This is a Validated Antibody Database (VAD) review about mouse Rock1, based on 22 published articles (read how Labome selects the articles), using Rock1 antibody in all methods. It is aimed to help Labome visitors find the most suited Rock1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Rock1 synonym: 1110055K06Rik; Rock-I; rho-associated protein kinase 1; Rho-associated coiled-coil forming kinase 1; p160 ROCK-1; p160ROCK; rho-associated, coiled-coil-containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase I

Santa Cruz Biotechnology
mouse monoclonal (B-1)
  • western blot; rat; 1:1000; fig 3
Santa Cruz Biotechnology Rock1 antibody (Santa Cruz, sc-374388) was used in western blot on rat samples at 1:1000 (fig 3). Exp Ther Med (2017) ncbi
mouse monoclonal (154C1465)
  • western blot; mouse; 1:1000; loading ...; fig 7a
In order to propose that simvastatin inhibits RhoA/ROCK activity in the chronic hypoxia-exposed lung, Santa Cruz Biotechnology Rock1 antibody (Santa Cruz, SC-52953) was used in western blot on mouse samples at 1:1000 (fig 7a). Am J Physiol Lung Cell Mol Physiol (2016) ncbi
mouse monoclonal (G-6)
  • immunohistochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 3b
Santa Cruz Biotechnology Rock1 antibody (Santa Cruz Biotechnology, sc-17794) was used in immunohistochemistry on human samples (fig 2a) and in western blot on human samples (fig 3b). Oncotarget (2016) ncbi
mouse monoclonal (H-11)
  • western blot; mouse; loading ...; fig 3b
Santa Cruz Biotechnology Rock1 antibody (Santa Cruz, sc-365628) was used in western blot on mouse samples (fig 3b). Nat Immunol (2016) ncbi
mouse monoclonal (154C1465)
  • immunocytochemistry; rat; fig 2
  • western blot; rat; fig 2
Santa Cruz Biotechnology Rock1 antibody (Santa Cruz, sc-52953) was used in immunocytochemistry on rat samples (fig 2) and in western blot on rat samples (fig 2). PLoS ONE (2015) ncbi
mouse monoclonal (G-6)
  • western blot; human; 1:50-500
Santa Cruz Biotechnology Rock1 antibody (Santa Cruz Biotechnology, sc17794) was used in western blot on human samples at 1:50-500. Reprod Biol Endocrinol (2013) ncbi
Abcam
rabbit monoclonal (EP786Y)
  • western blot; rat; loading ...; fig 7
Abcam Rock1 antibody (Abcam, ab45171) was used in western blot on rat samples (fig 7). PLoS ONE (2017) ncbi
rabbit monoclonal (EPR638Y)
  • immunocytochemistry; human; 1:300; loading ...; fig 1b
  • western blot; human; 1:300; loading ...; fig 1d
In order to analyze differential functional effects for rho kinases 1 and 2 at the epithelial zonula adherens, Abcam Rock1 antibody (Abcam, AB134181) was used in immunocytochemistry on human samples at 1:300 (fig 1b) and in western blot on human samples at 1:300 (fig 1d). Mol Biol Cell (2017) ncbi
rabbit monoclonal (EPR638Y)
  • immunocytochemistry; human; 1:300; loading ...; fig 4c
In order to investigate the role of Coronin 1B in regulating the non-muscle myosin II pool, Abcam Rock1 antibody (Abcam, AB134181) was used in immunocytochemistry on human samples at 1:300 (fig 4c). Cell Cycle (2016) ncbi
rabbit monoclonal (EPR638Y)
  • western blot; mouse; loading ...; fig 4d
Abcam Rock1 antibody (Abcam, ab134181) was used in western blot on mouse samples (fig 4d). Int J Mol Sci (2016) ncbi
rabbit monoclonal (EPR638Y)
  • immunocytochemistry; human; 1:300; fig s4
  • western blot; human; 1:300
In order to identify factors that regulate the stability of the RhoA zone, Abcam Rock1 antibody (Abcam, AB134181) was used in immunocytochemistry on human samples at 1:300 (fig s4) and in western blot on human samples at 1:300. Nat Cell Biol (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (C8F7)
  • western blot; mouse; fig 3d
Cell Signaling Technology Rock1 antibody (Cell Signaling, 4035) was used in western blot on mouse samples (fig 3d). FASEB J (2018) ncbi
rabbit monoclonal (C8F7)
  • western blot; mouse; 1:1000; fig s4
In order to propose that epithelial tension and matricellular fibrosis contribute to the aggressiveness of SMAD4 mutant pancreatic tumors, Cell Signaling Technology Rock1 antibody (Cell Signaling, C8F7) was used in western blot on mouse samples at 1:1000 (fig s4). Nat Med (2016) ncbi
rabbit monoclonal (C8F7)
  • western blot; human; fig 7
Cell Signaling Technology Rock1 antibody (Cell Signaling Technology, 4035) was used in western blot on human samples (fig 7). Cell Death Dis (2016) ncbi
rabbit monoclonal (C8F7)
  • western blot; mouse; 1:1000; fig 2a
Cell Signaling Technology Rock1 antibody (Cell Signaling, 4035S) was used in western blot on mouse samples at 1:1000 (fig 2a). Mol Med Rep (2015) ncbi
rabbit monoclonal (C8F7)
  • western blot; human; fig s2c
Cell Signaling Technology Rock1 antibody (Cell Signaling Technology, 4035) was used in western blot on human samples (fig s2c). Nature (2015) ncbi
rabbit monoclonal (C8F7)
  • western blot; mouse
Cell Signaling Technology Rock1 antibody (Cell Signaling, cat#4035) was used in western blot on mouse samples . Mol Neurobiol (2016) ncbi
rabbit monoclonal (C8F7)
  • western blot; human
In order to evaluate the effect of tumor necrosis factor on claudin-5 endothelial tight junction barriers, Cell Signaling Technology Rock1 antibody (Cell Signaling, 4035) was used in western blot on human samples . PLoS ONE (2015) ncbi
BD Biosciences
mouse monoclonal (46/ROCK-I)
  • western blot; human; loading ...; fig 2e
BD Biosciences Rock1 antibody (BD Biosciences, 611137) was used in western blot on human samples (fig 2e). Cell (2019) ncbi
mouse monoclonal (46/ROCK-I)
  • western blot; human; 1:250; loading ...; fig s7c
In order to investigate the effect of actomyosin on cell nuclear morphology and genome stability, BD Biosciences Rock1 antibody (BD Biosciences, 611137) was used in western blot on human samples at 1:250 (fig s7c). Nat Commun (2017) ncbi
mouse monoclonal (46/ROCK-I)
  • western blot; human; fig 2c
BD Biosciences Rock1 antibody (BD, 611136) was used in western blot on human samples (fig 2c). EMBO Mol Med (2017) ncbi
mouse monoclonal (46/ROCK-I)
  • immunocytochemistry; rat; fig 2
  • western blot; rat; fig 2
BD Biosciences Rock1 antibody (BD, 611137) was used in immunocytochemistry on rat samples (fig 2) and in western blot on rat samples (fig 2). PLoS ONE (2015) ncbi
mouse monoclonal (46/ROCK-I)
  • western blot; human; fig 2
BD Biosciences Rock1 antibody (BD Transduction Laboratories, 611137) was used in western blot on human samples (fig 2). Pigment Cell Melanoma Res (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; mouse; fig 2c
EMD Millipore Rock1 antibody (Millipore, 07-1458) was used in western blot on mouse samples (fig 2c). EMBO Mol Med (2017) ncbi
Articles Reviewed
  1. Georgouli M, Herraiz C, Crosas Molist E, Fanshawe B, Maiques O, Perdrix A, et al. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment. Cell. 2019;176:757-774.e23 pubmed publisher
  2. Khalifeh Soltani A, Gupta D, Ha A, Podolsky M, Datta R, Atabai K. The Mfge8-α8β1-PTEN pathway regulates airway smooth muscle contraction in allergic inflammation. FASEB J. 2018;:fj201800109R pubmed publisher
  3. Xu L, Tong R, Li X, Liang L, Huang H. Effects of CASZI on bronchopulmonary development of neonatal rats. Exp Ther Med. 2017;14:6243-6246 pubmed publisher
  4. Takaki T, Montagner M, Serres M, Le Berre M, Russell M, Collinson L, et al. Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability. Nat Commun. 2017;8:16013 pubmed publisher
  5. Zhai L, Liu M, Wang T, Zhang H, Li S, Guo Y. Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury. PLoS ONE. 2017;12:e0174414 pubmed publisher
  6. Priya R, Liang X, Teo J, Duszyc K, Yap A, Gomez G. ROCK1 but not ROCK2 contributes to RhoA signaling and NMIIA-mediated contractility at the epithelial zonula adherens. Mol Biol Cell. 2017;28:12-20 pubmed publisher
  7. Rath N, Morton J, Julian L, Helbig L, Kadir S, McGhee E, et al. ROCK signaling promotes collagen remodeling to facilitate invasive pancreatic ductal adenocarcinoma tumor cell growth. EMBO Mol Med. 2017;9:198-218 pubmed publisher
  8. Wong M, Kantores C, Ivanovska J, Jain A, Jankov R. Simvastatin prevents and reverses chronic pulmonary hypertension in newborn rats via pleiotropic inhibition of RhoA signaling. Am J Physiol Lung Cell Mol Physiol. 2016;311:L985-L999 pubmed publisher
  9. Priya R, Wee K, Budnar S, Gomez G, Yap A, Michael M. Coronin 1B supports RhoA signaling at cell-cell junctions through Myosin II. Cell Cycle. 2016;15:3033-3041 pubmed
  10. Yuan W, Guo Y, Li X, Deng M, Shen Z, Bo C, et al. MicroRNA-126 inhibits colon cancer cell proliferation and invasion by targeting the chemokine (C-X-C motif) receptor 4 and Ras homolog gene family, member A, signaling pathway. Oncotarget. 2016;7:60230-60244 pubmed publisher
  11. Park Y, Wood G, Kastner D, Chae J. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS. Nat Immunol. 2016;17:914-21 pubmed publisher
  12. Laklai H, Miroshnikova Y, Pickup M, Collisson E, Kim G, Barrett A, et al. Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression. Nat Med. 2016;22:497-505 pubmed publisher
  13. Hall A, Lu W, Godfrey J, Antonov A, Paicu C, Moxon S, et al. The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration. Cell Death Dis. 2016;7:e2184 pubmed publisher
  14. Zhang R, Feng X, Zhan M, Huang C, Chen K, Tang X, et al. Transcription Factor Sp1 Promotes the Expression of Porcine ROCK1 Gene. Int J Mol Sci. 2016;17: pubmed publisher
  15. Zhang X, Zhang T, Gao F, Li Q, Shen C, Li Y, et al. Fasudil, a Rho‑kinase inhibitor, prevents intima‑media thickening in a partially ligated carotid artery mouse model: Effects of fasudil in flow‑induced vascular remodeling. Mol Med Rep. 2015;12:7317-25 pubmed publisher
  16. Pelish H, Liau B, Nitulescu I, Tangpeerachaikul A, Poss Z, Da Silva D, et al. Mediator kinase inhibition further activates super-enhancer-associated genes in AML. Nature. 2015;526:273-276 pubmed publisher
  17. Priya R, Gomez G, Budnar S, Verma S, Cox H, Hamilton N, et al. Feedback regulation through myosin II confers robustness on RhoA signalling at E-cadherin junctions. Nat Cell Biol. 2015;17:1282-93 pubmed publisher
  18. Namachivayam K, Mohankumar K, Arbach D, Jagadeeswaran R, Jain S, Natarajan V, et al. All-Trans Retinoic Acid Induces TGF-β2 in Intestinal Epithelial Cells via RhoA- and p38α MAPK-Mediated Activation of the Transcription Factor ATF2. PLoS ONE. 2015;10:e0134003 pubmed publisher
  19. Narayanan K, Chopra V, Rosas H, Malarick K, Hersch S. Rho Kinase Pathway Alterations in the Brain and Leukocytes in Huntington's Disease. Mol Neurobiol. 2016;53:2132-40 pubmed publisher
  20. Clark P, Kim R, Pober J, Kluger M. Tumor necrosis factor disrupts claudin-5 endothelial tight junction barriers in two distinct NF-κB-dependent phases. PLoS ONE. 2015;10:e0120075 pubmed publisher
  21. Vogel C, Smit M, Maddalo G, Possik P, Sparidans R, van der Burg S, et al. Cooperative induction of apoptosis in NRAS mutant melanoma by inhibition of MEK and ROCK. Pigment Cell Melanoma Res. 2015;28:307-17 pubmed publisher
  22. O Brien M, Carbin S, Morrison J, Smith T. Decreased myometrial p160 ROCK-1 expression in obese women at term pregnancy. Reprod Biol Endocrinol. 2013;11:79 pubmed publisher