This is a Validated Antibody Database (VAD) review about mouse Pycard, based on 6 published articles (read how Labome selects the articles), using Pycard antibody in all methods. It is aimed to help Labome visitors find the most suited Pycard antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Pycard synonym: 9130417A21Rik; Asc; CARD5; TMS-1; TNS1; masc

Knockout validation
MBL International
  • western blot knockout validation; human; loading ...; fig s1b
In order to report a role for MLKL in NLRP3 inflammasome activation, MBL International Pycard antibody (MBL International, D086-3) was used in western blot knockout validation on human samples (fig s1b). J Immunol (2017) ncbi
Novus Biologicals
domestic rabbit polyclonal
Novus Biologicals Pycard antibody (Novus Biologicals, NBP1-78977) was used . PLoS ONE (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D2W8U)
  • immunocytochemistry; mouse; 1:800; loading ...; fig 4d
  • western blot; mouse; 1:1000; loading ...; fig 2c
Cell Signaling Technology Pycard antibody (Cell Signaling Technology, 67824) was used in immunocytochemistry on mouse samples at 1:800 (fig 4d) and in western blot on mouse samples at 1:1000 (fig 2c). Cell Death Dis (2020) ncbi
domestic rabbit monoclonal (D2W8U)
  • western blot; mouse; loading ...; fig 2s1b
Cell Signaling Technology Pycard antibody (Cell Signaling, 67824) was used in western blot on mouse samples (fig 2s1b). elife (2019) ncbi
domestic rabbit monoclonal (D2W8U)
  • western blot; mouse; 1:1000; loading ...; fig 2c
Cell Signaling Technology Pycard antibody (Cell Signaling, 67824) was used in western blot on mouse samples at 1:1000 (fig 2c). Cell Death Dis (2018) ncbi
MBL International
  • western blot knockout validation; human; loading ...; fig s1b
In order to report a role for MLKL in NLRP3 inflammasome activation, MBL International Pycard antibody (MBL International, D086-3) was used in western blot knockout validation on human samples (fig s1b). J Immunol (2017) ncbi
  • immunocytochemistry; human; 1:100; fig 6
MBL International Pycard antibody (MBL, D086-3) was used in immunocytochemistry on human samples at 1:100 (fig 6). PLoS Pathog (2016) ncbi
Articles Reviewed
  1. Yuan B, Zhou X, You Z, Xu W, Fan J, Chen S, et al. Inhibition of AIM2 inflammasome activation alleviates GSDMD-induced pyroptosis in early brain injury after subarachnoid haemorrhage. Cell Death Dis. 2020;11:76 pubmed publisher
  2. Lim J, Park H, Heisler J, Maculins T, Roose Girma M, Xu M, et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. elife. 2019;8: pubmed publisher
  3. Qiu T, Pei P, Yao X, Jiang L, Wei S, Wang Z, et al. Taurine attenuates arsenic-induced pyroptosis and nonalcoholic steatohepatitis by inhibiting the autophagic-inflammasomal pathway. Cell Death Dis. 2018;9:946 pubmed publisher
  4. Gutierrez K, Davis M, Daniels B, Olsen T, Ralli Jain P, Tait S, et al. MLKL Activation Triggers NLRP3-Mediated Processing and Release of IL-1β Independently of Gasdermin-D. J Immunol. 2017;198:2156-2164 pubmed publisher
  5. Eichholz K, Bru T, Tran T, Fernandes P, Welles H, Mennechet F, et al. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells. PLoS Pathog. 2016;12:e1005871 pubmed publisher
  6. Yao X, Carlson D, Sun Y, Ma L, Wolf S, Minei J, et al. Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model. PLoS ONE. 2015;10:e0139416 pubmed publisher