This is a Validated Antibody Database (VAD) review about mouse Prkar2a, based on 26 published articles (read how Labome selects the articles), using Prkar2a antibody in all methods. It is aimed to help Labome visitors find the most suited Prkar2a antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Prkar2a synonym: 1110061A24Rik; AI317181; AI836829; RII(alpha); cAMP-dependent protein kinase type II-alpha regulatory subunit

Knockout validation
BD Biosciences
mouse monoclonal (40/PKA[RII)
  • western blot knockout validation; human; 1:2000; loading ...; fig 3b
  • proximity ligation assay; human
BD Biosciences Prkar2a antibody (BD Biosciences, 40) was used in western blot knockout validation on human samples at 1:2000 (fig 3b) and in proximity ligation assay on human samples . Science (2017) ncbi
BD Biosciences
mouse monoclonal (45)
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot knockout validation; mouse; fig s2
BD Biosciences Prkar2a antibody (BD Bioscience, 610626) was used in immunohistochemistry knockout validation on mouse samples (fig 4) and in western blot knockout validation on mouse samples (fig s2). Nat Commun (2015) ncbi
Abcam
rabbit monoclonal (E151)
  • western blot; mouse; 1:1000; fig 4c
Abcam Prkar2a antibody (Abcam, ab32390) was used in western blot on mouse samples at 1:1000 (fig 4c). Mol Metab (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4c
Abcam Prkar2a antibody (Abcam, ab38949) was used in western blot on mouse samples at 1:1000 (fig 4c). Mol Metab (2017) ncbi
rabbit monoclonal (E151)
  • western blot; pig
Abcam Prkar2a antibody (Abcam, ab32390) was used in western blot on pig samples . PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
Abcam Prkar2a antibody (Abcam, ab38949) was used in western blot on mouse samples at 1:1000. J Neurosci (2014) ncbi
rabbit monoclonal (E151)
  • western blot; mouse
Abcam Prkar2a antibody (Abcam, ab32390) was used in western blot on mouse samples . J Am Heart Assoc (2012) ncbi
Santa Cruz Biotechnology
mouse monoclonal (40)
  • western blot; mouse
Santa Cruz Biotechnology Prkar2a antibody (Santa Cruz, sc-136262) was used in western blot on mouse samples . Biochem Pharmacol (2015) ncbi
BD Biosciences
mouse monoclonal (45)
  • western blot; human; 0.8 ug/ml; loading ...; fig s2d
BD Biosciences Prkar2a antibody (BD Biosciences, 610626) was used in western blot on human samples at 0.8 ug/ml (fig s2d). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (40/PKA[RII)
  • western blot; human; 0.8 ug/ml; loading ...; fig s2d
BD Biosciences Prkar2a antibody (BD Biosciences, 612243) was used in western blot on human samples at 0.8 ug/ml (fig s2d). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (40/PKA[RII)
  • western blot knockout validation; human; 1:2000; loading ...; fig 3b
  • proximity ligation assay; human
BD Biosciences Prkar2a antibody (BD Biosciences, 40) was used in western blot knockout validation on human samples at 1:2000 (fig 3b) and in proximity ligation assay on human samples . Science (2017) ncbi
mouse monoclonal (45)
  • western blot; mouse; loading ...; fig 6a
BD Biosciences Prkar2a antibody (BD Biosciences, 610625) was used in western blot on mouse samples (fig 6a). Diabetes (2017) ncbi
mouse monoclonal (45)
  • immunocytochemistry; mouse; 1:3000; loading ...; fig 1e
BD Biosciences Prkar2a antibody (BD Biosciences, 610625) was used in immunocytochemistry on mouse samples at 1:3000 (fig 1e). PLoS ONE (2017) ncbi
mouse monoclonal (45)
  • western blot; human; 1:1000; fig 2a
BD Biosciences Prkar2a antibody (BD, 610625) was used in western blot on human samples at 1:1000 (fig 2a). Sci Rep (2017) ncbi
mouse monoclonal (40/PKA[RII)
  • immunohistochemistry - paraffin section; human; 1:5000; fig 1j
BD Biosciences Prkar2a antibody (BD, 612243) was used in immunohistochemistry - paraffin section on human samples at 1:5000 (fig 1j). Sci Rep (2017) ncbi
mouse monoclonal (40/PKA[RII)
  • immunohistochemistry; rat; 1:100; loading ...; fig 1
  • western blot; rat; 1:1000; loading ...; fig 2b
In order to evaluate if Ezrin associates with connexin 43 in cell types that form stable gap junctions and serve as an A kinase anchoring protein, BD Biosciences Prkar2a antibody (BD, 612243) was used in immunohistochemistry on rat samples at 1:100 (fig 1) and in western blot on rat samples at 1:1000 (fig 2b). Cell Signal (2017) ncbi
mouse monoclonal (40/PKA[RII)
  • western blot; human; 1:1000; loading ...; fig 4c
In order to elucidate the mechanisms by which ARHGAP36 blocks protein kinase A signaling, BD Biosciences Prkar2a antibody (BD, 612243) was used in western blot on human samples at 1:1000 (fig 4c). Nat Commun (2016) ncbi
mouse monoclonal (45)
  • immunocytochemistry; dog; 1:200; loading ...; fig s3a
  • western blot; human; 1:1000; loading ...; fig 4c
In order to elucidate the mechanisms by which ARHGAP36 blocks protein kinase A signaling, BD Biosciences Prkar2a antibody (BD, 610626) was used in immunocytochemistry on dog samples at 1:200 (fig s3a) and in western blot on human samples at 1:1000 (fig 4c). Nat Commun (2016) ncbi
mouse monoclonal (45)
  • western blot; human; fig 1
BD Biosciences Prkar2a antibody (BD Bioscience, 610626) was used in western blot on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (45)
  • western blot; human; fig 2
BD Biosciences Prkar2a antibody (BD Biosciences, 610626) was used in western blot on human samples (fig 2). Pediatr Res (2016) ncbi
mouse monoclonal (40/PKA[RII)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences Prkar2a antibody (BD, 40) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (45)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences Prkar2a antibody (BD, 45) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (45)
  • immunohistochemistry - free floating section; mouse; 1:3000; loading ...; tbl 1
BD Biosciences Prkar2a antibody (BD Biosciences, 610625) was used in immunohistochemistry - free floating section on mouse samples at 1:3000 (tbl 1). J Comp Neurol (2016) ncbi
mouse monoclonal (45)
  • immunoprecipitation; mouse; 1:500; fig 2b
  • western blot; mouse; fig 2a
BD Biosciences Prkar2a antibody (BD Transduction Laboratories, 45 RUO) was used in immunoprecipitation on mouse samples at 1:500 (fig 2b) and in western blot on mouse samples (fig 2a). Transl Psychiatry (2015) ncbi
mouse monoclonal (45)
  • western blot; human; fig 3
BD Biosciences Prkar2a antibody (BD Biosciences, 610626) was used in western blot on human samples (fig 3). Front Pharmacol (2015) ncbi
mouse monoclonal (40/PKA[RII)
  • western blot; human; fig 3
BD Biosciences Prkar2a antibody (BD Biosciences, 612243) was used in western blot on human samples (fig 3). Front Pharmacol (2015) ncbi
mouse monoclonal (47/PKA)
  • immunohistochemistry - frozen section; mouse; fig 5
  • western blot; mouse; fig 5
BD Biosciences Prkar2a antibody (BD Bioscience, 612550) was used in immunohistochemistry - frozen section on mouse samples (fig 5) and in western blot on mouse samples (fig 5). Nat Commun (2015) ncbi
mouse monoclonal (45)
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot knockout validation; mouse; fig s2
BD Biosciences Prkar2a antibody (BD Bioscience, 610626) was used in immunohistochemistry knockout validation on mouse samples (fig 4) and in western blot knockout validation on mouse samples (fig s2). Nat Commun (2015) ncbi
mouse monoclonal (45)
  • western blot; mouse; 1:1000; fig 4e
BD Biosciences Prkar2a antibody (BD Transduction Laboratories, 610625) was used in western blot on mouse samples at 1:1000 (fig 4e). Mol Metab (2015) ncbi
mouse monoclonal (40/PKA[RII)
  • western blot; mouse; 1:1000; fig 4e
BD Biosciences Prkar2a antibody (BD Transduction Laboratories, 612242) was used in western blot on mouse samples at 1:1000 (fig 4e). Mol Metab (2015) ncbi
mouse monoclonal (45)
  • western blot; human
In order to study PDE3 in human cardiac myocytes, BD Biosciences Prkar2a antibody (BD Biosciences, 610626) was used in western blot on human samples . J Biol Chem (2015) ncbi
mouse monoclonal (40/PKA[RII)
  • immunocytochemistry; human
In order to study ezrin, Cx43 and PKA in cell fusion in cytotrophoblasts, BD Biosciences Prkar2a antibody (BD Biosciences, 612243) was used in immunocytochemistry on human samples . J Cell Sci (2014) ncbi
mouse monoclonal (45)
  • immunohistochemistry; mouse; 1:3,000
BD Biosciences Prkar2a antibody (BD Biosciences, 610626) was used in immunohistochemistry on mouse samples at 1:3,000. Dev Cell (2014) ncbi
mouse monoclonal (40/PKA[RII)
  • immunocytochemistry; mouse; 1:500
BD Biosciences Prkar2a antibody (BD Biosciences, 612242) was used in immunocytochemistry on mouse samples at 1:500. PLoS ONE (2013) ncbi
mouse monoclonal (45)
  • immunocytochemistry; mouse; 1:500
BD Biosciences Prkar2a antibody (BD Biosciences, 610625) was used in immunocytochemistry on mouse samples at 1:500. PLoS ONE (2013) ncbi
mouse monoclonal (45)
  • immunohistochemistry - frozen section; mouse; 1:2000
BD Biosciences Prkar2a antibody (BD Bioscience, 610625) was used in immunohistochemistry - frozen section on mouse samples at 1:2000. J Comp Neurol (2013) ncbi
mouse monoclonal (45)
  • immunohistochemistry; thirteen-lined ground squirrel; 1:500
BD Biosciences Prkar2a antibody (BD Transduction Laboratories, 610625) was used in immunohistochemistry on thirteen-lined ground squirrel samples at 1:500. J Comp Neurol (2012) ncbi
Articles Reviewed
  1. Walker Gray R, Stengel F, Gold M. Mechanisms for restraining cAMP-dependent protein kinase revealed by subunit quantitation and cross-linking approaches. Proc Natl Acad Sci U S A. 2017;114:10414-10419 pubmed publisher
  2. Smith F, Esseltine J, Nygren P, Veesler D, Byrne D, Vonderach M, et al. Local protein kinase A action proceeds through intact holoenzymes. Science. 2017;356:1288-1293 pubmed publisher
  3. Kuo T, Chen T, Lee R, Nguyen N, Broughton A, Zhang D, et al. Pik3r1 Is Required for Glucocorticoid-Induced Perilipin 1 Phosphorylation in Lipid Droplet for Adipocyte Lipolysis. Diabetes. 2017;66:1601-1610 pubmed publisher
  4. Puller C, Arbogast P, Keeley P, Reese B, Haverkamp S. Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors. PLoS ONE. 2017;12:e0173455 pubmed publisher
  5. Weigand I, Ronchi C, Rizk Rabin M, Dalmazi G, Wild V, Bathon K, et al. Differential expression of the protein kinase A subunits in normal adrenal glands and adrenocortical adenomas. Sci Rep. 2017;7:49 pubmed publisher
  6. Cederquist C, Lentucci C, Martinez Calejman C, Hayashi V, Orofino J, GUERTIN D, et al. Systemic insulin sensitivity is regulated by GPS2 inhibition of AKT ubiquitination and activation in adipose tissue. Mol Metab. 2017;6:125-137 pubmed publisher
  7. Dukic A, Haugen L, Pidoux G, Leithe E, Bakke O, Tasken K. A protein kinase A-ezrin complex regulates connexin 43 gap junction communication in liver epithelial cells. Cell Signal. 2017;32:1-11 pubmed publisher
  8. Eccles R, Czajkowski M, Barth C, Müller P, McShane E, Grunwald S, et al. Bimodal antagonism of PKA signalling by ARHGAP36. Nat Commun. 2016;7:12963 pubmed publisher
  9. Bachmann V, Mayrhofer J, Ilouz R, Tschaikner P, Raffeiner P, Röck R, et al. Gpr161 anchoring of PKA consolidates GPCR and cAMP signaling. Proc Natl Acad Sci U S A. 2016;113:7786-91 pubmed publisher
  10. Riggle K, Riehle K, Kenerson H, Turnham R, Homma M, Kazami M, et al. Enhanced cAMP-stimulated protein kinase A activity in human fibrolamellar hepatocellular carcinoma. Pediatr Res. 2016;80:110-8 pubmed publisher
  11. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  12. Farshi P, Fyk Kolodziej B, Krolewski D, Walker P, Ichinose T. Dopamine D1 receptor expression is bipolar cell type-specific in the mouse retina. J Comp Neurol. 2016;524:2059-79 pubmed publisher
  13. Corcoran K, Leaderbrand K, Jovasevic V, Guedea A, Kassam F, Radulovic J. Regulation of fear extinction versus other affective behaviors by discrete cortical scaffolding complexes associated with NR2B and PKA signaling. Transl Psychiatry. 2015;5:e657 pubmed publisher
  14. Gerbaud P, Taskén K, Pidoux G. Spatiotemporal regulation of cAMP signaling controls the human trophoblast fusion. Front Pharmacol. 2015;6:202 pubmed publisher
  15. Yang L, McKnight G. Hypothalamic PKA regulates leptin sensitivity and adiposity. Nat Commun. 2015;6:8237 pubmed publisher
  16. Wolter S, Kloth C, Golombek M, Dittmar F, Försterling L, Seifert R. cCMP causes caspase-dependent apoptosis in mouse lymphoma cell lines. Biochem Pharmacol. 2015;98:119-31 pubmed publisher
  17. Rajan S, Dickson L, Mathew E, Orr C, Ellenbroek J, Philipson L, et al. Chronic hyperglycemia downregulates GLP-1 receptor signaling in pancreatic β-cells via protein kinase A. Mol Metab. 2015;4:265-76 pubmed publisher
  18. Lugenbiel P, Wenz F, Govorov K, Schweizer P, Katus H, Thomas D. Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins. PLoS ONE. 2015;10:e0116395 pubmed publisher
  19. Ahmad F, Shen W, Vandeput F, Szabo Fresnais N, Krall J, Degerman E, et al. Regulation of sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) activity by phosphodiesterase 3A (PDE3A) in human myocardium: phosphorylation-dependent interaction of PDE3A1 with SERCA2. J Biol Chem. 2015;290:6763-76 pubmed publisher
  20. Qiu S, Zhang M, Liu Y, Guo Y, Zhao H, Song Q, et al. GluA1 phosphorylation contributes to postsynaptic amplification of neuropathic pain in the insular cortex. J Neurosci. 2014;34:13505-15 pubmed publisher
  21. Pidoux G, Gerbaud P, Dompierre J, Lygren B, Solstad T, Evain Brion D, et al. A PKA-ezrin-Cx43 signaling complex controls gap junction communication and thereby trophoblast cell fusion. J Cell Sci. 2014;127:4172-85 pubmed publisher
  22. Keeley P, Whitney I, Madsen N, St John A, Borhanian S, Leong S, et al. Independent genomic control of neuronal number across retinal cell types. Dev Cell. 2014;30:103-9 pubmed publisher
  23. Huang Y, Kao J, Tseng D, Chen W, Chiang M, Hwang E. Microtubule-associated type II protein kinase A is important for neurite elongation. PLoS ONE. 2013;8:e73890 pubmed publisher
  24. Lugenbiel P, Bauer A, Kelemen K, Schweizer P, Becker R, Katus H, et al. Biological Heart Rate Reduction Through Genetic Suppression of G?(s) Protein in the Sinoatrial Node. J Am Heart Assoc. 2012;1: pubmed publisher
  25. Kranz K, Dorgau B, Pottek M, Herrling R, Schultz K, Bolte P, et al. Expression of Pannexin1 in the outer plexiform layer of the mouse retina and physiological impact of its knockout. J Comp Neurol. 2013;521:1119-35 pubmed publisher
  26. Light A, Zhu Y, Shi J, Saszik S, Lindstrom S, Davidson L, et al. Organizational motifs for ground squirrel cone bipolar cells. J Comp Neurol. 2012;520:2864-87 pubmed publisher