This is a Validated Antibody Database (VAD) review about mouse Rab8a, based on 22 published articles (read how Labome selects the articles), using Rab8a antibody in all methods. It is aimed to help Labome visitors find the most suited Rab8a antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Rab8a synonym: AA409338; Mel; ras-related protein Rab-8A; cell line NK14 derived transforming oncogene; oncogene c-mel

Knockout validation
Abcam
rabbit monoclonal (EPR14873)
  • western blot knockout validation; mouse; loading ...; fig 5a
Abcam Rab8a antibody (Abcam, ab188574) was used in western blot knockout validation on mouse samples (fig 5a). Cell Rep (2017) ncbi
Abcam
rabbit monoclonal (EPR14873)
  • western blot knockout validation; mouse; loading ...; fig 5a
Abcam Rab8a antibody (Abcam, ab188574) was used in western blot knockout validation on mouse samples (fig 5a). Cell Rep (2017) ncbi
rabbit monoclonal (EPR14873)
  • western blot; mouse; 1:1000; fig 5s1
In order to report the mechanism by which thrombospondin-4 regulates skeletal muscle integrity, Abcam Rab8a antibody (Abcam, ab188574) was used in western blot on mouse samples at 1:1000 (fig 5s1). elife (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; fig 4b
Proteintech Group Rab8a antibody (Proteintech, 55296-1-AP) was used in western blot on human samples (fig 4b). Hum Mol Genet (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; loading ...; fig 7d
Proteintech Group Rab8a antibody (Proteintech, 55296-1-AP) was used in immunocytochemistry on mouse samples at 1:200 (fig 7d). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:250; fig s5
Proteintech Group Rab8a antibody (Proteintech, 55296-1-AP) was used in immunohistochemistry - paraffin section on mouse samples at 1:250 (fig s5). Nat Commun (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (D22D8)
  • western blot; human; loading ...; fig 2e
Cell Signaling Technology Rab8a antibody (Cell Signaling, 6975) was used in western blot on human samples (fig 2e). elife (2017) ncbi
rabbit monoclonal (D22D8)
  • western blot; mouse; loading ...; fig 6b
Cell Signaling Technology Rab8a antibody (Cell Signaling, 6975) was used in western blot on mouse samples (fig 6b). Proc Natl Acad Sci U S A (2017) ncbi
rabbit monoclonal (D22D8)
  • western blot; mouse; 1:5000; loading ...; fig 5f
Cell Signaling Technology Rab8a antibody (Cell Signaling Technology, 6975) was used in western blot on mouse samples at 1:5000 (fig 5f). Autophagy (2016) ncbi
rabbit monoclonal (D22D8)
  • western blot; rat; 1:1000; fig 1
In order to assess how rabphilin 3A retains NMDA receptors through interaction with GluN2A/PSD-95 complex synapse sites, Cell Signaling Technology Rab8a antibody (Cell Signaling, D22D8) was used in western blot on rat samples at 1:1000 (fig 1). Nat Commun (2015) ncbi
rabbit monoclonal (D22D8)
  • western blot; human
In order to investigate the role of Apaf1 in axonogenesis, Cell Signaling Technology Rab8a antibody (Cell Signaling, D22D8) was used in western blot on human samples . Cell Mol Life Sci (2015) ncbi
rabbit monoclonal (D22D8)
  • immunocytochemistry; mouse
In order to study the intracellular role of sorcin, Cell Signaling Technology Rab8a antibody (Cell Signaling, D22D8) was used in immunocytochemistry on mouse samples . PLoS ONE (2014) ncbi
BD Biosciences
mouse monoclonal (4/Rab8)
  • immunohistochemistry - frozen section; mouse; 1:200; fig 4h
BD Biosciences Rab8a antibody (BD Biosciences, 610844) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 4h). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (4/Rab8)
  • western blot; human; 1:1000; loading ...; fig s1e
In order to investigate the interaction between GRAF1 and RAB8, BD Biosciences Rab8a antibody (BD Biosciences, 610844) was used in western blot on human samples at 1:1000 (fig s1e). J Cell Sci (2017) ncbi
mouse monoclonal (4/Rab8)
  • western blot; human; 1:1000; loading ...; fig 6b
BD Biosciences Rab8a antibody (BD Bioscience, 610844) was used in western blot on human samples at 1:1000 (fig 6b). J Cell Sci (2016) ncbi
mouse monoclonal (4/Rab8)
  • immunocytochemistry; dog; 1:100; loading ...; fig 1d
In order to study primary ciliogenesis in polarized epithelial cells, BD Biosciences Rab8a antibody (BD, 610844) was used in immunocytochemistry on dog samples at 1:100 (fig 1d). J Cell Biol (2016) ncbi
mouse monoclonal (4/Rab8)
  • western blot; mouse; 1:1000; loading ...; fig 1d
BD Biosciences Rab8a antibody (BD Biosciences, 610844) was used in western blot on mouse samples at 1:1000 (fig 1d). Diabetes (2016) ncbi
mouse monoclonal (4/Rab8)
  • immunocytochemistry; rat; fig 2
  • western blot; mouse; fig 1
In order to investigate the contribution of several Rabs to neurite outgrowth, BD Biosciences Rab8a antibody (BD Biosciences, 610845) was used in immunocytochemistry on rat samples (fig 2) and in western blot on mouse samples (fig 1). Mol Biol Cell (2016) ncbi
mouse monoclonal (4/Rab8)
  • western blot; human; fig 1
In order to determine how E3 ligase tethering by PCM1 promotes ciliogenesis and controls the abundance of centrosomal KIAA0586/Talpid3, BD Biosciences Rab8a antibody (BD Biosciences, 610844) was used in western blot on human samples (fig 1). elife (2016) ncbi
mouse monoclonal (4/Rab8)
  • immunocytochemistry; dog
In order to study the role of MAL, a tetraspanning protein, in primary cilium formation, BD Biosciences Rab8a antibody (BD Transduction Laboratories, 610844) was used in immunocytochemistry on dog samples . J Cell Sci (2015) ncbi
mouse monoclonal (4/Rab8)
  • western blot; human; fig 4
In order to discover that Myosin Vc contributes to melanosome biogenesis via Rab GTPases, BD Biosciences Rab8a antibody (BD Biosciences, 610845) was used in western blot on human samples (fig 4). J Biol Chem (2014) ncbi
mouse monoclonal (4/Rab8)
  • western blot; mouse; fig 2
In order to examiine how Rab8a and PI3Kgamma regulate TLR4 signaling, BD Biosciences Rab8a antibody (BD Transduction, 610845) was used in western blot on mouse samples (fig 2). Nat Commun (2014) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunocytochemistry; human; 1:100; loading ...; fig s1a
Sigma-Aldrich Rab8a antibody (Sigma, R5530) was used in immunocytochemistry on human samples at 1:100 (fig s1a). Biochem Biophys Res Commun (2018) ncbi
rabbit polyclonal
  • western blot; dog; 1:500; loading ...; fig s4a
In order to study primary ciliogenesis in polarized epithelial cells, Sigma-Aldrich Rab8a antibody (Sigma, R5530) was used in western blot on dog samples at 1:500 (fig s4a). J Cell Biol (2016) ncbi
Articles Reviewed
  1. Jiu Y. Vimentin intermediate filaments function as a physical barrier during intracellular trafficking of caveolin-1. Biochem Biophys Res Commun. 2018;507:161-167 pubmed publisher
  2. Steger M, Diez F, Dhekne H, Lis P, Nirujogi R, Karayel Ö, et al. Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis. elife. 2017;6: pubmed publisher
  3. Maurya D, Bohm S, Alenius M. Hedgehog signaling regulates ciliary localization of mouse odorant receptors. Proc Natl Acad Sci U S A. 2017;114:E9386-E9394 pubmed publisher
  4. Gulbranson D, Davis E, Demmitt B, Ouyang Y, Ye Y, Yu H, et al. RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis. Proc Natl Acad Sci U S A. 2017;114:E8224-E8233 pubmed publisher
  5. Vidal Quadras M, Holst M, Francis M, Larsson E, Hachimi M, Yau W, et al. Endocytic turnover of Rab8 controls cell polarization. J Cell Sci. 2017;130:1147-1157 pubmed publisher
  6. Kim M, Deng H, Wong Y, Siddique T, Krainc D. The Parkinson's disease-linked protein TMEM230 is required for Rab8a-mediated secretory vesicle trafficking and retromer trafficking. Hum Mol Genet. 2017;26:729-741 pubmed publisher
  7. Burikhanov R, Hebbar N, Noothi S, Shukla N, Sledziona J, Araujo N, et al. Chloroquine-Inducible Par-4 Secretion Is Essential for Tumor Cell Apoptosis and Inhibition of Metastasis. Cell Rep. 2017;18:508-519 pubmed publisher
  8. Yu F, Sharma S, Skowronek A, Erdmann K. The serologically defined colon cancer antigen-3 (SDCCAG3) is involved in the regulation of ciliogenesis. Sci Rep. 2016;6:35399 pubmed publisher
  9. Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
  10. Madugula V, Lu L. A ternary complex comprising transportin1, Rab8 and the ciliary targeting signal directs proteins to ciliary membranes. J Cell Sci. 2016;129:3922-3934 pubmed
  11. Bernabé Rubio M, Andrés G, Casares Arias J, Fernández Barrera J, Rangel L, Reglero Real N, et al. Novel role for the midbody in primary ciliogenesis by polarized epithelial cells. J Cell Biol. 2016;214:259-73 pubmed publisher
  12. Vazirani R, Verma A, Sadacca L, Buckman M, Picatoste B, Beg M, et al. Disruption of Adipose Rab10-Dependent Insulin Signaling Causes Hepatic Insulin Resistance. Diabetes. 2016;65:1577-89 pubmed publisher
  13. Homma Y, Fukuda M. Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol Biol Cell. 2016;27:2107-18 pubmed publisher
  14. Wang L, Lee K, Malonis R, SANCHEZ I, Dynlacht B. Tethering of an E3 ligase by PCM1 regulates the abundance of centrosomal KIAA0586/Talpid3 and promotes ciliogenesis. elife. 2016;5: pubmed publisher
  15. Son S, Cha M, Choi H, Kang S, Choi H, Lee M, et al. Insulin-degrading enzyme secretion from astrocytes is mediated by an autophagy-based unconventional secretory pathway in Alzheimer disease. Autophagy. 2016;12:784-800 pubmed publisher
  16. Stanic J, Carta M, Eberini I, Pelucchi S, Marcello E, Genazzani A, et al. Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex. Nat Commun. 2015;6:10181 pubmed publisher
  17. De Zio D, Molinari F, Rizza S, Gatta L, Ciotti M, Salvatore A, et al. Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth. Cell Mol Life Sci. 2015;72:4173-91 pubmed publisher
  18. Reales E, Bernabé Rubio M, Casares Arias J, Rentero C, Fernández Barrera J, Rangel L, et al. The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation. J Cell Sci. 2015;128:2261-70 pubmed publisher
  19. Bultema J, Boyle J, Malenke P, Martin F, Dell Angelica E, Cheney R, et al. Myosin vc interacts with Rab32 and Rab38 proteins and works in the biogenesis and secretion of melanosomes. J Biol Chem. 2014;289:33513-28 pubmed publisher
  20. Luo L, Wall A, Yeo J, Condon N, Norwood S, Schoenwaelder S, et al. Rab8a interacts directly with PI3K? to modulate TLR4-driven PI3K and mTOR signalling. Nat Commun. 2014;5:4407 pubmed publisher
  21. Veleri S, Manjunath S, Fariss R, May Simera H, Brooks M, Foskett T, et al. Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis. Nat Commun. 2014;5:4207 pubmed publisher
  22. Lalioti V, Ilari A, O Connell D, Poser E, Sandoval I, Colotti G. Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis. PLoS ONE. 2014;9:e85438 pubmed publisher