This is a Validated Antibody Database (VAD) review about mouse Prpf8, based on 6 published articles (read how Labome selects the articles), using Prpf8 antibody in all methods. It is aimed to help Labome visitors find the most suited Prpf8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Prpf8 synonym: AU019467; D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l; pre-mRNA-processing-splicing factor 8; splicing factor Prp8

Abcam
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 3
Abcam Prpf8 antibody (Abcam, ab79237) was used in immunohistochemistry on mouse samples at 1:100 (fig 3). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5.b
Abcam Prpf8 antibody (Abcam, ab79237) was used in western blot on mouse samples (fig 5.b). Nucleic Acids Res (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-6)
  • western blot; mouse; 1:200; fig 3
Santa Cruz Biotechnology Prpf8 antibody (Santa Cruz, sc-55534) was used in western blot on mouse samples at 1:200 (fig 3). Nat Cell Biol (2015) ncbi
mouse monoclonal (E-5)
  • western blot; human
Santa Cruz Biotechnology Prpf8 antibody (Santa, sc-55533) was used in western blot on human samples . Mol Cell (2014) ncbi
mouse monoclonal (E-5)
  • western blot; Sciuridae
Santa Cruz Biotechnology Prpf8 antibody (Santa Cruz Biotechnology, sc-55533) was used in western blot on Sciuridae samples . Mol Cell Proteomics (2010) ncbi
Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml; fig 1a
In order to develop reagents to study RNA binding proteins, Bethyl Prpf8 antibody (Bethyl, A303-921A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml (fig 1a). Mol Cell (2016) ncbi
Articles Reviewed
  1. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  2. Esquerdo M, Grau Bové X, Garanto A, Toulis V, Garcia Monclús S, Millo E, et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS ONE. 2016;11:e0150364 pubmed publisher
  3. Wheway G, Schmidts M, Mans D, Szymanska K, Nguyen T, Racher H, et al. An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. Nat Cell Biol. 2015;17:1074-1087 pubmed publisher
  4. Van Maldegem F, Maslen S, Johnson C, Chandra A, Ganesh K, Skehel M, et al. CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic Acids Res. 2015;43:7058-69 pubmed publisher
  5. Savage K, Gorski J, Barros E, Irwin G, Manti L, Powell A, et al. Identification of a BRCA1-mRNA splicing complex required for efficient DNA repair and maintenance of genomic stability. Mol Cell. 2014;54:445-59 pubmed publisher
  6. Shao C, Liu Y, Ruan H, Li Y, Wang H, Kohl F, et al. Shotgun proteomics analysis of hibernating arctic ground squirrels. Mol Cell Proteomics. 2010;9:313-26 pubmed publisher