This is a Validated Antibody Database (VAD) review about mouse Rpa1, based on 11 published articles (read how Labome selects the articles), using Rpa1 antibody in all methods. It is aimed to help Labome visitors find the most suited Rpa1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Rpa1 synonym: 5031405K23Rik; 70kDa; AA589576; AW557552; RF-A; RP-A; Rpa; replication protein A 70 kDa DNA-binding subunit; RF-A protein 1; RP-A p70; replication factor A protein 1

Abcam
rabbit monoclonal (EPR3472)
  • immunocytochemistry; human; 1:1000; loading ...; fig 2c
Abcam Rpa1 antibody (Abcam, ab79398) was used in immunocytochemistry on human samples at 1:1000 (fig 2c). Nat Cell Biol (2016) ncbi
rabbit monoclonal (EPR3472)
  • western blot; human; 1:1000; fig s7
Abcam Rpa1 antibody (Abcam, ab79398) was used in western blot on human samples at 1:1000 (fig s7). Nat Commun (2016) ncbi
rabbit monoclonal (EPR3472)
  • western blot; human; fig 3
In order to investigate the role of topoisomerase IIbeta-binding protein 1 in DNA repair and its contribution to cancer, Abcam Rpa1 antibody (Abcam, ab79398) was used in western blot on human samples (fig 3). J Cell Biol (2016) ncbi
rabbit monoclonal (EPR3472)
  • western blot; human; fig 7
In order to learn the association with S phase-specific defects in nucleotide excision repair and mutations in replicative stress response pathways, Abcam Rpa1 antibody (Abcam, ab79398) was used in western blot on human samples (fig 7). J Biol Chem (2016) ncbi
mouse monoclonal (8C3-D12-H10)
  • western blot; rat; 1:80
Abcam Rpa1 antibody (Abcam, ab176467) was used in western blot on rat samples at 1:80. Exp Cell Res (2015) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; fig 4
Bethyl Rpa1 antibody (Bethyl, A300-241A) was used in western blot on human samples (fig 4). Cell Cycle (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 4
In order to determine how PAXX interacts with Ku to promote DNA double-strand break repair, Bethyl Rpa1 antibody (Bethyl Laboratories, A300-241A) was used in western blot on human samples at 1:1000 (fig 4). Science (2015) ncbi
rabbit polyclonal
  • western blot; human
Bethyl Rpa1 antibody (Bethyl, A300?C241A) was used in western blot on human samples . J Biol Chem (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-10)
  • western blot; mouse
Santa Cruz Biotechnology Rpa1 antibody (Santa Cruz Biotechnology, sc-166023) was used in western blot on mouse samples . Nucleic Acids Res (2014) ncbi
mouse monoclonal (H-7)
  • western blot; mouse; 1:500
Santa Cruz Biotechnology Rpa1 antibody (Santa Cruz, sc-48425) was used in western blot on mouse samples at 1:500. J Biol Chem (2012) ncbi
Invitrogen
rabbit polyclonal
  • immunocytochemistry; mouse; fig 1
In order to study why HSC function declines with age, Invitrogen Rpa1 antibody (Thermo Scientific, PA5-21976) was used in immunocytochemistry on mouse samples (fig 1). Nature (2014) ncbi
Articles Reviewed
  1. Hansen R, Mund A, Poulsen S, Sandoval M, Klement K, Tsouroula K, et al. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat Cell Biol. 2016;18:1357-1366 pubmed publisher
  2. Passerini V, Ozeri Galai E, de Pagter M, Donnelly N, Schmalbrock S, Kloosterman W, et al. The presence of extra chromosomes leads to genomic instability. Nat Commun. 2016;7:10754 pubmed publisher
  3. Moudry P, Watanabe K, Wolanin K, Bartkova J, Wassing I, Watanabe S, et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. J Cell Biol. 2016;212:281-8 pubmed publisher
  4. Bélanger F, Angers J, Fortier Ã, Hammond Martel I, Costantino S, Drobetsky E, et al. Mutations in Replicative Stress Response Pathways Are Associated with S Phase-specific Defects in Nucleotide Excision Repair. J Biol Chem. 2016;291:522-37 pubmed publisher
  5. Lindsey Boltz L, Kemp M, Capp C, Sancar A. RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling. Cell Cycle. 2015;14:99-108 pubmed publisher
  6. Ochi T, Blackford A, Coates J, Jhujh S, Mehmood S, Tamura N, et al. DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science. 2015;347:185-188 pubmed publisher
  7. Ghospurkar P, Wilson T, Liu S, Herauf A, Steffes J, Mueller E, et al. Phosphorylation and cellular function of the human Rpa2 N-terminus in the budding yeast Saccharomyces cerevisiae. Exp Cell Res. 2015;331:183-99 pubmed publisher
  8. Witosch J, Wolf E, Mizuno N. Architecture and ssDNA interaction of the Timeless-Tipin-RPA complex. Nucleic Acids Res. 2014;42:12912-27 pubmed publisher
  9. Kemp M, Gaddameedhi S, Choi J, Hu J, Sancar A. DNA repair synthesis and ligation affect the processing of excised oligonucleotides generated by human nucleotide excision repair. J Biol Chem. 2014;289:26574-83 pubmed publisher
  10. Flach J, Bakker S, Mohrin M, Conroy P, Pietras E, Reynaud D, et al. Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells. Nature. 2014;512:198-202 pubmed publisher
  11. Gao Z, Cox J, Gilmore J, Ormsbee B, Mallanna S, Washburn M, et al. Determination of protein interactome of transcription factor Sox2 in embryonic stem cells engineered for inducible expression of four reprogramming factors. J Biol Chem. 2012;287:11384-97 pubmed publisher