This is a Validated Antibody Database (VAD) review about mouse Rbpj, based on 22 published articles (read how Labome selects the articles), using Rbpj antibody in all methods. It is aimed to help Labome visitors find the most suited Rbpj antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Rbpj synonym: AI843960; CBF1; Igkjrb; Igkrsbp; RBP-J; RBP-J kappa; RBP-Jkappa; RBPjk; Rbpsuh; recombining binding protein suppressor of hairless; J-kappa recombination signal binding protein; Jkappa recombination signal binding protein

Knockout validation
Cosmo Bio
rat monoclonal (K0043)
  • western blot knockout validation; mouse; 1:100; fig 2c
  • immunohistochemistry - frozen section; mouse; 1:100; fig 2a
Cosmo Bio Rbpj antibody (CosmoBio, SIM-2ZRBP-1) was used in western blot knockout validation on mouse samples at 1:100 (fig 2c) and in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 2a). Sci Rep (2018) ncbi
Cosmo Bio
rat monoclonal (T6709)
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig ed5a
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 3e
Cosmo Bio Rbpj antibody (Cosmobio, SIM-2ZRBP2) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig ed5a) and in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 3e). Nature (2015) ncbi
Abcam
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4d
  • western blot; human; fig 7e
Abcam Rbpj antibody (Abcam, AB25949) was used in chromatin immunoprecipitation on human samples (fig 4d) and in western blot on human samples (fig 7e). J Virol (2017) ncbi
rabbit monoclonal (EPR13479)
  • immunocytochemistry; rat; 1:100; loading ...; fig 5d
  • immunohistochemistry; rat; 1:100; loading ...; fig 2d
  • western blot; rat; 1:500; loading ...; fig 2a
In order to determine if hypertonic saline induces Notch signaling to regulate the production of proinflammatory mediators, Abcam Rbpj antibody (Abcam, ab180588) was used in immunocytochemistry on rat samples at 1:100 (fig 5d), in immunohistochemistry on rat samples at 1:100 (fig 2d) and in western blot on rat samples at 1:500 (fig 2a). BMC Neurosci (2017) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 5a
Abcam Rbpj antibody (abcam, ab25949) was used in chromatin immunoprecipitation on human samples (fig 5a). Nat Commun (2016) ncbi
rabbit polyclonal
  • ChIP-Seq; human; fig 3
  • western blot; human
Abcam Rbpj antibody (Abcam, AB25949) was used in ChIP-Seq on human samples (fig 3) and in western blot on human samples . PLoS Pathog (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
Abcam Rbpj antibody (Abcam, ab25949) was used in chromatin immunoprecipitation on mouse samples . Leukemia (2014) ncbi
rabbit polyclonal
  • western blot; mouse
Abcam Rbpj antibody (Abcam, ab25949) was used in western blot on mouse samples . J Biol Chem (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; 1:200
In order to investigate the role of Notch signaling in the maintenance of conjunctival epithelial identity, Abcam Rbpj antibody (Abcam, Ab25949) was used in chromatin immunoprecipitation on mouse samples at 1:200. Development (2013) ncbi
Cell Signaling Technology
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
Cell Signaling Technology Rbpj antibody (Cell Signaling, 5442S) was used in immunohistochemistry - frozen section on mouse samples (fig 1a). J Clin Invest (2019) ncbi
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig s5a
  • western blot; human; loading ...; fig s5a
Cell Signaling Technology Rbpj antibody (Cell Signaling, 5442) was used in immunoprecipitation on human samples (fig s5a) and in western blot on human samples (fig s5a). J Clin Invest (2018) ncbi
rabbit monoclonal (D10A4)
  • immunohistochemistry; mouse; loading ...; fig 2t
Cell Signaling Technology Rbpj antibody (Cell Signaling, 5313) was used in immunohistochemistry on mouse samples (fig 2t). Sci Rep (2017) ncbi
rabbit monoclonal (D10A4)
  • chromatin immunoprecipitation; human; loading ...; fig 5a
Cell Signaling Technology Rbpj antibody (Cell signaling, 5313s) was used in chromatin immunoprecipitation on human samples (fig 5a). Nat Commun (2016) ncbi
rabbit monoclonal (D10A4)
  • western blot; human; 1:800; loading ...; fig 3a
Cell Signaling Technology Rbpj antibody (Cell Signaling, 5313) was used in western blot on human samples at 1:800 (fig 3a). Oncotarget (2016) ncbi
rabbit monoclonal (D10A4)
  • chromatin immunoprecipitation; mouse; fig 7d
Cell Signaling Technology Rbpj antibody (Cell Signaling, 5313) was used in chromatin immunoprecipitation on mouse samples (fig 7d). Nat Commun (2016) ncbi
rabbit monoclonal (D10A4)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 6b
  • western blot; mouse; loading ...; fig s1
Cell Signaling Technology Rbpj antibody (Cell Signaling, 5313) was used in immunohistochemistry - paraffin section on mouse samples (fig 6b) and in western blot on mouse samples (fig s1). J Clin Invest (2016) ncbi
rabbit monoclonal (D10A4)
  • western blot; mouse; 1:1000; fig 2
In order to define transcriptional, metabolic ,and ubiquitin-cycling nodes controlled by Rbpj, Pfkl and Rnf145 during the phagocyte oxidative burst, Cell Signaling Technology Rbpj antibody (Cell Signalling Technology, 5313S) was used in western blot on mouse samples at 1:1000 (fig 2). Nat Commun (2015) ncbi
rabbit monoclonal (D10A4)
  • immunohistochemistry; mouse; 1:500
  • western blot; mouse; 1:1000
In order to study the role of Notch-dependent erroneous cell cycle reentry in neurodegeneration, Cell Signaling Technology Rbpj antibody (Cell Signaling, 5313) was used in immunohistochemistry on mouse samples at 1:500 and in western blot on mouse samples at 1:1000. Cell Death Differ (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:500
In order to investigate the signalling pathways involved in the development of invasive trophoblasts, Cell Signaling Technology Rbpj antibody (Cell Signalling, 5442) was used in western blot on human samples at 1:500. Mol Hum Reprod (2014) ncbi
rabbit monoclonal (D10A4)
  • chromatin immunoprecipitation; human
Cell Signaling Technology Rbpj antibody (Cell Signaling, 5313) was used in chromatin immunoprecipitation on human samples . PLoS ONE (2013) ncbi
rabbit polyclonal
  • western blot; mouse
  • western blot; human; fig 4
Cell Signaling Technology Rbpj antibody (Cell Signaling Technology, 5442) was used in western blot on mouse samples and in western blot on human samples (fig 4). J Biol Chem (2013) ncbi
Cosmo Bio
rat monoclonal (K0043)
  • western blot knockout validation; mouse; 1:100; fig 2c
  • immunohistochemistry - frozen section; mouse; 1:100; fig 2a
Cosmo Bio Rbpj antibody (CosmoBio, SIM-2ZRBP-1) was used in western blot knockout validation on mouse samples at 1:100 (fig 2c) and in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 2a). Sci Rep (2018) ncbi
rat monoclonal (T6709)
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig ed5a
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 3e
Cosmo Bio Rbpj antibody (Cosmobio, SIM-2ZRBP2) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig ed5a) and in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 3e). Nature (2015) ncbi
rat monoclonal (T6709)
  • western blot; human; 1:500
  • western blot; mouse; 1:500
In order to identify proteins associated with EBNA3A, EBNA3B, and EBNA3C in lymphoblastoid cells, Cosmo Bio Rbpj antibody (Cosmo Bio Co, Hyb-T6709) was used in western blot on human samples at 1:500 and in western blot on mouse samples at 1:500. PLoS Pathog (2015) ncbi
rat monoclonal (T6709)
  • immunohistochemistry; geckos; 1:500; loading ...
  • immunohistochemistry; Chinese soft-shelled turtle; 1:500
Cosmo Bio Rbpj antibody (Cosmo Bio, SIM-2ZRBP2) was used in immunohistochemistry on geckos samples at 1:500 and in immunohistochemistry on Chinese soft-shelled turtle samples at 1:500. Front Neurosci (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4o
EMD Millipore Rbpj antibody (Millipore, AB2284) was used in western blot on mouse samples at 1:1000 (fig 4o). Genes Dev (2017) ncbi
Articles Reviewed
  1. Bartolomé A, Zhu C, Sussel L, Pajvani U. Notch signaling dynamically regulates adult β cell proliferation and maturity. J Clin Invest. 2019;129:268-280 pubmed publisher
  2. Li L, Guturi K, Gautreau B, Patel P, Saad A, Morii M, et al. Ubiquitin ligase RNF8 suppresses Notch signaling to regulate mammary development and tumorigenesis. J Clin Invest. 2018;128:4525-4542 pubmed publisher
  3. Miesfeld J, Moon M, Riesenberg A, Contreras A, Kovall R, Brown N. Rbpj direct regulation of Atoh7 transcription in the embryonic mouse retina. Sci Rep. 2018;8:10195 pubmed publisher
  4. Nakagawa N, Li J, Yabuno Nakagawa K, Eom T, Cowles M, Mapp T, et al. APC sets the Wnt tone necessary for cerebral cortical progenitor development. Genes Dev. 2017;31:1679-1692 pubmed publisher
  5. Lu F, Wiedmer A, Martin K, Wickramasinghe P, Kossenkov A, Lieberman P. Coordinate Regulation of TET2 and EBNA2 Controls the DNA Methylation State of Latent Epstein-Barr Virus. J Virol. 2017;91: pubmed publisher
  6. Watanabe Y, Miyasaka K, Kubo A, Kida Y, Nakagawa O, Hirate Y, et al. Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm. Sci Rep. 2017;7:46135 pubmed publisher
  7. Zeng W, Han Y, Zhu G, Huang L, Deng Y, Wang Q, et al. Hypertonic saline attenuates expression of Notch signaling and proinflammatory mediators in activated microglia in experimentally induced cerebral ischemia and hypoxic BV-2 microglia. BMC Neurosci. 2017;18:32 pubmed publisher
  8. Mouillesseaux K, Wiley D, Saunders L, Wylie L, Kushner E, Chong D, et al. Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6. Nat Commun. 2016;7:13247 pubmed publisher
  9. Asnaghi L, Tripathy A, Yang Q, Kaur H, Hanaford A, Yu W, et al. Targeting Notch signaling as a novel therapy for retinoblastoma. Oncotarget. 2016;7:70028-70044 pubmed publisher
  10. Klein Hessling S, Rudolf R, Muhammad K, Knobeloch K, Maqbool M, Cauchy P, et al. A threshold level of NFATc1 activity facilitates thymocyte differentiation and opposes notch-driven leukaemia development. Nat Commun. 2016;7:11841 pubmed publisher
  11. Wang C, Inzana J, Mirando A, Ren Y, Liu Z, Shen J, et al. NOTCH signaling in skeletal progenitors is critical for fracture repair. J Clin Invest. 2016;126:1471-81 pubmed publisher
  12. Lu F, Chen H, Kossenkov A, DeWispeleare K, Won K, Lieberman P. EBNA2 Drives Formation of New Chromosome Binding Sites and Target Genes for B-Cell Master Regulatory Transcription Factors RBP-jκ and EBF1. PLoS Pathog. 2016;12:e1005339 pubmed publisher
  13. Graham D, Becker C, Doan A, Goel G, Villablanca E, Knights D, et al. Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst. Nat Commun. 2015;6:7838 pubmed publisher
  14. Pardo Saganta A, Tata P, Law B, Saez B, Chow R, Prabhu M, et al. Parent stem cells can serve as niches for their daughter cells. Nature. 2015;523:597-601 pubmed publisher
  15. Ohashi M, Holthaus A, Calderwood M, Lai C, Krastins B, Sarracino D, et al. The EBNA3 family of Epstein-Barr virus nuclear proteins associates with the USP46/USP12 deubiquitination complexes to regulate lymphoblastoid cell line growth. PLoS Pathog. 2015;11:e1004822 pubmed publisher
  16. Marathe S, Liu S, Brai E, Kaczarowski M, Alberi L. Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry. Cell Death Differ. 2015;22:1775-84 pubmed publisher
  17. Nomura T, Yamashita W, Gotoh H, Ono K. Genetic manipulation of reptilian embryos: toward an understanding of cortical development and evolution. Front Neurosci. 2015;9:45 pubmed publisher
  18. Velicky P, Haider S, Otti G, Fiala C, Pollheimer J, Knöfler M. Notch-dependent RBPJ? inhibits proliferation of human cytotrophoblasts and their differentiation into extravillous trophoblasts. Mol Hum Reprod. 2014;20:756-66 pubmed publisher
  19. Kumar V, Palermo R, Talora C, Campese A, Checquolo S, Bellavia D, et al. Notch and NF-kB signaling pathways regulate miR-223/FBXW7 axis in T-cell acute lymphoblastic leukemia. Leukemia. 2014;28:2324-35 pubmed publisher
  20. Guo L, Chen H, Li Y, Zhou Q, Sui Y. An aquaporin 3-notch1 axis in keratinocyte differentiation and inflammation. PLoS ONE. 2013;8:e80179 pubmed publisher
  21. Larabee J, Shakir S, Barua S, Ballard J. Increased cAMP in monocytes augments Notch signaling mechanisms by elevating RBP-J and transducin-like enhancer of Split (TLE). J Biol Chem. 2013;288:21526-36 pubmed publisher
  22. Zhang Y, Lam O, Nguyen M, Ng G, Pear W, Ai W, et al. Mastermind-like transcriptional co-activator-mediated Notch signaling is indispensable for maintaining conjunctival epithelial identity. Development. 2013;140:594-605 pubmed publisher