This is a Validated Antibody Database (VAD) review about mouse Rest, based on 23 published articles (read how Labome selects the articles), using Rest antibody in all methods. It is aimed to help Labome visitors find the most suited Rest antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Rest synonym: 2610008J04Rik; AA407358; NRSF; REST4; RE1-silencing transcription factor; neural-restrictive silencer factor

Abcam
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 5
  • western blot; human; 1:1000; loading ...; fig 2
In order to discover that protein oxidative and glycoxidative damage significantly increases during human brain aging, Abcam Rest antibody (Abcam, ab21635) was used in immunohistochemistry - paraffin section on human samples (fig 5) and in western blot on human samples at 1:1000 (fig 2). Free Radic Biol Med (2017) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; loading ...; fig s5l
In order to demonstrate that iPS-derived cardiomyocytes with a heterozygous GATA4-G296S missense mutation show impaired contractility, calcium handling, and metabolic activity, Invitrogen Rest antibody (Thermo Fisher, PA5-34583) was used in western blot on human samples (fig s5l). Cell (2016) ncbi
EMD Millipore
rabbit polyclonal
  • proximity ligation assay; human; loading ...; fig s4a
  • western blot; human; loading ...; fig 4a
EMD Millipore Rest antibody (Millipore, 07-579) was used in proximity ligation assay on human samples (fig s4a) and in western blot on human samples (fig 4a). Nucleic Acids Res (2018) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 3b
In order to find RE1 silencing transcription factor regulates epithelial-mesenchymal transition and neuroendecrin cell stemness acquisition in hormone-refractory prostate cancer, EMD Millipore Rest antibody (Millipore, 07-579) was used in western blot on human samples (fig 3b). Sci Rep (2017) ncbi
rabbit polyclonal
  • ChIP-Seq; human; loading ...; fig 6a
In order to find RE1 silencing transcription factor regulates epithelial-mesenchymal transition and neuroendecrin cell stemness acquisition in hormone-refractory prostate cancer, EMD Millipore Rest antibody (Millipore, 17-641) was used in ChIP-Seq on human samples (fig 6a). Sci Rep (2017) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; 1:2000; loading ...; fig 2a
  • EMSA; mouse; loading ...; fig 3b
In order to discover a gene silencing mechanism in developing mammalian hearts regulated by the interaction of DNMT3B-mediated non-CpG methylation and REST binding, EMD Millipore Rest antibody (Millipore, 17-641) was used in chromatin immunoprecipitation on mouse samples at 1:2000 (fig 2a) and in EMSA on mouse samples (fig 3b). Nucleic Acids Res (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 4a
EMD Millipore Rest antibody (Millipore, 09-019) was used in western blot on human samples (fig 4a). Oncotarget (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000; fig 2
  • western blot; human; 1:1000; fig 2
In order to analyze regulation of neural progenitor cell differentiation by the miR-20-Rest-Wnt signaling axis, EMD Millipore Rest antibody (Upstate, 07-579) was used in western blot on rat samples at 1:1000 (fig 2) and in western blot on human samples at 1:1000 (fig 2). Sci Rep (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; fig 4
In order to study the regulation of neural gene transcription by RE1-silencing transcription factor optogenetic inhibition, EMD Millipore Rest antibody (Millipore, 17-641) was used in chromatin immunoprecipitation on mouse samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; 2 ug; fig 3
  • western blot; human; fig 3
In order to investigate how loss of RE-1 Silencing Transcription Factor affects breast cancer, EMD Millipore Rest antibody (Millipore, 07-579) was used in chromatin immunoprecipitation on human samples at 2 ug (fig 3) and in western blot on human samples (fig 3). Mol Cell Biol (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; 1:2000
  • western blot; human; 1:2000
EMD Millipore Rest antibody (Millipore, 17-641) was used in chromatin immunoprecipitation on human samples at 1:2000 and in western blot on human samples at 1:2000. Biochim Biophys Acta (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
EMD Millipore Rest antibody (Millipore, 07-579) was used in chromatin immunoprecipitation on human samples . Biochim Biophys Acta (2014) ncbi
rabbit polyclonal
  • western blot; rat
EMD Millipore Rest antibody (Millipore, 07-579) was used in western blot on rat samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
EMD Millipore Rest antibody (Millipore, 07-579) was used in western blot on human samples at 1:1000. Prostate (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
EMD Millipore Rest antibody (Millipore, 07-579) was used in chromatin immunoprecipitation on mouse samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; human
In order to study the involvement of RASSF1A inactivation in carcinogenesis, EMD Millipore Rest antibody (Millipore, 07-579) was used in western blot on human samples . Mol Cell Biol (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; 2-5 ug/ChIP; fig 2a
In order to investigate the role of repressor element 1-silencing transcription factor in neurodegeneration during ageing, EMD Millipore Rest antibody (Millipore, ChIP 17-641) was used in chromatin immunoprecipitation on human samples at 2-5 ug/ChIP (fig 2a). Nature (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
  • western blot; human; fig 1c
In order to investigate the role of repressor element 1-silencing transcription factor in neurodegeneration during ageing, EMD Millipore Rest antibody (Millipore, 07-579) was used in immunocytochemistry on human samples and in western blot on human samples (fig 1c). Nature (2014) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore Rest antibody (Millipore, 09-019) was used in western blot on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • chromatin immunoprecipitation; mouse
EMD Millipore Rest antibody (Millipore, 09-019) was used in chromatin immunoprecipitation on human samples and in chromatin immunoprecipitation on mouse samples . Biochim Biophys Acta (2014) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore Rest antibody (Millipore, 07-579) was used in western blot on human samples . Mol Neurobiol (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • immunoprecipitation; human
EMD Millipore Rest antibody (Millipore, 07-579) was used in chromatin immunoprecipitation on human samples and in immunoprecipitation on human samples . Nucleic Acids Res (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
EMD Millipore Rest antibody (Upstate, 07-579) was used in chromatin immunoprecipitation on human samples . J Neurochem (2013) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
EMD Millipore Rest antibody (Millipore, 07-579) was used in western blot on mouse samples at 1:1000. Am J Physiol Heart Circ Physiol (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; rat; 5 ug
  • western blot; rat; fig 2
EMD Millipore Rest antibody (Millipore, 07-579) was used in chromatin immunoprecipitation on rat samples at 5 ug and in western blot on rat samples (fig 2). Mol Cancer Ther (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; 2 ugs
  • chromatin immunoprecipitation; mouse; 2 ugs
EMD Millipore Rest antibody (Millipore, 17-641) was used in chromatin immunoprecipitation on human samples at 2 ugs and in chromatin immunoprecipitation on mouse samples at 2 ugs. J Biol Chem (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; 2 ug
  • chromatin immunoprecipitation; mouse; 2 ug
EMD Millipore Rest antibody (Millipore, 07-579) was used in chromatin immunoprecipitation on human samples at 2 ug and in chromatin immunoprecipitation on mouse samples at 2 ug. J Biol Chem (2013) ncbi
Articles Reviewed
  1. Lambert M, Terrone S, Giraud G, Benoit Pilven C, Cluet D, Combaret V, et al. The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation. Nucleic Acids Res. 2018;46:7686-7700 pubmed publisher
  2. Chang Y, Lin T, Campbell M, Pan C, Lee S, Lee H, et al. REST is a crucial regulator for acquiring EMT-like and stemness phenotypes in hormone-refractory prostate cancer. Sci Rep. 2017;7:42795 pubmed publisher
  3. Ang Y, Rivas R, Ribeiro A, Srivas R, Rivera J, Stone N, et al. Disease Model of GATA4 Mutation Reveals Transcription Factor Cooperativity in Human Cardiogenesis. Cell. 2016;167:1734-1749.e22 pubmed publisher
  4. Cabre R, Naudi A, Dominguez Gonzalez M, Ayala V, Jove M, Mota Martorell N, et al. Sixty years old is the breakpoint of human frontal cortex aging. Free Radic Biol Med. 2017;103:14-22 pubmed publisher
  5. Zhang D, Wu B, Wang P, Wang Y, Lu P, Nechiporuk T, et al. Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts. Nucleic Acids Res. 2017;45:3102-3115 pubmed publisher
  6. Lin T, Chang Y, Lee S, Campbell M, Wang T, Shen S, et al. REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling. Oncotarget. 2016;7:26137-51 pubmed publisher
  7. Cui Y, Han J, Xiao Z, Chen T, Wang B, Chen B, et al. The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation. Sci Rep. 2016;6:23300 pubmed publisher
  8. Paonessa F, Criscuolo S, Sacchetti S, Amoroso D, Scarongella H, Pecoraro Bisogni F, et al. Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor. Proc Natl Acad Sci U S A. 2016;113:E91-100 pubmed publisher
  9. Meyer K, Albaugh B, Schoenike B, Roopra A. Type 1 Insulin-Like Growth Factor Receptor/Insulin Receptor Substrate 1 Signaling Confers Pathogenic Activity on Breast Tumor Cells Lacking REST. Mol Cell Biol. 2015;35:2991-3004 pubmed publisher
  10. Henriksson R, Bäckman C, Harvey B, Kadyrova H, Bazov I, Shippenberg T, et al. PDYN, a gene implicated in brain/mental disorders, is targeted by REST in the adult human brain. Biochim Biophys Acta. 2014;1839:1226-32 pubmed publisher
  11. Fernandes J, Vieira M, Carreto L, Santos M, Duarte C, Carvalho A, et al. In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons. PLoS ONE. 2014;9:e99958 pubmed publisher
  12. Zhu Y, Liu C, Cui Y, Nadiminty N, Lou W, Gao A. Interleukin-6 induces neuroendocrine differentiation (NED) through suppression of RE-1 silencing transcription factor (REST). Prostate. 2014;74:1086-94 pubmed publisher
  13. Seki M, Masaki H, Arauchi T, Nakauchi H, Sugano S, Suzuki Y. A comparison of the rest complex binding patterns in embryonic stem cells and epiblast stem cells. PLoS ONE. 2014;9:e95374 pubmed publisher
  14. Ram R, Mendiratta S, Bodemann B, Torres M, Eskiocak U, White M. RASSF1A inactivation unleashes a tumor suppressor/oncogene cascade with context-dependent consequences on cell cycle progression. Mol Cell Biol. 2014;34:2350-8 pubmed publisher
  15. Lu T, Aron L, Zullo J, Pan Y, Kim H, Chen Y, et al. REST and stress resistance in ageing and Alzheimer's disease. Nature. 2014;507:448-54 pubmed publisher
  16. Chang P, Wang T, Chang Y, Chu C, Lee C, Hsu H, et al. Autophagy pathway is required for IL-6 induced neuroendocrine differentiation and chemoresistance of prostate cancer LNCaP cells. PLoS ONE. 2014;9:e88556 pubmed publisher
  17. Bersten D, Wright J, McCarthy P, Whitelaw M. Regulation of the neuronal transcription factor NPAS4 by REST and microRNAs. Biochim Biophys Acta. 2014;1839:13-24 pubmed publisher
  18. Di Carlo V, Grossi E, Laneve P, Morlando M, Dini Modigliani S, Ballarino M, et al. TDP-43 regulates the microprocessor complex activity during in vitro neuronal differentiation. Mol Neurobiol. 2013;48:952-63 pubmed publisher
  19. Doghman M, Figueiredo B, Volante M, Papotti M, Lalli E. Integrative analysis of SF-1 transcription factor dosage impact on genome-wide binding and gene expression regulation. Nucleic Acids Res. 2013;41:8896-907 pubmed publisher
  20. Conforti P, Zuccato C, Gaudenzi G, Ieraci A, Camnasio S, Buckley N, et al. Binding of the repressor complex REST-mSIN3b by small molecules restores neuronal gene transcription in Huntington's disease models. J Neurochem. 2013;127:22-35 pubmed publisher
  21. Medford H, Porter K, Marsh S. Immediate effects of a single exercise bout on protein O-GlcNAcylation and chromatin regulation of cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2013;305:H114-23 pubmed publisher
  22. Amalraj J, Cutler S, Ghazawi I, Boyle G, Ralph S. REST negatively and ISGF3 positively regulate the human STAT1 gene in melanoma. Mol Cancer Ther. 2013;12:1288-98 pubmed publisher
  23. Paonessa F, Latifi S, Scarongella H, Cesca F, Benfenati F. Specificity protein 1 (Sp1)-dependent activation of the synapsin I gene (SYN1) is modulated by RE1-silencing transcription factor (REST) and 5'-cytosine-phosphoguanine (CpG) methylation. J Biol Chem. 2013;288:3227-39 pubmed publisher