This is a Validated Antibody Database (VAD) review about mouse Prdm1, based on 38 published articles (read how Labome selects the articles), using Prdm1 antibody in all methods. It is aimed to help Labome visitors find the most suited Prdm1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Prdm1 synonym: Blimp-1; Blimp1; PRDI-BF1; ZNFPR1A1; b2b1765Clo

Knockout validation
Invitrogen
rat monoclonal (6D3)
  • western blot knockout validation; human; fig 5a
  • immunohistochemistry - frozen section; human; 1:100; fig 4a
  • immunocytochemistry; human; 1:100; fig 2e
In order to identify SOX17 as a key regulator of human primordial germ cell fate, Invitrogen Prdm1 antibody (eBioscience, 14-5963) was used in western blot knockout validation on human samples (fig 5a), in immunohistochemistry - frozen section on human samples at 1:100 (fig 4a) and in immunocytochemistry on human samples at 1:100 (fig 2e). Cell (2015) ncbi
Invitrogen
rat monoclonal (6D3)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2d
Invitrogen Prdm1 antibody (Thermo Fisher, 14-5963-80) was used in immunohistochemistry - paraffin section on mouse samples (fig 2d). Theranostics (2020) ncbi
rat monoclonal (6D3)
  • immunohistochemistry - paraffin section; pigs ; 1:50; loading ...; fig 1a
  • immunohistochemistry - frozen section; human; 1:50; fig 3g
In order to elucidate the inital stage of developmental events from human primordial germ cells, Invitrogen Prdm1 antibody (eBiosciences, 14-5963) was used in immunohistochemistry - paraffin section on pigs samples at 1:50 (fig 1a) and in immunohistochemistry - frozen section on human samples at 1:50 (fig 3g). Nature (2017) ncbi
rat monoclonal (6D3)
  • western blot; mouse; loading ...; fig 3g
In order to ask how the aryl hydrocarbon receptor regulates B cell fate, Invitrogen Prdm1 antibody (eBioscience, 14-5963-82) was used in western blot on mouse samples (fig 3g). J Exp Med (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:40,000; loading ...; fig 8
In order to analyze Blimp-1 and neonatal-Fc-receptor expression during the development of the intestinal epithelial barrier function in rats., Invitrogen Prdm1 antibody (Invitrogen, ThermoFisher Scientific, PA5-20310) was used in immunohistochemistry - paraffin section on rat samples at 1:40,000 (fig 8). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; fig 2
  • immunohistochemistry; mouse; fig 3
In order to study oogonial stem cells from the ovary by FACS-sorting to show they are neither germ cells nor DDX4-positive, Invitrogen Prdm1 antibody (ThermoFisher, PA5-20310) was used in immunocytochemistry on mouse samples (fig 2) and in immunohistochemistry on mouse samples (fig 3). Sci Rep (2016) ncbi
rat monoclonal (6D3)
  • immunohistochemistry; mouse; 1:50
Invitrogen Prdm1 antibody (eBioscience, 14-5963) was used in immunohistochemistry on mouse samples at 1:50. Nature (2016) ncbi
mouse monoclonal (3H2-E8)
  • immunocytochemistry; pigs ; fig 2
In order to elucidate mechanisms that regulate reprogramming of porcine primordial germ cells, Invitrogen Prdm1 antibody (Thermo Scientific, MA1-16874) was used in immunocytochemistry on pigs samples (fig 2). Cell Tissue Res (2016) ncbi
rat monoclonal (6D3)
  • immunohistochemistry - paraffin section; human; fig 7
Invitrogen Prdm1 antibody (eBioscience, 14-5963-82) was used in immunohistochemistry - paraffin section on human samples (fig 7). PLoS Genet (2015) ncbi
rat monoclonal (6D3)
  • western blot; mouse; fig 10B
In order to report a B cell-intrinsic role for Rab7, Invitrogen Prdm1 antibody (eBioscience, 14-5963-82) was used in western blot on mouse samples (fig 10B). J Immunol (2015) ncbi
rat monoclonal (6D3)
  • immunohistochemistry - paraffin section; mouse
Invitrogen Prdm1 antibody (eBioscience, 14-5963) was used in immunohistochemistry - paraffin section on mouse samples . Science (2015) ncbi
rat monoclonal (6D3)
  • western blot knockout validation; human; fig 5a
  • immunohistochemistry - frozen section; human; 1:100; fig 4a
  • immunocytochemistry; human; 1:100; fig 2e
In order to identify SOX17 as a key regulator of human primordial germ cell fate, Invitrogen Prdm1 antibody (eBioscience, 14-5963) was used in western blot knockout validation on human samples (fig 5a), in immunohistochemistry - frozen section on human samples at 1:100 (fig 4a) and in immunocytochemistry on human samples at 1:100 (fig 2e). Cell (2015) ncbi
rat monoclonal (6D3)
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 1
Invitrogen Prdm1 antibody (eBioscience, 14-5963-82) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1). Stem Cell Reports (2014) ncbi
rat monoclonal (6D3)
  • flow cytometry; mouse; fig 3
In order to determine the origin of fibroblasts in skin connective tissue, Invitrogen Prdm1 antibody (eBioscience, 14-5963) was used in flow cytometry on mouse samples (fig 3). Nature (2013) ncbi
rat monoclonal (6D3)
  • immunohistochemistry; mouse; fig s1
Invitrogen Prdm1 antibody (eBiosciences, 14-5963-80) was used in immunohistochemistry on mouse samples (fig s1). EMBO Rep (2013) ncbi
BioLegend
rat monoclonal (5E7)
  • flow cytometry; mouse; loading ...; fig 3g
BioLegend Prdm1 antibody (Biolegend, 150007) was used in flow cytometry on mouse samples (fig 3g). J Clin Invest (2021) ncbi
rat monoclonal (5E7)
  • flow cytometry; mouse; 1:100; fig 4c
BioLegend Prdm1 antibody (Biolegend, 150005) was used in flow cytometry on mouse samples at 1:100 (fig 4c). Nat Commun (2019) ncbi
rat monoclonal (5E7)
  • flow cytometry; mouse; 1:300; loading ...; fig 2g
BioLegend Prdm1 antibody (Biolegend, 150004) was used in flow cytometry on mouse samples at 1:300 (fig 2g). Nat Commun (2019) ncbi
rat monoclonal (5E7)
  • flow cytometry; mouse; 1:100; loading ...; fig s2
BioLegend Prdm1 antibody (Biolegend, 150003) was used in flow cytometry on mouse samples at 1:100 (fig s2). Nat Commun (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (3H2E8)
  • western blot; mouse; loading ...; fig s1b
Santa Cruz Biotechnology Prdm1 antibody (Santa, sc-66015) was used in western blot on mouse samples (fig s1b). Cell Rep (2019) ncbi
rat monoclonal (6D3)
  • western blot; human; loading ...; fig 2c
Santa Cruz Biotechnology Prdm1 antibody (Santa, sc-47732) was used in western blot on human samples (fig 2c). Cell Rep (2018) ncbi
rat monoclonal (6D3)
  • immunohistochemistry; mouse; loading ...; fig 2e
Santa Cruz Biotechnology Prdm1 antibody (Santa, 6D3) was used in immunohistochemistry on mouse samples (fig 2e). J Exp Med (2018) ncbi
rat monoclonal
  • immunohistochemistry; mouse; loading ...; fig 2e
Santa Cruz Biotechnology Prdm1 antibody (Santa, 6D3) was used in immunohistochemistry on mouse samples (fig 2e). J Exp Med (2018) ncbi
rat monoclonal (6D3)
  • immunohistochemistry - frozen section; mouse; 1:100
Santa Cruz Biotechnology Prdm1 antibody (Santa Cruz, sc47732) was used in immunohistochemistry - frozen section on mouse samples at 1:100. Dev Biol (2013) ncbi
Novus Biologicals
mouse monoclonal (3H2-E8)
  • flow cytometry; human; loading ...; fig 2b
Novus Biologicals Prdm1 antibody (Novus, 3h2E8) was used in flow cytometry on human samples (fig 2b). Arthritis Res Ther (2019) ncbi
mouse monoclonal (3H2-E8)
  • western blot; mouse; 1:1000; loading ...; fig s7a
Novus Biologicals Prdm1 antibody (Novus Biologicals, NB600-235SS) was used in western blot on mouse samples at 1:1000 (fig s7a). Nat Commun (2019) ncbi
mouse monoclonal (3H2-E8)
  • flow cytometry; mouse
Novus Biologicals Prdm1 antibody (Novus Biologicals, 3H2-E8) was used in flow cytometry on mouse samples . J Immunol (2014) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C14A4)
  • western blot; mouse; loading ...; fig 5b
Cell Signaling Technology Prdm1 antibody (Cell Signaling, 9115) was used in western blot on mouse samples (fig 5b). Sci Adv (2022) ncbi
domestic rabbit monoclonal (C14A4)
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1c, 1d
  • western blot; human; loading ...; fig s3
Cell Signaling Technology Prdm1 antibody (Cell Signaling, 9115S) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1c, 1d) and in western blot on human samples (fig s3). Cells (2019) ncbi
domestic rabbit monoclonal (C14A4)
  • flow cytometry; mouse; loading ...
In order to demonstrate that DNMT3a directs early CD8 positive T-cell effector and memory fate decisions, Cell Signaling Technology Prdm1 antibody (Cell Signaling, C14A4) was used in flow cytometry on mouse samples . Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit monoclonal (C14A4)
  • western blot; mouse; 1:1000; loading ...; fig 4n
In order to show that Kruppel-like transcription factor 6-B cell leukemia/lymphoma 6 regulates macrophage inflammatory gene expression and function, Cell Signaling Technology Prdm1 antibody (Cell Signaling, 9115S) was used in western blot on mouse samples at 1:1000 (fig 4n). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (C14A4)
  • western blot; human; loading ...; fig 4e
In order to test if modulation of Rho-associated kinase 2 alters the generation of T follicular helper cells, Cell Signaling Technology Prdm1 antibody (Cell Signaling, 9115) was used in western blot on human samples (fig 4e). Sci Signal (2016) ncbi
domestic rabbit monoclonal (C14A4)
  • western blot; human; fig 5
In order to determine the necessity for differentiation-dependent viral life cycle by post-transciptional regulation of KLF4 by high-risk human papillomaviruses, Cell Signaling Technology Prdm1 antibody (Cell Signaling Technology, 9115S) was used in western blot on human samples (fig 5). PLoS Pathog (2016) ncbi
domestic rabbit monoclonal (C14A4)
  • immunocytochemistry; human; fig 3
Cell Signaling Technology Prdm1 antibody (Cell signaling, 9115) was used in immunocytochemistry on human samples (fig 3). Stem Cell Reports (2016) ncbi
domestic rabbit monoclonal (C14A4)
  • western blot; mouse; fig 7
Cell Signaling Technology Prdm1 antibody (Cell Signaling Tech, 9115 S) was used in western blot on mouse samples (fig 7). Cell Death Dis (2015) ncbi
domestic rabbit monoclonal (C14A4)
  • western blot; human; 1:1000; fig 8
Cell Signaling Technology Prdm1 antibody (Cell Signaling, 9115) was used in western blot on human samples at 1:1000 (fig 8). PLoS Pathog (2015) ncbi
domestic rabbit monoclonal (C14A4)
  • western blot; mouse; fig 6
Cell Signaling Technology Prdm1 antibody (Cell Signaling, C14A4) was used in western blot on mouse samples (fig 6). J Immunol (2015) ncbi
domestic rabbit monoclonal (C14A4)
  • flow cytometry; human; 1:100; fig 1c
Cell Signaling Technology Prdm1 antibody (Cell Signaling, 9115) was used in flow cytometry on human samples at 1:100 (fig 1c). EMBO J (2015) ncbi
BD Biosciences
rat monoclonal (5E7)
  • flow cytometry; mouse; loading ...; fig 4b
BD Biosciences Prdm1 antibody (BD Biosciences, 563643) was used in flow cytometry on mouse samples (fig 4b). Cell Rep (2019) ncbi
rat monoclonal (5E7)
  • flow cytometry; mouse; loading ...; fig s2c
BD Biosciences Prdm1 antibody (BD Biosciences, 5E7) was used in flow cytometry on mouse samples (fig s2c). Infect Immun (2018) ncbi
rat monoclonal (5E7)
  • flow cytometry; mouse; loading ...; fig 1c
BD Biosciences Prdm1 antibody (BD Biosciences, 5E7) was used in flow cytometry on mouse samples (fig 1c). Cell Rep (2018) ncbi
Articles Reviewed
  1. Que W, Ma K, Hu X, Guo W, Li X. Combinations of anti-GITR antibody and CD28 superagonist induce permanent allograft acceptance by generating type 1 regulatory T cells. Sci Adv. 2022;8:eabo4413 pubmed publisher
  2. Barker K, Etesami N, Shenoy A, Arafa E, Lyon de Ana C, Smith N, et al. Lung-resident memory B cells protect against bacterial pneumonia. J Clin Invest. 2021;131: pubmed publisher
  3. Ge W, Tan S, Wang S, Li L, Sun X, Shen W, et al. Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development. Theranostics. 2020;10:7581-7598 pubmed publisher
  4. Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
  5. Linnebacher A, Mayer P, Marnet N, Bergmann F, Herpel E, Revia S, et al. Interleukin 21 Receptor/Ligand Interaction Is Linked to Disease Progression in Pancreatic Cancer. Cells. 2019;8: pubmed publisher
  6. Leclerc M, Voilin E, Gros G, Corgnac S, de Montpreville V, Validire P, et al. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1. Nat Commun. 2019;10:3345 pubmed publisher
  7. Ye Y, Liu M, Tang L, Du F, Liu Y, Hao P, et al. Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis. Arthritis Res Ther. 2019;21:92 pubmed publisher
  8. Cao Y, Trillo Tinoco J, Sierra R, Anadon C, Dai W, Mohamed E, et al. ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression. Nat Commun. 2019;10:1280 pubmed publisher
  9. Zhu H, Bhatt B, Sivaprakasam S, Cai Y, Liu S, Kodeboyina S, et al. Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR. Nat Commun. 2019;10:1084 pubmed publisher
  10. Kim C, Hu B, Jadhav R, Jin J, Zhang H, Cavanagh M, et al. Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells. Cell Rep. 2018;25:2148-2162.e5 pubmed publisher
  11. Cho S, Lee H, Yu I, Choi Y, Huang H, Hashemifar S, et al. Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b. Nat Commun. 2018;9:2757 pubmed publisher
  12. Noguchi N, Nakamura R, Hatano S, Yamada H, Sun X, Ohara N, et al. Interleukin-21 Induces Short-Lived Effector CD8+ T Cells but Does Not Inhibit Their Exhaustion after Mycobacterium bovis BCG Infection in Mice. Infect Immun. 2018;86: pubmed publisher
  13. Tanaka S, Pfleger C, Lai J, Roan F, Sun S, Ziegler S. KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners. Cell Rep. 2018;23:796-807 pubmed publisher
  14. Zhang Y, Tech L, George L, Acs A, Durrett R, Hess H, et al. Plasma cell output from germinal centers is regulated by signals from Tfh and stromal cells. J Exp Med. 2018;215:1227-1243 pubmed publisher
  15. Kobayashi T, Zhang H, Tang W, Irie N, Withey S, Klisch D, et al. Principles of early human development and germ cell program from conserved model systems. Nature. 2017;546:416-420 pubmed publisher
  16. Vaidyanathan B, Chaudhry A, Yewdell W, Angeletti D, Yen W, Wheatley A, et al. The aryl hydrocarbon receptor controls cell-fate decisions in B cells. J Exp Med. 2017;214:197-208 pubmed publisher
  17. Arevalo Sureda E, Weström B, Pierzynowski S, Prykhodko O. Maturation of the Intestinal Epithelial Barrier in Neonatal Rats Coincides with Decreased FcRn Expression, Replacement of Vacuolated Enterocytes and Changed Blimp-1 Expression. PLoS ONE. 2016;11:e0164775 pubmed publisher
  18. Ladle B, Li K, Phillips M, Pucsek A, Haile A, Powell J, et al. De novo DNA methylation by DNA methyltransferase 3a controls early effector CD8+ T-cell fate decisions following activation. Proc Natl Acad Sci U S A. 2016;113:10631-6 pubmed publisher
  19. Kim G, Das R, Goduni L, McClellan S, Hazlett L, Mahabeleshwar G. Kruppel-like Factor 6 Promotes Macrophage-mediated Inflammation by Suppressing B Cell Leukemia/Lymphoma 6 Expression. J Biol Chem. 2016;291:21271-21282 pubmed
  20. Weiss J, Chen W, Nyuydzefe M, Trzeciak A, Flynn R, Tonra J, et al. ROCK2 signaling is required to induce a subset of T follicular helper cells through opposing effects on STATs in autoimmune settings. Sci Signal. 2016;9:ra73 pubmed publisher
  21. Gunasekharan V, Li Y, Andrade J, Laimins L. Post-Transcriptional Regulation of KLF4 by High-Risk Human Papillomaviruses Is Necessary for the Differentiation-Dependent Viral Life Cycle. PLoS Pathog. 2016;12:e1005747 pubmed publisher
  22. Zarate Garcia L, Lane S, Merriman J, Jones K. FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells. Sci Rep. 2016;6:27991 pubmed publisher
  23. Kushwaha R, Jagadish N, Kustagi M, Mendiratta G, Seandel M, Soni R, et al. Mechanism and Role of SOX2 Repression in Seminoma: Relevance to Human Germline Specification. Stem Cell Reports. 2016;6:772-783 pubmed publisher
  24. Murakami K, Günesdogan U, Zylicz J, Tang W, Sengupta R, Kobayashi T, et al. NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. Nature. 2016;529:403-407 pubmed publisher
  25. Zhang Y, Ma J, Li H, Lv J, Wei R, Cong Y, et al. bFGF signaling-mediated reprogramming of porcine primordial germ cells. Cell Tissue Res. 2016;364:429-41 pubmed publisher
  26. Cao J, Zhang X, Wang Q, Qiu G, Hou C, Wang J, et al. Smad4 represses the generation of memory-precursor effector T cells but is required for the differentiation of central memory T cells. Cell Death Dis. 2015;6:e1984 pubmed publisher
  27. Nawandar D, Wang A, Makielski K, Lee D, Ma S, Barlow E, et al. Differentiation-Dependent KLF4 Expression Promotes Lytic Epstein-Barr Virus Infection in Epithelial Cells. PLoS Pathog. 2015;11:e1005195 pubmed publisher
  28. Mould A, Morgan M, Nelson A, Bikoff E, Robertson E. Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation. PLoS Genet. 2015;11:e1005375 pubmed publisher
  29. Rozanski C, Utley A, Carlson L, Farren M, Murray M, Russell L, et al. CD28 Promotes Plasma Cell Survival, Sustained Antibody Responses, and BLIMP-1 Upregulation through Its Distal PYAP Proline Motif. J Immunol. 2015;194:4717-28 pubmed publisher
  30. Sugawa F, Araúzo Bravo M, Yoon J, Kim K, Aramaki S, Wu G, et al. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. EMBO J. 2015;34:1009-24 pubmed publisher
  31. Pone E, Lam T, Lou Z, Wang R, Chen Y, Liu D, et al. B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses. J Immunol. 2015;194:3065-78 pubmed publisher
  32. Geula S, Moshitch Moshkovitz S, Dominissini D, Mansour A, Kol N, Salmon Divon M, et al. Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation. Science. 2015;347:1002-6 pubmed publisher
  33. Irie N, Weinberger L, Tang W, Kobayashi T, Viukov S, Manor Y, et al. SOX17 is a critical specifier of human primordial germ cell fate. Cell. 2015;160:253-68 pubmed publisher
  34. Kretzschmar K, Cottle D, Donati G, Chiang M, Quist S, Gollnick H, et al. BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions. Stem Cell Reports. 2014;3:620-33 pubmed publisher
  35. Kim E, Gasper D, Lee S, Plisch E, Svaren J, Suresh M. Bach2 regulates homeostasis of Foxp3+ regulatory T cells and protects against fatal lung disease in mice. J Immunol. 2014;192:985-95 pubmed publisher
  36. Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, et al. Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature. 2013;504:277-281 pubmed publisher
  37. Brzezinski J, Uoon Park K, Reh T. Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence. Dev Biol. 2013;384:194-204 pubmed publisher
  38. Grabole N, Tischler J, Hackett J, Kim S, Tang F, Leitch H, et al. Prdm14 promotes germline fate and naive pluripotency by repressing FGF signalling and DNA methylation. EMBO Rep. 2013;14:629-37 pubmed publisher