This is a Validated Antibody Database (VAD) review about mouse Sp1, based on 25 published articles (read how Labome selects the articles), using Sp1 antibody in all methods. It is aimed to help Labome visitors find the most suited Sp1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Sp1 synonym: 1110003E12Rik; AA450830; AI845540; Sp1-1

Knockout validation
EMD Millipore
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s1b
EMD Millipore Sp1 antibody (Millipore, 07-645) was used in western blot knockout validation on mouse samples (fig s1b). Epigenetics Chromatin (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-3)
  • ChIP-Seq; human; ; loading ...; fig 2a
Santa Cruz Biotechnology Sp1 antibody (Santa Cruz, sc-17824) was used in ChIP-Seq on human samples at (fig 2a). elife (2019) ncbi
mouse monoclonal (1C6)
  • western blot; human; loading ...; fig s2
Santa Cruz Biotechnology Sp1 antibody (SantaCruz, sc-420) was used in western blot on human samples (fig s2). Biomed Pharmacother (2018) ncbi
mouse monoclonal (1C6)
  • western blot; mouse; loading ...; fig 6b
Santa Cruz Biotechnology Sp1 antibody (Santa Cruz Biotechnology, sc-420) was used in western blot on mouse samples (fig 6b). Stem Cells Int (2017) ncbi
mouse monoclonal (E-3)
  • ChIP-Seq; human; tbl 1
Santa Cruz Biotechnology Sp1 antibody (SantaCruz, SC-17824) was used in ChIP-Seq on human samples (tbl 1). Biochim Biophys Acta Gene Regul Mech (2017) ncbi
mouse monoclonal (E-3)
  • chromatin immunoprecipitation; human; fig 1
Santa Cruz Biotechnology Sp1 antibody (Santa Cruz, sc-17824) was used in chromatin immunoprecipitation on human samples (fig 1). Oncol Lett (2016) ncbi
mouse monoclonal (1C6)
  • western blot; mouse; 1:100; fig 4
Santa Cruz Biotechnology Sp1 antibody (santa Cruz, sc-420) was used in western blot on mouse samples at 1:100 (fig 4). Front Aging Neurosci (2015) ncbi
mouse monoclonal (1C6)
  • western blot; human
Santa Cruz Biotechnology Sp1 antibody (Santa Cruz, sc-420) was used in western blot on human samples . Nat Commun (2015) ncbi
mouse monoclonal (1C6)
  • western blot; human; fig 2
Santa Cruz Biotechnology Sp1 antibody (Santa Cruz Biotechnology, sc-420) was used in western blot on human samples (fig 2). Nucleic Acids Res (2015) ncbi
mouse monoclonal (E-3)
  • EMSA; rat
  • EMSA; mouse
In order to study the upregulation of pancreatic beta cell ARAP1 by a functional regulatory DNA variant at a type 2 diabetes locus, Santa Cruz Biotechnology Sp1 antibody (Santa Cruz Biotechnology, sc-17824X) was used in EMSA on rat samples and in EMSA on mouse samples . Am J Hum Genet (2014) ncbi
mouse monoclonal (E-3)
Santa Cruz Biotechnology Sp1 antibody (Santa Cruz, sc-17824) was used . Oncogene (2014) ncbi
mouse monoclonal (1C6)
  • western blot; human
Santa Cruz Biotechnology Sp1 antibody (Santa Cruz Biotechnology, sc-420) was used in western blot on human samples . Mol Oncol (2013) ncbi
Abcam
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 5d
Abcam Sp1 antibody (Abcam, ab13370) was used in chromatin immunoprecipitation on human samples (fig 5d). Biol Open (2019) ncbi
domestic rabbit polyclonal
  • RNA immunoprecipitation; human; fig 6b
Abcam Sp1 antibody (Abcam, ab13370) was used in RNA immunoprecipitation on human samples (fig 6b). Nucleic Acids Res (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 2a
In order to study the efffect of H2S and homocysteine on cystathionine beta synthase and cystathionine gamma lyase, Abcam Sp1 antibody (Abcam, ab59257) was used in western blot on mouse samples (fig 2a). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3c
Abcam Sp1 antibody (Abcam, ab13370) was used in western blot on human samples (fig 3c). Oncogene (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (48G2)
  • immunocytochemistry; human; 1:200; loading ...; fig 4c
Cell Signaling Technology Sp1 antibody (Cell Signaling, 3726S) was used in immunocytochemistry on human samples at 1:200 (fig 4c). Cell Mol Gastroenterol Hepatol (2019) ncbi
domestic rabbit monoclonal (48G2)
  • western blot; human; 1:1000; loading ...; fig 4e
Cell Signaling Technology Sp1 antibody (Cell Signaling Technology, 3726) was used in western blot on human samples at 1:1000 (fig 4e). Nat Commun (2017) ncbi
domestic rabbit monoclonal (48G2)
  • western blot; rat; loading ...; fig 5a
Cell Signaling Technology Sp1 antibody (cst, 3726) was used in western blot on rat samples (fig 5a). J Cell Biol (2015) ncbi
EMD Millipore
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s1b
EMD Millipore Sp1 antibody (Millipore, 07-645) was used in western blot knockout validation on mouse samples (fig s1b). Epigenetics Chromatin (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; fig s6d
  • western blot; mouse; loading ...; fig s5e
EMD Millipore Sp1 antibody (EMD Millipore, 07-645) was used in immunocytochemistry on mouse samples (fig s6d) and in western blot on mouse samples (fig s5e). Cell (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 2a
In order to study the efffect of H2S and homocysteine on cystathionine beta synthase and cystathionine gamma lyase, EMD Millipore Sp1 antibody (Millipore, 07-645) was used in western blot on mouse samples (fig 2a). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • EMSA; human; loading ...; fig 5d
EMD Millipore Sp1 antibody (Upstate-Millipore, 07-645) was used in EMSA on human samples (fig 5d). Epigenetics (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 4d
In order to research the role of vacuolar-type H+-ATPase complex in liver in the accumulation of autophagic vacuoles and lysosomes, EMD Millipore Sp1 antibody (Millipore, ABE135) was used in western blot on mouse samples (fig 4d). Autophagy (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 5f
  • EMSA; mouse; loading ...; fig 5e
  • western blot; mouse; 1:1000; loading ...; fig 5b
In order to find that CD4+ T cells from DOCK8-deficient mice produce large amounts of IL-31, EMD Millipore Sp1 antibody (Millipore, 07-645) was used in chromatin immunoprecipitation on mouse samples (fig 5f), in EMSA on mouse samples (fig 5e) and in western blot on mouse samples at 1:1000 (fig 5b). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • EMSA; mouse; loading ...; fig 7c
EMD Millipore Sp1 antibody (Upstate, 07-645) was used in EMSA on mouse samples (fig 7c). Reprod Biol Endocrinol (2016) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; fig s5e
In order to investigate the co-regulation of Brg1 and Smarcal1 and their transcriptional regulation of Atp-dependent chromatin remodeling factors, EMD Millipore Sp1 antibody (Millipore, 07-645) was used in chromatin immunoprecipitation on human samples (fig s5e). Sci Rep (2016) ncbi
Articles Reviewed
  1. Dremel S, DeLuca N. Herpes simplex viral nucleoprotein creates a competitive transcriptional environment facilitating robust viral transcription and host shut off. elife. 2019;8: pubmed publisher
  2. Gilmour J, O Connor L, Middleton C, Keane P, Gillemans N, Cazier J, et al. Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors. Epigenetics Chromatin. 2019;12:33 pubmed publisher
  3. Alim I, Caulfield J, Chen Y, Swarup V, Geschwind D, Ivanova E, et al. Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke. Cell. 2019;177:1262-1279.e25 pubmed publisher
  4. Chang Graham A, Danhof H, Engevik M, Tomaro Duchesneau C, Karandikar U, Estes M, et al. Human Intestinal Enteroids With Inducible Neurogenin-3 Expression as a Novel Model of Gut Hormone Secretion. Cell Mol Gastroenterol Hepatol. 2019;: pubmed publisher
  5. Hassan A, Bagu E, Lévesque M, Patten S, Benhadjeba S, Edjekouane L, et al. The 17β-estradiol induced upregulation of the adhesion G-protein coupled receptor (ADGRG7) is modulated by ESRα and SP1 complex. Biol Open. 2019;8: pubmed publisher
  6. Yu Y, Shang R, Chen Y, Li J, Liang Z, Hu J, et al. Tumor suppressive ZBTB4 inhibits cell growth by regulating cell cycle progression and apoptosis in Ewing sarcoma. Biomed Pharmacother. 2018;100:108-115 pubmed publisher
  7. Zhang X, Huang G, Xie Y, He J, Guo J, Xu X, et al. The interaction of lncRNA EZR-AS1 with SMYD3 maintains overexpression of EZR in ESCC cells. Nucleic Acids Res. 2018;46:1793-1809 pubmed publisher
  8. Nandi S, Mishra P. H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes. Sci Rep. 2017;7:3639 pubmed publisher
  9. Nardone G, Oliver De La Cruz J, Vrbsky J, Martini C, Pribyl J, Skladal P, et al. YAP regulates cell mechanics by controlling focal adhesion assembly. Nat Commun. 2017;8:15321 pubmed publisher
  10. Po A, Begalli F, Abballe L, Alfano V, Besharat Z, Catanzaro G, et al. ?-Arrestin1/miR-326 Transcription Unit Is Epigenetically Regulated in Neural Stem Cells Where It Controls Stemness and Growth Arrest. Stem Cells Int. 2017;2017:5274171 pubmed publisher
  11. Hu T, Zhu X, Pi W, Yu M, Shi H, Tuan D. Hypermethylated LTR retrotransposon exhibits enhancer activity. Epigenetics. 2017;12:226-237 pubmed publisher
  12. Kissing S, Rudnik S, Damme M, Lüllmann Rauch R, Ichihara A, Kornak U, et al. Disruption of the vacuolar-type H+-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes. Autophagy. 2017;13:670-685 pubmed publisher
  13. Yamamura K, Uruno T, Shiraishi A, Tanaka Y, Ushijima M, Nakahara T, et al. The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. Nat Commun. 2017;8:13946 pubmed publisher
  14. Suske G. NF-Y and SP transcription factors - New insights in a long-standing liaison. Biochim Biophys Acta Gene Regul Mech. 2017;1860:590-597 pubmed publisher
  15. Deng X, Shao G, Zhang H, Li C, Zhang D, Cheng L, et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene. 2017;36:1223-1231 pubmed publisher
  16. McDonald E, Smith J, Cederberg R, White B. Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-B, specificity protein (SP)1-like and GATA-4 binding sites. Reprod Biol Endocrinol. 2016;14:36 pubmed publisher
  17. Zheng C, Yang K, Zhang M, Zou M, Bai E, Ma Q, et al. Specific protein 1 depletion attenuates glucose uptake and proliferation of human glioma cells by regulating GLUT3 expression. Oncol Lett. 2016;12:125-131 pubmed
  18. Haokip D, Goel I, Arya V, Sharma T, Kumari R, Priya R, et al. Transcriptional Regulation of Atp-Dependent Chromatin Remodeling Factors: Smarcal1 and Brg1 Mutually Co-Regulate Each Other. Sci Rep. 2016;6:20532 pubmed publisher
  19. Hou X, Adeosun S, Zhang Q, Barlow B, Brents M, Zheng B, et al. Differential contributions of ApoE4 and female sex to BACE1 activity and expression mediate Aβ deposition and learning and memory in mouse models of Alzheimer's disease. Front Aging Neurosci. 2015;7:207 pubmed publisher
  20. Tan K, Fujimoto M, Takii R, Takaki E, Hayashida N, Nakai A. Mitochondrial SSBP1 protects cells from proteotoxic stresses by potentiating stress-induced HSF1 transcriptional activity. Nat Commun. 2015;6:6580 pubmed publisher
  21. Wu Z, Wang C, Bai M, Li X, Mei Q, Li X, et al. An LRP16-containing preassembly complex contributes to NF-κB activation induced by DNA double-strand breaks. Nucleic Acids Res. 2015;43:3167-79 pubmed publisher
  22. Colombelli C, Palmisano M, Eshed Eisenbach Y, Zambroni D, Pavoni E, Ferri C, et al. Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin. J Cell Biol. 2015;208:313-29 pubmed publisher
  23. Kulzer J, Stitzel M, Morken M, Huyghe J, Fuchsberger C, Kuusisto J, et al. A common functional regulatory variant at a type 2 diabetes locus upregulates ARAP1 expression in the pancreatic beta cell. Am J Hum Genet. 2014;94:186-97 pubmed publisher
  24. Zhu Y, Yao Z, Wu Z, Mei Y, Wu M. Role of tumor necrosis factor alpha-induced protein 1 in paclitaxel resistance. Oncogene. 2014;33:3246-55 pubmed publisher
  25. Li L, Yang G, Ren C, Tanimoto R, Hirayama T, Wang J, et al. Glioma pathogenesis-related protein 1 induces prostate cancer cell death through Hsc70-mediated suppression of AURKA and TPX2. Mol Oncol. 2013;7:484-96 pubmed publisher