This is a Validated Antibody Database (VAD) review about mouse Shh, based on 22 published articles (read how Labome selects the articles), using Shh antibody in all methods. It is aimed to help Labome visitors find the most suited Shh antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Shh synonym: 9530036O11Rik; Dsh; Hhg1; Hx; Hxl3; M100081; ShhNC

Knockout validation
Developmental Studies Hybridoma Bank
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; mouse; 1:20; loading ...; fig s8b
  • immunohistochemistry - paraffin section; mouse; 1:20; loading ...; fig 6b
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on mouse samples at 1:20 (fig s8b) and in immunohistochemistry - paraffin section on mouse samples at 1:20 (fig 6b). PLoS Genet (2017) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; Common quail; loading ...; fig 6c
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on Common quail samples (fig 6c). Front Cell Dev Biol (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-1)
  • western blot; human; 1:500; loading ...; fig s5a
Santa Cruz Biotechnology Shh antibody (Santa Cruz, sc-365112) was used in western blot on human samples at 1:500 (fig s5a). Front Immunol (2022) ncbi
Invitrogen
mouse monoclonal (5H4)
  • western blot; mouse; 1:1000; loading ...; fig 6m
Invitrogen Shh antibody (Thermo Fisher, 5H4) was used in western blot on mouse samples at 1:1000 (fig 6m). Adv Sci (Weinh) (2021) ncbi
Novus Biologicals
mouse monoclonal (5H4)
  • immunocytochemistry; human; 1:500; fig 1c
Novus Biologicals Shh antibody (Novus, NBP2-22126) was used in immunocytochemistry on human samples at 1:500 (fig 1c). Int J Mol Sci (2020) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; rat; 1:1000; loading ...; fig 5a
Abcam Shh antibody (Abcam, ab19897) was used in western blot on rat samples at 1:1000 (fig 5a). Neurochem Res (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C9C5)
  • western blot; human; 1:1000; loading ...; fig s5c
Cell Signaling Technology Shh antibody (Cell Signaling, 2207) was used in western blot on human samples at 1:1000 (fig s5c). Mol Cancer Res (2021) ncbi
domestic rabbit monoclonal (C9C5)
  • western blot; black ferret; 1:2500; loading ...; fig 6g, 6h
  • western blot; mouse; 1:2500; loading ...; fig 6g, 6h
Cell Signaling Technology Shh antibody (Cell Signaling, 2207) was used in western blot on black ferret samples at 1:2500 (fig 6g, 6h) and in western blot on mouse samples at 1:2500 (fig 6g, 6h). elife (2020) ncbi
domestic rabbit monoclonal (C9C5)
  • western blot; human; loading ...; fig 5
Cell Signaling Technology Shh antibody (Cell Signaling, 2207) was used in western blot on human samples (fig 5). Mol Clin Oncol (2016) ncbi
domestic rabbit monoclonal (C9C5)
  • western blot; mouse; fig 4
Cell Signaling Technology Shh antibody (Cell Signaling, 2207) was used in western blot on mouse samples (fig 4). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse
In order to investigate the role of LZTFL1 in lung oncogenesis, Cell Signaling Technology Shh antibody (Cell signaling, 2287) was used in western blot on mouse samples . Oncogene (2016) ncbi
domestic rabbit monoclonal (C9C5)
  • western blot; chicken; 1:600
Cell Signaling Technology Shh antibody (Cell Signaling, C9C5) was used in western blot on chicken samples at 1:600. Int J Mol Sci (2015) ncbi
domestic rabbit monoclonal (C9C5)
  • immunohistochemistry - paraffin section; mouse; 1:100
  • western blot; mouse; 1:500
Cell Signaling Technology Shh antibody (Cell Signaling Technology, 2207) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 and in western blot on mouse samples at 1:500. Mol Neurobiol (2016) ncbi
domestic rabbit monoclonal (C9C5)
  • western blot; mouse; 1:1000
Cell Signaling Technology Shh antibody (Cell Signaling, 2207) was used in western blot on mouse samples at 1:1000. Toxicol Appl Pharmacol (2014) ncbi
domestic rabbit monoclonal (C9C5)
  • western blot; human
Cell Signaling Technology Shh antibody (Cell Signaling Technology, 2207) was used in western blot on human samples . PLoS ONE (2014) ncbi
domestic rabbit monoclonal (C9C5)
  • western blot; human; loading ...; fig 8a
Cell Signaling Technology Shh antibody (Cell Signaling, 2207S) was used in western blot on human samples (fig 8a). Nature (2014) ncbi
domestic rabbit monoclonal (C9C5)
  • immunohistochemistry - paraffin section; human
Cell Signaling Technology Shh antibody (Cell Signaling Technologies, 2207) was used in immunohistochemistry - paraffin section on human samples . Am J Pathol (2012) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (5E1)
  • immunohistochemistry; mouse; 1:5; loading ...; fig 4a
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry on mouse samples at 1:5 (fig 4a). elife (2020) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; mouse; 1:20; loading ...; fig s8b
  • immunohistochemistry - paraffin section; mouse; 1:20; loading ...; fig 6b
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on mouse samples at 1:20 (fig s8b) and in immunohistochemistry - paraffin section on mouse samples at 1:20 (fig 6b). PLoS Genet (2017) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; Common quail; loading ...; fig 6c
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on Common quail samples (fig 6c). Front Cell Dev Biol (2016) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry; chicken; 1:200; fig s2
In order to determine regulation of neural tube closure by modulating tight junctions by cell-cycle-dependent TGFbeta-BMP antagonism, Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry on chicken samples at 1:200 (fig s2). J Cell Sci (2017) ncbi
mouse monoclonal (5E1)
  • western blot; human; 1:500; fig 4b
In order to study human carcinoma-associated mesenchymal stem cells and ovarian cancer chemotherapy through a BMP4/HH signaling loop, Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in western blot on human samples at 1:500 (fig 4b). Oncotarget (2016) ncbi
mouse monoclonal (5E1)
  • immunocytochemistry; mouse; 1:10; fig 3
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunocytochemistry on mouse samples at 1:10 (fig 3). Development (2014) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry - paraffin section; Gallot's lizard; 1:2
In order to study the histogenesis of the lizard visual system, Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry - paraffin section on Gallot's lizard samples at 1:2. J Comp Neurol (2012) ncbi
Articles Reviewed
  1. Liu J, Qian B, Zhou L, Shen G, Tan Y, Liu S, et al. IL25 Enhanced Colitis-Associated Tumorigenesis in Mice by Upregulating Transcription Factor GLI1. Front Immunol. 2022;13:837262 pubmed publisher
  2. Arnold F, Mahaddalkar P, Kraus J, Zhong X, Bergmann W, Srinivasan D, et al. Functional Genomic Screening During Somatic Cell Reprogramming Identifies DKK3 as a Roadblock of Organ Regeneration. Adv Sci (Weinh). 2021;8:2100626 pubmed publisher
  3. Sanchez D, Missiaen R, Skuli N, Steger D, Simon M. Cell-Intrinsic Tumorigenic Functions of PPARγ in Bladder Urothelial Carcinoma. Mol Cancer Res. 2021;19:598-611 pubmed publisher
  4. Ferreira Mendes J, de Faro Valverde L, Torres Andion Vidal M, Paredes B, Coelho P, Allahdadi K, et al. Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma. Int J Mol Sci. 2020;21: pubmed publisher
  5. Matsumoto N, Tanaka S, Horiike T, Shinmyo Y, Kawasaki H. A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain. elife. 2020;9: pubmed publisher
  6. Gigante E, Taylor M, Ivanova A, Kahn R, Caspary T. ARL13B regulates Sonic hedgehog signaling from outside primary cilia. elife. 2020;9: pubmed publisher
  7. Gong P, Li M, Zou C, Tian Q, Xu Z. Tissue Plasminogen Activator Causes Brain Microvascular Endothelial Cell Injury After Oxygen Glucose Deprivation by Inhibiting Sonic Hedgehog Signaling. Neurochem Res. 2019;44:441-449 pubmed publisher
  8. He H, Huang M, Sun S, Wu Y, Lin X. Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development. PLoS Genet. 2017;13:e1006992 pubmed publisher
  9. Matsubara Y, Nakano M, Kawamura K, Tsudzuki M, Funahashi J, Agata K, et al. Inactivation of Sonic Hedgehog Signaling and Polydactyly in Limbs of Hereditary Multiple Malformation, a Novel Type of Talpid Mutant. Front Cell Dev Biol. 2016;4:149 pubmed publisher
  10. Galoian K, Luo S, Qureshi A, Patel P, Price R, Morse A, et al. Effect of cytostatic proline rich polypeptide-1 on tumor suppressors of inflammation pathway signaling in chondrosarcoma. Mol Clin Oncol. 2016;5:618-624 pubmed
  11. Singh V, Singh M, Gorantla S, Poluektova L, Maggirwar S. Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice. Sci Rep. 2016;6:26876 pubmed publisher
  12. Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
  13. Coffman L, Choi Y, McLean K, Allen B, di Magliano M, Buckanovich R. Human carcinoma-associated mesenchymal stem cells promote ovarian cancer chemotherapy resistance via a BMP4/HH signaling loop. Oncotarget. 2016;7:6916-32 pubmed publisher
  14. Wei Q, Chen Z, Wang L, Zhang T, Duan L, Behrens C, et al. LZTFL1 suppresses lung tumorigenesis by maintaining differentiation of lung epithelial cells. Oncogene. 2016;35:2655-63 pubmed publisher
  15. Juhász T, Szentléleky E, Somogyi C, Takács R, Dobrosi N, Engler M, et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015;16:17344-67 pubmed publisher
  16. Hung Y, Chang S, Huang C, Yin J, Hwang C, Yang L, et al. Inhibitor of Differentiation-1 and Hypoxia-Inducible Factor-1 Mediate Sonic Hedgehog Induction by Amyloid Beta-Peptide in Rat Cortical Neurons. Mol Neurobiol. 2016;53:793-809 pubmed publisher
  17. Liu J, Bain L. Arsenic inhibits hedgehog signaling during P19 cell differentiation. Toxicol Appl Pharmacol. 2014;281:243-53 pubmed publisher
  18. So J, Lin J, Wahler J, Liby K, Sporn M, Suh N. A synthetic triterpenoid CDDO-Im inhibits tumorsphere formation by regulating stem cell signaling pathways in triple-negative breast cancer. PLoS ONE. 2014;9:e107616 pubmed publisher
  19. Alfaro A, Roberts B, Kwong L, Bijlsma M, Roelink H. Ptch2 mediates the Shh response in Ptch1-/- cells. Development. 2014;141:3331-9 pubmed publisher
  20. Zahreddine H, Culjkovic Kraljacic B, Assouline S, Gendron P, Romeo A, Morris S, et al. The sonic hedgehog factor GLI1 imparts drug resistance through inducible glucuronidation. Nature. 2014;511:90-3 pubmed publisher
  21. Cigna N, Farrokhi Moshai E, Brayer S, Marchal Sommé J, Wemeau Stervinou L, Fabre A, et al. The hedgehog system machinery controls transforming growth factor-?-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol. 2012;181:2126-37 pubmed publisher
  22. Romero Alemán M, Monzon Mayor M, Santos E, Lang D, Yanes C. Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny. J Comp Neurol. 2012;520:2163-84 pubmed publisher