This is a Validated Antibody Database (VAD) review about mouse Sharpin, based on 8 published articles (read how Labome selects the articles), using Sharpin antibody in all methods. It is aimed to help Labome visitors find the most suited Sharpin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Sharpin synonym: 0610041B22Rik; AW121341; RBCKL1; SIPL1; cpdm; sharpin; mSIPL1; protein kinase C-interacting protein RBCC like 1; shank-associated RH domain-interacting protein; shank-interacting protein-like 1

Knockout validation
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 2b
  • western blot; human; loading ...; fig 3a
In order to discover that Tax recruits linear (Met1-linked) ubiquitin chain assembly complex to the IkB kinase complex, Proteintech Group Sharpin antibody (Proteintech, 14626-1-AP) was used in western blot knockout validation on mouse samples (fig 2b) and in western blot on human samples (fig 3a). PLoS Pathog (2017) ncbi
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 2f
In order to study T cell receptor-induced conjugation of Bcl10 with linear-linked polyubiquitin chains, Proteintech Group Sharpin antibody (Proteintech, 14626-1-AP) was used in western blot knockout validation on human samples (fig 2f). J Biol Chem (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; loading ...; fig s1d
Proteintech Group Sharpin antibody (Proteintech Group, 14626-1-AP) was used in western blot on human samples (fig s1d). Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 6d
  • western blot; human; loading ...; fig 2c
Proteintech Group Sharpin antibody (Proteintech, 14626-1-AP) was used in western blot on mouse samples (fig 6d) and in western blot on human samples (fig 2c). EMBO J (2017) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 2b
  • western blot; human; loading ...; fig 3a
In order to discover that Tax recruits linear (Met1-linked) ubiquitin chain assembly complex to the IkB kinase complex, Proteintech Group Sharpin antibody (Proteintech, 14626-1-AP) was used in western blot knockout validation on mouse samples (fig 2b) and in western blot on human samples (fig 3a). PLoS Pathog (2017) ncbi
rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 2f
In order to study T cell receptor-induced conjugation of Bcl10 with linear-linked polyubiquitin chains, Proteintech Group Sharpin antibody (Proteintech, 14626-1-AP) was used in western blot knockout validation on human samples (fig 2f). J Biol Chem (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig s15
Proteintech Group Sharpin antibody (Proteintech, 14626-1-AP) was used in western blot on human samples at 1:1000 (fig s15). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1
Proteintech Group Sharpin antibody (Proteintech, 14626-I-AP) was used in western blot on human samples (fig 1). Cell Rep (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 6
In order to demonstrate that OTULIN is essential for preventing TNF-associated systemic inflammation in humans and mice, Proteintech Group Sharpin antibody (Proteintech, 14626-1-AP) was used in western blot on mouse samples (fig 6). Cell (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
Proteintech Group Sharpin antibody (ProteinTech, 14626-1-AP) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 2). J Exp Med (2015) ncbi
Articles Reviewed
  1. Strickson S, Emmerich C, Goh E, Zhang J, Kelsall I, MacArtney T, et al. Roles of the TRAF6 and Pellino E3 ligases in MyD88 and RANKL signaling. Proc Natl Acad Sci U S A. 2017;114:E3481-E3489 pubmed publisher
  2. Lafont E, Kantari Mimoun C, Dráber P, De Miguel D, Hartwig T, Reichert M, et al. The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. EMBO J. 2017;36:1147-1166 pubmed publisher
  3. Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, et al. HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains. PLoS Pathog. 2017;13:e1006162 pubmed publisher
  4. Yang Y, Yang C, Chan W, Wang Z, Deibel K, Pomerantz J. Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling. J Biol Chem. 2016;291:25921-25936 pubmed
  5. Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016;7:12547 pubmed publisher
  6. Kupka S, De Miguel D, Dráber P, Martino L, Surinova S, Rittinger K, et al. SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes. Cell Rep. 2016;16:2271-80 pubmed publisher
  7. Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
  8. Boisson B, Laplantine E, Dobbs K, Cobat A, Tarantino N, Hazen M, et al. Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia. J Exp Med. 2015;212:939-51 pubmed publisher