantuti Castelvetri L, Liu L, Ji H, et al. White matter aging drives microglial diversity. Neuron. 2021;109:1100-1117.e10 pubmed publisher
  6. Kovaleva O, Rashidova M, Samoilova D, Podlesnaya P, Mochalnikova V, Gratchev A. Immunosuppressive Phenotype of Esophagus Tumors Stroma. Anal Cell Pathol (Amst). 2020;2020:5424780 pubmed publisher
  7. Yu X, Liu H, Hamel K, Morvan M, Yu S, Leff J, et al. Dorsal root ganglion macrophages contribute to both the initiation and persistence of neuropathic pain. Nat Commun. 2020;11:264 pubmed publisher
  8. Senigl F, Maman Y, Dinesh R, Alinikula J, Seth R, Pecnova L, et al. Topologically Associated Domains Delineate Susceptibility to Somatic Hypermutation. Cell Rep. 2019;29:3902-3915.e8 pubmed publisher
  9. Chopin M, Lun A, Zhan Y, Schreuder J, Coughlan H, D Amico A, et al. Transcription Factor PU.1 Promotes Conventional Dendritic Cell Identity and Function via Induction of Transcriptional Regulator DC-SCRIPT. Immunity. 2019;50:77-90.e5 pubmed publisher
  10. Zhu H, Zhang L, Wu Y, Dong B, Guo W, Wang M, et al. T-ALL leukemia stem cell 'stemness' is epigenetically controlled by the master regulator SPI1. elife. 2018;7: pubmed publisher
  11. Chennupati V, Veiga D, Maslowski K, Andina N, Tardivel A, Yu E, et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest. 2018;128:1597-1614 pubmed publisher
  12. Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
  13. Hoffmann H, Schneider W, Blomen V, Scull M, Hovnanian A, Brummelkamp T, et al. Diverse Viruses Require the Calcium Transporter SPCA1 for Maturation and Spread. Cell Host Microbe. 2017;22:460-470.e5 pubmed publisher
  14. Sellgren C, Sheridan S, Gracias J, Xuan D, Fu T, Perlis R. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry. 2017;22:170-177 pubmed publisher
  15. Alivernini S, Kurowska Stolarska M, Tolusso B, Benvenuto R, Elmesmari A, Canestri S, et al. MicroRNA-155 influences B-cell function through PU.1 in rheumatoid arthritis. Nat Commun. 2016;7:12970 pubmed publisher
  16. Wei S, Zhao M, Wang X, Li Y, Wang K. PU.1 controls the expression of long noncoding RNA HOTAIRM1 during granulocytic differentiation. J Hematol Oncol. 2016;9:44 pubmed publisher
  17. Burda P, Vargova J, Curik N, Salek C, Papadopoulos G, Strouboulis J, et al. GATA-1 Inhibits PU.1 Gene via DNA and Histone H3K9 Methylation of Its Distal Enhancer in Erythroleukemia. PLoS ONE. 2016;11:e0152234 pubmed publisher
  18. Xiao X, Shi X, Fan Y, Zhang X, Wu M, Lan P, et al. GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation. Nat Commun. 2015;6:8266 pubmed publisher
  19. Bunaciu R, Jensen H, Macdonald R, Latocha D, Varner J, Yen A. 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells. PLoS ONE. 2015;10:e0135668 pubmed publisher
  20. Almolda B, de Labra C, Barrera I, Gruart A, Delgado Garcia J, Villacampa N, et al. Alterations in microglial phenotype and hippocampal neuronal function in transgenic mice with astrocyte-targeted production of interleukin-10. Brain Behav Immun. 2015;45:80-97 pubmed publisher
  21. Gerlach K, Hwang Y, Nikolaev A, Atreya R, Dornhoff H, Steiner S, et al. TH9 cells that express the transcription factor PU.1 drive T cell-mediated colitis via IL-9 receptor signaling in intestinal epithelial cells. Nat Immunol. 2014;15:676-86 pubmed publisher
  22. Zhou J, Wu J, Li B, Liu D, Yu J, Yan X, et al. PU.1 is essential for MLL leukemia partially via crosstalk with the MEIS/HOX pathway. Leukemia. 2014;28:1436-48 pubmed publisher