This is a Validated Antibody Database (VAD) review about mouse Rock2, based on 20 published articles (read how Labome selects the articles), using Rock2 antibody in all methods. It is aimed to help Labome visitors find the most suited Rock2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Rock2 synonym: B230113H15Rik; ROKalpha; Rho-kinase; Rock-II; Rock2m; mKIAA0619; rho-associated protein kinase 2; Rho-associated coiled-coil forming kinage 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase 2; rho-associated, coiled-coil-containing protein kinase II

Abcam
rabbit polyclonal
  • western blot; mouse; loading ...; fig s10a
In order to explore the role of TPM4 in platelet biogenesis, Abcam Rock2 antibody (Abcam, ab71598) was used in western blot on mouse samples (fig s10a). J Clin Invest (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; loading ...; fig 1c
  • western blot; human; 1:100; loading ...; fig 1d
In order to analyze differential functional effects for rho kinases 1 and 2 at the epithelial zonula adherens, Abcam Rock2 antibody (Abcam, AB71598) was used in immunocytochemistry on human samples at 1:100 (fig 1c) and in western blot on human samples at 1:100 (fig 1d). Mol Biol Cell (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 2l
In order to investigate mechanisms by which synapses are refined, pruned, and stabilized in the prefrontal cortex during adolescence, Abcam Rock2 antibody (Abcam, 71598) was used in western blot on mouse samples at 1:1000 (fig 2l). J Neurosci Res (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:300; fig s4
  • western blot; human; 1:300; fig s4h-i
In order to identify factors that regulate the stability of the RhoA zone, Abcam Rock2 antibody (Abcam, AB71598) was used in immunocytochemistry on human samples at 1:300 (fig s4) and in western blot on human samples at 1:300 (fig s4h-i). Nat Cell Biol (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • western blot; mouse; loading ...; fig 3b
Santa Cruz Biotechnology Rock2 antibody (Santa Cruz, sc-398519) was used in western blot on mouse samples (fig 3b). Nat Immunol (2016) ncbi
mouse monoclonal (30-J)
  • immunohistochemistry - paraffin section; human; 1:500; fig 1
In order to assess the prognostic value of an immunohistochemistry-based three-protein signature model in patients with esophageal squamous cell carcinoma, Santa Cruz Biotechnology Rock2 antibody (Santa Cruz, sc -100425) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1). Oncotarget (2015) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; fig 2b
In order to find compounds that are synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma, Invitrogen Rock2 antibody (Thermo Scientific, PA5-21131) was used in western blot on human samples (fig 2b). Oncogene (2017) ncbi
rabbit polyclonal
  • western blot; human; fig  6
In order to determine if the neurotrophic growth factor signaling pathway was altered in the placenta of selective serotonin reuptake inhibitors-treated, depressed, and healthy mothers, Invitrogen Rock2 antibody (Thermo Scientific, PA5-34895) was used in western blot on human samples (fig  6). PLoS ONE (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D1B1)
  • western blot; mouse; fig 3d
Cell Signaling Technology Rock2 antibody (Cell Signaling, 9029) was used in western blot on mouse samples (fig 3d). FASEB J (2018) ncbi
rabbit monoclonal (D1B1)
  • western blot; mouse; 1:1000; fig s4
In order to propose that epithelial tension and matricellular fibrosis contribute to the aggressiveness of SMAD4 mutant pancreatic tumors, Cell Signaling Technology Rock2 antibody (Cell Signaling, D1B1) was used in western blot on mouse samples at 1:1000 (fig s4). Nat Med (2016) ncbi
rabbit monoclonal (D1B1)
  • western blot; human; fig s2c
Cell Signaling Technology Rock2 antibody (Cell Signaling Technology, 9029) was used in western blot on human samples (fig s2c). Nature (2015) ncbi
rabbit monoclonal (D1B1)
  • western blot; human
In order to evaluate the effect of tumor necrosis factor on claudin-5 endothelial tight junction barriers, Cell Signaling Technology Rock2 antibody (Cell Signaling, 9029) was used in western blot on human samples . PLoS ONE (2015) ncbi
BD Biosciences
mouse monoclonal (21/ROCK-II)
  • western blot; dog; 1:2000; loading ...; fig 6a
In order to investigate that role of zonula occludens-2 in Rho protein activation and epithelial development, BD Biosciences Rock2 antibody (BD Biosciences, 610623) was used in western blot on dog samples at 1:2000 (fig 6a). Biochim Biophys Acta Mol Cell Res (2017) ncbi
mouse monoclonal (21/ROCK-II)
  • western blot; mouse; 1:2000; loading ...; fig 7fs1
In order to test the role of YAP in branching morphogenesis for tissue patterning, BD Biosciences Rock2 antibody (BD Biosciences, 610623) was used in western blot on mouse samples at 1:2000 (fig 7fs1). elife (2017) ncbi
mouse monoclonal (21/ROCK-II)
  • immunohistochemistry - paraffin section; human; loading ...; fig 1a
  • western blot; human; fig 2c
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1f
BD Biosciences Rock2 antibody (BD, 610623) was used in immunohistochemistry - paraffin section on human samples (fig 1a), in western blot on human samples (fig 2c) and in immunohistochemistry - paraffin section on mouse samples (fig 1f). EMBO Mol Med (2017) ncbi
mouse monoclonal (21/ROCK-II)
  • western blot; mouse; 1:2000; loading ...; fig 7a
In order to propose that simvastatin inhibits RhoA/ROCK activity in the chronic hypoxia-exposed lung, BD Biosciences Rock2 antibody (BD Biosciences, 610624) was used in western blot on mouse samples at 1:2000 (fig 7a). Am J Physiol Lung Cell Mol Physiol (2016) ncbi
mouse monoclonal (21/ROCK-II)
  • western blot; human; fig 1
BD Biosciences Rock2 antibody (BD Biosciences, 610624) was used in western blot on human samples (fig 1). Neoplasia (2016) ncbi
mouse monoclonal (21/ROCK-II)
  • western blot; human; 1:300; fig s4h-i
In order to identify factors that regulate the stability of the RhoA zone, BD Biosciences Rock2 antibody (BDBiosciences, 610623) was used in western blot on human samples at 1:300 (fig s4h-i). Nat Cell Biol (2015) ncbi
mouse monoclonal (21/ROCK-II)
  • western blot; mouse; fig 3
BD Biosciences Rock2 antibody (BD transduction laboratories, 21) was used in western blot on mouse samples (fig 3). EMBO Mol Med (2014) ncbi
EMD Millipore
rabbit monoclonal (A9W4)
  • western blot; mouse; 1:1000; loading ...; fig s2e
EMD Millipore Rock2 antibody (Millipore, 04-841) was used in western blot on mouse samples at 1:1000 (fig s2e). Nat Commun (2016) ncbi
rabbit monoclonal (A9W4)
  • western blot; hamsters; 1:500; fig 2
EMD Millipore Rock2 antibody (EMD Millipore, 04-841) was used in western blot on hamsters samples at 1:500 (fig 2). J Cell Biol (2015) ncbi
Articles Reviewed
  1. Khalifeh Soltani A, Gupta D, Ha A, Podolsky M, Datta R, Atabai K. The Mfge8-α8β1-PTEN pathway regulates airway smooth muscle contraction in allergic inflammation. FASEB J. 2018;:fj201800109R pubmed publisher
  2. Raya Sandino A, Castillo Kauil A, Domínguez Calderón A, Alarcón L, Flores Benitez D, Cuellar Perez F, et al. Zonula occludens-2 regulates Rho proteins activity and the development of epithelial cytoarchitecture and barrier function. Biochim Biophys Acta Mol Cell Res. 2017;1864:1714-1733 pubmed publisher
  3. Lin C, Yao E, Zhang K, Jiang X, Croll S, Thompson Peer K, et al. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis. elife. 2017;6: pubmed publisher
  4. Pleines I, Woods J, Chappaz S, Kew V, Foad N, Ballester Beltrán J, et al. Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia. J Clin Invest. 2017;127:814-829 pubmed publisher
  5. Priya R, Liang X, Teo J, Duszyc K, Yap A, Gomez G. ROCK1 but not ROCK2 contributes to RhoA signaling and NMIIA-mediated contractility at the epithelial zonula adherens. Mol Biol Cell. 2017;28:12-20 pubmed publisher
  6. Rath N, Morton J, Julian L, Helbig L, Kadir S, McGhee E, et al. ROCK signaling promotes collagen remodeling to facilitate invasive pancreatic ductal adenocarcinoma tumor cell growth. EMBO Mol Med. 2017;9:198-218 pubmed publisher
  7. Jerić I, Maurer G, Cavallo A, Raguz J, Desideri E, Tarkowski B, et al. A cell-autonomous tumour suppressor role of RAF1 in hepatocarcinogenesis. Nat Commun. 2016;7:13781 pubmed publisher
  8. Thompson J, Nguyen Q, Singh M, Pavesic M, Nesterenko I, Nelson L, et al. Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma. Oncogene. 2017;36:1080-1089 pubmed publisher
  9. Shapiro L, Parsons R, Koleske A, Gourley S. Differential expression of cytoskeletal regulatory factors in the adolescent prefrontal cortex: Implications for cortical development. J Neurosci Res. 2017;95:1123-1143 pubmed publisher
  10. Wong M, Kantores C, Ivanovska J, Jain A, Jankov R. Simvastatin prevents and reverses chronic pulmonary hypertension in newborn rats via pleiotropic inhibition of RhoA signaling. Am J Physiol Lung Cell Mol Physiol. 2016;311:L985-L999 pubmed publisher
  11. Hinsenkamp I, Schulz S, Roscher M, Suhr A, Meyer B, Munteanu B, et al. Inhibition of Rho-Associated Kinase 1/2 Attenuates Tumor Growth in Murine Gastric Cancer. Neoplasia. 2016;18:500-11 pubmed publisher
  12. Park Y, Wood G, Kastner D, Chae J. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS. Nat Immunol. 2016;17:914-21 pubmed publisher
  13. Laklai H, Miroshnikova Y, Pickup M, Collisson E, Kim G, Barrett A, et al. Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression. Nat Med. 2016;22:497-505 pubmed publisher
  14. Pelish H, Liau B, Nitulescu I, Tangpeerachaikul A, Poss Z, Da Silva D, et al. Mediator kinase inhibition further activates super-enhancer-associated genes in AML. Nature. 2015;526:273-276 pubmed publisher
  15. Priya R, Gomez G, Budnar S, Verma S, Cox H, Hamilton N, et al. Feedback regulation through myosin II confers robustness on RhoA signalling at E-cadherin junctions. Nat Cell Biol. 2015;17:1282-93 pubmed publisher
  16. Newell Litwa K, Badoual M, Asmussen H, Patel H, Whitmore L, Horwitz A. ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity. J Cell Biol. 2015;210:225-42 pubmed publisher
  17. Clark P, Kim R, Pober J, Kluger M. Tumor necrosis factor disrupts claudin-5 endothelial tight junction barriers in two distinct NF-κB-dependent phases. PLoS ONE. 2015;10:e0120075 pubmed publisher
  18. Kaihola H, Olivier J, Poromaa I, Ã…kerud H. The effect of antenatal depression and selective serotonin reuptake inhibitor treatment on nerve growth factor signaling in human placenta. PLoS ONE. 2015;10:e0116459 pubmed publisher
  19. Cao H, Zhang S, SHEN J, Wu Z, Wu J, Wang S, et al. A three-protein signature and clinical outcome in esophageal squamous cell carcinoma. Oncotarget. 2015;6:5435-48 pubmed
  20. Arroyo A, Camoletto P, Morando L, Sassoè Pognetto M, Giustetto M, Van Veldhoven P, et al. Pharmacological reversion of sphingomyelin-induced dendritic spine anomalies in a Niemann Pick disease type A mouse model. EMBO Mol Med. 2014;6:398-413 pubmed publisher