This is a Validated Antibody Database (VAD) review about mouse Prmt5, based on 22 published articles (read how Labome selects the articles), using Prmt5 antibody in all methods. It is aimed to help Labome visitors find the most suited Prmt5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Prmt5 synonym: Jbp1; Skb1; protein arginine N-methyltransferase 5; Jak-binding protein 1; SKB1 homolog; histone-arginine N-methyltransferase PRMT5; shk1 kinase-binding protein 1 homolog

Abcam
rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig 1a, 1b, 6a
  • western blot; human; 1:500; loading ...; fig 3a, 3c, 3d
In order to identify protein arginine methyltransferase 5 as an important modulator of CD4(+) T cell expansion, Abcam Prmt5 antibody (Abcam, ab31751) was used in western blot on mouse samples at 1:500 (fig 1a, 1b, 6a) and in western blot on human samples at 1:500 (fig 3a, 3c, 3d). J Immunol (2017) ncbi
rabbit monoclonal (EPR5772)
  • immunohistochemistry; human; loading ...; fig 1a
  • western blot; human; loading ...; fig 3a
Abcam Prmt5 antibody (Abcam, ab109451) was used in immunohistochemistry on human samples (fig 1a) and in western blot on human samples (fig 3a). Oncotarget (2016) ncbi
rabbit monoclonal (EPR5772)
  • immunocytochemistry; mouse; 1:50; fig 5a
Abcam Prmt5 antibody (Abcam, ab109451) was used in immunocytochemistry on mouse samples at 1:50 (fig 5a). Genome Biol (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:70
  • western blot; human
In order to study the increased immunohistochemical expression of PRMT5 in malignant and metastatic melanoma and the pathological significance, Abcam Prmt5 antibody (Abcam, ab31751) was used in immunohistochemistry - paraffin section on human samples at 1:70 and in western blot on human samples . PLoS ONE (2013) ncbi
rabbit monoclonal (EPR5772)
  • western blot; mouse
Abcam Prmt5 antibody (Abcam, ab109451) was used in western blot on mouse samples . Mol Cancer Res (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
  • western blot; mouse
Abcam Prmt5 antibody (Abcam, ab31751) was used in chromatin immunoprecipitation on mouse samples and in western blot on mouse samples . Mol Cancer Res (2013) ncbi
rabbit monoclonal (EPR5772)
  • western blot; human
In order to investigate the role of menin in MEN1 tumor syndrome, Abcam Prmt5 antibody (Abcam, ab109451) was used in western blot on human samples . Cancer Res (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • immunoprecipitation; human
  • western blot; human
In order to investigate the role of menin in MEN1 tumor syndrome, Abcam Prmt5 antibody (Abcam, ab31751) was used in chromatin immunoprecipitation on human samples , in immunoprecipitation on human samples and in western blot on human samples . Cancer Res (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-11)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2c
  • western blot; mouse; loading ...; fig 2a
Santa Cruz Biotechnology Prmt5 antibody (Santa Cruz Biotechnology Inc, sc-376937) was used in immunohistochemistry - frozen section on mouse samples (fig 2c) and in western blot on mouse samples (fig 2a). J Biol Chem (2018) ncbi
mouse monoclonal (A-11)
  • western blot; human; fig 6c
Santa Cruz Biotechnology Prmt5 antibody (Santa Cruz, sc-376937) was used in western blot on human samples (fig 6c). Nat Commun (2017) ncbi
Proteintech Group
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig s1a
  • western blot; human; 1:1000; loading ...; fig s1a
In order to show that USP9X regulates centrosome biogenesis and plays a role breast carcinogenesis, Proteintech Group Prmt5 antibody (Proteintech, 18436-1-AP) was used in immunoprecipitation on human samples (fig s1a) and in western blot on human samples at 1:1000 (fig s1a). Nat Commun (2017) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 1b, 6a
Cell Signaling Technology Prmt5 antibody (Cell Signaling, 2252) was used in western blot on human samples (fig 1b, 6a). FASEB J (2018) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 1d
In order to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair, Cell Signaling Technology Prmt5 antibody (Cell Signaling Technologies, 2252) was used in western blot on human samples (fig 1d). Mol Cell (2017) ncbi
rabbit polyclonal
  • western blot; human; fig 1
Cell Signaling Technology Prmt5 antibody (CST, 2252) was used in western blot on human samples (fig 1). elife (2016) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; loading ...; fig 1d
In order to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair, EMD Millipore Prmt5 antibody (Millipore, 07-405) was used in western blot on human samples (fig 1d). Mol Cell (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5
  • immunohistochemistry - paraffin section; human; loading ...; fig 4
  • chromatin immunoprecipitation; human; loading ...; fig 3a,3b
  • immunoprecipitation; human; loading ...; fig 3a,3b
  • western blot; human; loading ...; fig 1e,2d
EMD Millipore Prmt5 antibody (Millipore, 07-405) was used in immunohistochemistry - paraffin section on mouse samples (fig 5), in immunohistochemistry - paraffin section on human samples (fig 4), in chromatin immunoprecipitation on human samples (fig 3a,3b), in immunoprecipitation on human samples (fig 3a,3b) and in western blot on human samples (fig 1e,2d). Oncogene (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 1g
  • western blot; human; 1:5000; loading ...; fig s1c
In order to investigate the impact of protein arginine methyltransferase 5 in lung and breast cancer, EMD Millipore Prmt5 antibody (Millipore, 07-405) was used in immunocytochemistry on human samples (fig 1g) and in western blot on human samples at 1:5000 (fig s1c). Oncogene (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 1a
EMD Millipore Prmt5 antibody (Millipore, 07-405) was used in western blot on human samples at 1:1000 (fig 1a). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:200; fig s1
  • western blot; human; 1:2000; fig 1
In order to study the relationship between arginine methylation and ubiquitylation in carcinogenesis and KLF4-mediated stability, EMD Millipore Prmt5 antibody (Millipore, 07-405) was used in immunocytochemistry on human samples at 1:200 (fig s1) and in western blot on human samples at 1:2000 (fig 1). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1b
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2b
EMD Millipore Prmt5 antibody (Millipore, 07-405) was used in immunocytochemistry on mouse samples at 1:200 (fig 1b) and in immunohistochemistry on mouse samples at 1:200 (fig 2b). Sci Rep (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:250; fig s3e
In order to identify SOX17 as a key regulator of human primordial germ cell fate, EMD Millipore Prmt5 antibody (Millipore, 07-405) was used in immunohistochemistry - frozen section on human samples at 1:250 (fig s3e). Cell (2015) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore Prmt5 antibody (Millipore, 07-405) was used in western blot on human samples . Biochim Biophys Acta (2014) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore Prmt5 antibody (Merck Millipore, 07-405) was used in western blot on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:500
EMD Millipore Prmt5 antibody (Millipore, 07-405) was used in immunocytochemistry on human samples at 1:500. Stem Cell Rev (2014) ncbi
Sigma-Aldrich
mouse monoclonal (PRMT5-21)
  • western blot; human; 1:1000; fig s3d
Sigma-Aldrich Prmt5 antibody (Sigma, P0493) was used in western blot on human samples at 1:1000 (fig s3d). BMC Cancer (2015) ncbi
Articles Reviewed
  1. Yang C, Chiu L, Chang C, Chuang H, Tan T. Induction of DUSP14 ubiquitination by PRMT5-mediated arginine methylation. FASEB J. 2018;:fj201800244RR pubmed publisher
  2. Huang L, Liu J, Zhang X, Sibley K, Najjar S, Lee M, et al. Inhibition of protein arginine methyltransferase 5 enhances hepatic mitochondrial biogenesis. J Biol Chem. 2018;293:10884-10894 pubmed publisher
  3. Wang Y, Liu X, Zhou L, Duong D, Bhuripanyo K, Zhao B, et al. Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer. Nat Commun. 2017;8:2232 pubmed publisher
  4. Li X, Song N, Liu L, Liu X, Ding X, Song X, et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun. 2017;8:14866 pubmed publisher
  5. Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
  6. Webb L, Amici S, Jablonski K, Savardekar H, Panfil A, Li L, et al. PRMT5-Selective Inhibitors Suppress Inflammatory T Cell Responses and Experimental Autoimmune Encephalomyelitis. J Immunol. 2017;198:1439-1451 pubmed publisher
  7. Yang H, Zhao X, Zhao L, Liu L, Li J, Jia W, et al. PRMT5 competitively binds to CDK4 to promote G1-S transition upon glucose induction in hepatocellular carcinoma. Oncotarget. 2016;7:72131-72147 pubmed publisher
  8. Deng X, Shao G, Zhang H, Li C, Zhang D, Cheng L, et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene. 2017;36:1223-1231 pubmed publisher
  9. Zhang X, Wu J, Wang J, Shen T, Li H, Lu J, et al. Integrative epigenomic analysis reveals unique epigenetic signatures involved in unipotency of mouse female germline stem cells. Genome Biol. 2016;17:162 pubmed publisher
  10. Chen H, Lorton B, Gupta V, Shechter D. A TGFβ-PRMT5-MEP50 axis regulates cancer cell invasion through histone H3 and H4 arginine methylation coupled transcriptional activation and repression. Oncogene. 2017;36:373-386 pubmed publisher
  11. Mounir Z, Korn J, Westerling T, Lin F, Kirby C, Schirle M, et al. ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. elife. 2016;5: pubmed publisher
  12. Harris D, Chandrasekharan U, Bandyopadhyay S, Willard B, DiCorleto P. PRMT5-Mediated Methylation of NF-κB p65 at Arg174 Is Required for Endothelial CXCL11 Gene Induction in Response to TNF-α and IFN-γ Costimulation. PLoS ONE. 2016;11:e0148905 pubmed publisher
  13. Hu D, Gur M, Zhou Z, Gamper A, Hung M, Fujita N, et al. Interplay between arginine methylation and ubiquitylation regulates KLF4-mediated genome stability and carcinogenesis. Nat Commun. 2015;6:8419 pubmed publisher
  14. Rastetter R, Blömacher M, Drebber U, Marko M, Behrens J, Solga R, et al. Coronin 2A (CRN5) expression is associated with colorectal adenoma-adenocarcinoma sequence and oncogenic signalling. BMC Cancer. 2015;15:638 pubmed publisher
  15. Wang Y, Zhu T, Li Q, Liu C, Han F, Chen M, et al. Prmt5 is required for germ cell survival during spermatogenesis in mice. Sci Rep. 2015;5:11031 pubmed publisher
  16. Irie N, Weinberger L, Tang W, Kobayashi T, Viukov S, Manor Y, et al. SOX17 is a critical specifier of human primordial germ cell fate. Cell. 2015;160:253-68 pubmed publisher
  17. Zhang H, Zhang D, Zha Z, Hu C. Transcriptional activation of PRMT5 by NF-Y is required for cell growth and negatively regulated by the PKC/c-Fos signaling in prostate cancer cells. Biochim Biophys Acta. 2014;1839:1330-40 pubmed publisher
  18. Joliot V, Ait Mohamed O, Battisti V, Pontis J, Philipot O, Robin P, et al. The SWI/SNF subunit/tumor suppressor BAF47/INI1 is essential in cell cycle arrest upon skeletal muscle terminal differentiation. PLoS ONE. 2014;9:e108858 pubmed publisher
  19. Gkountela S, Li Z, Chin C, Lee S, Clark A. PRMT5 is required for human embryonic stem cell proliferation but not pluripotency. Stem Cell Rev. 2014;10:230-9 pubmed publisher
  20. Nicholas C, Yang J, Peters S, Bill M, Baiocchi R, Yan F, et al. PRMT5 is upregulated in malignant and metastatic melanoma and regulates expression of MITF and p27(Kip1.). PLoS ONE. 2013;8:e74710 pubmed publisher
  21. Gurung B, Feng Z, Hua X. Menin directly represses Gli1 expression independent of canonical Hedgehog signaling. Mol Cancer Res. 2013;11:1215-22 pubmed publisher
  22. Gurung B, Feng Z, Iwamoto D, Thiel A, Jin G, Fan C, et al. Menin epigenetically represses Hedgehog signaling in MEN1 tumor syndrome. Cancer Res. 2013;73:2650-8 pubmed publisher