This is a Validated Antibody Database (VAD) review about mouse Sirt5, based on 4 published articles (read how Labome selects the articles), using Sirt5 antibody in all methods. It is aimed to help Labome visitors find the most suited Sirt5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Sirt5 synonym: 0610012J09Rik; 1500032M05Rik; AV001953; NAD-dependent protein deacylase sirtuin-5, mitochondrial; NAD-dependent deacetylase sirtuin-5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial

Knockout validation
Cell Signaling Technology
rabbit monoclonal (D8C3)
  • western blot; human; fig 3
  • western blot knockout validation; mouse; fig 3
Cell Signaling Technology Sirt5 antibody (Cell Signaling, 8782) was used in western blot on human samples (fig 3) and in western blot knockout validation on mouse samples (fig 3). Proc Natl Acad Sci U S A (2016) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; fig 1
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 1
Abcam Sirt5 antibody (abcam, 13697) was used in western blot on mouse samples (fig 1), in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 1). Autophagy (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Abcam Sirt5 antibody (Abcam, ab13697) was used in western blot on human samples at 1:1000. Biochim Biophys Acta (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D8C3)
  • western blot; mouse; 1:500; loading ...; fig 1e
Cell Signaling Technology Sirt5 antibody (Cell Signaling Technology, 8782) was used in western blot on mouse samples at 1:500 (fig 1e). Sci Rep (2019) ncbi
rabbit monoclonal (D8C3)
  • western blot; human; fig 3
  • western blot knockout validation; mouse; fig 3
Cell Signaling Technology Sirt5 antibody (Cell Signaling, 8782) was used in western blot on human samples (fig 3) and in western blot knockout validation on mouse samples (fig 3). Proc Natl Acad Sci U S A (2016) ncbi
Articles Reviewed
  1. Lin J, Lin J, Chen H, Chen T, Apte R. Combined SIRT3 and SIRT5 deletion is associated with inner retinal dysfunction in a mouse model of type 1 diabetes. Sci Rep. 2019;9:3799 pubmed publisher
  2. Sadhukhan S, Liu X, Ryu D, Nelson O, Stupinski J, Li Z, et al. Metabolomics-assisted proteomics identifies succinylation and SIRT5 as important regulators of cardiac function. Proc Natl Acad Sci U S A. 2016;113:4320-5 pubmed publisher
  3. Polletta L, Vernucci E, Carnevale I, Arcangeli T, Rotili D, Palmerio S, et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy. 2015;11:253-70 pubmed publisher
  4. Kim T, Kim H, Kang Y, Yoon S, Lee J, Choi W, et al. Psammaplin A induces Sirtuin 1-dependent autophagic cell death in doxorubicin-resistant MCF-7/adr human breast cancer cells and xenografts. Biochim Biophys Acta. 2015;1850:401-10 pubmed publisher