This is a Validated Antibody Database (VAD) review about mouse Ppp2r2a, based on 12 published articles (read how Labome selects the articles), using Ppp2r2a antibody in all methods. It is aimed to help Labome visitors find the most suited Ppp2r2a antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ppp2r2a synonym: 2410004D02Rik; mKIAA1541

Knockout validation
Cell Signaling Technology
mouse monoclonal (2G9)
  • western blot knockout validation; human; 1:100; loading ...; fig 7a
Cell Signaling Technology Ppp2r2a antibody (Cell Signaling Technology, 5689) was used in western blot knockout validation on human samples at 1:100 (fig 7a). elife (2020) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (100C1)
  • western blot knockout validation; fruit fly ; fig 4b
In order to determine the function of greatwall-phosphorylated endosulfine as a substrate of PP2A-B55 heterotrimers and an inhibitor, Cell Signaling Technology Ppp2r2a antibody (Cell Signaling Technology, 2290) was used in western blot knockout validation on fruit fly samples (fig 4b). elife (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (2G9)
  • immunohistochemistry; human; 1:50; fig 2
In order to test if assessing expression of both PPP2R2A and Cyclin D1 defines a subgroup of luminal breast cancer with poor survival, Santa Cruz Biotechnology Ppp2r2a antibody (Santa Cruz, sc-81606) was used in immunohistochemistry on human samples at 1:50 (fig 2). BMC Cancer (2015) ncbi
mouse monoclonal (2G9)
  • western blot; mouse; 1:500
In order to study the interaction between F-box protein FBXL16 and PP2A-B55alpha and its effect on the differentiation of embryonic stem cells, Santa Cruz Biotechnology Ppp2r2a antibody (Santa Cruz Biotechnology, sc-81606) was used in western blot on mouse samples at 1:500. Mol Cell Proteomics (2014) ncbi
MilliporeSigma
mouse monoclonal (2G9)
  • western blot; human; 1:4000; fig 5
  • western blot; mouse; 1:4000; fig 4
MilliporeSigma Ppp2r2a antibody (Sigma, SAB4200241) was used in western blot on human samples at 1:4000 (fig 5) and in western blot on mouse samples at 1:4000 (fig 4). Nat Commun (2015) ncbi
Cell Signaling Technology
mouse monoclonal (2G9)
  • western blot knockout validation; human; 1:100; loading ...; fig 7a
Cell Signaling Technology Ppp2r2a antibody (Cell Signaling Technology, 5689) was used in western blot knockout validation on human samples at 1:100 (fig 7a). elife (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s1e
Cell Signaling Technology Ppp2r2a antibody (Cell Signaling Technology, 4953) was used in western blot on mouse samples at 1:1000 (fig s1e). J Clin Invest (2019) ncbi
mouse monoclonal (2G9)
  • western blot; human; loading ...; fig 1f
Cell Signaling Technology Ppp2r2a antibody (CST, 5689) was used in western blot on human samples (fig 1f). Nat Cell Biol (2017) ncbi
mouse monoclonal (2G9)
  • western blot; human; loading ...; fig s1a
Cell Signaling Technology Ppp2r2a antibody (Cell Signaling, 5689S) was used in western blot on human samples (fig s1a). J Cell Biol (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 7
Cell Signaling Technology Ppp2r2a antibody (Cell Signaling, 4953) was used in immunocytochemistry on human samples (fig 7). MBio (2016) ncbi
domestic rabbit monoclonal (100C1)
  • western blot; human; 1:1000; fig s7
  • western blot; mouse; 1:1000; fig 3
Cell Signaling Technology Ppp2r2a antibody (Cell Signaling, 2290) was used in western blot on human samples at 1:1000 (fig s7) and in western blot on mouse samples at 1:1000 (fig 3). Nat Commun (2015) ncbi
domestic rabbit monoclonal (100C1)
  • western blot knockout validation; fruit fly ; fig 4b
In order to determine the function of greatwall-phosphorylated endosulfine as a substrate of PP2A-B55 heterotrimers and an inhibitor, Cell Signaling Technology Ppp2r2a antibody (Cell Signaling Technology, 2290) was used in western blot knockout validation on fruit fly samples (fig 4b). elife (2014) ncbi
domestic rabbit monoclonal (100C1)
  • western blot; rat
Cell Signaling Technology Ppp2r2a antibody (Cell Signaling, 100C1) was used in western blot on rat samples . Mol Cell Biol (2013) ncbi
mouse monoclonal (2G9)
  • western blot; rat
Cell Signaling Technology Ppp2r2a antibody (Cell Signaling, 5689) was used in western blot on rat samples . Carcinogenesis (2012) ncbi
Articles Reviewed
  1. Pan L, Lemieux M, Thomas T, Rogers J, Lipper C, Lee W, et al. IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiation. elife. 2020;9: pubmed publisher
  2. Beckelman B, Yang W, Kasica N, Zimmermann H, Zhou X, Keene C, et al. Genetic reduction of eEF2 kinase alleviates pathophysiology in Alzheimer's disease model mice. J Clin Invest. 2019;129:820-833 pubmed publisher
  3. Hein J, Hertz E, Garvanska D, Kruse T, Nilsson J. Distinct kinetics of serine and threonine dephosphorylation are essential for mitosis. Nat Cell Biol. 2017;19:1433-1440 pubmed publisher
  4. Cundell M, Hutter L, Nunes Bastos R, Poser E, Holder J, Mohammed S, et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol. 2016;214:539-54 pubmed publisher
  5. Franco M, Panas M, Marino N, Lee M, Buchholz K, Kelly F, et al. A Novel Secreted Protein, MYR1, Is Central to Toxoplasma's Manipulation of Host Cells. MBio. 2016;7:e02231-15 pubmed publisher
  6. Wong P, Feng Y, Wang J, Shi R, Jiang X. Regulation of autophagy by coordinated action of mTORC1 and protein phosphatase 2A. Nat Commun. 2015;6:8048 pubmed publisher
  7. Yu C, Ji S, Sha Q, Sun Q, Fan H. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun. 2015;6:8017 pubmed publisher
  8. Beca F, Pereira M, Cameselle Teijeiro J, Martins D, Schmitt F. Altered PPP2R2A and Cyclin D1 expression defines a subgroup of aggressive luminal-like breast cancer. BMC Cancer. 2015;15:285 pubmed publisher
  9. Williams B, Filter J, Blake Hodek K, Wadzinski B, Fuda N, Shalloway D, et al. Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers. elife. 2014;3:e01695 pubmed publisher
  10. Honarpour N, Rose C, Brumbaugh J, Anderson J, Graham R, Sweredoski M, et al. F-box protein FBXL16 binds PP2A-B55? and regulates differentiation of embryonic stem cells along the FLK1+ lineage. Mol Cell Proteomics. 2014;13:780-91 pubmed publisher
  11. Kolupaeva V, Daempfling L, Basilico C. The B55? regulatory subunit of protein phosphatase 2A mediates fibroblast growth factor-induced p107 dephosphorylation and growth arrest in chondrocytes. Mol Cell Biol. 2013;33:2865-78 pubmed publisher
  12. Alder H, Taccioli C, Chen H, Jiang Y, Smalley K, Fadda P, et al. Dysregulation of miR-31 and miR-21 induced by zinc deficiency promotes esophageal cancer. Carcinogenesis. 2012;33:1736-44 pubmed publisher