This is a Validated Antibody Database (VAD) review about mouse Ptpn11, based on 28 published articles (read how Labome selects the articles), using Ptpn11 antibody in all methods. It is aimed to help Labome visitors find the most suited Ptpn11 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ptpn11 synonym: 2700084A17Rik; AW536184; PTP1D; PTP2C; SAP-2; SH-PTP2; SH-PTP3; SHP-2; Shp2; Syp

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (B-1)
  • western blot knockout validation; mouse; 1:500; loading ...; fig 1c
  • immunohistochemistry; mouse; loading ...; fig 4a
  • western blot; human; fig 1e
Santa Cruz Biotechnology Ptpn11 antibody (Santa Cruz, sc-7384) was used in western blot knockout validation on mouse samples at 1:500 (fig 1c), in immunohistochemistry on mouse samples (fig 4a) and in western blot on human samples (fig 1e). Nat Commun (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D50F2)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig s1a
  • western blot; human
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig s2a
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s2a, s2d
Cell Signaling Technology Ptpn11 antibody (Cell Signaling, 3397) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig s1a), in western blot on human samples , in immunohistochemistry knockout validation on mouse samples at 1:200 (fig s2a) and in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig s2a, s2d). Nat Med (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-1)
  • western blot; mouse; 1:500; loading ...; fig 7g
Santa Cruz Biotechnology Ptpn11 antibody (Santa Cruz, sc-7384) was used in western blot on mouse samples at 1:500 (fig 7g). Front Immunol (2018) ncbi
mouse monoclonal (B-1)
  • western blot knockout validation; mouse; 1:500; loading ...; fig 1c
  • immunohistochemistry; mouse; loading ...; fig 4a
  • western blot; human; fig 1e
Santa Cruz Biotechnology Ptpn11 antibody (Santa Cruz, sc-7384) was used in western blot knockout validation on mouse samples at 1:500 (fig 1c), in immunohistochemistry on mouse samples (fig 4a) and in western blot on human samples (fig 1e). Nat Commun (2017) ncbi
mouse monoclonal (B-1)
  • western blot; human; loading ...; fig 4b
Santa Cruz Biotechnology Ptpn11 antibody (SantaCruz, sc-7384) was used in western blot on human samples (fig 4b). Mol Cell Biol (2016) ncbi
mouse monoclonal (B-1)
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig s7c
Santa Cruz Biotechnology Ptpn11 antibody (Santa Cruz, B-1) was used in immunohistochemistry - paraffin section on mouse samples at 1:1000 (fig s7c). Nat Commun (2016) ncbi
mouse monoclonal (B-1)
  • western blot; human; loading ...; fig 1Bb
Santa Cruz Biotechnology Ptpn11 antibody (Santa Cruz Biotechnology, sc-7384) was used in western blot on human samples (fig 1Bb). J Cell Mol Med (2015) ncbi
mouse monoclonal (B-1)
  • western blot; human
In order to study the role of Src homology phosphotyrosyl phosphatase 2 (SHP2) in triple negative breast cancers, Santa Cruz Biotechnology Ptpn11 antibody (Santa Cruz Biotechnology, SC-7384) was used in western blot on human samples . BMC Cancer (2015) ncbi
mouse monoclonal (B-1)
  • western blot; human
Santa Cruz Biotechnology Ptpn11 antibody (Santa Cruz Biotechnology, sc-7384) was used in western blot on human samples . Proteomics (2015) ncbi
mouse monoclonal (B-1)
  • western blot; human
Santa Cruz Biotechnology Ptpn11 antibody (Santa Cruz, sc-7384) was used in western blot on human samples . J Biol Chem (2013) ncbi
Abcam
domestic rabbit monoclonal (EP508(2)Y)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig s17b
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig s14f, s15g
Abcam Ptpn11 antibody (Abcam, ab62322) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig s17b) and in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig s14f, s15g). Nat Med (2018) ncbi
domestic rabbit monoclonal (EP508(2)Y)
  • western blot; human; 1:500; fig 4b
Abcam Ptpn11 antibody (Abcam, ab62322) was used in western blot on human samples at 1:500 (fig 4b). Nat Commun (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 5c
Cell Signaling Technology Ptpn11 antibody (Cell Signaling, 3752) was used in western blot on human samples (fig 5c). Sci Adv (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s1f
Cell Signaling Technology Ptpn11 antibody (Cell Signaling, 3752) was used in western blot on human samples (fig s1f). Cell (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology Ptpn11 antibody (Cell signaling technology, 3752) was used in western blot on human samples (fig 5a). Cell Rep (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...
Cell Signaling Technology Ptpn11 antibody (cell Signaling, 3703) was used in western blot on human samples at 1:500. Nature (2018) ncbi
domestic rabbit monoclonal (D50F2)
  • western blot; human; 1:1000; loading ...
Cell Signaling Technology Ptpn11 antibody (cell Signaling, 3397) was used in western blot on human samples at 1:1000. Nature (2018) ncbi
domestic rabbit monoclonal (D50F2)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig s1a
  • western blot; human
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig s2a
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s2a, s2d
Cell Signaling Technology Ptpn11 antibody (Cell Signaling, 3397) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig s1a), in western blot on human samples , in immunohistochemistry knockout validation on mouse samples at 1:200 (fig s2a) and in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig s2a, s2d). Nat Med (2018) ncbi
domestic rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology Ptpn11 antibody (Cell Signaling, 3751) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit monoclonal (D50F2)
  • western blot; mouse; loading ...; fig s5d
Cell Signaling Technology Ptpn11 antibody (Cell Signaling, 3397T) was used in western blot on mouse samples (fig s5d). J Clin Invest (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig s5d
Cell Signaling Technology Ptpn11 antibody (Cell Signaling, 3751T) was used in western blot on mouse samples (fig s5d). J Clin Invest (2017) ncbi
domestic rabbit monoclonal (D50F2)
  • western blot; mouse; 1:1000; fig 6a
In order to report that CEACAM1 regulates the epithelial-mesenchymal transition via beta-catenin phosphorylation, Cell Signaling Technology Ptpn11 antibody (Cell Signaling Technology, 3397S) was used in western blot on mouse samples at 1:1000 (fig 6a). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D50F2)
  • western blot; mouse; loading ...; fig 2f
Cell Signaling Technology Ptpn11 antibody (Cell Signaling Technology, 3397) was used in western blot on mouse samples (fig 2f). J Exp Med (2016) ncbi
domestic rabbit monoclonal (D50F2)
  • western blot; mouse; 1:1000; fig 1
Cell Signaling Technology Ptpn11 antibody (Cell Signaling, 3397) was used in western blot on mouse samples at 1:1000 (fig 1). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 3
In order to study a novel crizotinib-resistant solvent-front mutation in a patient with ROS1-rearranged lung cancer that is responsive to cabozantinib therapy, Cell Signaling Technology Ptpn11 antibody (Cell signaling, 3703) was used in western blot on mouse samples at 1:1000 (fig 3). Clin Cancer Res (2016) ncbi
domestic rabbit monoclonal (D50F2)
  • western blot; human; 1:1000; loading ...; fig S6a
  • other; mouse; loading ...; fig 6g
  • proximity ligation assay; mouse; loading ...; fig 6j
Cell Signaling Technology Ptpn11 antibody (Cell Signaling Technology, 3397) was used in western blot on human samples at 1:1000 (fig S6a), in other on mouse samples (fig 6g) and in proximity ligation assay on mouse samples (fig 6j). Nat Commun (2015) ncbi
domestic rabbit monoclonal (D66F10)
  • western blot; mouse; 1:1000; fig 4
In order to study phosphorylation events at sites of adhesion, Cell Signaling Technology Ptpn11 antibody (Cell Signaling Technology, 5431S) was used in western blot on mouse samples at 1:1000 (fig 4). Nat Commun (2015) ncbi
domestic rabbit monoclonal (D50F2)
  • western blot; human
Cell Signaling Technology Ptpn11 antibody (Cell Signaling, 3397) was used in western blot on human samples . Am J Physiol Renal Physiol (2013) ncbi
BD Biosciences
mouse monoclonal (79/PTP1D/SHP2)
  • western blot; human; loading ...; fig 5a
BD Biosciences Ptpn11 antibody (BD, 79) was used in western blot on human samples (fig 5a). J Exp Med (2019) ncbi
mouse monoclonal (79/PTP1D/SHP2)
  • western blot; human; loading ...; fig 5c
BD Biosciences Ptpn11 antibody (BD, 610621) was used in western blot on human samples (fig 5c). J Biol Chem (2017) ncbi
mouse monoclonal (79/PTP1D/SHP2)
  • western blot; human; loading ...; fig 5b
  • western blot; mouse; loading ...; fig 8b
BD Biosciences Ptpn11 antibody (BD Biosciences, 610622) was used in western blot on human samples (fig 5b) and in western blot on mouse samples (fig 8b). Redox Biol (2017) ncbi
mouse monoclonal (79/PTP1D/SHP2)
  • immunoprecipitation; mouse; 1:100; loading ...; fig 1e
  • western blot; mouse; 1:1000; loading ...; fig 1c
BD Biosciences Ptpn11 antibody (BD Biosciences, 610622) was used in immunoprecipitation on mouse samples at 1:100 (fig 1e) and in western blot on mouse samples at 1:1000 (fig 1c). JCI Insight (2016) ncbi
mouse monoclonal (79/PTP1D/SHP2)
  • western blot; Xenopus laevis; 1:2500
In order to examine the contribution of the Lhx9-integrin-paxillin pathway to proepicardial organ positioning and epicardial formation, BD Biosciences Ptpn11 antibody (BD Transduction, 610622) was used in western blot on Xenopus laevis samples at 1:2500. Development (2016) ncbi
mouse monoclonal (79/PTP1D/SHP2)
  • western blot; Xenopus laevis; fig s12
In order to investigate the role of cardiac transcription factor CASTOR (CASZ1) in heart development, BD Biosciences Ptpn11 antibody (BD Transduction Laboratories, 610622) was used in western blot on Xenopus laevis samples (fig s12). Development (2014) ncbi
Articles Reviewed
  1. Marasco M, Berteotti A, Weyershaeuser J, Thorausch N, Sikorska J, Krausze J, et al. Molecular mechanism of SHP2 activation by PD-1 stimulation. Sci Adv. 2020;6:eaay4458 pubmed publisher
  2. Lundby A, Franciosa G, Emdal K, Refsgaard J, Gnosa S, Bekker Jensen D, et al. Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites. Cell. 2019;179:543-560.e26 pubmed publisher
  3. Celis Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Gregoire C, et al. Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Cell Rep. 2019;27:3315-3330.e7 pubmed publisher
  4. Fenwick C, Loredo Varela J, Joo V, Pellaton C, Farina A, Rajah N, et al. Tumor suppression of novel anti-PD-1 antibodies mediated through CD28 costimulatory pathway. J Exp Med. 2019;: pubmed publisher
  5. Glal D, Sudhakar J, Lu H, Liu M, Chiang H, Liu Y, et al. ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases. Front Immunol. 2018;9:2522 pubmed publisher
  6. Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, et al. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018;562:605-609 pubmed publisher
  7. Ruess D, Heynen G, Ciecielski K, Ai J, Berninger A, Kabacaoglu D, et al. Mutant KRAS-driven cancers depend on PTPN11/SHP2 phosphatase. Nat Med. 2018;24:954-960 pubmed publisher
  8. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  9. Guo W, Liu W, Chen Z, Gu Y, Peng S, Shen L, et al. Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis. Nat Commun. 2017;8:2168 pubmed publisher
  10. Li B, Wang X, Choi I, Wang Y, Liu S, Pham A, et al. miR-146a modulates autoreactive Th17 cell differentiation and regulates organ-specific autoimmunity. J Clin Invest. 2017;127:3702-3716 pubmed publisher
  11. Juhasz A, Markel S, Gaur S, Liu H, Lu J, Jiang G, et al. NADPH oxidase 1 supports proliferation of colon cancer cells by modulating reactive oxygen species-dependent signal transduction. J Biol Chem. 2017;292:7866-7887 pubmed publisher
  12. Chattopadhyay R, Raghavan S, Rao G. Resolvin D1 via prevention of ROS-mediated SHP2 inactivation protects endothelial adherens junction integrity and barrier function. Redox Biol. 2017;12:438-455 pubmed publisher
  13. Yi J, Huang Y, Kwaczala A, Kuo I, Ehrlich B, Campbell S, et al. Low-dose dasatinib rescues cardiac function in Noonan syndrome. JCI Insight. 2016;1:e90220 pubmed publisher
  14. Sauer M, Herbst J, Diekmann U, Rudd C, Kardinal C. SHP-1 Acts as a Key Regulator of Alloresponses by Modulating LFA-1-Mediated Adhesion in Primary Murine T Cells. Mol Cell Biol. 2016;36:3113-3127 pubmed
  15. Kikuchi I, Takahashi Kanemitsu A, Sakiyama N, Tang C, Tang P, Noda S, et al. Dephosphorylated parafibromin is a transcriptional coactivator of the Wnt/Hedgehog/Notch pathways. Nat Commun. 2016;7:12887 pubmed publisher
  16. Wegwitz F, Lenfert E, Gerstel D, von Ehrenstein L, Einhoff J, Schmidt G, et al. CEACAM1 controls the EMT switch in murine mammary carcinoma in vitro and in vivo. Oncotarget. 2016;7:63730-63746 pubmed publisher
  17. Zhu L, Luo T, Xu X, Guo Y, Zhao X, Wang T, et al. E3 ubiquitin ligase Cbl-b negatively regulates C-type lectin receptor-mediated antifungal innate immunity. J Exp Med. 2016;213:1555-70 pubmed publisher
  18. Uto T, Fukaya T, Takagi H, Arimura K, Nakamura T, Kojima N, et al. Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity. Nat Commun. 2016;7:11273 pubmed publisher
  19. Tandon P, Wilczewski C, Williams C, Conlon F. The Lhx9-integrin pathway is essential for positioning of the proepicardial organ. Development. 2016;143:831-40 pubmed publisher
  20. Drilon A, Somwar R, Wagner J, Vellore N, Eide C, Zabriskie M, et al. A Novel Crizotinib-Resistant Solvent-Front Mutation Responsive to Cabozantinib Therapy in a Patient with ROS1-Rearranged Lung Cancer. Clin Cancer Res. 2016;22:2351-8 pubmed publisher
  21. Wang S, Zheng G, Zhao L, Xu F, Qian J. Shp-2 contributes to anti-RSV activity in human pulmonary alveolar epithelial cells by interfering with the IFN-α-induced Jak/Stat1 pathway. J Cell Mol Med. 2015;19:2432-40 pubmed publisher
  22. Zhao H, Agazie Y. Inhibition of SHP2 in basal-like and triple-negative breast cells induces basal-to-luminal transition, hormone dependency, and sensitivity to anti-hormone treatment. BMC Cancer. 2015;15:109 pubmed publisher
  23. Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, et al. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling. Nat Commun. 2015;6:6576 pubmed publisher
  24. Robertson J, Jacquemet G, Byron A, Jones M, Warwood S, Selley J, et al. Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat Commun. 2015;6:6265 pubmed publisher
  25. Osinalde N, Sánchez Quiles V, Akimov V, Guerra B, Blagoev B, Kratchmarova I. Simultaneous dissection and comparison of IL-2 and IL-15 signaling pathways by global quantitative phosphoproteomics. Proteomics. 2015;15:520-31 pubmed publisher
  26. Sojka S, Amin N, Gibbs D, Christine K, Charpentier M, Conlon F. Congenital heart disease protein 5 associates with CASZ1 to maintain myocardial tissue integrity. Development. 2014;141:3040-9 pubmed publisher
  27. Zhou X, Wang H, Burg M, Ferraris J. High NaCl-induced inhibition of PTG contributes to activation of NFAT5 through attenuation of the negative effect of SHP-1. Am J Physiol Renal Physiol. 2013;305:F362-9 pubmed publisher
  28. Abbonante V, Gruppi C, Rubel D, Gross O, Moratti R, Balduini A. Discoidin domain receptor 1 protein is a novel modulator of megakaryocyte-collagen interactions. J Biol Chem. 2013;288:16738-46 pubmed publisher