This is a Validated Antibody Database (VAD) review about mouse Prkaa2, based on 25 published articles (read how Labome selects the articles), using Prkaa2 antibody in all methods. It is aimed to help Labome visitors find the most suited Prkaa2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Prkaa2 synonym: 2310008I11Rik; A830082D05; AMPKalpha2

Knockout validation
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 6d
Abcam Prkaa2 antibody (Abcam, ab3760) was used in western blot knockout validation on mouse samples (fig 6d). Eur J Med Res (2022) ncbi
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 6d
Abcam Prkaa2 antibody (Abcam, ab3760) was used in western blot knockout validation on mouse samples (fig 6d). Eur J Med Res (2022) ncbi
domestic rabbit monoclonal (EPR3052)
  • western blot; mouse; loading ...; fig 4a
  • western blot; rat; loading ...; fig 7f
Abcam Prkaa2 antibody (Abcam, ab109402) was used in western blot on mouse samples (fig 4a) and in western blot on rat samples (fig 7f). Front Pharmacol (2021) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 7f
  • western blot; mouse; loading ...; fig 4a
Abcam Prkaa2 antibody (Abcam, ab3760) was used in western blot on rat samples (fig 7f) and in western blot on mouse samples (fig 4a). Front Pharmacol (2021) ncbi
domestic rabbit monoclonal (EPR5683)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5e
  • western blot; mouse; loading ...; fig 5d
  • western blot; human; loading ...; fig 4a, 5f, s14a
Abcam Prkaa2 antibody (Abcam, ab133448) was used in immunohistochemistry - paraffin section on mouse samples (fig 5e), in western blot on mouse samples (fig 5d) and in western blot on human samples (fig 4a, 5f, s14a). Hepatology (2018) ncbi
domestic rabbit monoclonal (EPMDAR6)
  • other; human; loading ...; fig 4c
Abcam Prkaa2 antibody (Abcam, ab129081) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 4
Abcam Prkaa2 antibody (Abcam, 3760) was used in western blot on human samples (fig 4). Sci Rep (2017) ncbi
domestic rabbit monoclonal (EPR5683)
  • western blot; human; 1:1000; loading ...; fig 5a
Abcam Prkaa2 antibody (Abcam, ab133448) was used in western blot on human samples at 1:1000 (fig 5a). Sci Rep (2017) ncbi
domestic rabbit monoclonal (EPR5683)
  • flow cytometry; mouse; loading ...; fig s5
In order to characterize mitochondrial changes in T cells due to PD-1 blockade therapy, Abcam Prkaa2 antibody (Abcam, EPR5683) was used in flow cytometry on mouse samples (fig s5). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit monoclonal (EPR5683)
  • western blot; mouse; loading ...; fig 7a
Abcam Prkaa2 antibody (Abcam, ab133448) was used in western blot on mouse samples (fig 7a). Cell Death Dis (2016) ncbi
domestic rabbit monoclonal (EPR5683)
  • western blot; mouse; 1:1000; loading ...; fig 3a
In order to report that metformin inhibits advanced glycation end product-induced inflammatory response through AMPK activation and RAGE/NFkappaB pathway suppression, Abcam Prkaa2 antibody (Abcam, ab133448) was used in western blot on mouse samples at 1:1000 (fig 3a). J Diabetes Res (2016) ncbi
domestic rabbit monoclonal (EPR5683)
  • flow cytometry; human; fig 3
Abcam Prkaa2 antibody (Abcam, ab133448) was used in flow cytometry on human samples (fig 3). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3a
Abcam Prkaa2 antibody (Abcam, ab3760) was used in western blot on mouse samples (fig 3a). Nature (2016) ncbi
domestic rabbit monoclonal (EPR5683)
  • western blot; mouse; fig 1
Abcam Prkaa2 antibody (Abcam, ab133448) was used in western blot on mouse samples (fig 1). Aging Cell (2016) ncbi
domestic rabbit monoclonal (EPR5683)
  • western blot; human; 1:1000
Abcam Prkaa2 antibody (Abcam, Ab133448) was used in western blot on human samples at 1:1000. PLoS ONE (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-6)
  • western blot; mouse; 1:500; loading ...; fig 6e
Santa Cruz Biotechnology Prkaa2 antibody (Santa Cruz, sc-74461) was used in western blot on mouse samples at 1:500 (fig 6e). Mol Med Rep (2022) ncbi
mouse monoclonal (D-6)
  • western blot; pigs ; loading ...; fig 5c
Santa Cruz Biotechnology Prkaa2 antibody (Santa Cruz Biotechnology, sc-74461) was used in western blot on pigs samples (fig 5c). Br J Nutr (2017) ncbi
mouse monoclonal (D-6)
  • western blot; rat; fig 2a
In order to elucidate the anti-diabetic activity of ampelopsin, Santa Cruz Biotechnology Prkaa2 antibody (Santa cruz, sc-74461) was used in western blot on rat samples (fig 2a). PLoS ONE (2016) ncbi
mouse monoclonal (D-6)
  • immunocytochemistry; human; 1:200; fig 1
  • western blot; human; 1:200; fig 5
Santa Cruz Biotechnology Prkaa2 antibody (Santa Cruz, sc-74461) was used in immunocytochemistry on human samples at 1:200 (fig 1) and in western blot on human samples at 1:200 (fig 5). Biochim Biophys Acta (2015) ncbi
mouse monoclonal (D-6)
  • western blot; human; 1:1000
Santa Cruz Biotechnology Prkaa2 antibody (Santa Cruz Biotechnology, sc-74461) was used in western blot on human samples at 1:1000. Autophagy (2013) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 6g
Invitrogen Prkaa2 antibody (ThermoFisher, 44-1150G) was used in western blot on human samples at 1:1000 (fig 6g). NPJ Breast Cancer (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 1d, 1e, 1f
Invitrogen Prkaa2 antibody (Thermo Fischer, PA521494) was used in western blot on mouse samples at 1:1000 (fig 1d, 1e, 1f). Basic Res Cardiol (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 6a
Invitrogen Prkaa2 antibody (Thermo Fisher, PA5-17831) was used in western blot on human samples at 1:1000 (fig 6a). Int J Mol Med (2020) ncbi
domestic rabbit polyclonal
  • flow cytometry; human; loading ...; fig 4e, 4f
Invitrogen Prkaa2 antibody (Thermo Fisher Scientific, 44-C1150G) was used in flow cytometry on human samples (fig 4e, 4f). Nat Immunol (2019) ncbi
R&D Systems
domestic goat polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 4g
  • western blot; mouse; 1:1000; loading ...; fig 4e
R&D Systems Prkaa2 antibody (R&D Systems, AF2850) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 4g) and in western blot on mouse samples at 1:1000 (fig 4e). Int J Mol Sci (2021) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 7c
Cell Signaling Technology Prkaa2 antibody (Cell Signaling, 4185) was used in western blot on mouse samples (fig 7c). Autophagy (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 6
Cell Signaling Technology Prkaa2 antibody (Cell Signaling, 4185) was used in western blot on human samples (fig 6). Biosci Rep (2016) ncbi
Articles Reviewed
  1. Yang C, Su H, An N, Wu H, Guo X, Li Z, et al. AMP-activated protein kinase α2 contributes to acute and chronic hyperuricemic nephropathy via renal urate deposition in a mouse model. Eur J Med Res. 2022;27:176 pubmed publisher
  2. Yeh C, Liu H, Lee M, Leu Y, Chiang W, Chang H, et al. Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice. Mol Med Rep. 2022;25: pubmed publisher
  3. Gyamfi J, Yeo J, Kwon D, Min B, Cha Y, Koo J, et al. Interaction between CD36 and FABP4 modulates adipocyte-induced fatty acid import and metabolism in breast cancer. NPJ Breast Cancer. 2021;7:129 pubmed publisher
  4. Mou S, Zhou Z, Feng H, Zhang N, Lin Z, Aiyasiding X, et al. Liquiritin Attenuates Lipopolysaccharides-Induced Cardiomyocyte Injury via an AMP-Activated Protein Kinase-Dependent Signaling Pathway. Front Pharmacol. 2021;12:648688 pubmed publisher
  5. Dufeys C, Daskalopoulos E, Castanares Zapatero D, Conway S, Ginion A, Bouzin C, et al. AMPKα1 deletion in myofibroblasts exacerbates post-myocardial infarction fibrosis by a connexin 43 mechanism. Basic Res Cardiol. 2021;116:10 pubmed publisher
  6. Chan C, Wu S, Bao B, Li H, Lu T. MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake. Int J Mol Sci. 2021;22: pubmed publisher
  7. Mlyczynska E, Kurowska P, Drwal E, Opydo Chanek M, Tworzydło W, Kotula Balak M, et al. Apelin and apelin receptor in human placenta: Expression, signalling pathway and regulation of trophoblast JEG‑3 and BeWo cells proliferation and cell cycle. Int J Mol Med. 2020;45:691-702 pubmed publisher
  8. Wen Z, Jin K, Shen Y, Yang Z, Li Y, Wu B, et al. N-myristoyltransferase deficiency impairs activation of kinase AMPK and promotes synovial tissue inflammation. Nat Immunol. 2019;20:313-325 pubmed publisher
  9. Wang Y, Du L, Liang X, Meng P, Bi L, Wang Y, et al. Sirtuin 4 Depletion Promotes Hepatocellular Carcinoma Tumorigenesis Through Regulating Adenosine-Monophosphate-Activated Protein Kinase Alpha/Mammalian Target of Rapamycin Axis in Mice. Hepatology. 2018;: pubmed publisher
  10. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  11. Chung E, Efstathiou N, Konstantinou E, Maidana D, Miller J, Young L, et al. AICAR suppresses TNF-α-induced complement factor B in RPE cells. Sci Rep. 2017;7:17651 pubmed publisher
  12. Fan Q, Long B, Yan G, Wang Z, Shi M, Bao X, et al. Dietary leucine supplementation alters energy metabolism and induces slow-to-fast transitions in longissimus dorsi muscle of weanling piglets. Br J Nutr. 2017;117:1222-1234 pubmed publisher
  13. Fu S, Xu H, Gu M, Liu C, Wang Q, Wan X, et al. Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity. Sci Rep. 2017;7:43771 pubmed publisher
  14. Liu J, Wang H, Gu J, Deng T, Yuan Z, Hu B, et al. BECN1-dependent CASP2 incomplete autophagy induction by binding to rabies virus phosphoprotein. Autophagy. 2017;13:739-753 pubmed publisher
  15. Chamoto K, Chowdhury P, Kumar A, Sonomura K, Matsuda F, Fagarasan S, et al. Mitochondrial activation chemicals synergize with surface receptor PD-1 blockade for T cell-dependent antitumor activity. Proc Natl Acad Sci U S A. 2017;114:E761-E770 pubmed publisher
  16. Liu Z, Gan L, Wu T, Feng F, Luo D, Gu H, et al. Adiponectin reduces ER stress-induced apoptosis through PPARα transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis. 2016;7:e2487 pubmed publisher
  17. Zhou Z, Tang Y, Jin X, Chen C, Lu Y, Liu L, et al. Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NF?B Pathway Suppression. J Diabetes Res. 2016;2016:4847812 pubmed
  18. Weikel K, Cacicedo J, Ruderman N, Ido Y. Knockdown of GSK3β increases basal autophagy and AMPK signalling in nutrient-laden human aortic endothelial cells. Biosci Rep. 2016;36: pubmed publisher
  19. Mölzer C, Wallner M, Kern C, Tosevska A, Schwarz U, Zadnikar R, et al. Features of an altered AMPK metabolic pathway in Gilbert's Syndrome, and its role in metabolic health. Sci Rep. 2016;6:30051 pubmed publisher
  20. Zhou Y, Wu Y, Qin Y, Liu L, Wan J, Zou L, et al. Ampelopsin Improves Insulin Resistance by Activating PPAR? and Subsequently Up-Regulating FGF21-AMPK Signaling Pathway. PLoS ONE. 2016;11:e0159191 pubmed publisher
  21. Shin H, Kim H, Oh S, Lee J, Kee M, Ko H, et al. AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Nature. 2016;534:553-7 pubmed publisher
  22. Han X, Tai H, Wang X, Wang Z, Zhou J, Wei X, et al. AMPK activation protects cells from oxidative stress-induced senescence via autophagic flux restoration and intracellular NAD(+) elevation. Aging Cell. 2016;15:416-27 pubmed publisher
  23. Mendonsa A, Chalfant M, Gorden L, VanSaun M. Modulation of the leptin receptor mediates tumor growth and migration of pancreatic cancer cells. PLoS ONE. 2015;10:e0126686 pubmed publisher
  24. Mahboubi H, Barisé R, Stochaj U. 5'-AMP-activated protein kinase alpha regulates stress granule biogenesis. Biochim Biophys Acta. 2015;1853:1725-37 pubmed publisher
  25. Chen M, Yi L, Jin X, Liang X, Zhou Y, Zhang T, et al. Resveratrol attenuates vascular endothelial inflammation by inducing autophagy through the cAMP signaling pathway. Autophagy. 2013;9:2033-45 pubmed publisher