This is a Validated Antibody Database (VAD) review about mouse Sdc4, based on 11 published articles (read how Labome selects the articles), using Sdc4 antibody in all methods. It is aimed to help Labome visitors find the most suited Sdc4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Sdc4 synonym: AA959608; AW108331; Synd4; ryudocan; syndecan-4; syndecan-4; ryudocan core protein

Knockout validation
Abnova
rabbit polyclonal
  • western blot knockout validation; human; 1:1000; fig 3
  • immunocytochemistry; human; fig 1
Abnova Sdc4 antibody (Abnova, PAB9045) was used in western blot knockout validation on human samples at 1:1000 (fig 3) and in immunocytochemistry on human samples (fig 1). Nat Commun (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (5G9)
  • proximity ligation assay; human; loading ...; fig 6b
  • immunocytochemistry; human; loading ...; fig 1
Santa Cruz Biotechnology Sdc4 antibody (Santa Cruz, 5G9) was used in proximity ligation assay on human samples (fig 6b) and in immunocytochemistry on human samples (fig 1). Cell Microbiol (2017) ncbi
mouse monoclonal (5G9)
  • immunocytochemistry; human; 1:40; fig s2b
In order to study the regulation of ADAMTS6 and show that ADAMTS6 reduces heparan sulphate-rich interfaces, Santa Cruz Biotechnology Sdc4 antibody (Santa Cruz, sc-12766) was used in immunocytochemistry on human samples at 1:40 (fig s2b). Sci Rep (2016) ncbi
mouse monoclonal (5G9)
  • flow cytometry; human
  • western blot; human
Santa Cruz Biotechnology Sdc4 antibody (Santa Cruz Biotechnology, 5G9) was used in flow cytometry on human samples and in western blot on human samples . Cell Mol Life Sci (2015) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s10b
In order to report that SDC4 governs flow-induced lymphatic endothelial cell polarization via regulation of VANGL2 expression, Abcam Sdc4 antibody (abcam, ab24511) was used in western blot on mouse samples at 1:1000 (fig s10b). Development (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
In order to study the role of heparan sulfate in corneal epithelial homeostasis and wound healing, Abcam Sdc4 antibody (Abcam, ab24511) was used in immunohistochemistry - paraffin section on mouse samples . Invest Ophthalmol Vis Sci (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
In order to study the role of heparan sulfate in hair follicle and sebaceous gland morphogenesis and homeostasis, Abcam Sdc4 antibody (Abcam, ab24511) was used in immunohistochemistry - paraffin section on mouse samples . J Biol Chem (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:20
In order to study the role of Frizzled-7 in keeping human limbal stem/progenitor cells in an undifferentiated state, Abcam Sdc4 antibody (Abcam, Ab24511) was used in western blot on human samples at 1:20. Stem Cells (2014) ncbi
Abnova
rabbit polyclonal
  • western blot knockout validation; human; 1:1000; fig 3
  • immunocytochemistry; human; fig 1
Abnova Sdc4 antibody (Abnova, PAB9045) was used in western blot knockout validation on human samples at 1:1000 (fig 3) and in immunocytochemistry on human samples (fig 1). Nat Commun (2016) ncbi
rabbit polyclonal
  • flow cytometry; human; loading ...; fig 5
In order to demonstrate that proline/arginine-rich end leucine-rich repeat protein mediates cell adhesion by binding to cell-surface glycosaminoglycans, Abnova Sdc4 antibody (Abnova, PAB9045) was used in flow cytometry on human samples (fig 5). Biochem J (2016) ncbi
BD Biosciences
rat monoclonal (KY/8.2)
  • immunocytochemistry; mouse; 1:21; fig 1
In order to study syndecan-4-mediated podocyte cell-matrix interactions that require N-sulfation of heparan sulfate, BD Biosciences Sdc4 antibody (BD Pharmigen, KY/8.2) was used in immunocytochemistry on mouse samples at 1:21 (fig 1). Am J Physiol Renal Physiol (2016) ncbi
rat monoclonal (KY/8.2)
  • immunoprecipitation; mouse; fig 2
  • western blot; mouse; fig 2
In order to study stretch-activated channels to set cytosolic calcium levels controlled by transmembrane proteoglycans, BD Biosciences Sdc4 antibody (BD Biosciences, KY/8.2) was used in immunoprecipitation on mouse samples (fig 2) and in western blot on mouse samples (fig 2). J Cell Biol (2015) ncbi
Articles Reviewed
  1. Grigorov B, Reungoat E, Gentil dit Maurin A, Varbanov M, Blaising J, Michelet M, et al. Hepatitis C virus infection propagates through interactions between Syndecan-1 and CD81 and impacts the hepatocyte glycocalyx. Cell Microbiol. 2017;19: pubmed publisher
  2. Wang Y, Baeyens N, Corti F, Tanaka K, Fang J, Zhang J, et al. Syndecan 4 controls lymphatic vasculature remodeling during mouse embryonic development. Development. 2016;143:4441-4451 pubmed
  3. Cain S, Mularczyk E, Singh M, Massam Wu T, Kielty C. ADAMTS-10 and -6 differentially regulate cell-cell junctions and focal adhesions. Sci Rep. 2016;6:35956 pubmed publisher
  4. Lin W, Zhang J, Lin H, Li Z, Sun X, Xin D, et al. Syndecan-4 negatively regulates antiviral signalling by mediating RIG-I deubiquitination via CYLD. Nat Commun. 2016;7:11848 pubmed publisher
  5. Sugar T, Wassenhove McCarthy D, Orr A, Green J, van Kuppevelt T, McCarthy K. N-sulfation of heparan sulfate is critical for syndecan-4-mediated podocyte cell-matrix interactions. Am J Physiol Renal Physiol. 2016;310:F1123-35 pubmed publisher
  6. Bengtsson E, Lindblom K, Tillgren V, Aspberg A. The leucine-rich repeat protein PRELP binds fibroblast cell-surface proteoglycans and enhances focal adhesion formation. Biochem J. 2016;473:1153-64 pubmed publisher
  7. Gopal S, Søgaard P, Multhaupt H, Pataki C, Okina E, Xian X, et al. Transmembrane proteoglycans control stretch-activated channels to set cytosolic calcium levels. J Cell Biol. 2015;210:1199-211 pubmed publisher
  8. Coulson Thomas V, Chang S, Yeh L, Coulson Thomas Y, Yamaguchi Y, Esko J, et al. Loss of corneal epithelial heparan sulfate leads to corneal degeneration and impaired wound healing. Invest Ophthalmol Vis Sci. 2015;56:3004-14 pubmed publisher
  9. Pasqualon T, Pruessmeyer J, Weidenfeld S, Babendreyer A, Groth E, Schumacher J, et al. A transmembrane C-terminal fragment of syndecan-1 is generated by the metalloproteinase ADAM17 and promotes lung epithelial tumor cell migration and lung metastasis formation. Cell Mol Life Sci. 2015;72:3783-801 pubmed publisher
  10. Coulson Thomas V, Gesteira T, Esko J, KAO W. Heparan sulfate regulates hair follicle and sebaceous gland morphogenesis and homeostasis. J Biol Chem. 2014;289:25211-26 pubmed publisher
  11. Mei H, Nakatsu M, Baclagon E, Deng S. Frizzled 7 maintains the undifferentiated state of human limbal stem/progenitor cells. Stem Cells. 2014;32:938-45 pubmed publisher