This is a Validated Antibody Database (VAD) review about mouse Pum1, based on 8 published articles (read how Labome selects the articles), using Pum1 antibody in all methods. It is aimed to help Labome visitors find the most suited Pum1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Pum1 synonym: AA517475; Pumm; mKIAA0099; pumilio homolog 1; pumilio 1

Knockout validation
Abcam
rabbit monoclonal (EPR3795)
  • western blot knockout validation; mouse; 1:3000; loading ...; fig 2c
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1b
Abcam Pum1 antibody (Abcam, 92545) was used in western blot knockout validation on mouse samples at 1:3000 (fig 2c) and in immunohistochemistry on mouse samples at 1:100 (fig 1b). Genes Dev (2017) ncbi
Abcam
rabbit monoclonal (EPR3795)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
Abcam Pum1 antibody (Abcam, ab92545) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1a). Biol Reprod (2016) ncbi
Bethyl
goat polyclonal
  • western blot knockout validation; mouse; 1:5000; fig s2
In order to elucidate how post-translational modification of ATAXIN1 levels contributes to spinocerebellar ataxia type 1, Bethyl Pum1 antibody (Bethyl, A300-201A) was used in western blot knockout validation on mouse samples at 1:5000 (fig s2). Cell (2015) ncbi
Abcam
rabbit monoclonal (EPR3795)
  • western blot; human; 1:4000; loading ...; fig 7s3a
Abcam Pum1 antibody (Abcam, ab92545) was used in western blot on human samples at 1:4000 (fig 7s3a). elife (2019) ncbi
rabbit monoclonal (EPR3795)
  • western blot knockout validation; mouse; 1:3000; loading ...; fig 2c
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1b
Abcam Pum1 antibody (Abcam, 92545) was used in western blot knockout validation on mouse samples at 1:3000 (fig 2c) and in immunohistochemistry on mouse samples at 1:100 (fig 1b). Genes Dev (2017) ncbi
rabbit monoclonal (EPR3795)
  • western blot; human; loading ...; fig 1c
Abcam Pum1 antibody (Abcam, 92545) was used in western blot on human samples (fig 1c). Proc Natl Acad Sci U S A (2017) ncbi
rabbit monoclonal (EPR3795)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
Abcam Pum1 antibody (Abcam, ab92545) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1a). Biol Reprod (2016) ncbi
Bethyl
goat polyclonal
  • western blot; human; fig 3
Bethyl Pum1 antibody (Bethyl, A300-201A) was used in western blot on human samples (fig 3). Nat Commun (2016) ncbi
goat polyclonal
  • western blot; human; fig 7b
Bethyl Pum1 antibody (Bethyl, A300211 201A) was used in western blot on human samples (fig 7b). Mol Cell Biol (2015) ncbi
goat polyclonal
  • western blot knockout validation; mouse; 1:5000; fig s2
In order to elucidate how post-translational modification of ATAXIN1 levels contributes to spinocerebellar ataxia type 1, Bethyl Pum1 antibody (Bethyl, A300-201A) was used in western blot knockout validation on mouse samples at 1:5000 (fig s2). Cell (2015) ncbi
goat polyclonal
  • western blot; human; fig s3
Bethyl Pum1 antibody (Bethyl, A300-201A) was used in western blot on human samples (fig s3). EMBO J (2014) ncbi
Articles Reviewed
  1. Kopp F, Elguindy M, Yalvac M, Zhang H, Chen B, Gillett F, et al. PUMILIO hyperactivity drives premature aging of Norad-deficient mice. elife. 2019;8: pubmed publisher
  2. Zhang M, Chen D, Xia J, Han W, Cui X, Neuenkirchen N, et al. Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins. Genes Dev. 2017;31:1354-1369 pubmed publisher
  3. Liu Y, Qu L, Liu Y, Roizman B, Zhou G. PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2. Proc Natl Acad Sci U S A. 2017;114:E6902-E6911 pubmed publisher
  4. Tichon A, Gil N, Lubelsky Y, Havkin Solomon T, Lemze D, Itzkovitz S, et al. A conserved abundant cytoplasmic long noncoding RNA modulates repression by Pumilio proteins in human cells. Nat Commun. 2016;7:12209 pubmed publisher
  5. Mak W, Fang C, Holden T, Dratver M, Lin H. An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline. Biol Reprod. 2016;94:134 pubmed publisher
  6. Miles W, Lepesant J, Bourdeaux J, Texier M, Kerenyi M, Nakakido M, et al. The LSD1 Family of Histone Demethylases and the Pumilio Posttranscriptional Repressor Function in a Complex Regulatory Feedback Loop. Mol Cell Biol. 2015;35:4199-211 pubmed publisher
  7. Gennarino V, Singh R, White J, De Maio A, Han K, Kim J, et al. Pumilio1 haploinsufficiency leads to SCA1-like neurodegeneration by increasing wild-type Ataxin1 levels. Cell. 2015;160:1087-98 pubmed publisher
  8. Miles W, Korenjak M, Griffiths L, Dyer M, Provero P, Dyson N. Post-transcriptional gene expression control by NANOS is up-regulated and functionally important in pRb-deficient cells. EMBO J. 2014;33:2201-15 pubmed publisher