This is a Validated Antibody Database (VAD) review about mouse Sirpa, based on 22 published articles (read how Labome selects the articles), using Sirpa antibody in all methods. It is aimed to help Labome visitors find the most suited Sirpa antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Sirpa synonym: AI835480; Bit; CD172a; P84; Ptpns1; SHP-1; SHPS-1; SIRP; tyrosine-protein phosphatase non-receptor type substrate 1; CD172 antigen-like family member A; SHP substrate 1; brain Ig-like molecule with tyrosine-based activation motifs; brain immunological-like with tyrosine-based motifs; inhibitory receptor SHPS-1; mSIRP-alpha1; myD-1 antigen; protein tyrosine phosphatase, non-receptor type substrate 1; signal-regulatory protein alpha-1; sirp-alpha-1

Knockout validation
BD Biosciences
rat monoclonal (P84)
  • western blot knockout validation; mouse; fig 1a
BD Biosciences Sirpa antibody (BD Biosciences, P84) was used in western blot knockout validation on mouse samples (fig 1a). Proc Natl Acad Sci U S A (2016) ncbi
Invitrogen
rat monoclonal (P84)
  • flow cytometry; mouse; loading ...; fig s2b
Invitrogen Sirpa antibody (eBioscience, 46-1721-82) was used in flow cytometry on mouse samples (fig s2b). Cell (2019) ncbi
rat monoclonal (P84)
  • flow cytometry; mouse; 1 ug/ml; loading ...; fig 4f
Invitrogen Sirpa antibody (ThermoFish er Scientific, 46-1721-82) was used in flow cytometry on mouse samples at 1 ug/ml (fig 4f). Science (2019) ncbi
rat monoclonal (P84)
  • flow cytometry; mouse; fig 1c
Invitrogen Sirpa antibody (eBiosciences, P84) was used in flow cytometry on mouse samples (fig 1c). Eur J Immunol (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; fig 3b
  • western blot; human; fig 4d
In order to identify genes that contribute to human papilloma virus-16 associated oropharyngeal cancer, Invitrogen Sirpa antibody (Thermo Scientific, PA5-29544) was used in immunohistochemistry - paraffin section on human samples (fig 3b) and in western blot on human samples (fig 4d). Sci Rep (2017) ncbi
rat monoclonal (P84)
  • flow cytometry; mouse; loading ...; fig 2a
In order to determine the role of RelB in classical dendritic cell and myeloid development, Invitrogen Sirpa antibody (eBioscience, P84) was used in flow cytometry on mouse samples (fig 2a). Proc Natl Acad Sci U S A (2017) ncbi
rat monoclonal (P84)
  • western blot; human
  • flow cytometry; mouse; loading ...; fig 4a
  • western blot; mouse
In order to discover that SIRPalpha is highly expressed in human renal cell carcinoma and melanoma, Invitrogen Sirpa antibody (eBioscience, P84) was used in western blot on human samples , in flow cytometry on mouse samples (fig 4a) and in western blot on mouse samples . JCI Insight (2017) ncbi
rat monoclonal (P84)
  • flow cytometry; mouse; fig 2
In order to determine the contribution of different SP-R210 isoforms to various macrophage functions, Invitrogen Sirpa antibody (eBioscience, P84) was used in flow cytometry on mouse samples (fig 2). PLoS ONE (2015) ncbi
rat monoclonal (P84)
  • flow cytometry; mouse; fig 4a
In order to generate and characterize Hoxb8-FL cells, Invitrogen Sirpa antibody (eBiosciences, P84) was used in flow cytometry on mouse samples (fig 4a). Nat Methods (2013) ncbi
BioLegend
rat monoclonal (P84)
  • flow cytometry; mouse; loading ...; fig s2a
BioLegend Sirpa antibody (Biolegend, P84) was used in flow cytometry on mouse samples (fig s2a). Science (2018) ncbi
mouse monoclonal (OX-41)
  • flow cytometry; rat; 1:400; loading ...; fig 1b
BioLegend Sirpa antibody (BioLegend, OX-41) was used in flow cytometry on rat samples at 1:400 (fig 1b). J Immunol (2018) ncbi
mouse monoclonal (OX-41)
  • flow cytometry; rat; loading ...; fig 1a
BioLegend Sirpa antibody (Biolegend, 204706) was used in flow cytometry on rat samples (fig 1a). Stem Cell Reports (2018) ncbi
rat monoclonal (P84)
  • flow cytometry; mouse; loading ...; fig s1b
BioLegend Sirpa antibody (Biolegend, P84) was used in flow cytometry on mouse samples (fig s1b). Proc Natl Acad Sci U S A (2017) ncbi
rat monoclonal (P84)
  • immunocytochemistry; human; loading ...; fig s1
In order to discover that SIRPalpha is highly expressed in human renal cell carcinoma and melanoma, BioLegend Sirpa antibody (BioLegend, P84) was used in immunocytochemistry on human samples (fig s1). JCI Insight (2017) ncbi
mouse monoclonal (OX-41)
  • flow cytometry; rat; fig 1
In order to characterize rat myeloid-derived suppressor cells in a mammary carcinoma model, BioLegend Sirpa antibody (Biolegend, OX-41) was used in flow cytometry on rat samples (fig 1). Cell Immunol (2015) ncbi
Bio-Rad
mouse monoclonal (4C7)
  • immunocytochemistry; human; loading ...; fig 2a
In order to determine the localization of FcgammaRI, FcgammaRII, and SIRPalpha in macrophages, Bio-Rad Sirpa antibody (AbD Serotec, 4C7) was used in immunocytochemistry on human samples (fig 2a). J Cell Biol (2017) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry - frozen section; human; loading ...; fig 1b
In order to discover that SIRPalpha is highly expressed in human renal cell carcinoma and melanoma, Abcam Sirpa antibody (Abcam, ab53721) was used in immunohistochemistry - frozen section on human samples (fig 1b). JCI Insight (2017) ncbi
BD Biosciences
rat monoclonal (P84)
  • flow cytometry; mouse; loading ...; fig e3a
In order to investigate the function of SLAMF in phagocytosis of haematopoietic tumour cells, BD Biosciences Sirpa antibody (BD Biosciences, P84) was used in flow cytometry on mouse samples (fig e3a). Nature (2017) ncbi
rat monoclonal (P84)
  • flow cytometry; mouse; loading ...; fig 1b
In order to find a role for RAB43 in cross-presentation by classical dendritic cells, BD Biosciences Sirpa antibody (BD, P84) was used in flow cytometry on mouse samples (fig 1b). J Exp Med (2016) ncbi
rat monoclonal (P84)
  • western blot knockout validation; mouse; fig 1a
BD Biosciences Sirpa antibody (BD Biosciences, P84) was used in western blot knockout validation on mouse samples (fig 1a). Proc Natl Acad Sci U S A (2016) ncbi
rat monoclonal (P84)
  • flow cytometry; mouse; loading ...; fig 8a
In order to explore the relationships among beta8 integrin, TGF-beta, and intestinal dendritic cells, BD Biosciences Sirpa antibody (BD Biosciences, P84) was used in flow cytometry on mouse samples (fig 8a). J Immunol (2016) ncbi
rat monoclonal (P84)
  • flow cytometry; mouse; fig 1
In order to investigate the role of FAT10 in thymic antigen presentation, BD Biosciences Sirpa antibody (BD Biosciences., P84) was used in flow cytometry on mouse samples (fig 1). J Immunol (2015) ncbi
EMD Millipore
mouse monoclonal (OX-41)
  • immunohistochemistry - frozen section; mouse; 1:200; fig 2
EMD Millipore Sirpa antibody (Millipore, MAB1407P) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 2). Sci Rep (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
EMD Millipore Sirpa antibody (Millipore, 06-729) was used in chromatin immunoprecipitation on human samples . PLoS ONE (2014) ncbi
ProSci
rabbit polyclonal
  • western blot; mouse; loading ...; fig 2b
ProSci Sirpa antibody (ProSci, 1125) was used in western blot on mouse samples (fig 2b). Sci Rep (2017) ncbi
Articles Reviewed
  1. Liu Z, Gu Y, Chakarov S, Blériot C, Kwok I, Chen X, et al. Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells. Cell. 2019;178:1509-1525.e19 pubmed publisher
  2. Persson E, Verstraete K, Heyndrickx I, Gevaert E, Aegerter H, Percier J, et al. Protein crystallization promotes type 2 immunity and is reversible by antibody treatment. Science. 2019;364: pubmed publisher
  3. Choi H, Suwanpradid J, Kim I, Staats H, Haniffa M, Macleod A, et al. Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles. Science. 2018;362: pubmed publisher
  4. Pridans C, Raper A, Davis G, Alves J, Sauter K, Lefèvre L, et al. Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus. J Immunol. 2018;201:2683-2699 pubmed publisher
  5. Yang X, Zhou J, He J, Liu J, Wang H, Liu Y, et al. An Immune System-Modified Rat Model for Human Stem Cell Transplantation Research. Stem Cell Reports. 2018;11:514-521 pubmed publisher
  6. Herndler Brandstetter D, Shan L, Yao Y, Stecher C, Plajer V, Lietzenmayer M, et al. Humanized mouse model supports development, function, and tissue residency of human natural killer cells. Proc Natl Acad Sci U S A. 2017;114:E9626-E9634 pubmed publisher
  7. Anderson D, Grajales Reyes G, Satpathy A, Vasquez Hueichucura C, Murphy T, Murphy K. Revisiting the specificity of the MHC class?II transactivator CIITA in classical murine dendritic cells in vivo. Eur J Immunol. 2017;47:1317-1323 pubmed publisher
  8. Chen J, Zhong M, Guo H, Davidson D, Mishel S, Lu Y, et al. SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin. Nature. 2017;544:493-497 pubmed publisher
  9. Shirakabe K, Omura T, Shibagaki Y, Mihara E, Homma K, Kato Y, et al. Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation. Sci Rep. 2017;7:46174 pubmed publisher
  10. Kannan A, Hertweck K, Philley J, Wells R, Dasgupta S. Genetic Mutation and Exosome Signature of Human Papilloma Virus Associated Oropharyngeal Cancer. Sci Rep. 2017;7:46102 pubmed publisher
  11. Briseño C, Gargaro M, Durai V, Davidson J, Theisen D, Anderson D, et al. Deficiency of transcription factor RelB perturbs myeloid and DC development by hematopoietic-extrinsic mechanisms. Proc Natl Acad Sci U S A. 2017;114:3957-3962 pubmed publisher
  12. Lopes F, Bálint Å, Valvo S, Felce J, Hessel E, Dustin M, et al. Membrane nanoclusters of FcγRI segregate from inhibitory SIRPα upon activation of human macrophages. J Cell Biol. 2017;216:1123-1141 pubmed publisher
  13. Yanagita T, Murata Y, Tanaka D, Motegi S, Arai E, Daniwijaya E, et al. Anti-SIRPα antibodies as a potential new tool for cancer immunotherapy. JCI Insight. 2017;2:e89140 pubmed publisher
  14. Kretzer N, Theisen D, Tussiwand R, Briseño C, Grajales Reyes G, Wu X, et al. RAB43 facilitates cross-presentation of cell-associated antigens by CD8?+ dendritic cells. J Exp Med. 2016;213:2871-2883 pubmed
  15. Bian Z, Shi L, Guo Y, Lv Z, Tang C, Niu S, et al. Cd47-Sirp? interaction and IL-10 constrain inflammation-induced macrophage phagocytosis of healthy self-cells. Proc Natl Acad Sci U S A. 2016;113:E5434-43 pubmed publisher
  16. Boucard Jourdin M, Kugler D, Endale Ahanda M, This S, De Calisto J, Zhang A, et al. ?8 Integrin Expression and Activation of TGF-? by Intestinal Dendritic Cells Are Determined by Both Tissue Microenvironment and Cell Lineage. J Immunol. 2016;197:1968-78 pubmed publisher
  17. Buerger S, Herrmann V, Mundt S, Trautwein N, Groettrup M, Basler M. The Ubiquitin-like Modifier FAT10 Is Selectively Expressed in Medullary Thymic Epithelial Cells and Modifies T Cell Selection. J Immunol. 2015;195:4106-16 pubmed publisher
  18. Yang L, Carrillo M, Wu Y, DiAngelo S, Silveyra P, Umstead T, et al. SP-R210 (Myo18A) Isoforms as Intrinsic Modulators of Macrophage Priming and Activation. PLoS ONE. 2015;10:e0126576 pubmed publisher
  19. Dölen Y, Gunaydin G, Esendagli G, Guc D. Granulocytic subset of myeloid derived suppressor cells in rats with mammary carcinoma. Cell Immunol. 2015;295:29-35 pubmed publisher
  20. Nagai J, Kitamura Y, Owada K, Yamashita N, Takei K, Goshima Y, et al. Crmp4 deletion promotes recovery from spinal cord injury by neuroprotection and limited scar formation. Sci Rep. 2015;5:8269 pubmed publisher
  21. Huichalaf C, Micheloni S, Ferri G, Caccia R, Gabellini D. DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level. PLoS ONE. 2014;9:e115278 pubmed publisher
  22. Redecke V, Wu R, Zhou J, Finkelstein D, Chaturvedi V, High A, et al. Hematopoietic progenitor cell lines with myeloid and lymphoid potential. Nat Methods. 2013;10:795-803 pubmed publisher